##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513119_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2925801
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.293982741820102	31.0	30.0	34.0	23.0	34.0
2	29.16148500872069	31.0	30.0	34.0	20.0	34.0
3	29.34859069362544	31.0	30.0	34.0	22.0	34.0
4	32.41075760108087	35.0	33.0	37.0	20.0	37.0
5	32.56084197113884	37.0	33.0	37.0	23.0	37.0
6	32.91080801462574	37.0	35.0	37.0	26.0	37.0
7	32.866524756810186	37.0	35.0	37.0	26.0	37.0
8	32.74504554479269	37.0	35.0	37.0	25.0	37.0
9	33.97984927888124	39.0	35.0	39.0	23.0	39.0
10	33.57019838328034	39.0	34.0	39.0	17.0	39.0
11	34.00466573085456	39.0	34.0	39.0	25.0	39.0
12	33.986827538851756	39.0	35.0	39.0	25.0	39.0
13	34.02284194994807	39.0	35.0	39.0	25.0	39.0
14	34.261634335349534	39.0	33.0	41.0	16.0	41.0
15	34.67700844999369	40.0	34.0	41.0	18.0	41.0
16	34.52169371737859	39.0	34.0	41.0	17.0	41.0
17	34.1337103924703	39.0	33.0	41.0	16.0	41.0
18	33.86542283634464	39.0	33.0	41.0	11.0	41.0
19	34.49384937663225	39.0	34.0	41.0	18.0	41.0
20	34.69594787888855	40.0	34.0	41.0	18.0	41.0
21	34.738740262922875	40.0	35.0	41.0	18.0	41.0
22	34.428193168298186	40.0	34.0	41.0	15.0	41.0
23	33.97130016703118	39.0	34.0	41.0	10.0	41.0
24	34.210583699985065	39.0	34.0	41.0	2.0	41.0
25	33.858000253605766	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9516227747958013
1101	2	1.199355983518597
1101	3	1.1121730660972773
1101	4	1.415111663051988
1101	5	1.2835359861309854
1101	6	1.0347128809068664
1101	7	1.0094368225810442
1101	8	1.0704214240910446
1101	9	1.4211579359423183
1101	10	1.7824878166890343
1101	11	1.3466456398023325
1101	12	1.32675454939735
1101	13	1.2232067719711708
1101	14	2.1881613959528394
1101	15	1.774424764463845
1101	16	1.842270995664407
1101	17	2.2174588645904194
1101	18	2.2486799203419494
1101	19	1.8110796199399672
1101	20	1.4980436418905825
1101	21	1.3784277374775087
1101	22	1.7074688990317526
1101	23	1.8820078715432231
1101	24	1.580310691373306
1101	25	1.7610775653458148
1102	1	1.029471163356856
1102	2	1.1562329694468758
1102	3	1.1087686176179332
1102	4	1.4221475232426428
1102	5	1.3436812425994376
1102	6	1.0549126085509855
1102	7	1.0180614253953877
1102	8	1.0910750781990828
1102	9	1.3809854438860327
1102	10	1.5614256287634802
1102	11	1.2694781409371458
1102	12	1.1889878675998062
1102	13	1.1149453103279612
1102	14	2.131647551195684
1102	15	1.7029313463975697
1102	16	1.814127554901809
1102	17	2.124857865952201
1102	18	2.410277741494923
1102	19	1.8029089435895358
1102	20	1.606305103533792
1102	21	1.5354862939913545
1102	22	1.741286420593262
1102	23	2.009788171134687
1102	24	1.8099974821132037
1102	25	2.1053807882237052
1103	1	0.8577351358194747
1103	2	1.1264121446530098
1103	3	0.9187023715650504
1103	4	1.4152717329426494
1103	5	1.2074041487347884
1103	6	0.9470483840994035
1103	7	0.9176545201060549
1103	8	0.8900543002408625
1103	9	1.3337028802281026
1103	10	1.6187161165509565
1103	11	1.174470115797618
1103	12	1.1384417540720122
1103	13	1.0793672261120264
1103	14	1.9506415907825243
1103	15	1.6751855551122148
1103	16	1.7118830926191393
1103	17	2.0125936263285027
1103	18	2.1951589599473422
1103	19	1.6248080507025762
1103	20	1.3457820285262585
1103	21	1.3378404860908901
1103	22	1.6269260178231946
1103	23	1.978908699948711
1103	24	1.648570141062713
1103	25	1.7861229889136254
1104	1	0.8182336381222939
1104	2	1.0532634251734798
1104	3	0.9218835750255607
1104	4	1.250012513322666
1104	5	1.1775676061370248
1104	6	0.9189622390572723
1104	7	0.8199230292709174
1104	8	0.8088570889851638
1104	9	1.1648592893224006
1104	10	1.2340908260062022
1104	11	0.9493160535101666
1104	12	0.9363083015781655
1104	13	0.9284946362456452
1104	14	1.8550689741596358
1104	15	1.4639377491763952
1104	16	1.4642268658452764
1104	17	1.8647816536092208
1104	18	2.0308037584756704
1104	19	1.4643750138750562
1104	20	1.1830372231419517
1104	21	1.198337744610349
1104	22	1.4154873973184863
1104	23	1.8351523710632733
1104	24	1.5452135641486677
1104	25	1.7216093112403854
1105	1	0.5435430863838953
1105	2	0.7565105941145376
1105	3	0.6817406403466784
1105	4	1.0046020495379508
1105	5	0.876065146853378
1105	6	0.6040777080905784
1105	7	0.5555847296558127
1105	8	0.5560156422027163
1105	9	0.7728602615048672
1105	10	1.1247358064432618
1105	11	0.6351852124629929
1105	12	0.6270209272262832
1105	13	0.48578853952840007
1105	14	1.2712813095446904
1105	15	0.9270655424613778
1105	16	1.0358977870527752
1105	17	1.3005795887652454
1105	18	1.0049597092661458
1105	19	0.9221465092467511
1105	20	0.7787859129819452
1105	21	0.7879392193853008
1105	22	0.8887861774183676
1105	23	1.1639730083696307
1105	24	1.0712167610185972
1105	25	1.3063673690226167
1106	1	0.5963621284436798
1106	2	0.7927810045468888
1106	3	0.7527211452070617
1106	4	1.17015388568268
1106	5	1.092953340172734
1106	6	0.7737360517482514
1106	7	0.6991235769826005
1106	8	0.854571971422196
1106	9	0.9960787663017143
1106	10	0.8574046094547967
1106	11	0.7318748706478146
1106	12	0.8621102427223306
1106	13	0.7292899114747797
1106	14	1.733901578215871
1106	15	1.2873781218793994
1106	16	1.3897223605779772
1106	17	1.6721633727958718
1106	18	1.779842269862094
1106	19	1.307626114698003
1106	20	1.0770933659664763
1106	21	0.9173324756037289
1106	22	1.1733002582341499
1106	23	1.6252407384436438
1106	24	1.2457620928270998
1106	25	1.5742525563697356
1107	1	0.5313506569237774
1107	2	0.7674789900265573
1107	3	0.6259776848923337
1107	4	0.942108992439529
1107	5	0.8801854117944998
1107	6	0.5944853952712066
1107	7	0.6439491209022066
1107	8	0.5974151228736773
1107	9	0.8245623138265685
1107	10	1.1098377282384178
1107	11	0.681659607041972
1107	12	0.6711566054089104
1107	13	0.5924124147783445
1107	14	1.5328110390492427
1107	15	0.9590171226657773
1107	16	1.215820304271169
1107	17	1.5467766144262143
1107	18	1.6957027385929564
1107	19	1.1213772357519716
1107	20	0.885092497629806
1107	21	0.821428652289093
1107	22	1.0638930981026604
1107	23	1.4198473973942924
1107	24	1.130700764573362
1107	25	1.4474245646458144
1108	1	0.7389679850667719
1108	2	1.0060863286636774
1108	3	0.7873463267701837
1108	4	1.2638788985754417
1108	5	1.1191043439151827
1108	6	0.7685217303803427
1108	7	0.6643771562008993
1108	8	0.7738458726991695
1108	9	1.1166498385058787
1108	10	1.2829931519346545
1108	11	0.8975838953403965
1108	12	0.6730458132445207
1108	13	0.7530995197715598
1108	14	1.560717416434258
1108	15	1.1391460262506357
1108	16	1.1849352743881312
1108	17	1.5824865669820696
1108	18	1.7767017305427402
1108	19	1.1592665700370262
1108	20	1.0263432204794114
1108	21	1.0560765674096118
1108	22	1.2100564408428909
1108	23	1.580851807761725
1108	24	1.1548730426801939
1108	25	1.5598580605119778
1109	1	0.4168796960772134
1109	2	0.5878943951629729
1109	3	0.5449917998249703
1109	4	0.7962128022767558
1109	5	0.9079337805131686
1109	6	0.5861075602423824
1109	7	0.5372705640093329
1109	8	0.5077800209106869
1109	9	0.7071444749430427
1109	10	1.06872515729237
1109	11	0.6890809418160373
1109	12	0.5720616070611726
1109	13	0.4922748003972828
1109	14	1.446829511274295
1109	15	0.9493505288850841
1109	16	0.9996108250036144
1109	17	1.3952048379255544
1109	18	1.6028235029378664
1109	19	0.9763508442437683
1109	20	0.7839391661916011
1109	21	0.6286152776299545
1109	22	1.0089491321849806
1109	23	1.3454300860919517
1109	24	1.0030618029246128
1109	25	1.2277325222856348
1110	1	0.37106469880761495
1110	2	0.6056979278651262
1110	3	0.5461429737060897
1110	4	0.7345983333745991
1110	5	0.6716250650977855
1110	6	0.31640363517553993
1110	7	0.4279770587123011
1110	8	0.33389933606908784
1110	9	0.48043267779339516
1110	10	0.8180672490969982
1110	11	0.49872671614003394
1110	12	0.49843018438478737
1110	13	0.3561342894110453
1110	14	1.1111535419809329
1110	15	0.8218362649617674
1110	16	0.9436186053910589
1110	17	1.2421045359566136
1110	18	0.902929999518058
1110	19	0.5533642373018282
1110	20	0.3911502241226117
1110	21	0.374665915129313
1110	22	0.717986419946925
1110	23	-0.6420281064793798
1110	24	0.10378013138316788
1110	25	0.5036068953095807
1111	1	0.2770067616972618
1111	2	0.5793638879467657
1111	3	0.6013308649897695
1111	4	0.9965589482347283
1111	5	0.7772462434755667
1111	6	0.4858255541917842
1111	7	0.5057498683762276
1111	8	0.562363957573929
1111	9	0.7252550645436528
1111	10	0.9893086424359225
1111	11	0.6675479305744005
1111	12	0.5715429191550001
1111	13	0.44362592211370355
1111	14	1.3921631930786376
1111	15	0.9092225418548665
1111	16	0.9652512675043923
1111	17	1.316670408257643
1111	18	1.6722679772826865
1111	19	1.0813363478209794
1111	20	0.7518806942484701
1111	21	0.5348124212297165
1111	22	0.7873653429186689
1111	23	1.1424603610180668
1111	24	0.8402787653108419
1111	25	1.01678097396492
1112	1	0.0974368701507018
1112	2	0.23879558594768113
1112	3	0.19334955007151677
1112	4	0.6283809476407285
1112	5	0.39220077161779443
1112	6	0.055224208907262096
1112	7	-0.03325062968580994
1112	8	0.11635672619567572
1112	9	0.29842192911385723
1112	10	0.5478330862522824
1112	11	0.1932884603584526
1112	12	0.21514463152330876
1112	13	0.1562156896100717
1112	14	0.7459104636469647
1112	15	0.4269122176090079
1112	16	0.3825548733701467
1112	17	0.7865182666770423
1112	18	0.9483606547366819
1112	19	0.453590472056149
1112	20	0.3060406324818885
1112	21	0.23902230928352708
1112	22	0.5413453871180849
1112	23	0.8687408026905246
1112	24	0.614251375919963
1112	25	0.9410900493216516
1113	1	-0.05270978497904366
1113	2	0.11046623839368763
1113	3	0.00830245668883478
1113	4	0.31630216986173565
1113	5	0.32848905573546006
1113	6	0.03474386429272158
1113	7	-0.025289942188422287
1113	8	0.06228034084633549
1113	9	0.1417979255827433
1113	10	0.49046677340891875
1113	11	0.16769861215671256
1113	12	-0.05703719736352042
1113	13	-0.21275424377940766
1113	14	0.3748975177048308
1113	15	0.2302510461804772
1113	16	0.26260282971302473
1113	17	0.5931287437641544
1113	18	0.5389381877172497
1113	19	0.2078307140159481
1113	20	0.06372117058422333
1113	21	-0.036346699997771736
1113	22	0.09756844606855708
1113	23	0.2441872667375975
1113	24	0.19326062450579684
1113	25	0.4963097366664613
1114	1	-0.9542847818379308
1114	2	-0.6063590895193656
1114	3	-0.5758271991006048
1114	4	-0.30638420946370815
1114	5	-3.357010286303513
1114	6	-1.2509863037269895
1114	7	-0.7965320072802342
1114	8	-0.5914174415973719
1114	9	-0.35847504270514463
1114	10	-0.2540121931874353
1114	11	-0.6536131041493078
1114	12	-0.7523305492001313
1114	13	-0.7502132031010049
1114	14	-6.217398235140482
1114	15	-2.493382559495906
1114	16	-1.3632562875456173
1114	17	-0.4010831220428983
1114	18	-6.4072131149475595
1114	19	-2.465930520358711
1114	20	-1.3737579220337466
1114	21	-1.0758897759676884
1114	22	-0.7460252493065624
1114	23	-0.3719014992317753
1114	24	-0.47387387109809964
1114	25	-0.3062221667378324
1115	1	-0.8077961993303902
1115	2	-0.43271150503526457
1115	3	-0.5185428212148118
1115	4	-3.0404636611553784
1115	5	-1.0951136416150682
1115	6	-0.6969251658167366
1115	7	-0.5927646446706163
1115	8	-0.4051366524326596
1115	9	-0.25741960767248173
1115	10	-0.5670817902517413
1115	11	-0.5630852042535679
1115	12	-0.6304315859457859
1115	13	-0.693831603268869
1115	14	-6.2077288763611875
1115	15	-2.510978741157352
1115	16	-1.382627688938129
1115	17	-0.4679034147672638
1115	18	-0.23659781378498934
1115	19	-0.5233255899030524
1115	20	-0.5911209469749465
1115	21	-0.8123592104434678
1115	22	-1.995363175952484
1115	23	-1.0407663192558019
1115	24	-0.7448097627510109
1115	25	-0.3619124402816567
1116	1	-0.5574851250873358
1116	2	-0.2923713902428702
1116	3	-0.39065827904065387
1116	4	-0.3073332025803168
1116	5	-0.8014496476894912
1116	6	-0.6145023120551798
1116	7	-0.5211467318006058
1116	8	-0.5116903148690426
1116	9	-0.5332946571714388
1116	10	-0.32864490399105506
1116	11	-0.5973009373266649
1116	12	-0.8305356771761581
1116	13	-0.8623123337014391
1116	14	-0.08086760243610769
1116	15	-0.527671581286917
1116	16	-0.595619588665663
1116	17	-0.18421804632985328
1116	18	-0.2038475802402857
1116	19	-0.5129108172923225
1116	20	-0.8440322159805476
1116	21	-1.0000000804013922
1116	22	-0.9709100808028737
1116	23	-1.6082989816167412
1116	24	-1.1193658365994636
1116	25	-0.586788349558887
1201	1	0.9536006118081666
1201	2	1.183243174076683
1201	3	1.0768162414663074
1201	4	1.4746072226154894
1201	5	1.316702026886098
1201	6	0.99311158573326
1201	7	0.9171209933303857
1201	8	1.0522123775496723
1201	9	1.227372518490526
1201	10	1.7419282689885804
1201	11	1.1767901898458177
1201	12	1.1305372665320519
1201	13	1.109245388442453
1201	14	1.964126973382875
1201	15	1.665433687131106
1201	16	1.7479223852369827
1201	17	2.1489259583876503
1201	18	1.491459681195181
1201	19	1.4857910025257652
1201	20	1.4373347745038743
1201	21	1.365186550638704
1201	22	1.6870792623370434
1201	23	1.953834879179034
1201	24	1.6499393717785935
1201	25	1.9799073280390118
1202	1	0.8616345492128623
1202	2	0.9830517832033507
1202	3	0.9540059401801919
1202	4	1.332247804164318
1202	5	1.1826774323435458
1202	6	0.885695496398192
1202	7	0.854317881029317
1202	8	0.9721315935698129
1202	9	1.1211260736006565
1202	10	1.500779140630371
1202	11	1.0650024789034092
1202	12	1.0273882152777674
1202	13	1.017949505943207
1202	14	1.9317835867106012
1202	15	1.573704321516253
1202	16	1.5525787276943817
1202	17	1.9275310879328202
1202	18	2.0863707917403715
1202	19	1.5335179314149059
1202	20	1.3426613171888988
1202	21	1.2078529151975559
1202	22	1.3179359020773944
1202	23	1.612838227361756
1202	24	1.2494704268955275
1202	25	1.737007356696303
1203	1	0.6358974943633022
1203	2	0.7268693457232906
1203	3	0.623381164799703
1203	4	0.9281783043642093
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2211	15	-0.5898458001599423
2211	16	-6.918240695012511
2211	17	-2.249868585182874
2211	18	-1.0593294957729569
2211	19	-1.0599761790987827
2211	20	-1.1163233475989145
2211	21	-0.9901910167447738
2211	22	-0.8093916493161473
2211	23	-0.5099064597946992
2211	24	-0.9819931806470379
2211	25	-7.873306555825341
2212	1	-1.469888823687164
2212	2	-3.7128305600143534
2212	3	-1.7289939375753995
2212	4	-3.707945426570845
2212	5	-1.370041429353435
2212	6	-1.125320723666345
2212	7	-0.973840503130269
2212	8	-0.8407173123427398
2212	9	-0.9213342527073394
2212	10	-0.43956981717839483
2212	11	-0.9694628517369637
2212	12	-1.0279812934304147
2212	13	-1.1936998671121302
2212	14	-6.366555234807183
2212	15	-4.944618404487901
2212	16	-2.7035150406855273
2212	17	-7.341670702217353
2212	18	-2.9843574005108735
2212	19	-1.9561033069747609
2212	20	-1.9015578293606126
2212	21	-1.514096510215289
2212	22	-1.455331379552959
2212	23	-0.8958461229707311
2212	24	-1.09234915419826
2212	25	-0.8996655426411877
2213	1	-0.2574990855782673
2213	2	-0.2632004779203889
2213	3	-0.2838635162140797
2213	4	0.07388564158257793
2213	5	-0.12657742227104762
2213	6	-0.36291234769404923
2213	7	-0.36025401033933235
2213	8	-0.3496693111079807
2213	9	-0.2065445256646754
2213	10	-3.9394271210367258
2213	11	-1.5018403150655075
2213	12	-1.014412419244728
2213	13	-0.7874443157953905
2213	14	0.06734114158447113
2213	15	-0.41327257602031153
2213	16	-0.25430503855964304
2213	17	0.10278482463718319
2213	18	0.20455117785697752
2213	19	-0.3599690619392746
2213	20	-0.6864423037291871
2213	21	-0.6523071879637214
2213	22	-0.9636346933068296
2213	23	-1.1334719649507718
2213	24	-0.6701214635751498
2213	25	-0.21937806815904537
2214	1	-0.9080073925722054
2214	2	-0.7396624712045146
2214	3	-0.5978813331787407
2214	4	-0.1266969774646114
2214	5	-3.3811274444122716
2214	6	-1.2203565957259848
2214	7	-0.9735783228407904
2214	8	-0.7432204322429072
2214	9	-0.6509614404863839
2214	10	-4.270792365226672
2214	11	-1.7554798576833335
2214	12	-1.255777430369129
2214	13	-1.0942779769145048
2214	14	-0.20220333180392913
2214	15	-0.5128742580364332
2214	16	-0.5000606908473628
2214	17	-0.12599186809099194
2214	18	0.21799099226508645
2214	19	-0.6031910228093622
2214	20	-0.9765827795056055
2214	21	-0.9277477714881357
2214	22	-0.6470327620898502
2214	23	-0.5843237747047425
2214	24	-1.0578317380008642
2214	25	-0.7985544234800201
2215	1	-2.3683447225371417
2215	2	-4.808479610828201
2215	3	-3.091095970563508
2215	4	-2.0386280589611445
2215	5	-2.065086337474529
2215	6	-2.2211031237358227
2215	7	-2.3481694144068754
2215	8	-2.542606456953404
2215	9	-8.406184908778439
2215	10	-3.9977137113385304
2215	11	-3.28909522756112
2215	12	-3.708681114666419
2215	13	-3.029876959266055
2215	14	-8.230991332106907
2215	15	-4.92615269894911
2215	16	-9.382807004990624
2215	17	-4.720130364519864
2215	18	-3.1968994679490486
2215	19	-3.804367957299359
2215	20	-3.498255037892072
2215	21	-3.742959477118621
2215	22	-9.728667861159085
2215	23	-10.905641593800354
2215	24	-6.946617777860247
2215	25	-5.319746818741095
2216	1	-1.9068019988332914
2216	2	-1.8485676695853073
2216	3	-1.7543385184295097
2216	4	-6.984793884892159
2216	5	-3.5300404203735134
2216	6	-2.542191081115245
2216	7	-2.157681263361109
2216	8	-7.829523141972928
2216	9	-3.779321394608285
2216	10	-2.1104554883845275
2216	11	-2.0835381571665508
2216	12	-2.2719332759338506
2216	13	-2.1245490374037814
2216	14	-1.509880477353164
2216	15	-2.0212587784641727
2216	16	-1.6746766892166107
2216	17	-1.458226002133081
2216	18	-7.3542547687785955
2216	19	-4.612338341031386
2216	20	-3.9851397365032
2216	21	-3.2320818260046913
2216	22	-2.838722255532275
2216	23	-2.1168557139643767
2216	24	-2.4846611535275045
2216	25	-1.909657641333638
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	146266.0
3	2.0
4	1187.0
5	7052.0
6	8586.0
7	7686.0
8	6917.0
9	6594.0
10	6183.0
11	5879.0
12	5797.0
13	5580.0
14	5733.0
15	5743.0
16	5919.0
17	6172.0
18	7127.0
19	8718.0
20	11111.0
21	14121.0
22	17617.0
23	20618.0
24	24148.0
25	27532.0
26	30568.0
27	34934.0
28	38970.0
29	46315.0
30	55403.0
31	65970.0
32	84253.0
33	108903.0
34	143389.0
35	192091.0
36	273365.0
37	395831.0
38	1093521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.784884975022855	18.402900704290452	12.330424183492143	25.481790137194547
2	14.651939843121156	19.814152265888772	41.13836020454353	24.39554768644654
3	18.322648693993028	24.68422401693094	29.512118395947102	27.481008893128934
4	11.386724329253555	16.382611131864028	38.87819579643587	33.35246874244655
5	12.763823469143695	37.94980286697822	36.142675924003214	13.143697739874874
6	32.24968592696413	37.04646571689841	17.924195161138694	12.77965319499876
7	28.29916940687987	31.362385347758952	22.37429232761954	17.964152917741643
8	25.476809942956802	36.311872142840784	20.56905091294067	17.64226700126174
9	26.48851726000424	14.80196632094241	19.931424898983337	38.778091520070014
10	13.86133435251977	28.80789030800876	35.53233290339254	21.798442436078926
11	34.906195089600104	22.30621970313344	23.995870396072775	18.79171481119369
12	22.770618596352197	24.62296490965961	31.74537511713703	20.86104137685116
13	28.84987047867676	21.52484734193253	25.73229889494055	23.89298328445016
14	20.622710960824676	20.914224255670185	28.65353130391945	29.809533479585692
15	23.45331214276824	29.69678982086952	23.25608670260127	23.593811333760975
16	22.696664244051888	27.722894803593856	26.95994207603273	22.620498876321527
17	21.916103250698992	27.83806582230351	27.839433544270253	22.406397382727246
18	21.933071648189042	27.3434991892	28.8688904091745	21.854538753436454
19	23.528335855251687	26.998292662414165	27.23155586522084	22.241815617113307
20	23.69114368940005	27.012191786442024	27.390732054210034	21.905932469947896
21	23.627309357781854	26.536275315963398	26.765317792589883	23.071097533664865
22	23.372165268185864	27.063484383393575	27.054901669799413	22.509448678621144
23	22.765660224864767	26.85033426120991	27.42135100743327	22.962654506492058
24	23.164142418415736	26.79494277226985	27.377837748929768	22.66307706038465
25	23.057770507878946	26.694767621764058	27.286282697021534	22.96117917333546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	188.0
1	188.0
2	379.5
3	571.0
4	571.0
5	571.0
6	1236.0
7	1901.0
8	1901.0
9	1901.0
10	3247.0
11	4593.0
12	4593.0
13	4593.0
14	8656.5
15	12720.0
16	12720.0
17	12720.0
18	23013.5
19	33307.0
20	33307.0
21	33307.0
22	51743.5
23	70180.0
24	70180.0
25	70180.0
26	98649.0
27	127118.0
28	127118.0
29	127118.0
30	161455.0
31	195792.0
32	195792.0
33	195792.0
34	229226.0
35	262660.0
36	262660.0
37	262660.0
38	291121.5
39	319583.0
40	319583.0
41	319583.0
42	340147.5
43	360712.0
44	360712.0
45	360712.0
46	373869.5
47	387027.0
48	387027.0
49	387027.0
50	377107.0
51	367187.0
52	367187.0
53	367187.0
54	331955.5
55	296724.0
56	296724.0
57	296724.0
58	255744.5
59	214765.0
60	214765.0
61	214765.0
62	176105.5
63	137446.0
64	137446.0
65	137446.0
66	106677.5
67	75909.0
68	75909.0
69	75909.0
70	55723.5
71	35538.0
72	35538.0
73	35538.0
74	24845.0
75	14152.0
76	14152.0
77	14152.0
78	9756.0
79	5360.0
80	5360.0
81	5360.0
82	3532.5
83	1705.0
84	1705.0
85	1705.0
86	1093.5
87	482.0
88	482.0
89	482.0
90	308.0
91	134.0
92	134.0
93	134.0
94	84.5
95	35.0
96	35.0
97	35.0
98	23.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17537077880553054
2	0.25627170132213367
3	0.2595186753986344
4	0.45201297012339525
5	0.25521216241295974
6	0.017977982781467367
7	0.01852484157329907
8	0.016200691708014317
9	0.01849066289880959
10	0.020233775297773156
11	0.01985780987838886
12	0.0276163689875012
13	0.02734293959158535
14	0.02460864563242681
15	0.04200559094757299
16	0.02006288192532575
17	0.04203976962206247
18	0.031170951134407297
19	0.026625187427306234
20	0.030111412225233364
21	0.03472553328131339
22	0.04504749297713686
23	0.028812622594633058
24	0.048943861868937774
25	0.037869971334345706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2925801.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.96046291425718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10909520316994	60.636780584083496
2	11.245220613529986	16.409130030721897
3	2.9082652473632975	6.365651361752185
4	1.098181657454967	3.204953683674424
5	0.5186963657259314	1.8922163477653398
6	0.30056226771069977	1.3157497312038928
7	0.1837994875643392	0.9387066987268209
8	0.11981840601539004	0.6993605094825018
9	0.09204879468704422	0.6044330403959546
>10	0.40074743003092594	4.889602471842097
>50	0.01410324108753571	0.710199036807064
>100	0.007759525849971516	1.0414573465635841
>500	0.0011345065403453403	0.5662439752569135
>1k	5.672532695577166E-4	0.7255151817238427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.835734897896337E-5	0.0	0.0	0.0
6	1.3671469795792674E-4	6.835734897896337E-5	0.0	0.0	0.0
7	1.3671469795792674E-4	6.835734897896337E-5	0.0	0.0	0.0
8	1.3671469795792674E-4	6.835734897896337E-5	0.0	0.0	0.0
9	1.3671469795792674E-4	6.835734897896337E-5	0.0	0.0	0.0
10	1.3671469795792674E-4	6.835734897896337E-5	0.0	0.0	0.0
11	1.3671469795792674E-4	1.0253602346844505E-4	0.0	3.4178674489481686E-5	0.0
12	1.3671469795792674E-4	1.0253602346844505E-4	0.0	3.4178674489481686E-5	0.0
13	1.3671469795792674E-4	1.0253602346844505E-4	0.0	3.4178674489481686E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	1185	0.0	11.364883	1
GGACCGA	135	5.811853E-8	10.54076	6
TAGACTG	440	0.0	10.375713	5
TAGACGT	75	0.0025836483	10.16378	4
ATAAACG	75	0.0025836483	10.16378	4
GTCCTAA	875	0.0	10.042551	1
CTACACT	470	0.0	9.934013	4
GTAGGAC	1160	0.0	9.857453	3
TTAGGAC	855	0.0	9.807494	3
TAGGACC	545	0.0	9.790798	4
ACTAGAC	205	3.8198777E-11	9.761283	3
AGGACGT	1055	0.0	9.736452	5
TAGGACG	1080	0.0	9.704999	4
GTCTAGG	365	0.0	9.682179	1
GGCGAGG	630	0.0	9.640892	19
TCCTACA	1375	0.0	9.6392765	2
GGACGTG	1060	0.0	9.57618	6
GACGTGA	585	0.0	9.568584	7
GTACTAA	260	0.0	9.551339	1
GTGTACG	90	0.0010605302	9.551339	1
>>END_MODULE