FastQCFastQC Report
Thu 26 May 2016
SRR1513120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162737
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31680.1464810561802013No Hit
CCCATGTACTCTGCGTTGATACCAC25310.11702763673992723No Hit
GTACATGGGAAGCAGTGGTATCAAC24700.11420713660514432No Hit
TATCAACGCAGAGTACTTTTTTTTT23650.10935217735674749No Hit
GTCCTACAGTGGACATTTCTAAATT22660.1047746443511162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTG12350.010.7152576
AGGACGT12900.010.7022075
TAGGACC5900.010.6509164
GTCCTAG2900.010.5108651
GGCGAGG6800.010.465713519
TAGGACG13550.010.399624
GTAAGAC2151.8189894E-1210.1857853
GTAGGAC13500.010.08573153
TGTAGGA13600.09.9422522
TTAGGAC9800.09.9101283
TGGCGAG13500.09.841948518
GACGTGA7050.09.8283547
GTCCTAA6750.09.7371491
ACGTGGA6650.09.5611438
TCTACAC3000.09.5214953
ACCGTGC1002.785873E-49.4897928
GACGTGG6550.09.419317
CTGTAGG14700.09.3958741
GTCTTAG3150.09.3742741
TAAAGGT3150.09.3701424
Produced by FastQC (version 0.11.5)