FastQCFastQC Report
Thu 26 May 2016
SRR1513119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC11850.011.3648831
GGACCGA1355.811853E-810.540766
TAGACTG4400.010.3757135
TAGACGT750.002583648310.163784
ATAAACG750.002583648310.163784
GTCCTAA8750.010.0425511
CTACACT4700.09.9340134
GTAGGAC11600.09.8574533
TTAGGAC8550.09.8074943
TAGGACC5450.09.7907984
ACTAGAC2053.8198777E-119.7612833
AGGACGT10550.09.7364525
TAGGACG10800.09.7049994
GTCTAGG3650.09.6821791
GGCGAGG6300.09.64089219
TCCTACA13750.09.63927652
GGACGTG10600.09.576186
GACGTGA5850.09.5685847
GTACTAA2600.09.5513391
GTGTACG900.00106053029.5513391
Produced by FastQC (version 0.11.5)