##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513117_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2451914
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.356710716607516	31.0	30.0	34.0	25.0	34.0
2	29.22107626939607	31.0	30.0	34.0	22.0	34.0
3	29.41079744232465	31.0	30.0	34.0	23.0	34.0
4	32.470561365529136	37.0	33.0	37.0	22.0	37.0
5	32.635590807834205	37.0	33.0	37.0	24.0	37.0
6	32.98902041425597	37.0	35.0	37.0	26.0	37.0
7	32.95108596794178	37.0	35.0	37.0	26.0	37.0
8	32.82516964298095	37.0	35.0	37.0	25.0	37.0
9	34.07152412360303	39.0	35.0	39.0	23.0	39.0
10	33.656797098103766	39.0	34.0	39.0	18.0	39.0
11	34.09507388921471	39.0	35.0	39.0	25.0	39.0
12	34.08130260686141	39.0	35.0	39.0	25.0	39.0
13	34.11701470769366	39.0	35.0	39.0	25.0	39.0
14	34.34965663559162	39.0	34.0	41.0	17.0	41.0
15	34.77085819486328	40.0	34.0	41.0	19.0	41.0
16	34.6168209814863	39.0	34.0	41.0	17.0	41.0
17	34.230238091548074	39.0	33.0	41.0	17.0	41.0
18	33.96012747592289	39.0	33.0	41.0	16.0	41.0
19	34.591169592408214	39.0	34.0	41.0	18.0	41.0
20	34.79927069220209	40.0	34.0	41.0	19.0	41.0
21	34.84476698611778	40.0	35.0	41.0	19.0	41.0
22	34.5361370749545	40.0	34.0	41.0	17.0	41.0
23	34.07547532254394	39.0	34.0	41.0	10.0	41.0
24	34.31685532200559	39.0	34.0	41.0	10.0	41.0
25	33.96007771887595	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9392129216428486
1101	2	1.2134309856761298
1101	3	1.1414490822026053
1101	4	1.4972306711557053
1101	5	1.442339195951007
1101	6	1.1744776874885332
1101	7	1.081975453197849
1101	8	1.081401402841287
1101	9	1.490833489470056
1101	10	1.8449848544857517
1101	11	1.3656760099229857
1101	12	1.2222762830089025
1101	13	1.1700211075023503
1101	14	2.155312570000298
1101	15	1.800138804085762
1101	16	1.8213925255512677
1101	17	2.2003479519832467
1101	18	2.2660591574711333
1101	19	1.8079846012795144
1101	20	1.4655808495873899
1101	21	1.4159177938940672
1101	22	1.6376277708201172
1101	23	1.9635360274883595
1101	24	1.5872771453501215
1101	25	1.868537814738346
1102	1	1.1535897924369038
1102	2	1.3433083502528085
1102	3	1.2556756779765585
1102	4	1.6126663464149047
1102	5	1.5307871286375843
1102	6	1.3302064831175642
1102	7	1.2934423697623458
1102	8	1.2936230577315655
1102	9	1.5652278796103047
1102	10	1.8410426958454735
1102	11	1.424244132050866
1102	12	1.2683871716035924
1102	13	1.2719647459617462
1102	14	2.2852808945331944
1102	15	1.8761622182486093
1102	16	1.9851817491660029
1102	17	2.2815207697851037
1102	18	2.499793682813035
1102	19	1.895058044947021
1102	20	1.641282311367398
1102	21	1.634428680042781
1102	22	1.8233840531720489
1102	23	2.16751319424624
1102	24	1.8597119981478656
1102	25	2.234184914568047
1103	1	0.6671718201549588
1103	2	0.965494586784633
1103	3	0.7532904919849699
1103	4	1.2123869281130055
1103	5	1.086987100211914
1103	6	0.8049068795649603
1103	7	0.7526746649538723
1103	8	0.7353740186099813
1103	9	1.1015025139321892
1103	10	1.5024002635756801
1103	11	1.0526247457429676
1103	12	0.9409798347563623
1103	13	1.0788625072889246
1103	14	1.9102575340745105
1103	15	1.4475658416145123
1103	16	1.4692979980660184
1103	17	1.8015988194211658
1103	18	2.0696880239876165
1103	19	1.412629494686506
1103	20	1.1311332882730483
1103	21	1.2133259454327074
1103	22	1.4749526059122573
1103	23	1.8879975193028642
1103	24	1.554006149414775
1103	25	1.801018204053264
1104	1	0.775897453588712
1104	2	1.059560414763034
1104	3	0.8630763377197717
1104	4	1.2237001691302964
1104	5	1.155232879834287
1104	6	0.9970380355842536
1104	7	0.9438727479521134
1104	8	0.9803390876315277
1104	9	1.2987450999882384
1104	10	1.459534932086946
1104	11	1.0652934207792768
1104	12	0.9415530389679105
1104	13	0.9515616095563146
1104	14	1.9377131538461754
1104	15	1.4002894235805599
1104	16	1.4517333832980412
1104	17	1.9189806984104294
1104	18	2.0712093855415574
1104	19	1.4251085382543351
1104	20	1.1227765742416125
1104	21	1.1566476853876253
1104	22	1.4084760016946802
1104	23	1.6885073693647144
1104	24	1.514364552890207
1104	25	1.772526075914115
1105	1	0.8066299797162557
1105	2	1.1404269781622105
1105	3	1.02625279981255
1105	4	1.332136831304382
1105	5	1.303460172123735
1105	6	0.883658500350414
1105	7	0.9069493783621212
1105	8	0.9725256818518062
1105	9	1.2246682660328574
1105	10	1.5981569202084316
1105	11	1.1993112823510188
1105	12	1.1483738096396436
1105	13	1.0492017399889662
1105	14	1.7957744246490606
1105	15	1.4508087617672984
1105	16	1.6230874315568116
1105	17	1.9307089131984725
1105	18	1.568003936266635
1105	19	1.4433638145008132
1105	20	1.3450409879427951
1105	21	1.279167501314781
1105	22	1.34027528557489
1105	23	1.714640825970541
1105	24	1.5238207490993219
1105	25	1.6984370455970037
1106	1	0.6963682202952448
1106	2	0.8919878638279712
1106	3	0.7994487290926386
1106	4	1.2668351731593859
1106	5	1.2166491107743553
1106	6	0.8762487268335875
1106	7	0.8427076964401721
1106	8	0.8630867165824583
1106	9	1.047298740037185
1106	10	0.9840647804810203
1106	11	0.8982017402152209
1106	12	0.8710199978740931
1106	13	0.7847741308209564
1106	14	1.7102857084208907
1106	15	1.3560705322029634
1106	16	1.500855026905107
1106	17	1.7815852018204197
1106	18	1.795183459596764
1106	19	1.3251374370640931
1106	20	1.0463028259349585
1106	21	0.9066725441874581
1106	22	1.1734981052805296
1106	23	1.6030396854405282
1106	24	1.3621137578089701
1106	25	1.5600717808012163
1107	1	0.7500748910823987
1107	2	0.9088457409050328
1107	3	0.8002841389163962
1107	4	1.0404498694406215
1107	5	0.9504175744506327
1107	6	0.7907397297513867
1107	7	0.7509544261031422
1107	8	0.8352391207504084
1107	9	1.157161774704889
1107	10	1.2433244355296935
1107	11	0.8194260596666965
1107	12	0.7600364165292177
1107	13	0.6851284398966015
1107	14	1.6554493931486292
1107	15	1.0329366839529541
1107	16	1.3067494677834333
1107	17	1.6150940891512846
1107	18	1.7737249798982333
1107	19	1.2184065754944626
1107	20	0.9976703492156034
1107	21	0.8417608398575567
1107	22	1.198958970626478
1107	23	1.52159047684151
1107	24	1.316480958334047
1107	25	1.5048430323057786
1108	1	0.6316021162378256
1108	2	0.8589378022838012
1108	3	0.7091052620482792
1108	4	1.164385771458626
1108	5	1.0926128812117994
1108	6	0.7204736918461521
1108	7	0.640285554028651
1108	8	0.6595357023682809
1108	9	0.9556375533307246
1108	10	1.0923353050396756
1108	11	0.7748895679589936
1108	12	0.4822398651185438
1108	13	0.5759114895363453
1108	14	1.5244616629175454
1108	15	1.0360473196535693
1108	16	1.0329243160840136
1108	17	1.4403379628772939
1108	18	1.6988873506495352
1108	19	1.0380503309889306
1108	20	0.8830309243606678
1108	21	0.8066367136784152
1108	22	1.046014559212331
1108	23	1.359424541640756
1108	24	1.0013783752515408
1108	25	1.4022461166019369
1109	1	0.25658376623691836
1109	2	0.4381034174335632
1109	3	0.4259824795118696
1109	4	0.5570065144645966
1109	5	0.6324398056526803
1109	6	0.348134029618123
1109	7	0.3667266270212437
1109	8	0.3683248992073018
1109	9	0.5992883705449259
1109	10	0.8894444995034974
1109	11	0.5212098773276423
1109	12	0.42501961397506705
1109	13	0.35330453583442534
1109	14	1.2333168372879157
1109	15	0.7959752886837137
1109	16	0.8981685071747378
1109	17	1.1642553952956831
1109	18	1.3705996490263246
1109	19	0.720737785526282
1109	20	0.656498719902892
1109	21	0.5189806561987922
1109	22	0.8136532398596401
1109	23	1.2674992842871475
1109	24	0.9274563244059593
1109	25	1.0514147172349908
1110	1	0.32937216857481033
1110	2	0.5704673245821823
1110	3	0.4884308655606979
1110	4	0.7585515579944584
1110	5	0.6780850830390719
1110	6	0.4058140519907596
1110	7	0.35814421624073134
1110	8	0.3790471763725307
1110	9	0.5902569179611277
1110	10	0.8955993722132334
1110	11	0.49419387586407737
1110	12	0.44622964738813664
1110	13	0.238608807204173
1110	14	1.122420909164191
1110	15	0.7983116622273201
1110	16	0.9915480378411203
1110	17	1.278166533648843
1110	18	0.869505708946825
1110	19	0.7175894244021563
1110	20	0.5859102525375022
1110	21	0.5064559104406285
1110	22	0.5778260190359319
1110	23	-0.6015188655359651
1110	24	0.2186885640183931
1110	25	0.6358459803801324
1111	1	0.2704304844552716
1111	2	0.7071083834274958
1111	3	0.6361705904818571
1111	4	1.0905602356086064
1111	5	0.8155480369435892
1111	6	0.5785030903675121
1111	7	0.5328093144442576
1111	8	0.5634216136401378
1111	9	0.8089874935363
1111	10	1.0037572503193033
1111	11	0.5959257060623244
1111	12	0.46065456244256353
1111	13	0.3448641430046635
1111	14	1.257243531892712
1111	15	0.9104778201353128
1111	16	1.0601876681545477
1111	17	1.3602703249748913
1111	18	1.6538648127514861
1111	19	1.1719081364609636
1111	20	0.805206182541852
1111	21	0.6633158960199381
1111	22	1.0095326984679787
1111	23	1.2677635639171996
1111	24	1.0417553464098148
1111	25	1.2727730337757635
1112	1	-0.09242381501290708
1112	2	0.09529326429845497
1112	3	0.004090119291358718
1112	4	0.31519412624214027
1112	5	0.009774425361293027
1112	6	-0.18218901250696007
1112	7	-0.22981651754002286
1112	8	-0.12590897098787934
1112	9	0.051411167879628294
1112	10	0.3443967456025021
1112	11	-0.13103327561854883
1112	12	-0.09351797263740025
1112	13	-0.2809095854923527
1112	14	0.2647432078575278
1112	15	0.15372070380859526
1112	16	0.05225500458127641
1112	17	0.5169818042464129
1112	18	0.6723780566692525
1112	19	0.15952525527514183
1112	20	-0.055757424527136834
1112	21	-0.12375539232582611
1112	22	0.06957929099861815
1112	23	0.4708440248272723
1112	24	0.18332778082216805
1112	25	0.44354491885020764
1113	1	-0.04003682821563004
1113	2	0.057232038367949656
1113	3	0.06272034459237474
1113	4	0.3157753224805546
1113	5	0.34994713088264007
1113	6	-0.01185104222210498
1113	7	0.006860559639719099
1113	8	0.005884250290058901
1113	9	0.07471951748215844
1113	10	0.4025133683128317
1113	11	0.0796959575291325
1113	12	-0.08716546569404215
1113	13	-0.1901380691417458
1113	14	0.33952722364456633
1113	15	0.12097195662718718
1113	16	0.3390760644155719
1113	17	0.5123328482067677
1113	18	0.42585645238211356
1113	19	0.35453838556288986
1113	20	0.15755100760648588
1113	21	0.01642646664409142
1113	22	0.25456325643487077
1113	23	0.3184202127924536
1113	24	0.12753014542666818
1113	25	0.48591051645274064
1114	1	-1.032698053576187
1114	2	-0.6849492969645503
1114	3	-0.714752126475954
1114	4	-0.41669840085280896
1114	5	-3.336436752382852
1114	6	-1.2707321760218626
1114	7	-1.0730790240064039
1114	8	-0.6859023289732988
1114	9	-0.33507744170402987
1114	10	-0.16455911693334002
1114	11	-0.6763579998791442
1114	12	-0.8099052276332799
1114	13	-0.7439665727000886
1114	14	-6.048693459894853
1114	15	-2.3109635769697974
1114	16	-1.318629979995194
1114	17	-0.4701863345205979
1114	18	-6.303156338109101
1114	19	-2.4230445657097874
1114	20	-1.444772554269953
1114	21	-1.1872211188919692
1114	22	-0.7932235114206065
1114	23	-0.40237524467885777
1114	24	-0.6340244822526984
1114	25	-0.4395262465040233
1115	1	-1.0043960508751084
1115	2	-0.592322008678277
1115	3	-0.5799487218300108
1115	4	-3.228427502261688
1115	5	-1.2980916107130511
1115	6	-0.8753891348025462
1115	7	-0.7450024083321836
1115	8	-0.5791671687562641
1115	9	-0.2911764668518231
1115	10	-0.6954063919975013
1115	11	-0.7794617584409735
1115	12	-0.7723070047563496
1115	13	-0.8513413215229377
1115	14	-6.1195301738213885
1115	15	-2.5926294356892754
1115	16	-1.5042451885426544
1115	17	-0.6541728106623879
1115	18	-0.4788845011745977
1115	19	-0.7412650108372318
1115	20	-0.7248289694608019
1115	21	-1.03065043932191
1115	22	-2.027162131412858
1115	23	-1.2983249942895014
1115	24	-1.0068324493864935
1115	25	-0.6050877708600453
1116	1	-0.4095892221233264
1116	2	-0.15618194868500623
1116	3	-0.260741453081458
1116	4	-0.06894030149008756
1116	5	-0.7395292952700032
1116	6	-0.5288411093554402
1116	7	-0.44409599733403127
1116	8	-0.36761813610630867
1116	9	-0.31598931735275215
1116	10	-0.2284965535280321
1116	11	-0.4646498060143429
1116	12	-0.7535433942496255
1116	13	-0.7068600715371005
1116	14	0.1291790978236591
1116	15	-0.318933304194978
1116	16	-0.289123656907357
1116	17	0.11593263375497997
1116	18	-0.10660694541080318
1116	19	-0.22552545381326894
1116	20	-0.724359239633273
1116	21	-0.7277763749799533
1116	22	-0.8144272351917223
1116	23	-1.436088994438819
1116	24	-1.0622135185290276
1116	25	-0.45319055015549026
1201	1	0.9874729961626016
1201	2	1.19204952382945
1201	3	1.1140209207248937
1201	4	1.5130836160978873
1201	5	1.5290883203839414
1201	6	1.226134670476938
1201	7	1.0822932709290427
1201	8	1.1947307465102597
1201	9	1.4304884807502916
1201	10	1.7429308222970477
1201	11	1.2907320191571614
1201	12	1.365875781574907
1201	13	1.083873909445444
1201	14	2.1223894495426165
1201	15	1.8004858716295544
1201	16	1.9164074807461517
1201	17	2.2110801390363264
1201	18	1.8334629698025182
1201	19	1.653959093931313
1201	20	1.5649092073789603
1201	21	1.3889871815541568
1201	22	1.6749843163613178
1201	23	1.8983295789854893
1201	24	1.5284619698520032
1201	25	1.8953154513141826
1202	1	0.8687625138255832
1202	2	0.9427570654483546
1202	3	0.8976562936555119
1202	4	1.3180159564909104
1202	5	1.1909483542889205
1202	6	0.9119962890791271
1202	7	0.8443277893229677
1202	8	0.8913024355351098
1202	9	1.1361175340473864
1202	10	1.458621262565046
1202	11	1.100571322811028
1202	12	0.9840273062186071
1202	13	1.0362138287806104
1202	14	1.9103885309765545
1202	15	1.5668919685560923
1202	16	1.6041054147669414
1202	17	1.9317497834943893
1202	18	1.9675058283486138
1202	19	1.497500832817252
1202	20	1.234746109931443
1202	21	1.2157099253968013
1202	22	1.4136743975035486
1202	23	1.7266431824276296
1202	24	1.4161219792410975
1202	25	1.8000301853974037
1203	1	0.6550493455003163
1203	2	0.7948005127452156
1203	3	0.6268185822397712
1203	4	1.0338782177463415
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2211	12	-0.3968815375877668
2211	13	-0.4670813317583651
2211	14	0.11421258285714941
2211	15	-0.2392907245523972
2211	16	-6.497933365717067
2211	17	-1.837946820124472
2211	18	-0.5724374112412676
2211	19	-0.6634023916902123
2211	20	-0.7904968057708359
2211	21	-0.7197843669879518
2211	22	-0.5499694707339629
2211	23	-0.222117780467606
2211	24	-0.5870775433930575
2211	25	-7.796976393941332
2212	1	-1.719656902487042
2212	2	-4.226941391245752
2212	3	-2.050970096044672
2212	4	-3.736742496136234
2212	5	-1.437175602800906
2212	6	-1.240532124107169
2212	7	-1.1725065453632446
2212	8	-1.026970844129366
2212	9	-1.1772355212983996
2212	10	-0.5433187293637545
2212	11	-1.1202674596249693
2212	12	-1.2036826140827728
2212	13	-1.3226456722700206
2212	14	-6.755602944760689
2212	15	-5.385085713046834
2212	16	-3.1352423595238044
2212	17	-7.654035583528842
2212	18	-3.359034453232187
2212	19	-2.17978165643877
2212	20	-2.1293925367889983
2212	21	-1.8606543869347405
2212	22	-1.5665518551035973
2212	23	-1.0987732281215514
2212	24	-1.5089901998767061
2212	25	-1.32041474778611
2213	1	-0.3872421997763027
2213	2	-0.3626611551642114
2213	3	-0.3289752713152758
2213	4	0.07410209155230518
2213	5	-0.1352659410066508
2213	6	-0.5608406413549716
2213	7	-0.5224243256623637
2213	8	-0.5035755220636986
2213	9	-0.4090856604490938
2213	10	-4.247665072696552
2213	11	-1.7434188347833697
2213	12	-1.1819620726682984
2213	13	-0.923333038010071
2213	14	-0.13241691377235298
2213	15	-0.6927616780233521
2213	16	-0.42842854621224546
2213	17	-0.1371971193747754
2213	18	-6.470894509433833E-4
2213	19	-0.5510977793856
2213	20	-0.9049976702717046
2213	21	-0.8905722877758677
2213	22	-1.0704462412107318
2213	23	-1.422191980541804
2213	24	-0.864515820831329
2213	25	-0.5736779206428579
2214	1	-0.9585238774936258
2214	2	-0.714519449101715
2214	3	-0.6891153619009103
2214	4	-0.2569086221316752
2214	5	-3.562144056746149
2214	6	-1.4192314579141083
2214	7	-1.0053446221965174
2214	8	-0.7588932159952506
2214	9	-0.7535115219541595
2214	10	-4.469671980594548
2214	11	-1.837002517475966
2214	12	-1.3189562450585157
2214	13	-0.9971685071425682
2214	14	-0.25719763508772786
2214	15	-0.6009656740986387
2214	16	-0.6525910750777442
2214	17	-0.3404843174639538
2214	18	0.04909076721760641
2214	19	-0.7525063661902252
2214	20	-0.9666494210323648
2214	21	-0.8678277610709486
2214	22	-0.6140851771763991
2214	23	-0.36685719030602826
2214	24	-0.9458402145319624
2214	25	-0.6639825817395959
2215	1	-2.174843960829392
2215	2	-4.737206054045394
2215	3	-3.050491034866436
2215	4	-1.8777191987645594
2215	5	-1.8700826160888084
2215	6	-2.150368222032995
2215	7	-2.239327069113777
2215	8	-2.335181464289107
2215	9	-8.179369218343268
2215	10	-3.9233230315403524
2215	11	-3.1858834232656577
2215	12	-3.5086183749883197
2215	13	-2.883964022977338
2215	14	-7.732087380699454
2215	15	-4.757180078724939
2215	16	-9.390574320304925
2215	17	-4.659947746107701
2215	18	-3.140364240230042
2215	19	-3.621814513819146
2215	20	-3.4059433061730893
2215	21	-3.532366737092442
2215	22	-9.312276567111855
2215	23	-10.59880443370293
2215	24	-6.613753997060659
2215	25	-5.130819132651325
2216	1	-1.9605893004046813
2216	2	-1.9124875572954103
2216	3	-1.8183668265581936
2216	4	-6.951749489097015
2216	5	-3.6338677883855404
2216	6	-2.842965192999749
2216	7	-2.287516087226372
2216	8	-7.964982837186909
2216	9	-4.09491801208577
2216	10	-2.363570018093501
2216	11	-2.3032408863740024
2216	12	-2.5418400597805295
2216	13	-2.3673358366695005
2216	14	-1.7199757802587783
2216	15	-2.208930010043048
2216	16	-2.0007680541114894
2216	17	-1.7449525700276851
2216	18	-7.501818298289134
2216	19	-5.050225552502081
2216	20	-4.331570762649122
2216	21	-3.4795822761273456
2216	22	-3.1457965209354235
2216	23	-2.375147523102239
2216	24	-2.7994177971255922
2216	25	-2.235808536677517
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118290.0
3	2.0
4	988.0
5	5624.0
6	6737.0
7	6268.0
8	5737.0
9	5381.0
10	4967.0
11	4888.0
12	4726.0
13	4644.0
14	4561.0
15	4718.0
16	4787.0
17	5214.0
18	5808.0
19	7198.0
20	9043.0
21	11691.0
22	14495.0
23	17334.0
24	20231.0
25	22883.0
26	25893.0
27	29055.0
28	32773.0
29	38680.0
30	46475.0
31	55157.0
32	71020.0
33	92488.0
34	120535.0
35	161669.0
36	229540.0
37	334289.0
38	918125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70033718317835	18.479501199322936	12.332741332333184	25.487420285165534
2	14.533424660335115	20.158418270852714	41.33363321304068	23.974523855771494
3	18.409437008369878	25.02523067461181	29.552439728539206	27.012892588479108
4	11.442257064594973	16.744949324220503	39.156161711769855	32.65663189941467
5	12.766756122065523	37.91372781215102	36.196151441461375	13.12336462432208
6	31.648296511438172	37.23166791763208	18.33359168488725	12.78644388604249
7	27.93492688840682	31.431947844915147	22.636651331574924	17.99647393510311
8	25.123353854236864	36.33656185396274	20.900605510784487	17.639478781015907
9	26.511465686564456	14.878490033429262	20.12580335271646	38.484240927289825
10	13.90539990935766	28.67377854142522	35.647772511384346	21.773049037832777
11	34.51750600068206	22.372916894428787	24.523983963370956	18.585593141518192
12	23.092886883173996	24.673325669116334	31.763392729655216	20.47039471805445
13	28.92499710347371	21.706282443134253	25.71230069043396	23.65641976295808
14	20.611388948835113	21.37788926642546	28.66300788448115	29.347713900258277
15	23.304933813109912	29.987804295874554	23.398900713748585	23.308361177266946
16	22.44250433017514	27.67534659757967	27.429650461246258	22.45249861099893
17	21.743105939993747	27.981319347597065	28.041012241327394	22.23456247108179
18	21.74205369247446	27.210154432262645	29.367750979455405	21.680040895807487
19	23.54531977059643	26.8539498193965	27.345662945807526	22.25506746419955
20	23.7722219865077	27.02196823075944	27.503777778503053	21.702032004229807
21	23.547807354852136	26.55600593218783	26.82050643911654	23.0756802738435
22	23.387919412699812	27.338208460230256	27.09024941978319	22.18362270728674
23	22.660469913217167	27.120369977512837	27.533477808203084	22.68568230106692
24	23.157776188204725	26.852241386416004	27.477656320069464	22.512326105309803
25	23.01102135522726	26.809478210454724	27.453794805238257	22.725705629079766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	213.0
1	213.0
2	351.5
3	490.0
4	490.0
5	490.0
6	1012.5
7	1535.0
8	1535.0
9	1535.0
10	2559.5
11	3584.0
12	3584.0
13	3584.0
14	6866.0
15	10148.0
16	10148.0
17	10148.0
18	18853.0
19	27558.0
20	27558.0
21	27558.0
22	43704.5
23	59851.0
24	59851.0
25	59851.0
26	85190.5
27	110530.0
28	110530.0
29	110530.0
30	141281.0
31	172032.0
32	172032.0
33	172032.0
34	199764.5
35	227497.0
36	227497.0
37	227497.0
38	250879.0
39	274261.0
40	274261.0
41	274261.0
42	289936.0
43	305611.0
44	305611.0
45	305611.0
46	315412.5
47	325214.0
48	325214.0
49	325214.0
50	314311.5
51	303409.0
52	303409.0
53	303409.0
54	272299.0
55	241189.0
56	241189.0
57	241189.0
58	207130.5
59	173072.0
60	173072.0
61	173072.0
62	141438.5
63	109805.0
64	109805.0
65	109805.0
66	84781.5
67	59758.0
68	59758.0
69	59758.0
70	44008.0
71	28258.0
72	28258.0
73	28258.0
74	19875.5
75	11493.0
76	11493.0
77	11493.0
78	7958.0
79	4423.0
80	4423.0
81	4423.0
82	2917.0
83	1411.0
84	1411.0
85	1411.0
86	919.0
87	427.0
88	427.0
89	427.0
90	270.0
91	113.0
92	113.0
93	113.0
94	68.5
95	24.0
96	24.0
97	24.0
98	16.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1746798623442747
2	0.26465039149007674
3	0.26420176237828896
4	0.46490211320625435
5	0.2600009625133671
6	0.01933183627158212
7	0.018597715906838494
8	0.017537319824431036
9	0.019127913948042224
10	0.02124870611285714
11	0.021452628436397034
12	0.028385987436753492
13	0.028630694225001363
14	0.026224410807230595
15	0.04286447240808609
16	0.02088164593048533
17	0.042252705437466405
18	0.032913063019339175
19	0.028059711719089657
20	0.030098934954488614
21	0.03584954447831368
22	0.04510761796702494
23	0.030098934954488614
24	0.04979783140844255
25	0.0385413191490403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2451914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.26938867949403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.46135133825803	62.72892930936389
2	10.52029121828199	15.626711950241056
3	2.582486854447125	5.753991598578525
4	0.9769300743202173	2.9022399760950086
5	0.45241638941846946	1.6800344335346817
6	0.2637048712648112	1.175111974838532
7	0.17090780192153399	0.8885252578487872
8	0.10796793911191105	0.6414970267861174
9	0.08278650172322484	0.5533652586508009
>10	0.35435109257886804	4.4196354582703
>50	0.015127238517913954	0.7773243683838428
>100	0.009958732206406938	1.4553282711407018
>500	0.0011096438383236568	0.5751573227073743
>1k	6.103041110679306E-4	0.822147793560491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.078446470797916E-5
2	0.0	0.0	0.0	0.0	4.078446470797916E-5
3	0.0	0.0	0.0	0.0	4.078446470797916E-5
4	0.0	0.0	0.0	0.0	4.078446470797916E-5
5	0.0	0.0	0.0	0.0	4.078446470797916E-5
6	0.0	0.0	0.0	0.0	4.078446470797916E-5
7	0.0	0.0	0.0	0.0	4.078446470797916E-5
8	4.078446470797916E-5	0.0	0.0	0.0	4.078446470797916E-5
9	4.078446470797916E-5	0.0	0.0	0.0	4.078446470797916E-5
10	4.078446470797916E-5	0.0	0.0	4.078446470797916E-5	4.078446470797916E-5
11	4.078446470797916E-5	0.0	0.0	4.078446470797916E-5	4.078446470797916E-5
12	4.078446470797916E-5	0.0	0.0	4.078446470797916E-5	1.2235339412393746E-4
13	4.078446470797916E-5	0.0	0.0	4.078446470797916E-5	2.4470678824787493E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAGCGT	65	5.5292727E-5	13.134896	6
ATACCGT	60	4.1436075E-4	12.648418	6
TAGGACC	610	0.0	12.029489	4
CGCAGAT	205	0.0	11.106607	8
GGACGTG	1230	0.0	11.105928	6
TTATACT	315	0.0	10.891263	4
TGGACCG	105	3.4207224E-6	10.869658	5
AGGACGT	1265	0.0	10.826695	5
GGCGAGG	650	0.0	10.512205	19
GTAGGAC	1460	0.0	10.443676	3
GTCCTAC	1340	0.0	10.409082	1
TGGCGAG	1415	0.0	10.395397	18
TAGGACG	1365	0.0	10.262919	4
TTAGGAC	940	0.0	10.239525	3
ATTGGAC	205	3.6379788E-12	10.22716	3
TATGGCG	1480	0.0	10.130381	16
TGTAGGA	1575	0.0	10.0513735	2
ATGGCGA	1545	0.0	10.011278	17
AGGACCT	1200	0.0	9.986499	5
GTGCGCC	125	2.610208E-6	9.868383	11
>>END_MODULE