FastQCFastQC Report
Thu 26 May 2016
SRR1513119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39510.13503994290794213No Hit
TATCAACGCAGAGTACTTTTTTTTT30430.10400570647149276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC400.005206653414.2803124
TAGGACC6050.011.959274
GGCGAGG5950.011.80100219
TAGACGG1052.648776E-711.7869245
GTCCTAC11250.011.4287571
TGTGCGA951.3796458E-510.98666310
GGACCGT803.7023862E-410.7093156
TCCTACA13300.010.5232372
AGGACGT12400.010.4415195
GGACGTG12300.010.2159336
TGGCGAG11900.010.20906418
TTAGCGC750.0026024810.1548884
AATATCG856.469021E-410.080225
CCGATCC951.6652071E-49.9878769
TAGGACG13750.09.9702544
GTAGGAC13100.09.8109023
GTCCTAT3050.09.6800951
GACGTGA6750.09.4209347
GGTATCA16550.09.2650041
ATTATAC3600.09.2557573
Produced by FastQC (version 0.11.5)