FastQCFastQC Report
Thu 26 May 2016
SRR1513118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591565
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22270.13992516799502377No Hit
TATCAACGCAGAGTACTTTTTTTTT17930.11265641051417943No Hit
CCCATGTACTCTGCGTTGATACCAC16190.10172377502646766No Hit
GTACATGGGAAGCAGTGGTATCAAC15960.10027865654245978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3150.012.0893734
CTAGTAC1001.8796509E-611.4248183
CCTAATA1604.3655746E-1111.3065222
GGCGTGT600.005925395511.0708148
CAATACT1502.057277E-910.7897654
CGTGCAC803.813684E-410.67442110
GTAGGAC6900.010.6245293
TGCGCCA909.605738E-510.5456212
TGGCGAG7400.010.51612818
CTAATAC1553.5306584E-910.4420383
GGCGAGG3500.010.3013419
GCCGTGC856.6619803E-410.04746158
GTCCTAA4300.09.9682181
TGTAGGA7850.09.9457982
TAGGACG6350.09.89524
GTATAAT3000.09.8426911
AGGACGT6300.09.8226165
TTAGGAC4850.09.8151363
TTAGAGT1659.860742E-99.8088784
GACGTGA3400.09.7702077
Produced by FastQC (version 0.11.5)