FastQCFastQC Report
Thu 26 May 2016
SRR1513117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451914
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32340.1318969588656046No Hit
GTCCTACAGTGGACATTTCTAAATT25130.10249135981115162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5750.013.7444944
GTAGGAC14900.010.9922693
AGGACGT13250.010.9231075
ATAGGAC2200.010.8208773
GGACGTG13300.010.8091236
GTCCTAC14750.010.5919231
GACGTGA7850.010.5181487
TTAGGAC9500.010.4244913
TGTAGGA17150.010.2729992
TAGGACG14550.010.2089264
CTGTAGG16700.010.153751
ACTGCGT750.002675517710.1209438
TCCTACA16200.010.1112082
CCGTGTA951.6671866E-49.9865519
CCTTATA3250.09.9628862
TGGCGAG14050.09.79468118
ACGTGAA8700.09.7065098
CCTATTC2500.09.5223733
GTAACAC1901.0077201E-99.5223723
TGGACCG900.00109089499.5217885
Produced by FastQC (version 0.11.5)