##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426708 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.732575571514992 31.0 30.0 34.0 25.0 34.0 2 29.592002004361465 31.0 30.0 34.0 24.0 34.0 3 29.7919016214559 31.0 30.0 34.0 25.0 34.0 4 32.88473314465523 37.0 35.0 37.0 25.0 37.0 5 33.049455064226926 37.0 35.0 37.0 26.0 37.0 6 33.406439505700725 37.0 35.0 37.0 28.0 37.0 7 33.38099557095456 37.0 35.0 37.0 28.0 37.0 8 33.264474341371105 37.0 35.0 37.0 27.0 37.0 9 34.543088414428105 39.0 35.0 39.0 27.0 39.0 10 34.132751447640175 39.0 34.0 39.0 24.0 39.0 11 34.57680281269934 39.0 35.0 39.0 27.0 39.0 12 34.56984441473799 39.0 35.0 39.0 27.0 39.0 13 34.60754322316488 39.0 35.0 39.0 27.0 39.0 14 34.86818356390633 40.0 34.0 41.0 18.0 41.0 15 35.2989951819502 40.0 35.0 41.0 25.0 41.0 16 35.141583989503474 40.0 35.0 41.0 24.0 41.0 17 34.76495853642054 39.0 34.0 41.0 18.0 41.0 18 34.50093913235544 39.0 34.0 41.0 17.0 41.0 19 35.14034445017695 40.0 34.0 41.0 25.0 41.0 20 35.355103292196674 40.0 36.0 41.0 26.0 41.0 21 35.40468733774315 40.0 36.0 41.0 26.0 41.0 22 35.0967788460746 40.0 36.0 41.0 23.0 41.0 23 34.63601677663732 40.0 34.0 41.0 18.0 41.0 24 34.89068441691378 40.0 35.0 41.0 22.0 41.0 25 34.53443595191511 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.731618674727855 1101 2 0.9741733757731801 1101 3 0.8952838014340294 1101 4 1.2737102429008331 1101 5 1.0932730783703803 1101 6 0.8592322514982769 1101 7 0.9348800688401226 1101 8 0.8223658752091012 1101 9 1.1203820814018144 1101 10 1.5615073414124012 1101 11 1.1730751684817022 1101 12 1.0356870449221987 1101 13 0.8931509987730877 1101 14 1.8904572514137925 1101 15 1.488776148284721 1101 16 1.5730375353298953 1101 17 1.8389749046483743 1101 18 1.9195116646336388 1101 19 1.5722408682836644 1101 20 1.1547878673200387 1101 21 1.1187120372898178 1101 22 1.404356646208015 1101 23 1.6928504534518254 1101 24 1.3781428343624214 1101 25 1.5006551308428584 1102 1 0.8046066785366293 1102 2 1.0698456659092948 1102 3 0.9777128829340178 1102 4 1.3332551166528717 1102 5 1.2632679685963097 1102 6 0.903572376421991 1102 7 0.9146188482503916 1102 8 0.9947776402525079 1102 9 1.2989159114583373 1102 10 1.42437355321254 1102 11 1.0836893047381153 1102 12 0.967801567280631 1102 13 0.9115584162805845 1102 14 1.9402092220138627 1102 15 1.6021702492740317 1102 16 1.6559483761142246 1102 17 1.9896030112445402 1102 18 2.1967773073523134 1102 19 1.5881125755068979 1102 20 1.301935770817785 1102 21 1.2374269321302407 1102 22 1.4619349213331034 1102 23 1.7640221016952253 1102 24 1.5783898822750047 1102 25 1.8369610118243855 1103 1 0.7252747847957437 1103 2 0.995223077251346 1103 3 0.7701068550235135 1103 4 1.2786849046563376 1103 5 1.119242672476986 1103 6 0.8030340113326488 1103 7 0.8393946097424276 1103 8 0.8126124566525945 1103 9 1.222311338949119 1103 10 1.4909039180410275 1103 11 1.0832881060328603 1103 12 1.0319585312720463 1103 13 0.9931509987730891 1103 14 1.8318651189086168 1103 15 1.5720938332960372 1103 16 1.5634788180108146 1103 17 1.9095283737609918 1103 18 2.1535425520138674 1103 19 1.5306732073466875 1103 20 1.226557692540318 1103 21 1.3180463817454964 1103 22 1.5246802053586705 1103 23 1.8473962909981694 1103 24 1.631630002738362 1103 25 1.7644638722362842 1104 1 0.6274337430682841 1104 2 0.876444626711514 1104 3 0.7464184978598141 1104 4 1.1081344961357615 1104 5 1.0643674171516295 1104 6 0.7844415835532459 1104 7 0.6646681726254187 1104 8 0.6527572626053271 1104 9 0.9808884472070716 1104 10 1.1532375918081712 1104 11 0.756758342391187 1104 12 0.7337625872977256 1104 13 0.678251669914026 1104 14 1.6734262510941988 1104 15 1.3089846999432524 1104 16 1.3738275194989953 1104 17 1.8521093483743556 1104 18 1.924665254085646 1104 19 1.2818480687414961 1104 20 1.0309456411834432 1104 21 0.9608841407183562 1104 22 1.2770583786881105 1104 23 1.5446599326672725 1104 24 1.3222529966105512 1104 25 1.561425865233069 1105 1 0.7254704340374829 1105 2 0.9744866766696028 1105 3 0.8968553097683554 1105 4 1.2397369243370804 1105 5 1.1431309390936093 1105 6 0.8784173196447753 1105 7 0.7223421179818388 1105 8 0.9013428697371282 1105 9 0.9958262666417355 1105 10 1.4731849433632789 1105 11 0.9846654942877464 1105 12 0.7975188402057896 1105 13 0.8161345434183502 1105 14 1.6731624295195644 1105 15 1.2104944502252337 1105 16 1.4316011130812427 1105 17 1.7938198434833765 1105 18 1.5026818126371282 1105 19 1.2417807917053025 1105 20 1.1490305992620335 1105 21 1.0913246097747233 1105 22 1.3144199456854295 1105 23 1.6070359562613064 1105 24 1.3737239847465474 1105 25 1.636330625499788 1106 1 0.5415053134372947 1106 2 0.6850056422238886 1106 3 0.5991541575990951 1106 4 1.1749173571147367 1106 5 1.051645723767109 1106 6 0.7930904287192888 1106 7 0.704309582867289 1106 8 0.7834442596770685 1106 9 0.8891525336976827 1106 10 0.8836958718227876 1106 11 0.7464873843100506 1106 12 0.7012638876445436 1106 13 0.6041995372552549 1106 14 1.7133224393232283 1106 15 1.1954364359287482 1106 16 1.3003394403947155 1106 17 1.6991388882945557 1106 18 1.79065808013074 1106 19 1.2540496953898739 1106 20 0.9683288574405751 1106 21 0.8623445966766425 1106 22 1.12596041802788 1106 23 1.4935155734727843 1106 24 1.1774835944461302 1106 25 1.5211009675060225 1107 1 0.59145521957835 1107 2 0.8399846714542427 1107 3 0.7671039564727877 1107 4 1.0236566437867722 1107 5 0.9188354261001734 1107 6 0.670394156260187 1107 7 0.643306203945329 1107 8 0.6509102827395523 1107 9 1.024616411412893 1107 10 1.1215024687656694 1107 11 0.7543243379169198 1107 12 0.6805831689532056 1107 13 0.7552843321064202 1107 14 1.544381060937603 1107 15 0.9689489057598024 1107 16 1.0842991078658812 1107 17 1.5157979389783875 1107 18 1.6484773346225623 1107 19 1.101214511694515 1107 20 0.9143156635548095 1107 21 0.8219275411657847 1107 22 1.0031845531847594 1107 23 1.290664406940202 1107 24 1.0317778288253194 1107 25 1.3504066258594705 1108 1 0.5668429028628097 1108 2 0.741762188560795 1108 3 0.5630096171854859 1108 4 1.0335454201130787 1108 5 0.9265762241095956 1108 6 0.587730048911034 1108 7 0.5603888663464645 1108 8 0.5429616823663181 1108 9 0.7770652161760623 1108 10 1.0969094180236425 1108 11 0.7049181351265474 1108 12 0.3515392517755629 1108 13 0.45366819418764237 1108 14 1.5653280795104223 1108 15 1.0139328211601395 1108 16 1.0477169342233026 1108 17 1.3451046838464222 1108 18 1.6678982890347172 1108 19 1.0086433573356786 1108 20 0.8297361203218969 1108 21 0.905578691970014 1108 22 1.0082266219664149 1108 23 1.329074075473038 1108 24 1.0011404521257745 1108 25 1.3874949045407092 1109 1 0.25148078549046815 1109 2 0.4087828960436788 1109 3 0.3176261047195723 1109 4 0.5726483881276465 1109 5 0.6443157634281818 1109 6 0.30891204352161594 1109 7 0.24647140589827643 1109 8 0.28883944385805904 1109 9 0.4563547158957988 1109 10 0.8396801873630935 1109 11 0.34723689894665455 1109 12 0.34151028834956776 1109 13 0.2512069242058459 1109 14 1.182693000573643 1109 15 0.6902999931987779 1109 16 0.9436603151450669 1109 17 1.2861436114151346 1109 18 1.3846264691099108 1109 19 0.8090248978463137 1109 20 0.6055834842383447 1109 21 0.4245579894569502 1109 22 0.7998727023192416 1109 23 1.1799782107573336 1109 24 0.8696995332194604 1109 25 1.108486696875893 1110 1 0.48058986010122595 1110 2 0.6479742139379034 1110 3 0.5791719303289966 1110 4 0.9491363823488612 1110 5 0.7671177790413495 1110 6 0.36151049612946196 1110 7 0.35632058303029623 1110 8 0.4800240082297478 1110 9 0.5549850388638689 1110 10 0.9221194622297233 1110 11 0.551657671250247 1110 12 0.43815702516236144 1110 13 0.24282420138746375 1110 14 1.1294581851206047 1110 15 0.8272757031454248 1110 16 0.9764611993691545 1110 17 1.2704959781940701 1110 18 0.9929479228578586 1110 19 0.6838079757468023 1110 20 0.4752725262999107 1110 21 0.2601393058716681 1110 22 0.6987296512239709 1110 23 -0.5784597760663317 1110 24 0.16980135823708764 1110 25 0.6711543382777734 1111 1 0.15251175966274033 1111 2 0.47153361660192417 1111 3 0.4360305387512682 1111 4 0.7440937745884568 1111 5 0.6341573670284433 1111 6 0.3312409917032255 1111 7 0.35375894656136353 1111 8 0.44087905911086267 1111 9 0.6193657785014963 1111 10 0.8940661818458366 1111 11 0.4909931142882229 1111 12 0.35958190313042593 1111 13 0.28450120974355286 1111 14 1.1024072212751506 1111 15 0.727349749641661 1111 16 0.9157261120852951 1111 17 1.2873283187252227 1111 18 1.4305490645803687 1111 19 0.9150740053653976 1111 20 0.5143076466771745 1111 21 0.27619167618688323 1111 22 0.6831259033957267 1111 23 0.9752994280372462 1111 24 0.7341318372641368 1111 25 0.8496053600366835 1112 1 0.1786487818101108 1112 2 0.28459726086769166 1112 3 0.1502244142696405 1112 4 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quality scores pass #Quality Count 2 94911.0 3 1.0 4 849.0 5 4741.0 6 5732.0 7 5225.0 8 4930.0 9 4659.0 10 4252.0 11 4167.0 12 4132.0 13 4194.0 14 4260.0 15 4278.0 16 4244.0 17 4701.0 18 5300.0 19 6453.0 20 8465.0 21 10601.0 22 13514.0 23 16405.0 24 18798.0 25 21606.0 26 24372.0 27 27689.0 28 31519.0 29 37651.0 30 45062.0 31 54112.0 32 69244.0 33 90656.0 34 119859.0 35 161443.0 36 231183.0 37 338327.0 38 939173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.502076888209665 18.5719463633609 12.374142060639686 25.55183468778975 2 14.353686075267442 20.088310799812604 41.70784890326216 23.850154221657792 3 18.492172743332414 24.715267323165804 29.703666490946556 27.08889344255522 4 11.281127094489943 16.71034800755966 39.405798553134915 32.60272634481548 5 12.55659678959875 38.01796390364169 36.48096860654573 12.944470700213827 6 31.576400646928 37.42603769789913 18.327194881289373 12.670366773883496 7 27.95614676819404 31.517532019247614 22.562075609730968 17.96424560282738 8 24.892501326088308 36.786575823606974 20.940819986242477 17.380102864062245 9 26.388088614116434 15.022936630602782 20.266172076249354 38.322802679031426 10 13.642611698012578 28.940028612562735 35.948705118730594 21.468654570694092 11 34.301733450773455 22.47802222486925 24.68196163467083 18.53828268968647 12 22.914891643738216 24.781071918055257 32.02584474603518 20.278191692171347 13 28.68352216003568 21.829166450264093 25.84584552401162 23.64146586568861 14 20.265473302221444 21.681954690731807 28.894365483112278 29.158206523934478 15 23.070783691305603 30.397617182668917 23.506204394607742 23.025394731417734 16 22.014777884529384 28.10914053416196 27.89942927046612 21.976652310842542 17 21.49946427680667 28.305230711388457 28.338994258547967 21.856310753256906 18 21.344709835708777 27.545326834673293 29.814554052538895 21.295409277079035 19 23.19443213598976 27.1534769266281 27.7120769641374 21.94001397324474 20 23.419409462191137 27.338012656265974 27.894238727910064 21.34833915363282 21 23.060037611620853 26.906530752570447 27.18024969433344 22.853181941475263 22 22.996008466240166 27.75884572858065 27.338584469050154 21.90656133612903 23 22.07705485380946 27.595411729208262 27.87265685412933 22.454876562852945 24 22.75942307184886 27.161913902741496 28.030134370300008 22.048528655109635 25 22.631982936805333 27.09903430035213 27.86665545389862 22.402327308943914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 200.0 2 341.5 3 483.0 4 483.0 5 483.0 6 988.5 7 1494.0 8 1494.0 9 1494.0 10 2624.0 11 3754.0 12 3754.0 13 3754.0 14 7298.5 15 10843.0 16 10843.0 17 10843.0 18 19790.5 19 28738.0 20 28738.0 21 28738.0 22 45713.0 23 62688.0 24 62688.0 25 62688.0 26 87928.0 27 113168.0 28 113168.0 29 113168.0 30 146032.0 31 178896.0 32 178896.0 33 178896.0 34 206700.5 35 234505.0 36 234505.0 37 234505.0 38 256350.5 39 278196.0 40 278196.0 41 278196.0 42 292419.0 43 306642.0 44 306642.0 45 306642.0 46 315556.5 47 324471.0 48 324471.0 49 324471.0 50 310489.0 51 296507.0 52 296507.0 53 296507.0 54 263834.0 55 231161.0 56 231161.0 57 231161.0 58 195978.0 59 160795.0 60 160795.0 61 160795.0 62 130430.0 63 100065.0 64 100065.0 65 100065.0 66 77103.5 67 54142.0 68 54142.0 69 54142.0 70 39449.0 71 24756.0 72 24756.0 73 24756.0 74 17283.0 75 9810.0 76 9810.0 77 9810.0 78 6707.0 79 3604.0 80 3604.0 81 3604.0 82 2423.0 83 1242.0 84 1242.0 85 1242.0 86 817.0 87 392.0 88 392.0 89 392.0 90 256.0 91 120.0 92 120.0 93 120.0 94 70.0 95 20.0 96 20.0 97 20.0 98 18.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17859585908152115 2 0.25408907870250563 3 0.25516048902463745 4 0.4419979659687115 5 0.2503803506643568 6 0.01945021815562482 7 0.017843102672427006 8 0.016442027635793017 9 0.018873304905246118 10 0.02064525274569499 11 0.020892501281571576 12 0.028145125000618124 13 0.027774252196803243 14 0.02579626390979055 15 0.04153775402726657 16 0.021222165996073693 17 0.041826210652455925 18 0.031812644949454155 19 0.026496801428107543 20 0.029504991947939346 21 0.03552137298760296 22 0.04425748792190902 23 0.02868083016168406 24 0.048295880674559935 25 0.037169696560113534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2426708.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.81248639765093 #Duplication Level Percentage of deduplicated Percentage of total 1 83.47523732293466 61.615148194397975 2 11.476961101525237 16.94286070385401 3 2.7556650633443214 6.102074700137539 4 0.9772406651898327 2.885302532262235 5 0.43690942771873803 1.6124685595247399 6 0.23381046499519476 1.0354879060251752 7 0.13521401813757797 0.6986338012186191 8 0.09884872724232519 0.5837016267999388 9 0.06622354636200883 0.4399312153544985 >10 0.3142170427972159 3.9267329416913723 >50 0.017744606277114452 0.8961735325457711 >100 0.009613711870906701 1.4110815333386584 >500 0.0015240522764011022 0.7440025659260772 >1k 7.902493284902078E-4 1.1064001869234692 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2922 0.12041003697189773 No Hit GTACATGGGAAGCAGTGGTATCAAC 2752 0.11340466178872777 No Hit GTATCAACGCAGAGTACTTTTTTTT 2464 0.10153673206665162 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2443 0.10067136219108355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 8.241617862552891E-5 0.0 11 0.0 0.0 0.0 8.241617862552891E-5 0.0 12 0.0 0.0 0.0 1.2362426793829335E-4 4.1208089312764455E-5 13 0.0 0.0 0.0 1.2362426793829335E-4 4.1208089312764455E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 60 4.1415612E-4 12.649149 6 GAACCGT 55 0.0031012746 12.074187 6 TAGGACC 685 0.0 11.824903 4 GTCCTAC 1625 0.0 11.228001 1 ACACCGT 95 1.3819894E-5 10.984786 6 GTAGGAC 1755 0.0 10.805731 3 TGTAGGA 1905 0.0 10.562178 2 TCCTACA 1855 0.0 10.487024 2 GACGTGG 760 0.0 10.486343 7 AGGACGT 1395 0.0 10.363852 5 GGACGTG 1330 0.0 10.200158 6 GGCGAGG 570 0.0 10.1557455 19 CCTACAG 1795 0.0 10.087123 3 CTGTAGG 1920 0.0 9.9506445 1 GCCTTAC 155 3.732748E-8 9.860767 1 TAGGACG 1515 0.0 9.812534 4 GACGTGA 610 0.0 9.798713 7 GTCCTAA 895 0.0 9.712718 1 GCCCTAA 160 6.096525E-8 9.552618 1 GTATAGG 400 0.0 9.552617 1 >>END_MODULE