FastQCFastQC Report
Thu 26 May 2016
SRR1513116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426708
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT29220.12041003697189773No Hit
GTACATGGGAAGCAGTGGTATCAAC27520.11340466178872777No Hit
GTATCAACGCAGAGTACTTTTTTTT24640.10153673206665162No Hit
GTCCTAAAGTGTGTATTTCTCATTT24430.10067136219108355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT604.1415612E-412.6491496
GAACCGT550.003101274612.0741876
TAGGACC6850.011.8249034
GTCCTAC16250.011.2280011
ACACCGT951.3819894E-510.9847866
GTAGGAC17550.010.8057313
TGTAGGA19050.010.5621782
TCCTACA18550.010.4870242
GACGTGG7600.010.4863437
AGGACGT13950.010.3638525
GGACGTG13300.010.2001586
GGCGAGG5700.010.155745519
CCTACAG17950.010.0871233
CTGTAGG19200.09.95064451
GCCTTAC1553.732748E-89.8607671
TAGGACG15150.09.8125344
GACGTGA6100.09.7987137
GTCCTAA8950.09.7127181
GCCCTAA1606.096525E-89.5526181
GTATAGG4000.09.5526171
Produced by FastQC (version 0.11.5)