##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513118_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1591565
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.90038861120972	31.0	30.0	34.0	22.0	34.0
2	28.76719455378825	31.0	30.0	34.0	16.0	34.0
3	28.958109785022916	31.0	30.0	34.0	18.0	34.0
4	32.022737368564904	35.0	33.0	37.0	19.0	37.0
5	32.16753635572534	35.0	33.0	37.0	19.0	37.0
6	32.49833717127481	36.0	33.0	37.0	24.0	37.0
7	32.44508392682674	36.0	33.0	37.0	23.0	37.0
8	32.33112439642741	36.0	33.0	37.0	22.0	37.0
9	33.49905093414344	38.0	34.0	39.0	18.0	39.0
10	33.08393436648833	38.0	33.0	39.0	17.0	39.0
11	33.50087304005805	38.0	33.0	39.0	19.0	39.0
12	33.47585112766365	38.0	34.0	39.0	18.0	39.0
13	33.50507456497221	38.0	34.0	39.0	18.0	39.0
14	33.708257595511334	39.0	33.0	41.0	10.0	41.0
15	34.09123849795641	39.0	33.0	41.0	17.0	41.0
16	33.90974795248702	39.0	33.0	41.0	11.0	41.0
17	33.538392085777204	39.0	32.0	41.0	10.0	41.0
18	33.25893004684069	38.0	32.0	41.0	10.0	41.0
19	33.88237803671229	39.0	33.0	41.0	12.0	41.0
20	34.08152730174388	39.0	34.0	41.0	15.0	41.0
21	34.12291738006302	39.0	34.0	41.0	10.0	41.0
22	33.81988106046564	39.0	34.0	41.0	8.0	41.0
23	33.32806325849086	39.0	33.0	41.0	2.0	41.0
24	33.55301291496106	39.0	33.0	41.0	2.0	41.0
25	33.16584933697336	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0821436699605727
1101	2	1.231216102613729
1101	3	1.1604908973179846
1101	4	1.613157993246432
1101	5	1.543038257570693
1101	6	1.2261917488329601
1101	7	1.2439902528778646
1101	8	1.18572852219544
1101	9	1.7169218744542576
1101	10	1.9842024119860042
1101	11	1.530765117476001
1101	12	1.4296489602929299
1101	13	1.3118853159807315
1101	14	2.251049553772596
1101	15	1.9231726124020767
1101	16	1.9797418375965066
1101	17	2.2924263325573193
1101	18	2.3532136407772413
1101	19	1.845395031888387
1101	20	1.4370740680487515
1101	21	1.3613786975239677
1101	22	1.6404600014323165
1101	23	2.0179871699044014
1101	24	1.6804864048088248
1101	25	1.893444010175422
1102	1	0.8701510376167363
1102	2	1.0787388877200286
1102	3	0.9223642620117865
1102	4	1.2231957817938977
1102	5	1.2303856658728805
1102	6	0.8285836484861377
1102	7	0.9848293232999765
1102	8	1.0275239884047735
1102	9	1.390008769742721
1102	10	1.4329560679657618
1102	11	1.087294042240103
1102	12	0.9947573399580278
1102	13	0.9731561715363597
1102	14	2.0571276864000936
1102	15	1.5296094080628464
1102	16	1.7305114450294923
1102	17	2.1630235626218877
1102	18	2.253922800228814
1102	19	1.5506248218961574
1102	20	1.373106091867875
1102	21	1.3586863866842265
1102	22	1.581607098534704
1102	23	1.8864943143564545
1102	24	1.7075982304848267
1102	25	1.904719617064309
1103	1	0.7859787994577481
1103	2	1.091529161264127
1103	3	0.9098547461074808
1103	4	1.2809130991014257
1103	5	1.1885175165131443
1103	6	1.0484820800854777
1103	7	0.9718727241398852
1103	8	0.7370130144901168
1103	9	1.2592863161338528
1103	10	1.584230546314572
1103	11	1.1166051065693665
1103	12	1.009082632612106
1103	13	1.0869438499018713
1103	14	1.7650617343015327
1103	15	1.4761570122005665
1103	16	1.5208834538919902
1103	17	1.9240171452806436
1103	18	2.189963615204377
1103	19	1.6693443874270315
1103	20	1.3083615172847232
1103	21	1.3167400642849785
1103	22	1.423965675605757
1103	23	1.9316189529516876
1103	24	1.521931107587001
1103	25	1.7436460650493828
1104	1	0.9740678724084759
1104	2	1.2570952890697917
1104	3	1.1495402501144163
1104	4	1.6155924286823122
1104	5	1.5124067970620132
1104	6	1.287656682546924
1104	7	1.239427552316414
1104	8	1.3491208029884376
1104	9	1.7137006178092378
1104	10	1.7814765342590846
1104	11	1.5523063383491902
1104	12	1.370239315032535
1104	13	1.3141326657665502
1104	14	2.1972325752854545
1104	15	1.9281064988543548
1104	16	2.019268159286966
1104	17	2.5937758702501057
1104	18	2.6613048961153183
1104	19	1.8660066294737874
1104	20	1.662831881030769
1104	21	1.5760276366090125
1104	22	1.9349691371165179
1104	23	2.406498883655395
1104	24	2.1226094355167433
1104	25	2.383664472701575
1105	1	0.7597302039102338
1105	2	1.0025947554096746
1105	3	0.9184673956257434
1105	4	1.3012903375252591
1105	5	0.9860110452600921
1105	6	0.6539287042622774
1105	7	0.8053490713371119
1105	8	0.826383587771879
1105	9	1.1064803940672903
1105	10	1.5145996977435559
1105	11	0.9271518404508896
1105	12	0.8822084549832567
1105	13	0.7687030364297343
1105	14	1.599619551343217
1105	15	1.2389450699629165
1105	16	1.4921055181305647
1105	17	1.6826283033449414
1105	18	1.3815596035246287
1105	19	1.1870012138118469
1105	20	1.088275609536126
1105	21	1.0940422892028607
1105	22	1.228304315200873
1105	23	1.5005627383944287
1105	24	1.4019126467012057
1105	25	1.6642682333582997
1106	1	0.862346055099124
1106	2	0.9456367690618883
1106	3	0.8514154638670561
1106	4	1.2686494934983514
1106	5	1.1808420719421662
1106	6	0.8778739175904775
1106	7	0.8580399998259836
1106	8	0.9264260657179264
1106	9	1.1745532783198271
1106	10	0.9945950548052664
1106	11	0.8119195453575827
1106	12	0.9077826481492437
1106	13	0.8597866191975214
1106	14	1.8356047927266204
1106	15	1.4254909214481657
1106	16	1.597276467166914
1106	17	1.79382766321217
1106	18	1.9703963005185727
1106	19	1.5445380856568747
1106	20	1.3409611047993408
1106	21	1.151295151524927
1106	22	1.3931706084567566
1106	23	1.8169182294098505
1106	24	1.3362037095389283
1106	25	1.7921149966376007
1107	1	0.8271355280628789
1107	2	0.9626296678860342
1107	3	0.9963416137317402
1107	4	1.258813491285622
1107	5	1.1665756252597674
1107	6	0.8866939016955655
1107	7	0.828152898778967
1107	8	0.9460331699535871
1107	9	1.097289199769662
1107	10	1.3094735440963277
1107	11	0.9357239848204415
1107	12	0.8752655595105594
1107	13	0.9162486570151671
1107	14	1.813386542889873
1107	15	1.2461902169670722
1107	16	1.3814224619958324
1107	17	1.7657878824556406
1107	18	1.9435688488655956
1107	19	1.326374728819367
1107	20	1.1921159819358706
1107	21	1.005358942130826
1107	22	1.3513004845263836
1107	23	1.5942889987186675
1107	24	1.2980051970899282
1107	25	1.6102347109157193
1108	1	0.7795412518230869
1108	2	0.9565232235481105
1108	3	0.7320121753423159
1108	4	1.2150664871444619
1108	5	1.138389013703808
1108	6	0.7660258153519663
1108	7	0.7089754732137763
1108	8	0.7660121105393216
1108	9	1.0087217899990364
1108	10	1.3849377548790613
1108	11	0.9511484341717633
1108	12	0.7667050647714859
1108	13	0.7207002085753658
1108	14	1.7188698485892786
1108	15	1.301075922496743
1108	16	1.4067605520326865
1108	17	1.683312772640221
1108	18	1.9613420515467368
1108	19	1.1803300482859669
1108	20	1.0109209387562146
1108	21	1.0522334264502007
1108	22	1.2312398208405142
1108	23	1.4966856857899131
1108	24	1.3153304943184168
1108	25	1.5432649877527211
1109	1	0.4591788908891168
1109	2	0.5990764822077708
1109	3	0.5790436443594764
1109	4	0.7570956831186493
1109	5	0.8243880160905022
1109	6	0.46811543638519026
1109	7	0.4134486323646698
1109	8	0.5508783798443986
1109	9	0.7370344344319761
1109	10	1.1404328362666334
1109	11	0.7461802567733926
1109	12	0.6469971507935384
1109	13	0.4942069456221816
1109	14	1.4041320062121798
1109	15	0.9015557871244013
1109	16	1.1816844682331649
1109	17	1.4409817670712712
1109	18	1.6837810897970016
1109	19	1.0100122011102215
1109	20	0.9694764199467869
1109	21	0.834045925660952
1109	22	1.139753313876163
1109	23	1.4622948613067166
1109	24	1.2915844563585992
1109	25	1.58203362986211
1110	1	0.4208693774321475
1110	2	0.6407628412325437
1110	3	0.4723424136658636
1110	4	0.8253197790031024
1110	5	0.7879762157993682
1110	6	0.38765892026266613
1110	7	0.3442507887566535
1110	8	0.44875333809660134
1110	9	0.5695703060315012
1110	10	0.9206106661372573
1110	11	0.46433613832937226
1110	12	0.4925394310740785
1110	13	0.3305878682958081
1110	14	1.1705845775615273
1110	15	0.8459855279414654
1110	16	0.9458434312493438
1110	17	1.3409820821489973
1110	18	0.8774103756418228
1110	19	0.7499155352627582
1110	20	0.5627019336810264
1110	21	0.4247369541376784
1110	22	0.7969265310088289
1110	23	-0.5651778687157787
1110	24	0.1669508917582121
1110	25	0.8326234553720582
1111	1	0.3689678145984452
1111	2	0.8695125742693897
1111	3	0.7020544703499922
1111	4	1.025826223807691
1111	5	0.8185909220142733
1111	6	0.5109208331007693
1111	7	0.48727064453373714
1111	8	0.5987762655203142
1111	9	0.7583620936167677
1111	10	1.0144954825294263
1111	11	0.6694800471724491
1111	12	0.4867033024794978
1111	13	0.5054793556260506
1111	14	1.398348902490234
1111	15	0.9264560740538528
1111	16	1.0839618435409548
1111	17	1.2800125662003055
1111	18	1.5305142148860327
1111	19	1.0554744932898643
1111	20	0.6155573401559806
1111	21	0.5237831104964243
1111	22	0.837506971717886
1111	23	1.2211151437592491
1111	24	0.9052637160399257
1111	25	1.2027055229859798
1112	1	0.07909834662078907
1112	2	0.301608095057631
1112	3	0.018763393731354938
1112	4	0.4528058478259922
1112	5	0.16389012016734483
1112	6	0.10844366203321698
1112	7	0.0017319744865318398
1112	8	0.20246496751771303
1112	9	0.4412009718555936
1112	10	0.7680293291272449
1112	11	0.14046978188272874
1112	12	0.13592619118973914
1112	13	-0.03300388597929782
1112	14	0.6052277287842145
1112	15	0.4953267891199147
1112	16	0.41749228898856927
1112	17	0.9128339233623066
1112	18	0.9807169955839896
1112	19	0.42675770603696606
1112	20	0.3008594756660514
1112	21	0.19226630056918736
1112	22	0.5038193519046388
1112	23	0.7585625805287748
1112	24	0.5066940027671976
1112	25	0.8439627633133142
1113	1	-0.2362238617892558
1113	2	-0.14647035112469808
1113	3	-0.17460885847553342
1113	4	0.23309648054616616
1113	5	0.20568070305877484
1113	6	-0.15723796899278142
1113	7	-0.13536985449071892
1113	8	-0.2181401370439744
1113	9	-0.14569970633986884
1113	10	0.1420924175654008
1113	11	-0.12394973944659426
1113	12	-0.3007493397486627
1113	13	-0.3657700895008773
1113	14	0.2470705007311551
1113	15	0.03588607746051764
1113	16	0.16675600369539012
1113	17	0.5492053678189848
1113	18	0.5741898231817686
1113	19	0.23837291796649396
1113	20	0.025878882651511503
1113	21	0.034698625263793303
1113	22	0.06827545802413226
1113	23	0.24482951574616152
1113	24	0.19392223940653253
1113	25	0.5554635002542483
1114	1	-0.9086300090078545
1114	2	-0.46118930751238807
1114	3	-0.5040260499927527
1114	4	-0.27845265127270835
1114	5	-3.2361275368749176
1114	6	-1.213139832058264
1114	7	-1.0359183739106292
1114	8	-0.49428323778888483
1114	9	-0.17642260108643626
1114	10	0.003677052686484217
1114	11	-0.32017741460062155
1114	12	-0.6339682882174031
1114	13	-0.5794484955741837
1114	14	-5.872267448471405
1114	15	-2.348972358071297
1114	16	-1.25484834961658
1114	17	-0.2507414432189776
1114	18	-6.446496459609289
1114	19	-2.4982156766306716
1114	20	-1.4098931359949773
1114	21	-1.0326962770427315
1114	22	-0.7635622932046218
1114	23	-0.21724068312512657
1114	24	-0.36247653790724854
1114	25	-0.19026554487849978
1115	1	-1.4142293534482206
1115	2	-0.9895415111561761
1115	3	-1.1719185363022824
1115	4	-3.893091834197591
1115	5	-1.7920348401422075
1115	6	-1.4835853028426023
1115	7	-1.3448559688364554
1115	8	-1.2041687604830642
1115	9	-1.1377709879102937
1115	10	-1.35399049779393
1115	11	-1.3856013518066277
1115	12	-1.4800973614138293
1115	13	-1.4691888644834101
1115	14	-7.197162646265099
1115	15	-3.5358286876196487
1115	16	-2.215882022783802
1115	17	-1.2458378803533492
1115	18	-1.068431540919697
1115	19	-1.2906030859010258
1115	20	-1.3700065489033904
1115	21	-1.587849632146181
1115	22	-2.9180907924452164
1115	23	-2.014680227399282
1115	24	-1.7933961226830952
1115	25	-1.3836634834955213
1116	1	-1.0580943250459534
1116	2	-0.8301991412496719
1116	3	-0.8764811652342743
1116	4	-0.9372254409974552
1116	5	-1.6436483390114773
1116	6	-1.1735729333133378
1116	7	-1.1490935749850841
1116	8	-0.9690196411419265
1116	9	-1.0254407711159246
1116	10	-0.8018820192063174
1116	11	-1.159841956027357
1116	12	-1.3793470491973778
1116	13	-1.4236022847277425
1116	14	-0.5788241738523254
1116	15	-1.114588240251699
1116	16	-1.0156604434153067
1116	17	-0.6830866429040512
1116	18	-0.6432024663709512
1116	19	-0.9374583862343897
1116	20	-1.4910909376838504
1116	21	-1.4330277990335674
1116	22	-1.4006692138709056
1116	23	-2.0624492448059684
1116	24	-1.733981084274017
1116	25	-1.1880108865892929
1201	1	1.2226804947357905
1201	2	1.4583941590950928
1201	3	1.374032183738553
1201	4	1.7842269424424089
1201	5	1.7708840432886674
1201	6	1.3560540932516432
1201	7	1.3446626578597503
1201	8	1.4416661854876338
1201	9	1.7641298392992155
1201	10	2.0512640834254867
1201	11	1.4527453038003486
1201	12	1.5374742711684704
1201	13	1.4307376748121925
1201	14	2.4208025677826015
1201	15	2.0985023959971016
1201	16	2.2231831204897574
1201	17	2.5637368619255128
1201	18	1.7350364759687054
1201	19	1.8623763543368028
1201	20	1.7586749447920056
1201	21	1.778856633338286
1201	22	2.0901132990221782
1201	23	2.2629256756510614
1201	24	2.0011004532085295
1201	25	2.4460040627406485
1202	1	0.9251964082148518
1202	2	1.1810213037736474
1202	3	1.1973937599489552
1202	4	1.6024449511365262
1202	5	1.2984809368374783
1202	6	0.932342014606661
1202	7	1.0590092223151117
1202	8	1.1117636480855921
1202	9	1.2781410229055084
1202	10	1.6418742155244743
1202	11	1.231913330083259
1202	12	1.1866509406313028
1202	13	1.1814799854859643
1202	14	2.0153621396666423
1202	15	1.7029702674031384
1202	16	1.754425271193071
1202	17	2.32625952440695
1202	18	2.3337965059571957
1202	19	1.9774872751458545
1202	20	1.6445843330681598
1202	21	1.50048145565966
1202	22	1.8146242749056327
1202	23	2.0015220104970837
1202	24	1.6076027349761972
1202	25	2.050946911106159
1203	1	0.5064045269982387
1203	2	0.6235512889973549
1203	3	0.5702650325167156
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2211	16	-6.917719098496512
2211	17	-2.06419009092426
2211	18	-0.7238909212582882
2211	19	-0.659147569537538
2211	20	-0.7478862417186818
2211	21	-0.742760398143524
2211	22	-0.6326298015708787
2211	23	-0.10153496248089766
2211	24	-0.5745280819219545
2211	25	-7.842253955054822
2212	1	-1.5127562511618464
2212	2	-4.001861479006628
2212	3	-1.802955149883342
2212	4	-3.848159429070794
2212	5	-1.4944583247679688
2212	6	-1.1493105175995773
2212	7	-1.0397985011993356
2212	8	-0.9294583849445424
2212	9	-1.1201735869229452
2212	10	-0.4258985431343234
2212	11	-1.0249453580768204
2212	12	-1.1105210276536184
2212	13	-1.2510372509039627
2212	14	-6.384668019818424
2212	15	-5.000161495968229
2212	16	-2.883600161677961
2212	17	-7.561360373282795
2212	18	-2.977567209495998
2212	19	-1.9731870091311023
2212	20	-1.990380583636103
2212	21	-1.5946271520649518
2212	22	-1.4715935119864696
2212	23	-0.7427713448311053
2212	24	-1.0600150136976083
2212	25	-0.9275491928866728
2213	1	-0.15911748341544651
2213	2	-0.09815024206332623
2213	3	-0.10014256637564145
2213	4	0.18616389385866938
2213	5	0.1503974830615391
2213	6	-0.23229851372747135
2213	7	-0.309355893440852
2213	8	-0.21626533372884893
2213	9	0.039349195894217814
2213	10	-3.7318893300784204
2213	11	-1.4868605387366038
2213	12	-0.859676438539509
2213	13	-0.8020199355735826
2213	14	-0.05815550184860285
2213	15	-0.5704294815723117
2213	16	-0.27461262449214985
2213	17	0.07307249404932037
2213	18	0.31795934396102155
2213	19	-0.3141181542505933
2213	20	-0.7107117180194251
2213	21	-0.7227834849643173
2213	22	-1.0967569879831487
2213	23	-1.2562408231177784
2213	24	-0.737609968402154
2213	25	-0.4140794196500792
2214	1	-0.961839976988788
2214	2	-0.8039828764919363
2214	3	-0.7419027022747713
2214	4	-0.27615104477719754
2214	5	-3.5903504132500856
2214	6	-1.449748695620766
2214	7	-1.12453699434489
2214	8	-0.9187562835804286
2214	9	-0.8494332497559256
2214	10	-4.461610854385171
2214	11	-2.0976430211868227
2214	12	-1.2952866877175921
2214	13	-0.9500150138598187
2214	14	-0.3659388965846446
2214	15	-0.5386481618858738
2214	16	-0.39098070600313406
2214	17	-0.1044107248179671
2214	18	0.11540696171979192
2214	19	-0.564112663916255
2214	20	-0.8203373721853424
2214	21	-0.7961346485293888
2214	22	-0.7130072498869353
2214	23	-0.4386700912888557
2214	24	-1.0606918088097075
2214	25	-0.5819996631953757
2215	1	-2.2265368153864635
2215	2	-4.731179365464065
2215	3	-3.01332807895551
2215	4	-2.031092853776535
2215	5	-2.1895742226407826
2215	6	-2.184865496932744
2215	7	-2.2827447674326002
2215	8	-2.5327798954292184
2215	9	-8.630237208048488
2215	10	-4.356205474163588
2215	11	-3.25625985511185
2215	12	-3.646516199733174
2215	13	-3.004810530031232
2215	14	-7.664975413883539
2215	15	-4.694814357992804
2215	16	-9.510749805603393
2215	17	-4.596017989142084
2215	18	-2.963503576022827
2215	19	-3.4735629942398702
2215	20	-3.269447967699648
2215	21	-3.5884780360003887
2215	22	-9.508971617799418
2215	23	-10.801469781930773
2215	24	-7.1934492888458585
2215	25	-5.37287546337987
2216	1	-2.7640298935166676
2216	2	-2.766972745121034
2216	3	-2.5279779749204963
2216	4	-7.281521411801581
2216	5	-4.367349363515778
2216	6	-3.658843865744686
2216	7	-3.078450540593959
2216	8	-8.58194090042032
2216	9	-5.128666472518187
2216	10	-3.2409896865973806
2216	11	-3.105837184125569
2216	12	-3.3912950402440956
2216	13	-3.1744560383126377
2216	14	-2.676421289001052
2216	15	-3.1900839383757216
2216	16	-2.793015837205754
2216	17	-2.562176898715329
2216	18	-8.181939757763072
2216	19	-5.870061677934796
2216	20	-5.142848486862878
2216	21	-4.343631494543679
2216	22	-4.0161707303283265
2216	23	-3.327687525097666
2216	24	-3.6065773852902723
2216	25	-3.138249373127884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87895.0
3	2.0
4	756.0
5	4183.0
6	5123.0
7	4576.0
8	4201.0
9	3922.0
10	3744.0
11	3558.0
12	3516.0
13	3429.0
14	3500.0
15	3492.0
16	3558.0
17	3808.0
18	4254.0
19	5239.0
20	6815.0
21	8606.0
22	10646.0
23	12750.0
24	14533.0
25	16669.0
26	18858.0
27	21033.0
28	23962.0
29	27730.0
30	33305.0
31	39754.0
32	50194.0
33	64366.0
34	82713.0
35	108563.0
36	151931.0
37	215605.0
38	534776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.542272611974916	18.44966958737288	12.375981182939517	25.632076617712684
2	14.719240180255646	20.20614141439589	40.842893537690244	24.23172486765822
3	18.334722198602066	24.79278823048487	29.8269681732548	27.045521397658266
4	11.620009525942422	16.67872655182964	38.83644713890527	32.86481678332266
5	12.866446639121234	37.81649954650811	36.23626927340522	13.080784540965434
6	31.82165244850827	37.23555010918918	18.095300937927135	12.847496504375421
7	27.880644212569035	31.505219710471344	22.58948164868948	18.024654428270143
8	25.33273507318946	35.97340758797472	20.91974956025994	17.774107778575875
9	26.815105660820816	14.567851736127913	19.81931424628489	38.797728356766385
10	14.170610156922633	28.502793436523948	35.58140557933171	21.74519082722171
11	34.606547816556564	22.25982373583502	24.344162569253818	18.7894658783546
12	22.893397186922584	24.679600789371143	31.693628467639556	20.73337355606672
13	28.968181143990567	21.514054145099212	25.658901964136227	23.858862746773994
14	21.016081228176464	21.018532251698748	28.48397282003454	29.481413700090247
15	23.63196479002937	29.5155674760342	23.438616693684697	23.41385104025173
16	22.77759657725835	27.48606588953639	27.097877817222933	22.638459715982325
17	22.114102101543377	27.624289250411394	27.822161433027055	22.43944721501817
18	22.09328781277576	27.114543868426143	28.91885742730077	21.873310891497326
19	23.705816769924798	26.838566628832588	27.162041733127694	22.29357486811492
20	23.859539743273885	26.901311786976656	27.300215195410505	21.938933274338954
21	23.67892757970054	26.449543625527806	26.739986599774486	23.131542194997166
22	23.628597828888232	27.021415685559656	26.807070256271647	22.542916229280465
23	22.956939711573874	26.755338070463665	27.305014461529563	22.982707756432898
24	23.29257261465563	26.759884311890215	27.22467342498785	22.722869648466304
25	23.329633349633376	26.632088789552217	27.17426420397763	22.86401365683678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	274.5
3	384.0
4	384.0
5	384.0
6	721.0
7	1058.0
8	1058.0
9	1058.0
10	1664.0
11	2270.0
12	2270.0
13	2270.0
14	4154.0
15	6038.0
16	6038.0
17	6038.0
18	11438.0
19	16838.0
20	16838.0
21	16838.0
22	26852.5
23	36867.0
24	36867.0
25	36867.0
26	52984.5
27	69102.0
28	69102.0
29	69102.0
30	88684.0
31	108266.0
32	108266.0
33	108266.0
34	126384.0
35	144502.0
36	144502.0
37	144502.0
38	159483.5
39	174465.0
40	174465.0
41	174465.0
42	185862.5
43	197260.0
44	197260.0
45	197260.0
46	203756.0
47	210252.0
48	210252.0
49	210252.0
50	204452.0
51	198652.0
52	198652.0
53	198652.0
54	179978.5
55	161305.0
56	161305.0
57	161305.0
58	138859.0
59	116413.0
60	116413.0
61	116413.0
62	95295.0
63	74177.0
64	74177.0
65	74177.0
66	57643.5
67	41110.0
68	41110.0
69	41110.0
70	30351.0
71	19592.0
72	19592.0
73	19592.0
74	13862.5
75	8133.0
76	8133.0
77	8133.0
78	5621.0
79	3109.0
80	3109.0
81	3109.0
82	2139.5
83	1170.0
84	1170.0
85	1170.0
86	748.0
87	326.0
88	326.0
89	326.0
90	208.0
91	90.0
92	90.0
93	90.0
94	50.5
95	11.0
96	11.0
97	11.0
98	10.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17555048018773972
2	0.24001533082217819
3	0.24742941695752294
4	0.40337655075350365
5	0.2440993613204613
6	0.0182210591461863
7	0.018158227907751175
8	0.01784407171557555
9	0.018472384099926804
10	0.021048464875766934
11	0.019917502583934682
12	0.0267032763349282
13	0.027205926242409202
14	0.024692676705004194
15	0.040965967459701616
16	0.020482983729850808
17	0.04014916136004499
18	0.03072447559477621
19	0.025572314043095946
20	0.0284625510111117
21	0.03436868742401347
22	0.042411085943709494
23	0.028085563580500954
24	0.04624379148825213
25	0.035625312192715974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1591565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.67628681340665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.97224881480265	65.15356521303961
2	10.515185161658632	16.1253070670283
3	2.5134459334531654	5.781651038503364
4	0.8373726629688029	2.568265059020081
5	0.3802298737361343	1.4577307426808617
6	0.20471152176780003	0.9417911614246063
7	0.12917307564614017	0.693315825776932
8	0.08514719517812024	0.522301660706773
9	0.060179112965068476	0.41528798332973904
>10	0.28094515995558994	3.6106332365472893
>50	0.012824661683000168	0.687836784685464
>100	0.007459478486437678	1.2396281574698478
>500	6.629831989721276E-4	0.3844403674962288
>1k	4.143644993575797E-4	0.4182457022909385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1684	0.1058078055247508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.283123843512518E-5
7	0.0	0.0	0.0	0.0	6.283123843512518E-5
8	0.0	0.0	0.0	0.0	6.283123843512518E-5
9	0.0	0.0	0.0	0.0	6.283123843512518E-5
10	0.0	0.0	0.0	0.0	6.283123843512518E-5
11	0.0	0.0	0.0	0.0	6.283123843512518E-5
12	0.0	0.0	0.0	0.0	1.8849371530537553E-4
13	0.0	0.0	6.283123843512518E-5	0.0	2.5132495374050073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGAGT	115	5.120455E-9	12.42383	4
ATAGGAC	155	1.8189894E-12	12.290628	3
GTAGGAC	715	0.0	11.590148	3
GTACTAA	125	1.704393E-8	11.45558	1
ACTGCGG	85	5.3893436E-5	11.1628475	8
CTTACAC	140	8.00901E-9	10.885985	3
TGGCGAG	690	0.0	10.866285	18
GGCGAGG	350	0.0	10.846636	19
TAGGACC	435	0.0	10.7292385	4
GGACGTG	550	0.0	10.695701	6
TGTAGGA	795	0.0	10.6698675	2
TAGGACT	250	0.0	10.667928	4
TATGGCG	695	0.0	10.651553	16
TCGAACT	145	1.4646503E-8	10.472613	19
AGGACGT	585	0.0	10.242115	5
TAGGACG	600	0.0	10.001183	4
GTATTAC	105	3.890729E-5	10.000904	1
ATGGCGA	750	0.0	9.870439	17
AGACTAT	145	1.6460581E-7	9.815298	6
TTATACA	325	0.0	9.6714735	4
>>END_MODULE