FastQCFastQC Report
Thu 26 May 2016
SRR1513118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591565
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16840.1058078055247508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT1155.120455E-912.423834
ATAGGAC1551.8189894E-1212.2906283
GTAGGAC7150.011.5901483
GTACTAA1251.704393E-811.455581
ACTGCGG855.3893436E-511.16284758
CTTACAC1408.00901E-910.8859853
TGGCGAG6900.010.86628518
GGCGAGG3500.010.84663619
TAGGACC4350.010.72923854
GGACGTG5500.010.6957016
TGTAGGA7950.010.66986752
TAGGACT2500.010.6679284
TATGGCG6950.010.65155316
TCGAACT1451.4646503E-810.47261319
AGGACGT5850.010.2421155
TAGGACG6000.010.0011834
GTATTAC1053.890729E-510.0009041
ATGGCGA7500.09.87043917
AGACTAT1451.6460581E-79.8152986
TTATACA3250.09.67147354
Produced by FastQC (version 0.11.5)