FastQCFastQC Report
Thu 26 May 2016
SRR1513120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162737
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23570.1089822756997268No Hit
GTCCTACAGTGGACATTTCTAAATT22470.10389612791569201No Hit
GTATCAACGCAGAGTACTTTTTTTT22150.10241652128760918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG5750.012.21293119
TAGGACC5700.011.3682024
GTCCTAA7600.010.6817541
TGGCGAG12000.010.67618
GTCTTAT2550.010.4870931
TCTATAC2300.010.3578583
GTAGGAC12700.010.2795613
CGCAGAT1303.9075348E-710.2183438
TTAGGAC9350.010.1916893
TGTAGGA13500.010.1708892
ATAGGAC2250.010.1645113
TAGGACA6000.010.1645114
GTGCTAG1605.657057E-910.1476671
GACGTGA6300.010.0909017
AGGACGT10750.010.0857385
GGACGTG10350.010.0834226
CTCTTAT3250.09.9915491
CGTGCGC951.6640125E-49.98831510
TTTAGGA10250.09.5817212
TAGGACG11750.09.5697784
Produced by FastQC (version 0.11.5)