##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513120_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2162737
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.748867291769642	31.0	31.0	34.0	27.0	34.0
2	30.862029456193703	33.0	31.0	34.0	27.0	34.0
3	30.952198071240286	33.0	31.0	34.0	27.0	34.0
4	34.34306390467265	37.0	35.0	37.0	31.0	37.0
5	34.08891002465857	37.0	35.0	37.0	30.0	37.0
6	33.99961900129327	37.0	35.0	37.0	30.0	37.0
7	33.86812820976383	37.0	35.0	37.0	29.0	37.0
8	33.8376552488814	37.0	35.0	37.0	29.0	37.0
9	35.39055465366339	39.0	35.0	39.0	29.0	39.0
10	35.23764840570074	39.0	35.0	39.0	29.0	39.0
11	35.24207474140406	39.0	35.0	39.0	29.0	39.0
12	35.18176412573512	39.0	35.0	39.0	28.0	39.0
13	35.14667201791064	39.0	35.0	39.0	28.0	39.0
14	36.312369002796	40.0	37.0	41.0	28.0	41.0
15	36.28980500171773	40.0	37.0	41.0	27.0	41.0
16	36.25932325567094	40.0	37.0	41.0	27.0	41.0
17	36.19438193363317	40.0	36.0	41.0	27.0	41.0
18	36.1531952336322	40.0	36.0	41.0	27.0	41.0
19	36.231020230384	40.0	37.0	41.0	27.0	41.0
20	36.1977586733847	40.0	37.0	41.0	27.0	41.0
21	36.14537088883207	40.0	37.0	41.0	27.0	41.0
22	36.08824882544665	40.0	37.0	41.0	27.0	41.0
23	36.009348802004126	40.0	37.0	41.0	27.0	41.0
24	35.945487130427786	40.0	37.0	41.0	27.0	41.0
25	35.8906922108421	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4841615553253149
1101	2	0.6479211519967443
1101	3	0.51859902912026
1101	4	0.6948145518636935
1101	5	0.686902761955082
1101	6	0.6144241301555198
1101	7	0.6752177130662389
1101	8	0.6364839822738801
1101	9	0.7828359201196164
1101	10	0.8684219898827195
1101	11	0.8239902356467539
1101	12	0.7557085756472759
1101	13	0.7439013957754881
1101	14	0.9109369432088883
1101	15	1.0436370995124662
1101	16	0.9763392226824052
1101	17	0.8962828169888652
1101	18	0.9411034978434074
1101	19	0.9476350506525222
1101	20	0.9026206382159572
1101	21	0.9556480499123552
1101	22	1.0002170516444906
1101	23	1.0072369245247188
1101	24	0.9260744990782754
1101	25	0.9943866454063155
1102	1	0.5495340246519334
1102	2	0.6486739140323508
1102	3	0.5919346522240438
1102	4	0.7194391249169101
1102	5	0.7813829840628159
1102	6	0.7026900106676592
1102	7	0.7120472396562008
1102	8	0.6940960379153012
1102	9	0.7745450429467198
1102	10	0.8062323014823178
1102	11	0.8797666586436819
1102	12	0.8737171432050488
1102	13	0.927729414933232
1102	14	0.9973199645609938
1102	15	0.9443459742811058
1102	16	0.9624064465265505
1102	17	1.0693031196201872
1102	18	1.0212211348003066
1102	19	1.0970506821521298
1102	20	0.9895221971680002
1102	21	1.0172347654274603
1102	22	0.9815649631830254
1102	23	1.0176944969864081
1102	24	1.0302805211745607
1102	25	1.1010470152042444
1103	1	0.32112620912290524
1103	2	0.35058698412724
1103	3	0.33546082302294167
1103	4	0.45227250926544826
1103	5	0.4169737401081193
1103	6	0.21365317769143388
1103	7	0.3216508515325387
1103	8	0.22939600730617826
1103	9	0.4101638573534103
1103	10	0.33454828964678995
1103	11	0.3419516397680624
1103	12	0.3600017060047591
1103	13	0.43195092294261883
1103	14	0.4707282902420502
1103	15	0.4030185587112669
1103	16	0.46476589964358084
1103	17	0.5128573487804786
1103	18	0.478391321515943
1103	19	0.34778275030843986
1103	20	0.4888436949743493
1103	21	0.38694959044531174
1103	22	0.45422081462675834
1103	23	0.32564776706089305
1103	24	0.3301198232865943
1103	25	0.4512918881763923
1104	1	0.2960358955488438
1104	2	0.3891894749653346
1104	3	0.35314995100740276
1104	4	0.4869885860537906
1104	5	0.4557612189219995
1104	6	0.4163408363269596
1104	7	0.4157055713090898
1104	8	0.47671854245250955
1104	9	0.44458828643218595
1104	10	0.5964851526395876
1104	11	0.6279713818181563
1104	12	0.5796463179938698
1104	13	0.6366901148951385
1104	14	0.7890706535439875
1104	15	0.7553170481983358
1104	16	0.7043431049030175
1104	17	0.7054534411455862
1104	18	0.6558803402852789
1104	19	0.7021161075291147
1104	20	0.6874866165177806
1104	21	0.7522244586899873
1104	22	0.5869849627349097
1104	23	0.7042520466716056
1104	24	0.7064467509251884
1104	25	0.5924599344756061
1105	1	0.30434520838468515
1105	2	0.2750774669064917
1105	3	0.19815218197098616
1105	4	0.377028086427444
1105	5	0.34447582803801424
1105	6	0.24027729629416683
1105	7	0.26050961605975687
1105	8	0.29820614349599595
1105	9	0.295415385385688
1105	10	0.32938324277051123
1105	11	0.28023450960173335
1105	12	0.23129353558535826
1105	13	0.32750456715936593
1105	14	0.4023685651978326
1105	15	0.4839834247028705
1105	16	0.4242200323597416
1105	17	0.36426848468359196
1105	18	0.4235464238903006
1105	19	0.431709360438262
1105	20	0.4000495420090715
1105	21	0.5154234141063014
1105	22	0.47897292921844326
1105	23	0.40576499584122416
1105	24	0.4950157314980075
1105	25	0.4744088385660703
1106	1	0.3028522254046919
1106	2	0.31516750526467163
1106	3	0.3515190499989487
1106	4	0.3872799028900715
1106	5	0.35857877495672597
1106	6	0.3293172699564124
1106	7	0.3449910150230906
1106	8	0.35542031381855566
1106	9	0.35713759867553563
1106	10	0.3428093707382942
1106	11	0.2911493636339344
1106	12	0.3236180718035939
1106	13	0.4064106317327898
1106	14	0.4425841672527895
1106	15	0.39692643869096855
1106	16	0.39675067679245046
1106	17	0.32374050465128335
1106	18	0.48378790431691243
1106	19	0.4228908076364277
1106	20	0.4135170141209201
1106	21	0.5180648455324501
1106	22	0.42486110096508156
1106	23	0.28144468591327154
1106	24	0.36002710113762504
1106	25	0.4820590364820134
1107	1	0.35958557864446306
1107	2	0.4793956330640121
1107	3	0.34853308403895866
1107	4	0.48103923403369464
1107	5	0.500710754652161
1107	6	0.4356176581319886
1107	7	0.5256419556023673
1107	8	0.496656503725994
1107	9	0.5568987213987313
1107	10	0.6375242109891133
1107	11	0.6557358073484778
1107	12	0.5989241333144975
1107	13	0.683209676223683
1107	14	0.569786317148278
1107	15	0.5056155996345382
1107	16	0.626371459115532
1107	17	0.630697805338059
1107	18	0.6727995495841554
1107	19	0.6993912555313244
1107	20	0.7237588773636787
1107	21	0.8094951232272791
1107	22	0.7804896467996585
1107	23	0.6645441185797338
1107	24	0.6592208903284273
1107	25	0.6901808638931826
1108	1	0.1517248554787649
1108	2	0.1770654611083451
1108	3	0.23846458041414564
1108	4	0.2739532334715733
1108	5	0.16780319960350454
1108	6	0.020603823991521608
1108	7	0.09520104824453313
1108	8	0.15231449760963756
1108	9	0.18002087138728484
1108	10	0.30411583330101877
1108	11	0.1749637011561802
1108	12	0.21033676902405318
1108	13	0.13176377343517487
1108	14	0.2043658252253735
1108	15	0.365858791663463
1108	16	0.3346548436600756
1108	17	0.41038228439079205
1108	18	0.2561934985335341
1108	19	0.3853861982695861
1108	20	0.29759979329374886
1108	21	0.2153530390301981
1108	22	0.15579738159684808
1108	23	0.19800818896226957
1108	24	0.2127994725502731
1108	25	0.16604496190731766
1109	1	0.13724703108559666
1109	2	0.17297620928535196
1109	3	0.17036449051303748
1109	4	0.271256200208164
1109	5	0.20879560795431473
1109	6	0.19381329357498345
1109	7	0.1513143236415928
1109	8	0.1641077750045028
1109	9	0.1725540848524716
1109	10	0.35547808482434107
1109	11	0.2908799432898306
1109	12	0.30912185542900517
1109	13	0.23857752830481616
1109	14	0.3086136526152501
1109	15	0.3089151796366991
1109	16	0.28780637697342826
1109	17	0.312265213169141
1109	18	0.26050125999591955
1109	19	0.23698069432392543
1109	20	0.20274163726537608
1109	21	0.3245204548727756
1109	22	0.3878421323709986
1109	23	0.43034012383391484
1109	24	0.3743467705225001
1109	25	0.4022663683602161
1110	1	0.23376043228077137
1110	2	0.31418786962275647
1110	3	0.32235272098377976
1110	4	0.23313153927579577
1110	5	0.23087469129489335
1110	6	0.17507812454847738
1110	7	0.2895307140155907
1110	8	0.30405634370771395
1110	9	0.33101790314397306
1110	10	0.3319855294726324
1110	11	0.30019084397770257
1110	12	0.30982371679814236
1110	13	0.3396121763577966
1110	14	0.31900775791153535
1110	15	0.29281447120104076
1110	16	0.34831191732126854
1110	17	0.36445332961652355
1110	18	0.41099293394434966
1110	19	0.42994632649784137
1110	20	0.4446455995079077
1110	21	0.4333780747663951
1110	22	0.4823200061805508
1110	23	0.46242662007293944
1110	24	0.5002683787763615
1110	25	0.5090125373273651
1111	1	0.038383001383685666
1111	2	-0.01935048836887887
1111	3	0.0886492134792718
1111	4	0.17991705945375003
1111	5	0.07334005230718788
1111	6	-0.1074830744517854
1111	7	-0.04064168390750211
1111	8	0.02047111105748911
1111	9	-0.03447402097774699
1111	10	-0.04383722874617746
1111	11	0.014332934496287919
1111	12	0.06721471235601939
1111	13	0.026540899520995254
1111	14	-0.03989857346210357
1111	15	0.05172990394902399
1111	16	0.06211911511478263
1111	17	0.08230960670020693
1111	18	0.10104201770779042
1111	19	0.03871265327089901
1111	20	0.11880977419304628
1111	21	0.0297261870226464
1111	22	0.0810130557069968
1111	23	0.06244487443622404
1111	24	0.08024855532925557
1111	25	0.18140048160218214
1112	1	-0.3185272908688219
1112	2	-0.20618215134924256
1112	3	-0.15490077681502612
1112	4	-1.0435949851362096
1112	5	-0.3917944707882697
1112	6	-0.3362545425249266
1112	7	-0.17755302797482386
1112	8	-0.13487529681147947
1112	9	-0.0656751812587757
1112	10	-0.05193532917212451
1112	11	-0.007447232364874878
1112	12	-0.004539587199090533
1112	13	-0.08955831422122174
1112	14	-0.03457319315329954
1112	15	-0.07067583064316096
1112	16	-0.14968109388536277
1112	17	-0.10355122422300411
1112	18	-0.08802457702080346
1112	19	-0.17818414010916683
1112	20	-0.06304315309317587
1112	21	-0.05882377793724203
1112	22	8.53934646777077E-4
1112	23	-0.1825744242688785
1112	24	-0.22850678957601644
1112	25	-0.14200784915548326
1113	1	-0.6167714652351179
1113	2	-0.4718540607509425
1113	3	-0.5465011239776878
1113	4	-1.5068545446391681
1113	5	-0.828683997812675
1113	6	-0.6107391986835324
1113	7	-0.42662494316491717
1113	8	-0.40389450581992037
1113	9	-0.38787943827208693
1113	10	-0.2934393559907562
1113	11	-0.4107836434314436
1113	12	-0.411737282852215
1113	13	-0.3666296604542154
1113	14	-0.2611512726336471
1113	15	-0.29051086837715445
1113	16	-0.35079948990633625
1113	17	-0.4034641511967294
1113	18	-0.44231314415950607
1113	19	-0.46655583216359986
1113	20	-0.46797057929640573
1113	21	-0.4408293270337964
1113	22	-0.5245750420704525
1113	23	-0.5254168767987224
1113	24	-0.4499007239002992
1113	25	-0.44465826454359103
1114	1	-0.46302103459492017
1114	2	-0.5237628887177834
1114	3	-0.3240910098554153
1114	4	-1.1802681989208637
1114	5	-0.5560676131498212
1114	6	-0.42144610199441246
1114	7	-0.40846572823335237
1114	8	-0.3272090167698849
1114	9	-0.32935098037979316
1114	10	-0.2819297323615544
1114	11	-0.3100381205171914
1114	12	-0.3361348582692827
1114	13	-0.262352713209296
1114	14	-0.29926782186569056
1114	15	-0.328936377930205
1114	16	-0.48946301333630515
1114	17	-0.5442317843842162
1114	18	-0.4675273461666194
1114	19	-0.4503676914880401
1114	20	-0.4591863054029517
1114	21	-0.4613154840041602
1114	22	-0.3881394046984994
1114	23	-0.3689017011429456
1114	24	-0.36255265341296905
1114	25	-0.3528920702009941
1115	1	-0.311571746632616
1115	2	-0.30979077903441876
1115	3	-0.20097832996229315
1115	4	-0.007631659823999826
1115	5	-0.24735275860025752
1115	6	-0.3107962552984205
1115	7	-0.275627372672389
1115	8	-0.2963018859601334
1115	9	-0.27200542909395153
1115	10	-0.3541985157247396
1115	11	-0.3043986196680777
1115	12	-0.23240977379268912
1115	13	-0.3365225821619049
1115	14	-0.27682139132776484
1115	15	-0.2973004010446232
1115	16	-0.3054445936716661
1115	17	-0.29901782531551646
1115	18	-0.4379388036848937
1115	19	-0.43518798673590453
1115	20	-0.3299211793111283
1115	21	-0.375779673463569
1115	22	-0.4727261214425411
1115	23	-0.5041453936539426
1115	24	-0.45015328633537166
1115	25	-0.5459797693657933
1116	1	-0.3505868115931179
1116	2	-0.3728385772324998
1116	3	-0.3128063505712042
1116	4	-0.2370234304057064
1116	5	-0.550113783931593
1116	6	-0.5912900149554474
1116	7	-0.578293867960852
1116	8	-0.7000077356696011
1116	9	-0.7115864501816347
1116	10	-0.688632886032245
1116	11	-0.6412013415892517
1116	12	-0.7183717223901098
1116	13	-0.7375469069861751
1116	14	-0.7447638055298498
1116	15	-0.8300309183347778
1116	16	-0.8207907640488017
1116	17	-0.7116200401748287
1116	18	-0.7670781314470787
1116	19	-0.773783379110391
1116	20	-0.8894734061397074
1116	21	-0.8758419000745192
1116	22	-0.8879282525169501
1116	23	-1.0480957429390472
1116	24	-0.929555258000839
1116	25	-1.0114508010081948
1201	1	0.43017469028444566
1201	2	0.45182223202322547
1201	3	0.41152884133826007
1201	4	0.48950793009220916
1201	5	0.4077938109704391
1201	6	0.34547471425436527
1201	7	0.3361040463865024
1201	8	0.33296029960897755
1201	9	0.3929827918134876
1201	10	0.4686784707739946
1201	11	0.5069472505267143
1201	12	0.43608273155911803
1201	13	0.41383109033736076
1201	14	0.5477278701737305
1201	15	0.6057699734356632
1201	16	0.5094410868940145
1201	17	0.5349308205913132
1201	18	0.5525324904849427
1201	19	0.413721217458658
1201	20	0.5546657520139959
1201	21	0.516545298318448
1201	22	0.5263204085295285
1201	23	0.6251379487944817
1201	24	0.49853493425472095
1201	25	0.6064848131366958
1202	1	0.4256553363240734
1202	2	0.4367437578737636
1202	3	0.44773772139284773
1202	4	0.47201176641219433
1202	5	0.500001633088516
1202	6	0.4207997567036941
1202	7	0.5046634585482366
1202	8	0.538476353832678
1202	9	0.6926682897252903
1202	10	0.596964416748655
1202	11	0.6747672586766882
1202	12	0.6457342291447716
1202	13	0.6278235451103882
1202	14	0.6904762131546676
1202	15	0.7655638912665381
1202	16	0.7229914036991971
1202	17	0.7283842440820365
1202	18	0.7813905691191891
1202	19	0.681175684027238
1202	20	0.6729717868954381
1202	21	0.68978138049129
1202	22	0.5986576532809806
1202	23	0.771714718098572
1202	24	0.8386363557454644
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2210	19	-0.32740405330012834
2210	20	-0.32071391350181244
2210	21	-0.4018165731096275
2210	22	-0.3294017560860425
2210	23	-0.28620178801272544
2210	24	-0.3138792877621981
2210	25	-0.399273365227657
2211	1	-0.6436588401288574
2211	2	-0.5802412215756974
2211	3	-0.40086208151756964
2211	4	-1.4534489862914057
2211	5	-0.7075861031346165
2211	6	-0.6384541518675775
2211	7	-0.5536205935313205
2211	8	-0.5176931134407781
2211	9	-0.47072568710091645
2211	10	-0.3938090127978455
2211	11	-0.47494073904271517
2211	12	-0.5200298189802339
2211	13	-0.5365337327288628
2211	14	-0.6112029284107194
2211	15	-0.5667699973335516
2211	16	-0.6245115081735548
2211	17	-0.5166864789022156
2211	18	-0.5436592968124074
2211	19	-0.5261655130456901
2211	20	-0.5855774431547331
2211	21	-0.6144338454406295
2211	22	-0.5567358047765296
2211	23	-0.5971835043745344
2211	24	-0.6010915989770851
2211	25	-0.5796908092343642
2212	1	-0.29033603760768756
2212	2	-0.40215479763973505
2212	3	-0.40541347042528386
2212	4	-0.1657311859562114
2212	5	-0.3001829622621628
2212	6	-0.25864846492783755
2212	7	-0.42149805262403106
2212	8	-0.37049088118968854
2212	9	-0.42436275756750774
2212	10	-0.4906796850883026
2212	11	-0.4313959813342194
2212	12	-0.5043437777273638
2212	13	-0.4510900959143953
2212	14	-0.44539534110612067
2212	15	-0.5348204267728462
2212	16	-0.514865073415649
2212	17	-0.39711758967200694
2212	18	-0.4323981040598994
2212	19	-0.4610988471821358
2212	20	-0.5394434006730933
2212	21	-0.5592813519521087
2212	22	-0.5292507865885483
2212	23	-0.6292796429596166
2212	24	-0.6334490000850792
2212	25	-0.664507265410279
2213	1	-0.23129951308006014
2213	2	-0.30742178719723867
2213	3	-0.4246451005779939
2213	4	-0.13596570884477188
2213	5	-0.4066423649256734
2213	6	-0.35611512809236956
2213	7	-0.4809018064241428
2213	8	-0.4277942586582668
2213	9	-0.5739312094838525
2213	10	-0.5642133409160053
2213	11	-0.49951593761031177
2213	12	-0.6518487744738408
2213	13	-0.5930717033940383
2213	14	-0.5731589148366893
2213	15	-0.5719904552475654
2213	16	-0.5555837458925694
2213	17	-0.6011882014350078
2213	18	-0.5276375208608286
2213	19	-0.5538114358445512
2213	20	-0.5735941536582985
2213	21	-0.5614410334158251
2213	22	-0.6673901153238333
2213	23	-0.67476461439486
2213	24	-0.6518496150082669
2213	25	-0.6642402271201391
2214	1	-0.5804650683621411
2214	2	-0.6591608438024075
2214	3	-0.7058117545111244
2214	4	-0.4691766011520002
2214	5	-0.5188963142501848
2214	6	-0.6046699133589257
2214	7	-0.6158033637561999
2214	8	-0.6983773127041175
2214	9	-0.8099924475832836
2214	10	-0.7827864998023983
2214	11	-0.7805684459798101
2214	12	-0.7575688027812717
2214	13	-0.7273024896305103
2214	14	-0.965126816706011
2214	15	-0.8870437415655417
2214	16	-0.9049375252181733
2214	17	-0.8376702683457111
2214	18	-0.7128204478367834
2214	19	-0.8795520153307166
2214	20	-0.8872723011620778
2214	21	-0.965892361988061
2214	22	-0.9212541624825406
2214	23	-0.8240924786956967
2214	24	-0.9806905406391948
2214	25	-1.0380659525604088
2215	1	-0.44135563386511123
2215	2	-0.49565824904447453
2215	3	-0.4720151216317916
2215	4	-0.255948588444042
2215	5	-0.5359708129451164
2215	6	-0.5039451328732625
2215	7	-0.6007805112373035
2215	8	-0.7062242011965694
2215	9	-0.7904495274848031
2215	10	-0.8462091593351744
2215	11	-0.8318059657883197
2215	12	-0.8001310502773649
2215	13	-0.8981382617386018
2215	14	-1.031624116155072
2215	15	-1.0453752727273624
2215	16	-1.0112799141865665
2215	17	-0.9315084163678335
2215	18	-1.0894368972710495
2215	19	-1.0963929821213085
2215	20	-1.0533729752603307
2215	21	-1.0601568804170753
2215	22	-1.1190718250427167
2215	23	-1.0065777826943503
2215	24	-1.2037605010603087
2215	25	-1.2406757921462344
2216	1	-0.6945405206312643
2216	2	-0.6225587690323913
2216	3	-0.6672963344878582
2216	4	-0.47936400391166245
2216	5	-0.9061417544161188
2216	6	-0.8744987557068526
2216	7	-1.0252089096228474
2216	8	-0.975376679076497
2216	9	-1.1493358353870846
2216	10	-1.0885679058497004
2216	11	-1.0607078489427835
2216	12	-1.1292208456744177
2216	13	-1.1033114860186117
2216	14	-1.4559243102481645
2216	15	-1.4146258474133617
2216	16	-1.4579238710683953
2216	17	-1.5481669586005253
2216	18	-1.4593202844815707
2216	19	-1.5151748385339516
2216	20	-1.5238742781877619
2216	21	-1.6990667141691844
2216	22	-1.5794094972825263
2216	23	-1.5180938358757174
2216	24	-1.5051096399789543
2216	25	-1.5801093319826336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53211.0
3	1.0
4	885.0
5	7331.0
6	8186.0
7	6315.0
8	5159.0
9	4438.0
10	4038.0
11	3654.0
12	3384.0
13	3302.0
14	3133.0
15	3047.0
16	3159.0
17	3309.0
18	3630.0
19	4527.0
20	5602.0
21	7440.0
22	9286.0
23	11214.0
24	12927.0
25	14565.0
26	15951.0
27	17789.0
28	19980.0
29	24137.0
30	30230.0
31	39125.0
32	49797.0
33	65082.0
34	86111.0
35	116352.0
36	168091.0
37	272486.0
38	1075863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49520857643278	19.54948064924519	13.455036310314334	27.500274464007695
2	13.616343200174978	20.787001349787264	39.964893508304726	25.63176194173303
3	16.530001012680703	25.28104201607619	30.33801802201725	27.850938949225856
4	11.055686718398155	17.52304751547367	38.220035455582035	33.201230310546144
5	12.384973406101372	38.40007181787136	36.357519729091145	12.857435046936125
6	31.170746461976396	38.1761399009641	18.129952838722907	12.523160798336598
7	27.16650640825518	31.916306721186906	22.993451374175407	17.923735496382506
8	24.84762442078782	36.97741419335745	20.7613692067221	17.41359217913264
9	26.19238194324027	14.756418704121149	19.954777180509797	39.09642217212878
10	14.064867609244395	29.308704918176815	35.27966370903118	21.346763763547607
11	33.98841375534797	22.54957491363952	24.793722029077045	18.668289301935463
12	22.40203039019539	25.047613278914632	32.24400377854543	20.306352552344553
13	28.498148411018075	21.80556396824949	26.128604633850532	23.5676829868819
14	20.722186182065013	21.7201928505473	27.932164769125418	29.625456198262274
15	22.90509664374355	30.497466867215017	23.563244166997652	23.034192322043783
16	22.223876504632788	28.2240050454586	27.30410586215522	22.248012587753387
17	21.637721688870432	28.563566648985052	27.748424031399594	22.05028763074493
18	21.622954148222185	27.82746925585502	29.09354202983277	21.456034566090025
19	23.12833229375555	27.302117640748737	27.67123325674828	21.898316808747435
20	23.263808775639387	27.360284676315246	27.89955505454431	21.47635149350106
21	22.96244132974958	26.98010794724983	27.277233376627517	22.780217346373067
22	22.891837006458484	27.76950122437505	27.20230300878147	22.136358760384994
23	22.191241397041008	27.566368325944175	27.54010398459652	22.702286292418297
24	22.628502679706315	27.203723799981226	28.008398617122655	22.159374903189804
25	22.63144704804197	27.19793557598854	27.635992220957363	22.53462515501213
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	140.0
2	281.0
3	422.0
4	422.0
5	422.0
6	967.5
7	1513.0
8	1513.0
9	1513.0
10	2399.5
11	3286.0
12	3286.0
13	3286.0
14	6219.0
15	9152.0
16	9152.0
17	9152.0
18	17352.5
19	25553.0
20	25553.0
21	25553.0
22	41039.0
23	56525.0
24	56525.0
25	56525.0
26	79725.5
27	102926.0
28	102926.0
29	102926.0
30	131450.0
31	159974.0
32	159974.0
33	159974.0
34	184531.0
35	209088.0
36	209088.0
37	209088.0
38	228367.5
39	247647.0
40	247647.0
41	247647.0
42	260026.0
43	272405.0
44	272405.0
45	272405.0
46	280788.0
47	289171.0
48	289171.0
49	289171.0
50	276963.0
51	264755.0
52	264755.0
53	264755.0
54	234385.5
55	204016.0
56	204016.0
57	204016.0
58	173512.0
59	143008.0
60	143008.0
61	143008.0
62	116159.0
63	89310.0
64	89310.0
65	89310.0
66	68667.0
67	48024.0
68	48024.0
69	48024.0
70	35087.5
71	22151.0
72	22151.0
73	22151.0
74	15535.5
75	8920.0
76	8920.0
77	8920.0
78	6094.0
79	3268.0
80	3268.0
81	3268.0
82	2171.0
83	1074.0
84	1074.0
85	1074.0
86	693.5
87	313.0
88	313.0
89	313.0
90	196.5
91	80.0
92	80.0
93	80.0
94	47.5
95	15.0
96	15.0
97	15.0
98	8.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18421102519631374
2	0.00804536104020045
3	0.007398033140414206
4	0.0014333689209552526
5	0.07929766772381477
6	0.3308307944978978
7	0.027927575105063628
8	0.014657353159445646
9	0.005178623198289945
10	0.003976442812972636
11	0.0
12	0.0
13	0.0
14	0.0012021803853173086
15	0.0
16	0.0
17	0.019234886165076938
18	0.028574903004849876
19	0.0
20	0.0
21	6.473278997862431E-4
22	4.623770712758879E-5
23	0.005039910076907178
24	0.0
25	1.3871312138276636E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2162737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.50583163758057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66865717578126	58.397852500787
2	12.347634019677164	17.658556786669948
3	3.237812726773387	6.945674751440204
4	1.173495239448012	3.3564701207788734
5	0.5441243546135207	1.9454032245450799
6	0.29632744612391787	1.2713484283278655
7	0.17382242453002217	0.8700521916295874
8	0.11311451174020974	0.6470677785810054
9	0.07625086226370437	0.4907143187323911
>10	0.33925139666407306	4.048679351839029
>50	0.015322164686855919	0.7507017263648154
>100	0.011857614622195138	1.6995306553000886
>500	0.0014239274351433141	0.7040791832777921
>1k	9.061356405451945E-4	1.213868981726259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3168	0.1464810561802013	No Hit
CCCATGTACTCTGCGTTGATACCAC	2531	0.11702763673992723	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2470	0.11420713660514432	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2365	0.10935217735674749	No Hit
GTCCTACAGTGGACATTTCTAAATT	2266	0.1047746443511162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.623770712758879E-5	0.0	0.0	0.0	0.0
11	4.623770712758879E-5	0.0	0.0	0.0	0.0
12	4.623770712758879E-5	0.0	0.0	0.0	0.0
13	4.623770712758879E-5	0.0	0.0	0.0	4.623770712758879E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGTG	1235	0.0	10.715257	6
AGGACGT	1290	0.0	10.702207	5
TAGGACC	590	0.0	10.650916	4
GTCCTAG	290	0.0	10.510865	1
GGCGAGG	680	0.0	10.4657135	19
TAGGACG	1355	0.0	10.39962	4
GTAAGAC	215	1.8189894E-12	10.185785	3
GTAGGAC	1350	0.0	10.0857315	3
TGTAGGA	1360	0.0	9.942252	2
TTAGGAC	980	0.0	9.910128	3
TGGCGAG	1350	0.0	9.8419485	18
GACGTGA	705	0.0	9.828354	7
GTCCTAA	675	0.0	9.737149	1
ACGTGGA	665	0.0	9.561143	8
TCTACAC	300	0.0	9.521495	3
ACCGTGC	100	2.785873E-4	9.489792	8
GACGTGG	655	0.0	9.41931	7
CTGTAGG	1470	0.0	9.395874	1
GTCTTAG	315	0.0	9.374274	1
TAAAGGT	315	0.0	9.370142	4
>>END_MODULE