##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162737 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.599691039640973 31.0 30.0 34.0 25.0 34.0 2 29.470450637317438 31.0 30.0 34.0 23.0 34.0 3 29.666670519808928 31.0 30.0 34.0 25.0 34.0 4 32.74383385497173 37.0 35.0 37.0 25.0 37.0 5 32.91521530357135 37.0 35.0 37.0 26.0 37.0 6 33.25965154339155 37.0 35.0 37.0 27.0 37.0 7 33.23057403651022 37.0 35.0 37.0 27.0 37.0 8 33.10778934285584 37.0 35.0 37.0 26.0 37.0 9 34.37268516698979 39.0 35.0 39.0 25.0 39.0 10 33.979138933675245 39.0 34.0 39.0 22.0 39.0 11 34.408253523197686 39.0 35.0 39.0 27.0 39.0 12 34.40215939339827 39.0 35.0 39.0 27.0 39.0 13 34.44006922709511 39.0 35.0 39.0 27.0 39.0 14 34.69711296380466 40.0 34.0 41.0 17.0 41.0 15 35.12232971461625 40.0 35.0 41.0 24.0 41.0 16 34.96489170897802 40.0 35.0 41.0 22.0 41.0 17 34.58903509765635 39.0 34.0 41.0 17.0 41.0 18 34.33216752661096 39.0 33.0 41.0 17.0 41.0 19 34.95994150005294 40.0 34.0 41.0 22.0 41.0 20 35.1736595804298 40.0 35.0 41.0 25.0 41.0 21 35.220749448499745 40.0 36.0 41.0 25.0 41.0 22 34.918498643154486 40.0 35.0 41.0 19.0 41.0 23 34.45847923256503 40.0 34.0 41.0 16.0 41.0 24 34.70623150202729 40.0 35.0 41.0 18.0 41.0 25 34.35560495797686 40.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0363713006350146 1101 2 1.2163856569288036 1101 3 1.2075309953813793 1101 4 1.492080301768027 1101 5 1.3893040535318164 1101 6 1.1279775775151535 1101 7 1.039493262429012 1101 8 1.0746182654858814 1101 9 1.4062007138407537 1101 10 1.7674701792907612 1101 11 1.4677074347907038 1101 12 1.3073904026960221 1101 13 1.3074011425898533 1101 14 2.387284225999217 1101 15 1.9078608063632458 1101 16 1.973585946291358 1101 17 2.3037343271464366 1101 18 2.308331050854086 1101 19 1.8545608074989914 1101 20 1.4751079389493995 1101 21 1.5626398073789858 1101 22 1.7949614070316642 1101 23 1.9878918396374345 1101 24 1.76732552256329 1101 25 1.935566387754605 1102 1 1.0285856040677608 1102 2 1.1943070223952503 1102 3 1.0734367904087598 1102 4 1.4086553776166681 1102 5 1.3218498012372777 1102 6 1.0751997997373763 1102 7 1.1415311581299648 1102 8 1.060128311853731 1102 9 1.4429348333286072 1102 10 1.553959102526207 1102 11 1.2188112949620589 1102 12 1.1726393198254144 1102 13 1.072893824865119 1102 14 2.1007237474371934 1102 15 1.7201377922620793 1102 16 1.8440369356630057 1102 17 2.088153393301724 1102 18 2.3882034915585706 1102 19 1.789413021897829 1102 20 1.4916925555082585 1102 21 1.4250079070603263 1102 22 1.6864358283875873 1102 23 1.9930366676184263 1102 24 1.8437399328519888 1102 25 2.0690251814272003 1103 1 0.6318276201885666 1103 2 0.814909511937131 1103 3 0.5982327295986387 1103 4 1.0380554388349168 1103 5 0.9158019489773039 1103 6 0.5865634361010166 1103 7 0.5586416570586508 1103 8 0.5487340663685814 1103 9 0.8471308337367276 1103 10 1.1197304523884668 1103 11 0.7822742069098183 1103 12 0.6630688678307166 1103 13 0.5313546962047795 1103 14 1.4720965665372816 1103 15 1.0754688809265005 1103 16 1.0836782987892164 1103 17 1.4718744421742826 1103 18 1.7265847791822821 1103 19 1.0161449880237328 1103 20 0.8286734758225123 1103 21 0.8598130289866646 1103 22 1.0884458273672806 1103 23 1.4997018038290193 1103 24 1.1559172616959899 1103 25 1.408722661276201 1104 1 0.8252992112018163 1104 2 1.0245544961242707 1104 3 0.9415279154115623 1104 4 1.3234735533241349 1104 5 1.2068977499724625 1104 6 0.8913169604552635 1104 7 0.8849740565637916 1104 8 0.8588125223800489 1104 9 1.179783100228292 1104 10 1.3410885641112031 1104 11 0.9347934598250447 1104 12 0.7943753329104837 1104 13 0.8630536913256535 1104 14 1.7785502581584396 1104 15 1.3255376264580434 1104 16 1.4725905194948723 1104 17 1.8809734062971728 1104 18 1.9330652468407763 1104 19 1.3467207228231928 1104 20 1.134401659046155 1104 21 1.093260354910477 1104 22 1.4439496640623446 1104 23 1.724704678642155 1104 24 1.5210079752440535 1104 25 1.7487461153072417 1105 1 0.5683138315410865 1105 2 0.8967607959274524 1105 3 0.7120729001232995 1105 4 1.0345394064418727 1105 5 1.004438762747803 1105 6 0.7545235805317461 1105 7 0.7365202648837794 1105 8 0.8588839679623277 1105 9 1.0620971706281992 1105 10 1.2445699622547153 1105 11 0.8418323552380187 1105 12 0.8541487327073725 1105 13 0.6733560895430699 1105 14 1.5672856713520602 1105 15 1.3001500820782894 1105 16 1.4870638615330734 1105 17 1.7813465110046423 1105 18 1.419518781207195 1105 19 1.128690157253395 1105 20 0.9136438431458203 1105 21 0.7886314476446543 1105 22 1.0915025844347355 1105 23 1.4237011556358539 1105 24 1.2699870698834417 1105 25 1.4449189862425769 1106 1 0.4927627676950088 1106 2 0.7435302104484371 1106 3 0.6584694517154333 1106 4 1.1150823928672153 1106 5 0.9610029112498495 1106 6 0.5368757560759221 1106 7 0.5534208322173129 1106 8 0.6340085746187398 1106 9 0.6744070067063248 1106 10 0.559321399729356 1106 11 0.5531491662263761 1106 12 0.5636065884775618 1106 13 0.39198622023650387 1106 14 1.5006327860667454 1106 15 1.0595256323765057 1106 16 1.1912495733031392 1106 17 1.5132909873504303 1106 18 1.5307069659695784 1106 19 1.1012024265109588 1106 20 0.9413781074880276 1106 21 0.8401722826432376 1106 22 1.0784485537635078 1106 23 1.413051210378569 1106 24 1.0121404872832045 1106 25 1.1638936514698557 1107 1 0.5799529737266624 1107 2 0.7384540683164573 1107 3 0.7322228109271371 1107 4 0.9992269136196796 1107 5 0.9162134218500242 1107 6 0.6467593034034778 1107 7 0.6746510501928284 1107 8 0.750162650462272 1107 9 0.9738993924931236 1107 10 1.0970938691232064 1107 11 0.8069562728253601 1107 12 0.8547382695763943 1107 13 0.689985623043313 1107 14 1.5394503435465907 1107 15 0.9117203173570942 1107 16 1.2049850167190783 1107 17 1.5282208171503768 1107 18 1.6283480528611918 1107 19 1.1964547406345787 1107 20 1.0163258530837282 1107 21 0.9252723125028979 1107 22 1.1674303393711511 1107 23 1.375428039282717 1107 24 1.1599458023026088 1107 25 1.3561898592930817 1108 1 0.37625640692536066 1108 2 0.6631613751997563 1108 3 0.4160744260465954 1108 4 0.9186749116847537 1108 5 0.7825745780568454 1108 6 0.537087556538232 1108 7 0.3959501860881005 1108 8 0.4563000229715044 1108 9 0.6365808042420795 1108 10 0.9217919863540018 1108 11 0.47632617674025113 1108 12 0.30340251734517665 1108 13 0.44507100404209865 1108 14 1.3587252235912004 1108 15 0.8777318462101462 1108 16 0.9503101277443093 1108 17 1.2502619257678589 1108 18 1.4699878805824937 1108 19 0.8428113208060637 1108 20 0.6765993432269539 1108 21 0.5835813466471578 1108 22 0.896577635362874 1108 23 1.1513478031471251 1108 24 0.8454456491566518 1108 25 1.2096126648250944 1109 1 0.32375166214461615 1109 2 0.4322591176830244 1109 3 0.4905425111529773 1109 4 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93127.0 3 2.0 4 779.0 5 4597.0 6 5685.0 7 4983.0 8 4788.0 9 4471.0 10 4193.0 11 3909.0 12 3798.0 13 3940.0 14 3994.0 15 3913.0 16 3978.0 17 4289.0 18 4943.0 19 6069.0 20 7695.0 21 9617.0 22 12227.0 23 14463.0 24 16799.0 25 19133.0 26 21802.0 27 24729.0 28 27565.0 29 32493.0 30 39279.0 31 47162.0 32 60678.0 33 78822.0 34 104948.0 35 141093.0 36 202640.0 37 296506.0 38 843628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25984456198531 18.795031170972553 12.440830731892582 25.504293535149557 2 14.295355927222294 20.47087366714736 41.551488451584824 23.682281954045525 3 18.11065306082715 25.30688931217206 30.028402414042947 26.554055212957845 4 11.231466925500815 16.9888702864746 39.64592974789213 32.13373304013245 5 12.42910224295565 38.15620405204206 36.70232490872986 12.712368796272433 6 31.137009519977614 37.66276109411528 18.581275912861102 12.618953473045998 7 27.463361912482963 31.78547462677456 23.025816503638026 17.725346957104453 8 24.803372280428334 36.60741744456802 21.27089465785829 17.318315617145355 9 26.47010808363475 14.932893432801169 20.37509012365034 38.22190835991374 10 13.624551451242247 28.943633278467672 36.10903636996815 21.322778900321936 11 33.9782256912055 22.619160485285622 24.876646010933865 18.525967812575008 12 22.665784198642257 24.960293563996043 32.29518462914605 20.078737608215647 13 28.611474559895512 21.96056993086071 26.0593934668019 23.368562042441877 14 20.287515112048844 21.66986867895487 29.07613697644324 28.966479232553045 15 23.060448704961363 30.36228887451859 23.69008995007091 22.88717247044914 16 22.00922550694503 28.10301785256763 27.81188808407476 22.07586855641258 17 21.429248763203482 28.347260544861197 28.38334100428571 21.840149687649614 18 21.316535835135618 27.63604104958771 29.742172771624205 21.305250343652464 19 23.13567823391995 27.321987617052706 27.742630425864423 21.79970372316292 20 23.451592866039594 27.382800774614047 27.906271145441792 21.259335213904574 21 23.150398729320585 27.03038353963218 27.19476890073187 22.624448830315362 22 22.950968301796724 27.724941785324077 27.36853012222075 21.95555979065845 23 22.161813153903754 27.519586182208037 27.95874032369291 22.3598603401953 24 22.816198910025022 27.280044742543026 27.877537180211327 22.026219167220628 25 22.61776956504645 27.173831092691568 27.833511798349182 22.3748875439128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 355.5 3 486.0 4 486.0 5 486.0 6 1009.5 7 1533.0 8 1533.0 9 1533.0 10 2525.0 11 3517.0 12 3517.0 13 3517.0 14 6682.5 15 9848.0 16 9848.0 17 9848.0 18 18278.0 19 26708.0 20 26708.0 21 26708.0 22 42285.0 23 57862.0 24 57862.0 25 57862.0 26 81563.5 27 105265.0 28 105265.0 29 105265.0 30 133858.0 31 162451.0 32 162451.0 33 162451.0 34 187070.5 35 211690.0 36 211690.0 37 211690.0 38 231266.0 39 250842.0 40 250842.0 41 250842.0 42 263126.5 43 275411.0 44 275411.0 45 275411.0 46 281384.0 47 287357.0 48 287357.0 49 287357.0 50 274049.5 51 260742.0 52 260742.0 53 260742.0 54 231027.0 55 201312.0 56 201312.0 57 201312.0 58 170532.5 59 139753.0 60 139753.0 61 139753.0 62 113065.5 63 86378.0 64 86378.0 65 86378.0 66 66255.5 67 46133.0 68 46133.0 69 46133.0 70 33865.0 71 21597.0 72 21597.0 73 21597.0 74 15218.0 75 8839.0 76 8839.0 77 8839.0 78 6027.0 79 3215.0 80 3215.0 81 3215.0 82 2164.5 83 1114.0 84 1114.0 85 1114.0 86 718.5 87 323.0 88 323.0 89 323.0 90 212.5 91 102.0 92 102.0 93 102.0 94 62.0 95 22.0 96 22.0 97 22.0 98 17.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.170016049108144 2 0.25097827428855196 3 0.2555095695870557 4 0.4388883160550728 5 0.2499148070246174 6 0.020437066550394244 7 0.02025211572188389 8 0.018587558265290693 9 0.01988221406486318 10 0.022471525664008153 11 0.021454296107201197 12 0.02862114071197746 13 0.026817870134001498 14 0.02566192745581178 15 0.04027304290812984 16 0.02177796005709432 17 0.04170641182908509 18 0.0309330260683569 19 0.026355493062725614 20 0.02903728007612576 21 0.03440085410292606 22 0.04212255119323339 23 0.028944804661870584 24 0.04790226458418199 25 0.036157886973774436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2162737.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.946386107169 #Duplication Level Percentage of deduplicated Percentage of total 1 83.14944739138035 61.48601141400748 2 11.584692150404797 17.132922373730462 3 2.9038998424286944 6.441986968943383 4 0.9948696651610168 2.94268065545226 5 0.47725173543760835 1.7645520549492928 6 0.2539255540666441 1.126612623809333 7 0.1423926820664148 0.7370596972842929 8 0.09687734739405365 0.5730983788351227 9 0.06998836096978964 0.46578477269519836 >10 0.30168447157664696 3.6906105082068894 >50 0.01335843337205685 0.6789316476243428 >100 0.009720918558694832 1.4545610381724665 >500 0.001260964788879472 0.6541984246485033 >1k 6.304823944396629E-4 0.8509894416411102 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2357 0.1089822756997268 No Hit GTCCTACAGTGGACATTTCTAAATT 2247 0.10389612791569201 No Hit GTATCAACGCAGAGTACTTTTTTTT 2215 0.10241652128760918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.623770712758879E-5 2 0.0 0.0 0.0 0.0 4.623770712758879E-5 3 0.0 0.0 0.0 0.0 4.623770712758879E-5 4 0.0 0.0 0.0 0.0 4.623770712758879E-5 5 0.0 0.0 0.0 0.0 4.623770712758879E-5 6 0.0 0.0 0.0 0.0 9.247541425517758E-5 7 0.0 4.623770712758879E-5 0.0 0.0 9.247541425517758E-5 8 0.0 4.623770712758879E-5 0.0 0.0 9.247541425517758E-5 9 0.0 4.623770712758879E-5 0.0 4.623770712758879E-5 9.247541425517758E-5 10 0.0 4.623770712758879E-5 0.0 4.623770712758879E-5 9.247541425517758E-5 11 0.0 4.623770712758879E-5 0.0 1.3871312138276639E-4 9.247541425517758E-5 12 0.0 4.623770712758879E-5 0.0 1.3871312138276639E-4 9.247541425517758E-5 13 0.0 4.623770712758879E-5 0.0 1.3871312138276639E-4 1.3871312138276639E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 575 0.0 12.212931 19 TAGGACC 570 0.0 11.368202 4 GTCCTAA 760 0.0 10.681754 1 TGGCGAG 1200 0.0 10.676 18 GTCTTAT 255 0.0 10.487093 1 TCTATAC 230 0.0 10.357858 3 GTAGGAC 1270 0.0 10.279561 3 CGCAGAT 130 3.9075348E-7 10.218343 8 TTAGGAC 935 0.0 10.191689 3 TGTAGGA 1350 0.0 10.170889 2 ATAGGAC 225 0.0 10.164511 3 TAGGACA 600 0.0 10.164511 4 GTGCTAG 160 5.657057E-9 10.147667 1 GACGTGA 630 0.0 10.090901 7 AGGACGT 1075 0.0 10.085738 5 GGACGTG 1035 0.0 10.083422 6 CTCTTAT 325 0.0 9.991549 1 CGTGCGC 95 1.6640125E-4 9.988315 10 TTTAGGA 1025 0.0 9.581721 2 TAGGACG 1175 0.0 9.569778 4 >>END_MODULE