##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513121_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2388446
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845503310520733	31.0	31.0	34.0	27.0	34.0
2	30.936884066041266	33.0	31.0	34.0	27.0	34.0
3	31.041150187192844	33.0	31.0	34.0	27.0	34.0
4	34.43632931202966	37.0	35.0	37.0	31.0	37.0
5	34.20208914080536	37.0	35.0	37.0	30.0	37.0
6	34.12525424481022	37.0	35.0	37.0	30.0	37.0
7	33.994175710901565	37.0	35.0	37.0	30.0	37.0
8	33.959271007173705	37.0	35.0	37.0	30.0	37.0
9	35.51888131446137	39.0	36.0	39.0	30.0	39.0
10	35.362781490559136	39.0	35.0	39.0	29.0	39.0
11	35.377524130752796	39.0	35.0	39.0	30.0	39.0
12	35.31882906291371	39.0	35.0	39.0	29.0	39.0
13	35.29371021995055	39.0	35.0	39.0	29.0	39.0
14	36.456078973525045	40.0	37.0	41.0	29.0	41.0
15	36.44144351599324	40.0	37.0	41.0	29.0	41.0
16	36.412139943712354	40.0	37.0	41.0	29.0	41.0
17	36.34687658837587	40.0	36.0	41.0	28.0	41.0
18	36.29682312264962	40.0	36.0	41.0	28.0	41.0
19	36.377800042370644	40.0	37.0	41.0	28.0	41.0
20	36.343187578869276	40.0	37.0	41.0	28.0	41.0
21	36.29421263867803	40.0	37.0	41.0	28.0	41.0
22	36.23714624488056	40.0	37.0	41.0	28.0	41.0
23	36.16063540896466	40.0	37.0	41.0	28.0	41.0
24	36.10401030628283	40.0	37.0	41.0	28.0	41.0
25	36.05202294713801	40.0	37.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4303621766730714
1101	2	0.4687248807306439
1101	3	0.48154387431050694
1101	4	0.5423169138488788
1101	5	0.4767596345971228
1101	6	0.44852535346370104
1101	7	0.4714947538622525
1101	8	0.48029944931176516
1101	9	0.537158541442011
1101	10	0.6266137688975348
1101	11	0.5797385023404331
1101	12	0.6116268815519348
1101	13	0.561470149193994
1101	14	0.8283917295699439
1101	15	0.8427656265888714
1101	16	0.747917837273981
1101	17	0.7731882670177868
1101	18	0.6637062905449369
1101	19	0.7040952843427917
1101	20	0.7471660177135604
1101	21	0.7597808130498507
1101	22	0.6486376378587977
1101	23	0.7049503893135238
1101	24	0.7083118490542617
1101	25	0.7332112651177312
1102	1	0.2951098570428776
1102	2	0.43213415473050176
1102	3	0.3953142081601868
1102	4	0.42364295681124986
1102	5	0.4150597790775734
1102	6	0.4177723974539518
1102	7	0.4537624627639687
1102	8	0.4417304368208903
1102	9	0.41857743554179905
1102	10	0.5908765983660231
1102	11	0.5909244350556193
1102	12	0.525638958007896
1102	13	0.5219763142583531
1102	14	0.5876512562693179
1102	15	0.6140562432711221
1102	16	0.6167774965864368
1102	17	0.7196652766680245
1102	18	0.6633408510725758
1102	19	0.6525364526691106
1102	20	0.641298689195402
1102	21	0.6406888296742039
1102	22	0.6466708105955092
1102	23	0.7035268550115035
1102	24	0.6808827753930942
1102	25	0.7341933836996972
1103	1	0.297851360721463
1103	2	0.3343403748181899
1103	3	0.3278382108454352
1103	4	0.4537389055658494
1103	5	0.4365964127323494
1103	6	0.2511825422727867
1103	7	0.32198835609652576
1103	8	0.43521279889959175
1103	9	0.40439796816617246
1103	10	0.4425402669569536
1103	11	0.430060264002897
1103	12	0.37928030281533154
1103	13	0.5089380940615698
1103	14	0.463503026941936
1103	15	0.5269224202038671
1103	16	0.3807496969240205
1103	17	0.5495886087843616
1103	18	0.5280883773501657
1103	19	0.45964979542851125
1103	20	0.5422201635792092
1103	21	0.3874345434693396
1103	22	0.5829668409242146
1103	23	0.6072653015206484
1103	24	0.5804696825008833
1103	25	0.5803416570374225
1104	1	0.31877051198980055
1104	2	0.32610450445695705
1104	3	0.40432203727017324
1104	4	0.5211214916581497
1104	5	0.46808139056625464
1104	6	0.4499436057241297
1104	7	0.4702041553643781
1104	8	0.5099879543827228
1104	9	0.5785037466805889
1104	10	0.689886430619886
1104	11	0.6852517624935075
1104	12	0.6348944655615014
1104	13	0.6319414246091171
1104	14	0.684172837492433
1104	15	0.554558345948152
1104	16	0.74765486736338
1104	17	0.7036848219855685
1104	18	0.6154644643946696
1104	19	0.6797038574620586
1104	20	0.7645125525734997
1104	21	0.6921544944777196
1104	22	0.5745027942508543
1104	23	0.48944966782488564
1104	24	0.5066147965527819
1104	25	0.48934968508486065
1105	1	0.26946859852165517
1105	2	0.3095667561616331
1105	3	0.1525629063085603
1105	4	0.31730306100863714
1105	5	0.2960462645738673
1105	6	0.2499720818199691
1105	7	0.21555147813496234
1105	8	0.337929821316159
1105	9	0.43193915602407884
1105	10	0.3967071295190223
1105	11	0.3850565432085915
1105	12	0.324735943721862
1105	13	0.4251673208450413
1105	14	0.46500411794146146
1105	15	0.5165180466950616
1105	16	0.41904536922203306
1105	17	0.42933165967871645
1105	18	0.4288776351157608
1105	19	0.3683108550365546
1105	20	0.3690486025284443
1105	21	0.38783667088186746
1105	22	0.37622318114463127
1105	23	0.3018973160797245
1105	24	0.3044365301505394
1105	25	0.4013951123591539
1106	1	0.289321645230352
1106	2	0.23285342158946065
1106	3	0.24148755025615998
1106	4	0.30210267908417876
1106	5	0.30820635860463597
1106	6	0.2492205457373231
1106	7	0.17846824489060964
1106	8	0.31156230362873316
1106	9	0.36929213852474874
1106	10	0.3613340204454474
1106	11	0.41032942732745425
1106	12	0.3548924904479307
1106	13	0.21936699421502937
1106	14	0.24929279119209724
1106	15	0.417093350625656
1106	16	0.40495888327452434
1106	17	0.3208095925125889
1106	18	0.3334432035985344
1106	19	0.3673343642394542
1106	20	0.41253866388110083
1106	21	0.34503303111760886
1106	22	0.3897285466696019
1106	23	0.18330614596772676
1106	24	0.26084766397366366
1106	25	0.39384658997472144
1107	1	0.1986804239471347
1107	2	0.20306768754339544
1107	3	0.27060457700742546
1107	4	0.37439118701615826
1107	5	0.23303806627721713
1107	6	0.1690781479695218
1107	7	0.2487875169277629
1107	8	0.2375209675230039
1107	9	0.3473635664587462
1107	10	0.35088204808614165
1107	11	0.3726970914031753
1107	12	0.2550714172441104
1107	13	0.29513944335549525
1107	14	0.3671024773339724
1107	15	0.30298118747059277
1107	16	0.3892525909411617
1107	17	0.34867770130245646
1107	18	0.42439275010908517
1107	19	0.44229576781084745
1107	20	0.39494330318669313
1107	21	0.328141925103111
1107	22	0.3258091425568139
1107	23	0.27887893481626236
1107	24	0.31934006813702354
1107	25	0.3973074774397318
1108	1	0.18380453301084643
1108	2	0.2801612283502557
1108	3	0.27239776902785806
1108	4	0.35112592718582647
1108	5	0.19363776537905153
1108	6	0.20206456301166043
1108	7	0.13461530471565197
1108	8	0.2744669764927252
1108	9	0.2593425935527023
1108	10	0.30741102358260264
1108	11	0.2992658892278399
1108	12	0.33089548399448176
1108	13	0.32290121495281454
1108	14	0.35075225617337225
1108	15	0.3360527748935169
1108	16	0.29254543629893703
1108	17	0.30176733494618446
1108	18	0.462784993478806
1108	19	0.5208284637548886
1108	20	0.5307798294396235
1108	21	0.39974535010945544
1108	22	0.3321360124740309
1108	23	0.3819586058724127
1108	24	0.4284742049765171
1108	25	0.4437203043560345
1109	1	0.11926686688827104
1109	2	0.1864247706767621
1109	3	0.07053698578107159
1109	4	0.20405612342823076
1109	5	0.1171185218595241
1109	6	0.14633095851853994
1109	7	0.1371028813609385
1109	8	0.18378136061630812
1109	9	0.09379839901267673
1109	10	0.12536154908463715
1109	11	0.10229235253839875
1109	12	0.09966421520901747
1109	13	0.17280021048413374
1109	14	0.2664789767233131
1109	15	0.24600405985087548
1109	16	0.22243661659064884
1109	17	0.2624897237906936
1109	18	0.23364914167763828
1109	19	0.20947370182508251
1109	20	0.27094518090141406
1109	21	0.19871729005951977
1109	22	0.12105554162055654
1109	23	0.17380480395769382
1109	24	-0.04498366265035969
1109	25	0.05734784889734357
1110	1	-0.04101280733400614
1110	2	-0.06541506218168891
1110	3	-0.007389926865439378
1110	4	-0.017249044751046938
1110	5	-0.10662674429878649
1110	6	0.002859143845604706
1110	7	-0.05451176257092527
1110	8	0.004312539545075822
1110	9	-0.027311763421842272
1110	10	-0.01074555468567695
1110	11	-0.017603067679900164
1110	12	-0.02748839923100377
1110	13	0.03656145511875053
1110	14	-0.009734575103692578
1110	15	-0.06499895301514158
1110	16	0.016015130119832577
1110	17	-0.08708756990758815
1110	18	0.05434868344140398
1110	19	0.1500005481005786
1110	20	-0.07849980694207659
1110	21	-0.08482629690486476
1110	22	6.103251496440976E-4
1110	23	-0.09569400765222014
1110	24	-0.031874042524457025
1110	25	0.03489932550701269
1111	1	0.10628874949090417
1111	2	0.1578877106626777
1111	3	0.17052331974650414
1111	4	0.30526598215604395
1111	5	0.14067321250052345
1111	6	0.1995993583557052
1111	7	0.1928500618849327
1111	8	0.2570579168044418
1111	9	0.21836470073492364
1111	10	0.21259455208936373
1111	11	0.22614376560277094
1111	12	0.2669589785937063
1111	13	0.25148116050070257
1111	14	0.34749887396483103
1111	15	0.32549689491843026
1111	16	0.4153740531959329
1111	17	0.3972785799508145
1111	18	0.3962890878212022
1111	19	0.36456184774804257
1111	20	0.39691046628895776
1111	21	0.3623147240181339
1111	22	0.4241776355599427
1111	23	0.3961901291606367
1111	24	0.37844612259818433
1111	25	0.44161943302873397
1112	1	-0.23765767499533297
1112	2	-0.1757491395529307
1112	3	-0.018045985553062138
1112	4	-0.936737761626496
1112	5	-0.29455222737953335
1112	6	-0.07538441906621074
1112	7	0.0051068491094525825
1112	8	0.05744351777362766
1112	9	0.04027769879684229
1112	10	0.1254800991031857
1112	11	0.06289175378726242
1112	12	0.10292367883931064
1112	13	0.07164247983154581
1112	14	0.10404919848833316
1112	15	0.1684488142184719
1112	16	0.24549135934152133
1112	17	0.19161902244231044
1112	18	0.2532071001345386
1112	19	0.07475315187065945
1112	20	0.12723740699129849
1112	21	0.09728009906543633
1112	22	0.07466747828366493
1112	23	0.11447588825499366
1112	24	0.10005279809914214
1112	25	0.17543786101416003
1113	1	-0.4992070403689297
1113	2	-0.32019972546007125
1113	3	-0.42174714159158455
1113	4	-1.178765202171597
1113	5	-0.5279589682696155
1113	6	-0.33149375669302117
1113	7	-0.2560524721616133
1113	8	-0.23925824609570157
1113	9	-0.09489766735161709
1113	10	-0.08391761480338289
1113	11	-0.1839243843527285
1113	12	-0.0800348448278072
1113	13	-0.10352444164460195
1113	14	-0.13485962921019734
1113	15	-0.017191878894244894
1113	16	-0.0737726433867607
1113	17	-0.12677673341386253
1113	18	-0.1034010879496563
1113	19	-0.1408290085270778
1113	20	-0.13076676198566872
1113	21	-0.08622274637153993
1113	22	-0.05379071942838465
1113	23	-0.07751106890221138
1113	24	-0.14243921542161786
1113	25	-0.030970578090240508
1114	1	-0.4163443943647387
1114	2	-0.2615298087249691
1114	3	-0.25529646837682307
1114	4	-1.0723575914524162
1114	5	-0.4736354401949754
1114	6	-0.4545463627998103
1114	7	-0.35298387371549467
1114	8	-0.29245543353869863
1114	9	-0.2742848569065899
1114	10	-0.24047104987614887
1114	11	-0.05596099639376462
1114	12	-0.22733003037980382
1114	13	-0.17008924514715318
1114	14	-0.09693134210540677
1114	15	-0.16510677478866143
1114	16	-0.23492286894261838
1114	17	-0.2712042030620765
1114	18	-0.2587542343661724
1114	19	-0.37148584538162055
1114	20	-0.42712361328452886
1114	21	-0.3530230469975919
1114	22	-0.29221627103173375
1114	23	-0.29081371428489433
1114	24	-0.21865157458228168
1114	25	-0.20206400753490783
1115	1	0.055268058063067116
1115	2	0.08747002656037495
1115	3	0.08721714658966917
1115	4	0.2985158643477135
1115	5	0.1252167587456512
1115	6	0.15771305411031733
1115	7	0.06630043244570061
1115	8	-6.587434363112266E-4
1115	9	-0.06734561927535765
1115	10	-0.02233634796633055
1115	11	0.12569977811187272
1115	12	0.07241020594237568
1115	13	0.18119953431726543
1115	14	0.06164392815666275
1115	15	0.02635899490557847
1115	16	0.047602986923386936
1115	17	-0.02335081644211101
1115	18	-0.1522165883986446
1115	19	-0.20573914184198827
1115	20	-0.10913417236073997
1115	21	-0.08776795601442444
1115	22	-0.02749866997062611
1115	23	-0.12784774086463813
1115	24	-0.03135998629955594
1115	25	-0.08964115901170544
1116	1	0.14057424711972288
1116	2	0.08913552182224649
1116	3	0.12485475433871329
1116	4	0.25961397959778765
1116	5	0.15556341377905625
1116	6	0.06753517831154454
1116	7	-0.007347095890182231
1116	8	0.03512710760946902
1116	9	0.0071881696940465645
1116	10	-0.013918778510102925
1116	11	0.028447199001654155
1116	12	0.025131597409703943
1116	13	-0.056880783658549205
1116	14	-0.11981174830940944
1116	15	-0.04304426725482102
1116	16	0.004655894026946328
1116	17	-0.1226268721201933
1116	18	-0.14052024883667258
1116	19	-0.15305972705967008
1116	20	-0.0931481322744645
1116	21	-0.058785947176374975
1116	22	-0.09368320258010954
1116	23	-0.1447006528153878
1116	24	-0.07130388812775834
1116	25	-0.17627263506253854
1201	1	0.3494591216473957
1201	2	0.37104991740654114
1201	3	0.2753763514527847
1201	4	0.37461791874714123
1201	5	0.4022367702822365
1201	6	0.1732150286410885
1201	7	0.29778785313168044
1201	8	0.23623702796183466
1201	9	0.2841307972840781
1201	10	0.3565396337526252
1201	11	0.3685438123666529
1201	12	0.3822370684351668
1201	13	0.3226937882108629
1201	14	0.3802847410421393
1201	15	0.4767554096901492
1201	16	0.3686182063403507
1201	17	0.4196171029792879
1201	18	0.41647099202465654
1201	19	0.4179296694461172
1201	20	0.4001616522831739
1201	21	0.5203218518570765
1201	22	0.4383628022750159
1201	23	0.5628628239842755
1201	24	0.45895088027540965
1201	25	0.5265075741825953
1202	1	0.47746852334932655
1202	2	0.48986491375965713
1202	3	0.43236478138452483
1202	4	0.50809669669313
1202	5	0.591155487141485
1202	6	0.4039430410074303
1202	7	0.40028419199431653
1202	8	0.4742580487625645
1202	9	0.5610023292757447
1202	10	0.5902532642059839
1202	11	0.6067868009037696
1202	12	0.5982005775474377
1202	13	0.662387814808227
1202	14	0.810159165835934
1202	15	0.7784830712410482
1202	16	0.8427258599323295
1202	17	0.8135288776455454
1202	18	0.7700063033287066
1202	19	0.7685553790112181
1202	20	0.8198820413500485
1202	21	0.785631753404509
1202	22	0.9235467979451286
1202	23	0.8454363422451152
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2210	13	-0.43239363278471643
2210	14	-0.5402014918028542
2210	15	-0.3971087512290836
2210	16	-0.50660786206587
2210	17	-0.5355498909438765
2210	18	-0.4724499281707182
2210	19	-0.48086581416354335
2210	20	-0.41553455532552874
2210	21	-0.4089648889367936
2210	22	-0.4588231738380628
2210	23	-0.4873277821845221
2210	24	-0.4704518894497767
2210	25	-0.4643289335327907
2211	1	-0.712530061713494
2211	2	-0.5145049779306028
2211	3	-0.4132207281932665
2211	4	-1.213822131050108
2211	5	-0.7652050511618782
2211	6	-0.5887949826045045
2211	7	-0.662684469308779
2211	8	-0.4752144786310524
2211	9	-0.5725341805366
2211	10	-0.422665310079573
2211	11	-0.4587464798328824
2211	12	-0.4892456909276248
2211	13	-0.5229699082024695
2211	14	-0.6670719596458383
2211	15	-0.5781625061936424
2211	16	-0.43906213997885857
2211	17	-0.45888118829521574
2211	18	-0.48734138630457124
2211	19	-0.5589392684448384
2211	20	-0.4830864909893293
2211	21	-0.5286918146640502
2211	22	-0.4617929873845057
2211	23	-0.5057888021044903
2211	24	-0.6379661915501558
2211	25	-0.6564924947573374
2212	1	-0.22970013975712789
2212	2	-0.45282494773279325
2212	3	-0.4939943923589247
2212	4	-0.16053491614380277
2212	5	-0.30535959772242194
2212	6	-0.2890145885436084
2212	7	-0.2817360045620063
2212	8	-0.33599605591151516
2212	9	-0.44973536693473193
2212	10	-0.30383381105232843
2212	11	-0.4437673601984855
2212	12	-0.4472640559887182
2212	13	-0.455728072878955
2212	14	-0.42956691852255346
2212	15	-0.3700966534215837
2212	16	-0.43346209484037956
2212	17	-0.3527168496541577
2212	18	-0.42546564497056494
2212	19	-0.4143473258167987
2212	20	-0.5135072886704393
2212	21	-0.5872271416692527
2212	22	-0.572233961356936
2212	23	-0.5727666355974819
2212	24	-0.5867413000917594
2212	25	-0.7033201317514894
2213	1	-0.22960309230301235
2213	2	-0.2893473326945184
2213	3	-0.30996884779878897
2213	4	-0.03476808299012646
2213	5	-0.18837545814542267
2213	6	-0.2615535937405298
2213	7	-0.4828107192485547
2213	8	-0.47073859999334644
2213	9	-0.4999421029304969
2213	10	-0.3787314802028092
2213	11	-0.40510752021296526
2213	12	-0.4179520207439893
2213	13	-0.46562522951651175
2213	14	-0.5736943083582062
2213	15	-0.5766583543880017
2213	16	-0.4918375191115061
2213	17	-0.5518549254853298
2213	18	-0.5327138573376047
2213	19	-0.4736285712245518
2213	20	-0.5264336593529251
2213	21	-0.5443701508245766
2213	22	-0.5615584046675508
2213	23	-0.5757119349110909
2213	24	-0.590493285321692
2213	25	-0.6371500660892195
2214	1	-0.31794426196609393
2214	2	-0.39238086066065847
2214	3	-0.39956509904878246
2214	4	-0.17119714325703939
2214	5	-0.3684277232531201
2214	6	-0.3760949880388935
2214	7	-0.38502498134226215
2214	8	-0.464843179375805
2214	9	-0.5259375970078537
2214	10	-0.5238323136372571
2214	11	-0.47750389337610954
2214	12	-0.6024369865953574
2214	13	-0.5385245076331273
2214	14	-0.6148411854175677
2214	15	-0.5579241205574164
2214	16	-0.668651631430734
2214	17	-0.6333935952415857
2214	18	-0.6554998529744225
2214	19	-0.6257407323782971
2214	20	-0.7014648050959664
2214	21	-0.7649815352181619
2214	22	-0.8793398814291109
2214	23	-0.6991603640897779
2214	24	-0.7898006642656625
2214	25	-0.7196727377846699
2215	1	-0.34772298136345725
2215	2	-0.3727586339998261
2215	3	-0.32140710669587236
2215	4	-0.13451440247825275
2215	5	-0.3738257873847104
2215	6	-0.3798036059188874
2215	7	-0.4882262501508805
2215	8	-0.505206400412284
2215	9	-0.6185266228595907
2215	10	-0.6572823190269972
2215	11	-0.6406985359728239
2215	12	-0.719095568649422
2215	13	-0.6787727210922654
2215	14	-0.7966383630951626
2215	15	-0.953654811015042
2215	16	-0.8205099826770379
2215	17	-0.8395678614441024
2215	18	-0.8973054640126108
2215	19	-0.8552723955257804
2215	20	-0.851444672825366
2215	21	-0.7986178830023434
2215	22	-0.9528547697980514
2215	23	-0.9201504754989145
2215	24	-0.9667859122254825
2215	25	-0.9984761517104985
2216	1	-0.3609773702803878
2216	2	-0.28872120953739255
2216	3	-0.30768064045173915
2216	4	-0.17255713560344077
2216	5	-0.3968998001770174
2216	6	-0.41040008522953997
2216	7	-0.5795073870003264
2216	8	-0.5940123771501433
2216	9	-0.7538924594180045
2216	10	-0.6016530176183466
2216	11	-0.640129991799057
2216	12	-0.6809889570740211
2216	13	-0.7246517301141893
2216	14	-0.9027317700081596
2216	15	-0.7530754386649576
2216	16	-0.7773964431461451
2216	17	-0.9473269119298919
2216	18	-0.8873888539536807
2216	19	-0.8796318972115458
2216	20	-0.8771219921324445
2216	21	-1.0361866432202973
2216	22	-0.8870733390038552
2216	23	-0.9542398855944043
2216	24	-0.8825119297624582
2216	25	-1.0951917606954424
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53047.0
3	1.0
4	836.0
5	7108.0
6	8147.0
7	6330.0
8	5090.0
9	4533.0
10	4109.0
11	3696.0
12	3425.0
13	3078.0
14	3262.0
15	3199.0
16	3377.0
17	3588.0
18	3899.0
19	4698.0
20	6021.0
21	7995.0
22	10013.0
23	12268.0
24	14314.0
25	16049.0
26	17947.0
27	19383.0
28	22711.0
29	27271.0
30	34540.0
31	43977.0
32	56390.0
33	73389.0
34	97964.0
35	131913.0
36	187385.0
37	305679.0
38	1181814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.51506070340612	19.656896344875552	13.311374788895106	27.516668162823223
2	13.486922543538485	20.849021368108293	40.358683745967724	25.305372342385496
3	16.616317361822038	25.33928732181475	30.312584395274495	27.731810921088716
4	10.948444241610963	17.709958821546778	38.51853867780948	32.82305825903278
5	12.333000612641877	38.485307909114454	36.52028632417388	12.661405154069794
6	31.164079673561577	38.23395705748301	18.273551832854924	12.328411436100486
7	27.17135462038464	31.841536554312132	23.15444280640622	17.832666018897005
8	24.61993351113389	37.41730243137866	20.804926490817476	17.157837566669976
9	26.184267382151077	14.74857556299177	20.107213797862254	38.9599432569949
10	13.962100936883465	29.372778795684372	35.52231169379285	21.142808573639307
11	34.00675585715566	22.273101422431154	25.215558568207108	18.50458415220608
12	22.588704119749828	25.06650767905157	32.41789849969394	19.92688970150466
13	28.673706669524872	21.953814321110883	26.04073945988312	23.331739549481124
14	20.527382348015408	22.067995310668227	28.10178362083403	29.30283872048233
15	22.71912364776093	31.002082525625447	23.472919211905985	22.805874614707637
16	21.931749765328586	28.25502439661604	27.77031592926949	22.04290990878588
17	21.49283870770512	28.766740078235877	27.949287807272228	21.79113340678678
18	21.39155461407084	27.75654411450598	29.620583335217976	21.231317936205198
19	23.183735366007856	27.209365420026245	27.621055698977493	21.985843514988407
20	23.299584750921728	27.491724744875956	28.088891270725817	21.119799233476495
21	22.86913524422003	26.87839659691344	27.287872317264135	22.964595841602396
22	22.926377498962715	28.189182818415865	27.244005608679466	21.640434073941954
23	22.075506063974775	27.77712877650699	27.76377191164371	22.38359324787452
24	22.709326482574863	27.29519528597255	28.196073932590483	21.799404298862104
25	22.50172078558258	27.490659190669742	27.776451949470033	22.231168074277647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	139.0
2	275.5
3	412.0
4	412.0
5	412.0
6	990.0
7	1568.0
8	1568.0
9	1568.0
10	2574.0
11	3580.0
12	3580.0
13	3580.0
14	6752.5
15	9925.0
16	9925.0
17	9925.0
18	19125.0
19	28325.0
20	28325.0
21	28325.0
22	45577.5
23	62830.0
24	62830.0
25	62830.0
26	89969.0
27	117108.0
28	117108.0
29	117108.0
30	151111.0
31	185114.0
32	185114.0
33	185114.0
34	211906.0
35	238698.0
36	238698.0
37	238698.0
38	258909.0
39	279120.0
40	279120.0
41	279120.0
42	290416.0
43	301712.0
44	301712.0
45	301712.0
46	309085.5
47	316459.0
48	316459.0
49	316459.0
50	302068.0
51	287677.0
52	287677.0
53	287677.0
54	254297.0
55	220917.0
56	220917.0
57	220917.0
58	187250.5
59	153584.0
60	153584.0
61	153584.0
62	124049.0
63	94514.0
64	94514.0
65	94514.0
66	72214.0
67	49914.0
68	49914.0
69	49914.0
70	36460.0
71	23006.0
72	23006.0
73	23006.0
74	15985.0
75	8964.0
76	8964.0
77	8964.0
78	6160.0
79	3356.0
80	3356.0
81	3356.0
82	2244.5
83	1133.0
84	1133.0
85	1133.0
86	723.5
87	314.0
88	314.0
89	314.0
90	190.5
91	67.0
92	67.0
93	67.0
94	38.5
95	10.0
96	10.0
97	10.0
98	5.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19091911644642584
2	0.008959800640248932
3	0.005484737775105654
4	0.0025539618647438546
5	0.08624854821921868
6	0.34892980624221775
7	0.03207106210481627
8	0.01578432168866284
9	0.005610342456978303
10	0.0042705591836700516
11	0.0
12	0.0
13	0.0
14	0.0019259384553806116
15	0.0
16	0.0
17	0.022566974509785863
18	0.03048006946776272
19	0.0
20	0.0
21	0.0010885739095629543
22	2.0934113645441427E-4
23	0.00674078459383214
24	0.0
25	8.373645458176572E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2388446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.76251604622726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0322159425793	60.41632944875733
2	11.81694104916104	17.196607254138037
3	2.8662411546449906	6.2566475402163935
4	0.9820867063592975	2.8583639892101944
5	0.4249894241065283	1.5461649895514074
6	0.23108212006947954	1.008846988173103
7	0.14705649987361633	0.7490140652229217
8	0.09611402174121833	0.5594798439370268
9	0.06615064956108861	0.4331958930141359
>10	0.3035870842543064	3.822145203526131
>50	0.0182669559135684	0.914107169376924
>100	0.012391851883434212	1.7867212604054907
>500	0.0018985163023968676	0.9457623067165019
>1k	9.780235497151152E-4	1.5066140477543317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3575	0.14967891256490623	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3466	0.14511527579019998	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3268	0.13682536678660517	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3187	0.13343404037604367	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3025	0.12665138755492064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.186822729088286E-5	0.0	0.0	0.0
6	0.0	4.186822729088286E-5	0.0	0.0	0.0
7	0.0	4.186822729088286E-5	0.0	0.0	0.0
8	0.0	4.186822729088286E-5	0.0	0.0	0.0
9	0.0	4.186822729088286E-5	0.0	0.0	0.0
10	0.0	4.186822729088286E-5	0.0	0.0	0.0
11	4.186822729088286E-5	4.186822729088286E-5	0.0	4.186822729088286E-5	0.0
12	4.186822729088286E-5	4.186822729088286E-5	0.0	4.186822729088286E-5	0.0
13	4.186822729088286E-5	4.186822729088286E-5	0.0	4.186822729088286E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGCGC	40	0.0053293235	14.230122	10
TAGGACC	680	0.0	12.1826	4
GGCGAGG	790	0.0	11.288029	19
TCGAACT	135	4.836693E-9	11.243552	19
AACCGCT	70	0.0015059595	10.84495	7
TATAGCG	80	3.6923675E-4	10.712287	5
AGGACGT	1930	0.0	10.508772	5
GGACGTG	1915	0.0	10.490537	6
AATCCCG	190	9.094947E-12	10.485353	19
GACGTGG	810	0.0	10.309397	7
ACGAAAT	140	9.8340024E-8	10.168631	12
GACGTGA	1140	0.0	10.155249	7
TCGTCAA	150	2.4998371E-8	10.119198	10
AGGACCT	1370	0.0	10.078064	5
GTAGGAC	1935	0.0	10.039154	3
TGGCGAG	1950	0.0	9.977216	18
TGTAGGA	2095	0.0	9.910243	2
CGTGGAA	815	0.0	9.8943615	9
TTAGGAC	1320	0.0	9.883131	3
GGACCTG	1330	0.0	9.878966	6
>>END_MODULE