FastQCFastQC Report
Thu 26 May 2016
SRR1513115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175234
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC111060.5105657598217019No Hit
CCCATGTACTCTGCGTTGATACCAC91580.42101217616127734No Hit
GAGTACATGGGAAGCAGTGGTATCA44780.2058629094616947No Hit
GTATCAACGCAGAGTACATGGGAAG30740.1413181294518199No Hit
GCGTTGATACCACTGCTTCCCATGT30490.14016882781346743No Hit
CATGTACTCTGCGTTGATACCACTG28970.1331810738522844No Hit
GCTTCCCATGTACTCTGCGTTGATA25290.11626335373573601No Hit
GTATCAACGCAGAGTACTTTTTTTT24940.11465433144204255No Hit
TATCAACGCAGAGTACATGGGAAGC23080.10610352725270017No Hit
ACGCAGAGTACATGGGAAGCAGTGG22480.10334520332065424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCC400.005284580414.2481378
GCGTTTA1900.013.99784813
TAGGACC7350.012.6685074
CGTTTAT2200.012.52080214
TGTAGGA15200.012.4398792
GTAGGAC14000.012.419093
GTCCTAC14650.012.2063771
GACCCGT550.003071600612.0896066
AAGTCGT550.003072129812.0893297
GGACCGA658.032941E-411.6910486
GTGTAGG4400.011.6736761
CTGTAGG15200.011.5769641
AGGACGT10200.011.363095
TCCTACA16350.011.3324432
TAGGACA5800.011.3033664
GGCGAGG4200.011.08290519
TGGCGAG11150.011.07580418
TATGGCG11850.011.06235416
GGACGTG9900.011.0341646
TAGGACG10700.011.0109454
Produced by FastQC (version 0.11.5)