FastQCFastQC Report
Thu 26 May 2016
SRR1513116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426708
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34650.14278602946872881No Hit
GTACATGGGAAGCAGTGGTATCAAC31360.12922856808482933No Hit
GTCCTACAGTGGACATTTCTAAATT30040.12378910029554441No Hit
CCCATGTACTCTGCGTTGATACCAC28920.11917379429251479No Hit
CTGTAGGACGTGGAATATGGCAAGA25390.10462733876510895No Hit
CTTTAGGACGTGAAATATGGCGAGG24880.10252572621015794No Hit
GTCCTAAAGTGTGTATTTCTCATTT24650.10157794015596437No Hit
TATCAACGCAGAGTACTTTTTTTTT24380.10046532174451973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG604.0197637E-412.6939865
TAGGACC5850.012.2057574
GTTTGCG550.003098670912.07554159
CTAATAC1900.011.5255183
AGGACGT14900.011.1817835
GGACGTG14400.011.1063196
TTTGCGG600.00593316511.06924610
CTAGGAC3100.011.0567383
GTAGGAC16900.011.04223
GACGTGG7950.010.8639757
GTCGGTC700.001503694610.84691912
TGTAGGA18950.010.8031742
TAGGACA5700.010.6896724
GTCCTAA9650.010.5627321
TAGGACG16100.010.5257594
GGCAAGA13750.010.21266919
CTGTAGG19000.010.1779941
GACGTGA7450.09.9369437
TTAGGAC9450.09.8737133
ATGGCAA14050.09.7952417
Produced by FastQC (version 0.11.5)