FastQCFastQC Report
Thu 26 May 2016
SRR1513117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451914
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGT655.5292727E-513.1348966
ATACCGT604.1436075E-412.6484186
TAGGACC6100.012.0294894
CGCAGAT2050.011.1066078
GGACGTG12300.011.1059286
TTATACT3150.010.8912634
TGGACCG1053.4207224E-610.8696585
AGGACGT12650.010.8266955
GGCGAGG6500.010.51220519
GTAGGAC14600.010.4436763
GTCCTAC13400.010.4090821
TGGCGAG14150.010.39539718
TAGGACG13650.010.2629194
TTAGGAC9400.010.2395253
ATTGGAC2053.6379788E-1210.227163
TATGGCG14800.010.13038116
TGTAGGA15750.010.05137352
ATGGCGA15450.010.01127817
AGGACCT12000.09.9864995
GTGCGCC1252.610208E-69.86838311
Produced by FastQC (version 0.11.5)