##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513119_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2925801 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.666325563495263 31.0 31.0 34.0 27.0 34.0 2 30.780457044071007 33.0 31.0 34.0 27.0 34.0 3 30.875797772985926 33.0 31.0 34.0 27.0 34.0 4 34.250637005045796 37.0 35.0 37.0 30.0 37.0 5 33.98469684028408 37.0 35.0 37.0 30.0 37.0 6 33.89960048547389 37.0 35.0 37.0 30.0 37.0 7 33.75454311485983 37.0 35.0 37.0 29.0 37.0 8 33.722867686489955 37.0 35.0 37.0 28.0 37.0 9 35.27227244778438 39.0 35.0 39.0 29.0 39.0 10 35.12083494400337 39.0 35.0 39.0 28.0 39.0 11 35.126785109445244 39.0 35.0 39.0 28.0 39.0 12 35.058845765655285 39.0 35.0 39.0 27.0 39.0 13 35.027174438726355 39.0 35.0 39.0 27.0 39.0 14 36.18060934424453 40.0 36.0 41.0 27.0 41.0 15 36.162106035236164 40.0 36.0 41.0 27.0 41.0 16 36.11825650479988 40.0 36.0 41.0 27.0 41.0 17 36.05162039386821 40.0 36.0 41.0 27.0 41.0 18 36.00792774354783 40.0 36.0 41.0 27.0 41.0 19 36.089706374425326 40.0 37.0 41.0 27.0 41.0 20 36.04797113679297 40.0 37.0 41.0 27.0 41.0 21 35.99888338270443 40.0 37.0 41.0 27.0 41.0 22 35.93646389484452 40.0 37.0 41.0 27.0 41.0 23 35.85762087031893 40.0 36.0 41.0 27.0 41.0 24 35.79378877784237 40.0 36.0 41.0 27.0 41.0 25 35.73595162487128 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49650701358665117 1101 2 0.534066687353203 1101 3 0.4887614406462255 1101 4 0.5875051439266059 1101 5 0.5881642509167477 1101 6 0.46040209180423375 1101 7 0.501239069818979 1101 8 0.5049764643029278 1101 9 0.5459766090306744 1101 10 0.6650158139259972 1101 11 0.6723517194546673 1101 12 0.5948810625814289 1101 13 0.6788337161977225 1101 14 0.6958760470470011 1101 15 0.867284399676663 1101 16 0.7580689930527313 1101 17 0.7512544402355559 1101 18 0.7477298250710191 1101 19 0.771222784765456 1101 20 0.740752615210198 1101 21 0.7368602989018811 1101 22 0.758162901065397 1101 23 0.7379228684246613 1101 24 0.7706689599912764 1101 25 0.7274154064168457 1102 1 0.48561277845274375 1102 2 0.5161365920286478 1102 3 0.4678808233062348 1102 4 0.5747952029370467 1102 5 0.5450412368450301 1102 6 0.5377965539013729 1102 7 0.5591146939678637 1102 8 0.5612633458281238 1102 9 0.501718778799173 1102 10 0.5939763223236341 1102 11 0.577254125264929 1102 12 0.5996472904525163 1102 13 0.5328963580497472 1102 14 0.6570653343824517 1102 15 0.5847151758909135 1102 16 0.6917957293214556 1102 17 0.8393161742346464 1102 18 0.7150471196692934 1102 19 0.7492073512656816 1102 20 0.8088415847971291 1102 21 0.7373142253657932 1102 22 0.7429563645243178 1102 23 0.7295252288422702 1102 24 0.8242322827329929 1102 25 0.7764394645194344 1103 1 0.4177879808746745 1103 2 0.4792565098353876 1103 3 0.5152650153686658 1103 4 0.5333912137438759 1103 5 0.517124862385792 1103 6 0.40426126245873206 1103 7 0.4793315520793584 1103 8 0.5341455686083023 1103 9 0.6253749853674577 1103 10 0.5507098328573292 1103 11 0.6913381936004441 1103 12 0.6212960989676972 1103 13 0.6453305624188985 1103 14 0.800502077194146 1103 15 0.6942806969395434 1103 16 0.7132872508738899 1103 17 0.7922716024489134 1103 18 0.7546640568571661 1103 19 0.7586719860239128 1103 20 0.7358959937176692 1103 21 0.6152711893439928 1103 22 0.7214776238259972 1103 23 0.7232154036225182 1103 24 0.8158922600655245 1103 25 0.7539745366261243 1104 1 0.3685491762900277 1104 2 0.3790501934787862 1104 3 0.3665895233006644 1104 4 0.5364038997274037 1104 5 0.46779959968964846 1104 6 0.34792803894236357 1104 7 0.35960693784938513 1104 8 0.5418098526714346 1104 9 0.4426601763974176 1104 10 0.6530252720918384 1104 11 0.5446077056794039 1104 12 0.4892025142809189 1104 13 0.6172183282624246 1104 14 0.6443610030385685 1104 15 0.6528784859174337 1104 16 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15 -0.7650458057022931 2214 16 -0.8246149825761648 2214 17 -0.8254533322605297 2214 18 -0.8091993600039586 2214 19 -0.8378529164598589 2214 20 -0.8586310609806134 2214 21 -0.7806258400437116 2214 22 -0.8461786874663204 2214 23 -0.8316778632261901 2214 24 -1.0378593452237013 2214 25 -0.9241103281459431 2215 1 -0.5215669208832203 2215 2 -0.5986059627154603 2215 3 -0.6117725454218714 2215 4 -0.3257724924380767 2215 5 -0.6120051575040648 2215 6 -0.6577870480530024 2215 7 -0.7261283865265113 2215 8 -0.7726486000990889 2215 9 -0.8337628525014509 2215 10 -0.9049438306706534 2215 11 -0.8715183579809747 2215 12 -0.896767456603186 2215 13 -0.9562933927886803 2215 14 -1.022076936130908 2215 15 -1.0920642828831788 2215 16 -1.0430832523510247 2215 17 -1.125498778907506 2215 18 -1.1385371756242364 2215 19 -1.007741171174949 2215 20 -1.0263592632983958 2215 21 -0.9905871212827151 2215 22 -1.1197173772662197 2215 23 -1.1169569368274779 2215 24 -1.1329787317830835 2215 25 -1.195029820747493 2216 1 -0.5405292678116496 2216 2 -0.4595065112037062 2216 3 -0.5676361959275376 2216 4 -0.36298264838492855 2216 5 -0.5885288816309071 2216 6 -0.6927609520378439 2216 7 -0.8820605543660136 2216 8 -0.7914641241692948 2216 9 -0.8778976288937343 2216 10 -1.0166024395590298 2216 11 -1.006204034711999 2216 12 -0.9181707335495517 2216 13 -0.9960617622509247 2216 14 -1.1407251870162156 2216 15 -1.093640880717885 2216 16 -1.148069111911468 2216 17 -1.2754547320500365 2216 18 -1.203304408957223 2216 19 -1.192788311799788 2216 20 -1.1933844224438843 2216 21 -1.4066876459996394 2216 22 -1.254352889520682 2216 23 -1.377896638077921 2216 24 -1.3131090545486614 2216 25 -1.3289044759759605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77704.0 3 2.0 4 1287.0 5 10350.0 6 11415.0 7 8681.0 8 7205.0 9 6275.0 10 5656.0 11 5074.0 12 4676.0 13 4444.0 14 4303.0 15 4277.0 16 4340.0 17 4526.0 18 5182.0 19 6219.0 20 7726.0 21 10129.0 22 12768.0 23 15543.0 24 17856.0 25 19738.0 26 22480.0 27 24409.0 28 27532.0 29 32940.0 30 42141.0 31 53381.0 32 68147.0 33 88802.0 34 117496.0 35 157952.0 36 227486.0 37 371176.0 38 1436483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.81536746217067 19.161198882164665 13.344099398449535 27.67933425721513 2 13.92438422466656 20.20977037031973 39.435906726187625 26.429938678826083 3 16.70155935271532 24.691990154622736 29.918031526125766 28.688418966536176 4 11.170708915368953 16.97788888421768 37.395440374909036 34.455961825504325 5 12.779203223037964 38.08185046929094 35.94194946865915 13.196996839011948 6 32.33966977178671 37.508590405357396 17.459373041010355 12.692366781845541 7 27.998446481386324 31.42750377524852 22.381163860315976 18.192885883049176 8 25.510102228964715 36.74954553130279 20.035489236663505 17.704863003068986 9 26.181930293311694 14.643230807144647 19.538572335326844 39.63626656421682 10 14.201363028426448 29.135817829018855 34.81905642120101 21.84376272135369 11 34.92038590457793 22.219932251031427 23.85497168125925 19.004710163131396 12 22.506417900602262 24.82294592147586 31.69624318263614 20.974392995285733 13 28.821679943372775 21.388638530098252 25.731927769523626 24.057753757005347 14 21.062851103913772 21.0176664483767 27.562844951691385 30.356637496018145 15 23.39882309152263 29.824106287474784 23.11968585696703 23.65738476403556 16 22.902036057818012 27.89400236037926 26.375614746184034 22.82834683561869 17 22.179140897923126 28.12871660461429 27.145439124687158 22.54670337277542 18 22.20090004434238 27.58680696226658 28.136317435376196 22.075975558014843 19 23.410307126151096 27.041210253192205 27.18568351025924 22.36279911039746 20 23.617156464161436 26.963385411379654 27.34816892878224 22.071289195676673 21 23.450458594872828 26.454814690758205 26.887450718354224 23.207275996014744 22 23.298721717137195 27.164536195228656 26.850434069314378 22.686308018319775 23 22.86488618605357 26.886451293591996 26.99904158115866 23.249620939195776 24 22.978391216627514 26.83815474805019 27.409724721537792 22.7737293137845 25 23.066998108892648 26.714377850289782 27.08672058470182 23.131903456115747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 120.0 2 290.0 3 460.0 4 460.0 5 460.0 6 1218.5 7 1977.0 8 1977.0 9 1977.0 10 3268.0 11 4559.0 12 4559.0 13 4559.0 14 8401.5 15 12244.0 16 12244.0 17 12244.0 18 22329.0 19 32414.0 20 32414.0 21 32414.0 22 50664.0 23 68914.0 24 68914.0 25 68914.0 26 96512.5 27 124111.0 28 124111.0 29 124111.0 30 158394.0 31 192677.0 32 192677.0 33 192677.0 34 225055.0 35 257433.0 36 257433.0 37 257433.0 38 286212.5 39 314992.0 40 314992.0 41 314992.0 42 336161.5 43 357331.0 44 357331.0 45 357331.0 46 372358.5 47 387386.0 48 387386.0 49 387386.0 50 379514.0 51 371642.0 52 371642.0 53 371642.0 54 336544.0 55 301446.0 56 301446.0 57 301446.0 58 260954.5 59 220463.0 60 220463.0 61 220463.0 62 180750.5 63 141038.0 64 141038.0 65 141038.0 66 109308.5 67 77579.0 68 77579.0 69 77579.0 70 57268.5 71 36958.0 72 36958.0 73 36958.0 74 25668.5 75 14379.0 76 14379.0 77 14379.0 78 9819.5 79 5260.0 80 5260.0 81 5260.0 82 3507.5 83 1755.0 84 1755.0 85 1755.0 86 1141.0 87 527.0 88 527.0 89 527.0 90 325.0 91 123.0 92 123.0 93 123.0 94 67.0 95 11.0 96 11.0 97 11.0 98 6.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.188461211135002 2 0.0075534870621754525 3 0.005605302616274996 4 0.002665936610179571 5 0.080251527701303 6 0.3334813269938728 7 0.028299942477290833 8 0.013910720517219045 9 0.004853371777506399 10 0.0037596541938429854 11 0.0 12 0.0 13 0.0 14 9.911815601949688E-4 15 0.0 16 0.0 17 0.019960345901857305 18 0.028505014524227726 19 0.0 20 0.0 21 5.468587918317069E-4 22 3.417867448948168E-5 23 0.005092622498932771 24 0.0 25 6.835734897896336E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2925801.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.20777395502489 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6692494711341 57.33816205945919 2 11.887433382262603 16.691804716146198 3 3.247543532822419 6.840084067844979 4 1.2486405707028534 3.5065709975591677 5 0.6275327340995739 2.202883817252081 6 0.3280889367396722 1.3820636344658017 7 0.22897971435946596 1.1253309218224887 8 0.14342063089443385 0.8055394587458776 9 0.11138469284913079 0.7038064203841485 >10 0.47849043667894153 5.659623340623094 >50 0.016973813640700185 0.8142555991909636 >100 0.010408535077775343 1.3474169860005152 >500 0.0011218847627847846 0.5652589631754855 >1k 7.316639755531535E-4 1.0171990173300922 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3951 0.13503994290794213 No Hit TATCAACGCAGAGTACTTTTTTTTT 3043 0.10400570647149276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.835734897896337E-5 0.0 0.0 0.0 0.0 7 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 8 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 9 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 10 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 11 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 12 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 13 6.835734897896337E-5 3.4178674489481686E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 40 0.0052066534 14.280312 4 TAGGACC 605 0.0 11.95927 4 GGCGAGG 595 0.0 11.801002 19 TAGACGG 105 2.648776E-7 11.786924 5 GTCCTAC 1125 0.0 11.428757 1 TGTGCGA 95 1.3796458E-5 10.986663 10 GGACCGT 80 3.7023862E-4 10.709315 6 TCCTACA 1330 0.0 10.523237 2 AGGACGT 1240 0.0 10.441519 5 GGACGTG 1230 0.0 10.215933 6 TGGCGAG 1190 0.0 10.209064 18 TTAGCGC 75 0.00260248 10.154888 4 AATATCG 85 6.469021E-4 10.08022 5 CCGATCC 95 1.6652071E-4 9.987876 9 TAGGACG 1375 0.0 9.970254 4 GTAGGAC 1310 0.0 9.810902 3 GTCCTAT 305 0.0 9.680095 1 GACGTGA 675 0.0 9.420934 7 GGTATCA 1655 0.0 9.265004 1 ATTATAC 360 0.0 9.255757 3 >>END_MODULE