##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451914 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.730003580876 31.0 31.0 34.0 27.0 34.0 2 30.840099204131956 33.0 31.0 34.0 27.0 34.0 3 30.9511389877459 33.0 31.0 34.0 27.0 34.0 4 34.343936206571684 37.0 35.0 37.0 31.0 37.0 5 34.0874357746642 37.0 35.0 37.0 30.0 37.0 6 33.999658634030396 37.0 35.0 37.0 30.0 37.0 7 33.871072558009786 37.0 35.0 37.0 29.0 37.0 8 33.838675418469 37.0 35.0 37.0 29.0 37.0 9 35.390230244617065 39.0 35.0 39.0 29.0 39.0 10 35.23819799552513 39.0 35.0 39.0 28.0 39.0 11 35.24814981275852 39.0 35.0 39.0 29.0 39.0 12 35.18191665776206 39.0 35.0 39.0 28.0 39.0 13 35.153451956308416 39.0 35.0 39.0 28.0 39.0 14 36.31898100830617 40.0 37.0 41.0 28.0 41.0 15 36.30112189905519 40.0 37.0 41.0 28.0 41.0 16 36.25958659235194 40.0 36.0 41.0 27.0 41.0 17 36.197190847639845 40.0 36.0 41.0 27.0 41.0 18 36.14987760582141 40.0 36.0 41.0 27.0 41.0 19 36.22315750063012 40.0 37.0 41.0 27.0 41.0 20 36.1874135879154 40.0 37.0 41.0 27.0 41.0 21 36.133478172562334 40.0 37.0 41.0 27.0 41.0 22 36.076690699592234 40.0 37.0 41.0 27.0 41.0 23 35.99539176333265 40.0 37.0 41.0 27.0 41.0 24 35.932056344553686 40.0 37.0 41.0 27.0 41.0 25 35.880178097600485 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5597627063319877 1101 2 0.54586859741271 1101 3 0.4864905970346278 1101 4 0.6243606589611588 1101 5 0.45759388654136757 1101 6 0.5323827924757722 1101 7 0.518231487153578 1101 8 0.4637873270149484 1101 9 0.6136382261806688 1101 10 0.6271930657200144 1101 11 0.6640728507243949 1101 12 0.6589012404858536 1101 13 0.6214464146698049 1101 14 0.8056141201788165 1101 15 0.7608274780161608 1101 16 0.7242101464682733 1101 17 0.7540462095076563 1101 18 0.742204365988762 1101 19 0.7644449790256687 1101 20 0.6680883521243146 1101 21 0.7960732655186433 1101 22 0.7702420257641052 1101 23 0.7916802382811596 1101 24 0.7701260027353882 1101 25 0.7415705474776004 1102 1 0.5252615185019707 1102 2 0.5554076215270349 1102 3 0.4716395114772354 1102 4 0.5821092242398294 1102 5 0.5950372849887771 1102 6 0.5797787388720792 1102 7 0.6219556476466863 1102 8 0.7182217395359274 1102 9 0.7052757194160009 1102 10 0.7394821379896115 1102 11 0.8221563626653747 1102 12 0.8160077354853428 1102 13 0.7568356468454738 1102 14 0.8362749633699664 1102 15 0.8568041433477305 1102 16 0.8725242533637996 1102 17 0.9634536092147528 1102 18 0.9180013739412587 1102 19 0.885381667838665 1102 20 0.9257074332300164 1102 21 0.9088185134342055 1102 22 0.907990866215755 1102 23 0.8565935435060865 1102 24 0.9570033092199921 1102 25 1.053733651673376 1103 1 0.41208107899270985 1103 2 0.3922128179816866 1103 3 0.38621522809143727 1103 4 0.410713160892918 1103 5 0.476742039031123 1103 6 0.33538101534499276 1103 7 0.4208614104979631 1103 8 0.4000262858289503 1103 9 0.5255662055856973 1103 10 0.4146158747255626 1103 11 0.4888535718087681 1103 12 0.549934832710214 1103 13 0.5794085027484854 1103 14 0.6464137048384799 1103 15 0.6133827206731155 1103 16 0.6632670101096068 1103 17 0.5877531734552548 1103 18 0.6328906801431984 1103 19 0.523778701892013 1103 20 0.6212557916474921 1103 21 0.5086946803231385 1103 22 0.5253976587416176 1103 23 0.5819304935525054 1103 24 0.6590073190540622 1103 25 0.6144595042167182 1104 1 0.299978712478179 1104 2 0.39995753954666213 1104 3 0.3624746611795402 1104 4 0.525683703368216 1104 5 0.5217450343463597 1104 6 0.42855255855726426 1104 7 0.489460594220688 1104 8 0.5052221880653107 1104 9 0.5180283547136142 1104 10 0.5605609936546614 1104 11 0.554384173586989 1104 12 0.5275798684600588 1104 13 0.606174553543859 1104 14 0.6305967164447921 1104 15 0.6127168556303886 1104 16 0.6141955578342433 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-0.9462263227525298 2215 21 -1.0081482993669582 2215 22 -0.9688818555256873 2215 23 -1.0154437017771372 2215 24 -1.0179305499802354 2215 25 -1.0326092917687717 2216 1 -0.5440536688911557 2216 2 -0.3317204378067622 2216 3 -0.4967123333484693 2216 4 -0.3800381954989476 2216 5 -0.5573098717603386 2216 6 -0.693009887084898 2216 7 -0.8707159842925236 2216 8 -0.7516586677342261 2216 9 -0.9594925014537381 2216 10 -0.796437000485362 2216 11 -0.8851057382374137 2216 12 -0.783444456008553 2216 13 -0.8383546568400178 2216 14 -1.0382610365884588 2216 15 -0.9296763169361526 2216 16 -0.9923809196324598 2216 17 -1.0387981748010873 2216 18 -0.9650025293242805 2216 19 -1.0058380636624022 2216 20 -0.9921215507255354 2216 21 -1.152032928591126 2216 22 -1.0408581642047707 2216 23 -1.1072232888717934 2216 24 -1.0880445850436118 2216 25 -1.0982923042628627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60542.0 3 0.0 4 964.0 5 8147.0 6 9068.0 7 6863.0 8 5638.0 9 4943.0 10 4384.0 11 4042.0 12 3743.0 13 3617.0 14 3455.0 15 3625.0 16 3699.0 17 3808.0 18 4122.0 19 4892.0 20 6468.0 21 8282.0 22 10612.0 23 12913.0 24 14920.0 25 16592.0 26 18251.0 27 20305.0 28 22747.0 29 27543.0 30 34966.0 31 44408.0 32 57169.0 33 74532.0 34 98649.0 35 132453.0 36 191076.0 37 312406.0 38 1212070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.8044772063736 19.3567974306221 13.28071720294122 27.558008160063093 2 13.847875112728685 20.48588663379401 39.60450841320024 26.061729840277064 3 16.726629781975173 24.960304431261303 30.072987639617665 28.24007814714585 4 11.269788507931718 17.276766869301532 37.67279193593448 33.78065268683227 5 12.76925582471784 38.08257892925847 36.00245229794767 13.145712948076019 6 31.794216844987844 37.65152116569915 17.91115014089187 12.643111848421135 7 27.594955505956815 31.586915450914994 22.679580471331295 18.1385485717969 8 25.16433541188991 36.76842443181656 20.447791817473174 17.61944833882036 9 26.264471540238887 14.739486784172412 19.77624556702334 39.219796108565355 10 14.361051722267405 28.953889876361536 34.898846818656786 21.78621158271427 11 34.52115367831009 22.289484867740057 24.410440170413807 18.778921283536047 12 22.823802139879295 24.811106751704994 31.769058784280364 20.59603232413535 13 28.792567765427336 21.644560127312783 25.799681391761702 23.76319071549818 14 21.121698033176283 21.448427976664362 27.54874642721969 29.881127562939664 15 23.226426375476464 30.0651246332457 23.28164854069107 23.426800450586764 16 22.631421819851756 27.904363693016965 26.822800473426067 22.641414013705212 17 22.05456971493816 28.30642322266201 27.283592902600233 22.355414159799594 18 22.031677338920762 27.46555179143739 28.648393287250084 21.85437758239176 19 23.465586476524052 26.978882619863505 27.260295426348556 22.295235477263883 20 23.668815464163913 27.006208211217846 27.48346801723062 21.841508307387617 21 23.32032156509915 26.54665822145673 26.90405700435135 23.22896320909277 22 23.335305947574682 27.44406700580405 26.803912220161696 22.416714826459568 23 22.69997503829908 27.153721659088426 27.136631867118915 23.00967143549358 24 23.031272711848786 26.911098839518843 27.523966990685643 22.533661457946728 25 22.975269035599243 26.895117512661574 27.221231156496213 22.908382295242966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 159.0 1 159.0 2 276.0 3 393.0 4 393.0 5 393.0 6 942.0 7 1491.0 8 1491.0 9 1491.0 10 2389.0 11 3287.0 12 3287.0 13 3287.0 14 6362.5 15 9438.0 16 9438.0 17 9438.0 18 18076.5 19 26715.0 20 26715.0 21 26715.0 22 42675.5 23 58636.0 24 58636.0 25 58636.0 26 83590.5 27 108545.0 28 108545.0 29 108545.0 30 139139.0 31 169733.0 32 169733.0 33 169733.0 34 197132.0 35 224531.0 36 224531.0 37 224531.0 38 248125.0 39 271719.0 40 271719.0 41 271719.0 42 287145.5 43 302572.0 44 302572.0 45 302572.0 46 313920.0 47 325268.0 48 325268.0 49 325268.0 50 316166.5 51 307065.0 52 307065.0 53 307065.0 54 275964.5 55 244864.0 56 244864.0 57 244864.0 58 210696.5 59 176529.0 60 176529.0 61 176529.0 62 144389.0 63 112249.0 64 112249.0 65 112249.0 66 86948.0 67 61647.0 68 61647.0 69 61647.0 70 45374.5 71 29102.0 72 29102.0 73 29102.0 74 20419.0 75 11736.0 76 11736.0 77 11736.0 78 7999.0 79 4262.0 80 4262.0 81 4262.0 82 2855.5 83 1449.0 84 1449.0 85 1449.0 86 925.0 87 401.0 88 401.0 89 401.0 90 253.0 91 105.0 92 105.0 93 105.0 94 61.0 95 17.0 96 17.0 97 17.0 98 9.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1887912871332355 2 0.009421211347543186 3 0.005261195947329311 4 0.001998438770690979 5 0.080223042080595 6 0.33426947274659713 7 0.02801892725438168 8 0.015212605336076225 9 0.004853351300249519 10 0.0035890328943021656 11 0.0 12 0.0 13 0.0 14 0.0017129475177351243 15 0.0 16 0.0 17 0.019617327524537976 18 0.02846755636616945 19 0.0 20 0.0 21 0.0012643184059473538 22 2.854912529558541E-4 23 0.0064439454238607065 24 0.0 25 3.2627571766383326E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2451914.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.54623037851862 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75995302711956 59.21162665393677 2 11.579675690566091 16.569642893315464 3 2.838497847410059 6.092514627591878 4 1.1282120186108617 3.22877267997385 5 0.528904523753777 1.89205624523642 6 0.3085984951970662 1.3247435419100082 7 0.17788120485291437 0.8908710763690554 8 0.13891114312989952 0.7950854918812132 9 0.09207958562489064 0.5929152521649262 >10 0.41402363092082956 5.0170849362844026 >50 0.018982119461498307 0.9143379552549492 >100 0.012001029479916672 1.634341342437023 >500 0.0014248024204052785 0.6775697551462697 >1k 8.548814522254081E-4 1.158437548497956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3234 0.1318969588656046 No Hit GTCCTACAGTGGACATTTCTAAATT 2513 0.10249135981115162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.078446470797916E-5 0.0 12 0.0 0.0 0.0 4.078446470797916E-5 1.2235339412393746E-4 13 0.0 0.0 0.0 4.078446470797916E-5 1.6313785883191663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 575 0.0 13.744494 4 GTAGGAC 1490 0.0 10.992269 3 AGGACGT 1325 0.0 10.923107 5 ATAGGAC 220 0.0 10.820877 3 GGACGTG 1330 0.0 10.809123 6 GTCCTAC 1475 0.0 10.591923 1 GACGTGA 785 0.0 10.518148 7 TTAGGAC 950 0.0 10.424491 3 TGTAGGA 1715 0.0 10.272999 2 TAGGACG 1455 0.0 10.208926 4 CTGTAGG 1670 0.0 10.15375 1 ACTGCGT 75 0.0026755177 10.120943 8 TCCTACA 1620 0.0 10.111208 2 CCGTGTA 95 1.6671866E-4 9.986551 9 CCTTATA 325 0.0 9.962886 2 TGGCGAG 1405 0.0 9.794681 18 ACGTGAA 870 0.0 9.706509 8 CCTATTC 250 0.0 9.522373 3 GTAACAC 190 1.0077201E-9 9.522372 3 TGGACCG 90 0.0010908949 9.521788 5 >>END_MODULE