##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513115_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175234
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.462847675238617	31.0	30.0	34.0	25.0	34.0
2	29.138110198718852	31.0	30.0	34.0	18.0	34.0
3	29.518934054910876	31.0	30.0	34.0	24.0	34.0
4	32.40338740567682	35.0	33.0	37.0	19.0	37.0
5	32.62885694136815	35.0	33.0	37.0	24.0	37.0
6	33.08496832984405	37.0	35.0	37.0	27.0	37.0
7	33.06408735795781	37.0	35.0	37.0	27.0	37.0
8	33.05507545395116	37.0	35.0	37.0	27.0	37.0
9	34.449019737646616	39.0	35.0	39.0	27.0	39.0
10	33.95731539687225	39.0	34.0	39.0	23.0	39.0
11	34.34259348649387	39.0	35.0	39.0	27.0	39.0
12	34.19121896770646	39.0	35.0	39.0	25.0	39.0
13	34.27730303958103	39.0	35.0	39.0	26.0	39.0
14	34.607575552791104	40.0	34.0	41.0	17.0	41.0
15	35.15891761530024	40.0	35.0	41.0	25.0	41.0
16	34.74740602620224	40.0	34.0	41.0	18.0	41.0
17	34.29390493160736	39.0	33.0	41.0	17.0	41.0
18	33.717158245963425	39.0	32.0	41.0	11.0	41.0
19	34.60339301426881	39.0	34.0	41.0	18.0	41.0
20	34.86596154712551	40.0	34.0	41.0	22.0	41.0
21	34.8866793181791	40.0	35.0	41.0	19.0	41.0
22	34.47384557247634	40.0	34.0	41.0	16.0	41.0
23	32.81916152469114	39.0	32.0	41.0	8.0	41.0
24	33.92014882077055	39.0	33.0	41.0	2.0	41.0
25	33.65316513073996	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7964438657291062
1101	2	1.1354339505517466
1101	3	0.9606430681663234
1101	4	0.9060879232676768
1101	5	1.1164972908980815
1101	6	0.7596098694479139
1101	7	0.8162940188265324
1101	8	0.762887417313884
1101	9	0.8935420735791197
1101	10	1.2764492155781113
1101	11	1.0293753452279475
1101	12	1.0464114200178898
1101	13	1.0261821185180509
1101	14	0.03519473795885375
1101	15	0.6807447146426497
1101	16	1.3906480642728027
1101	17	2.006294670221699
1101	18	-4.108536871282322
1101	19	-0.47485790947465745
1101	20	0.448665791416893
1101	21	0.8675473787151802
1101	22	1.3936396402534754
1101	23	3.231729867699407
1101	24	2.0998033509651393
1101	25	2.3028825100037764
1102	1	1.0773118719020758
1102	2	1.2862589931793202
1102	3	1.1053785767402857
1102	4	1.6596457896652694
1102	5	1.447895259995363
1102	6	1.0942266704847157
1102	7	1.063110361828052
1102	8	0.9968490363486637
1102	9	1.1946574811685124
1102	10	1.5894688812472566
1102	11	1.1549767067858738
1102	12	1.2116003679214842
1102	13	1.1181368391807567
1102	14	2.0678328139879554
1102	15	1.7174961241461588
1102	16	1.8473806593593025
1102	17	1.3398919548276282
1102	18	1.7245799366224048
1102	19	1.540692755619439
1102	20	1.6500408439008751
1102	21	1.6013055585131468
1102	22	2.0709831544583963
1102	23	3.4691068899800825
1102	24	2.370015391820786
1102	25	2.7154808559833796
1103	1	0.7044794989739032
1103	2	0.936109670393865
1103	3	0.6206339019382341
1103	4	1.0945682082200818
1103	5	1.033836048018813
1103	6	0.6419505558094016
1103	7	0.641238858975953
1103	8	0.5717844481507299
1103	9	0.6609709802169377
1103	10	0.9654330080881763
1103	11	0.6603497817607789
1103	12	0.7745307856730719
1103	13	0.5934276445657858
1103	14	1.4379994767568363
1103	15	0.9231154359022895
1103	16	1.1619125741989649
1103	17	1.6011414123074275
1103	18	1.5533761997066406
1103	19	1.1682245204647685
1103	20	1.0135358113891115
1103	21	1.0126285244862245
1103	22	1.1820757002097224
1103	23	2.6663807124752736
1103	24	1.697353084475921
1103	25	1.8294238575737722
1104	1	0.6932487598235291
1104	2	1.2014088028052576
1104	3	0.8941134915945987
1104	4	1.430617153514234
1104	5	1.2265182241675205
1104	6	0.8222726669864429
1104	7	0.7415979777331785
1104	8	0.8188106942089064
1104	9	0.8654285060162366
1104	10	1.2844595409004498
1104	11	0.8907553118914038
1104	12	1.0486328799505245
1104	13	0.8238680001297567
1104	14	1.7760130025476215
1104	15	1.2049552598411992
1104	16	1.5286110499471732
1104	17	2.016137114237651
1104	18	2.5299819369930177
1104	19	1.796574071860391
1104	20	1.196279772117137
1104	21	1.2855245102715145
1104	22	1.604221082130337
1104	23	3.1511849217587127
1104	24	2.05082048893545
1104	25	2.1286745457697194
1105	1	0.6687794789517199
1105	2	1.0887145949016492
1105	3	0.7600592442532168
1105	4	0.9324487746589014
1105	5	0.9424876858435169
1105	6	0.6535954690835766
1105	7	0.5467859169976492
1105	8	0.48127285441135115
1105	9	0.7070680192435788
1105	10	1.232844844513231
1105	11	0.7893991954705086
1105	12	0.6979088175663222
1105	13	0.7049593361774029
1105	14	1.4612052156096738
1105	15	0.7872810307779972
1105	16	1.1933236652473127
1105	17	1.6840056820760907
1105	18	1.7330002081040874
1105	19	1.1917780226533807
1105	20	0.9692832236382145
1105	21	0.8002488436159183
1105	22	1.3663484525734688
1105	23	2.7687982474166333
1105	24	1.7726293755530094
1105	25	1.8146831293378085
1106	1	-0.4048836381896592
1106	2	0.07368027551950362
1106	3	-0.1976011916680882
1106	4	-2.354474642699298
1106	5	-0.3285268460405746
1106	6	-0.32991338399049397
1106	7	-0.34768976008672325
1106	8	-0.3202722514815832
1106	9	-0.2730634826270517
1106	10	-0.28551106514702695
1106	11	-0.32388704825051917
1106	12	-0.26044892340011216
1106	13	-0.3304787862172418
1106	14	0.3359016120451983
1106	15	-0.2862447383999509
1106	16	-1.1160543832611722
1106	17	-6.346114608913766
1106	18	-7.294349645332719
1106	19	-2.9201429588328196
1106	20	-1.5589464785045308
1106	21	-1.1347926131646702
1106	22	-1.5897365904680996
1106	23	-5.245878028129209
1106	24	-2.2160787435898897
1106	25	-0.7622100127727265
1107	1	0.393388221890401
1107	2	0.8101150087664202
1107	3	0.581773776762045
1107	4	0.8430087381720242
1107	5	0.8104392971759893
1107	6	0.4225026229194455
1107	7	0.32911373394868804
1107	8	0.3464643591140515
1107	9	0.27003540909488066
1107	10	0.7139109243573145
1107	11	0.45994481102631113
1107	12	0.45153527587709874
1107	13	0.4572139071692476
1107	14	1.1460848760494144
1107	15	0.6748388959326022
1107	16	-5.927950447880161
1107	17	-1.0916834826151103
1107	18	0.616518022375466
1107	19	0.5702072116731998
1107	20	0.605326598989123
1107	21	0.424326917795824
1107	22	0.6135059298735115
1107	23	2.3384696174953348
1107	24	1.4724878921121416
1107	25	1.1377142018597155
1108	1	0.19650389387398448
1108	2	0.5629045955016743
1108	3	0.22110828900861534
1108	4	0.6888817647534893
1108	5	0.49920681015440493
1108	6	0.16984331747108428
1108	7	0.07196061406133225
1108	8	0.026114005340232893
1108	9	0.3034669504679357
1108	10	0.6760268973640393
1108	11	0.260044868443849
1108	12	0.28231677171465464
1108	13	0.08212613769514832
1108	14	0.6443194148384634
1108	15	0.39647836155097593
1108	16	0.6941511624937888
1108	17	0.8182287222973414
1108	18	0.7167048095347468
1108	19	0.551105396304834
1108	20	0.3093211769259412
1108	21	0.419828067492368
1108	22	0.8261613698617651
1108	23	2.6200010099891315
1108	24	1.368602349323659
1108	25	1.415313539278877
1109	1	0.2961433411213896
1109	2	0.695146848235769
1109	3	0.5161026015417569
1109	4	0.467231577452381
1109	5	0.39384043722591855
1109	6	0.22327232871003844
1109	7	0.2797542971643736
1109	8	0.2543869927789686
1109	9	0.2342100865576029
1109	10	0.6961820377644514
1109	11	0.3400069278155655
1109	12	0.41851906204256295
1109	13	0.33023142868934485
1109	14	-0.722787040721748
1109	15	-0.03032424517296306
1109	16	-0.13212174054200432
1109	17	0.7421384932477366
1109	18	1.4837346300510816
1109	19	0.7248599198028671
1109	20	0.49135550556818686
1109	21	0.5398860383376061
1109	22	0.35354459630681845
1109	23	-4.200225669428217
1109	24	-1.4180508907276064
1109	25	-0.0714396660645562
1110	1	0.5078129509623786
1110	2	0.8926233006295874
1110	3	0.7260274527856758
1110	4	1.2258817233040986
1110	5	0.8929945546293396
1110	6	0.5456892491357763
1110	7	0.3861845715854457
1110	8	0.4761974340890305
1110	9	0.5308633091087529
1110	10	0.8338791053800065
1110	11	0.5157053170986075
1110	12	0.5433877877926179
1110	13	0.3415993271344604
1110	14	1.2473172312914258
1110	15	0.6863507586089099
1110	16	1.116327517503258
1110	17	1.3445056974707086
1110	18	2.012710079208709
1110	19	1.1586748824176922
1110	20	0.774999917602095
1110	21	0.7210516517996126
1110	22	0.88295298188374
1110	23	2.385522837574662
1110	24	1.458268240516162
1110	25	1.7851494815540931
1111	1	0.5344764105986783
1111	2	0.8089934679986506
1111	3	0.6022085910510242
1111	4	1.0764871020208773
1111	5	0.8818137695623989
1111	6	0.40199773346492407
1111	7	0.3183661984559123
1111	8	0.1503387634540161
1111	9	0.38519916774079377
1111	10	0.4790547255284565
1111	11	0.3289106757141198
1111	12	0.4854536847160702
1111	13	0.35822331076619207
1111	14	-0.211244960907905
1111	15	0.13205661262504975
1111	16	0.5856560529312418
1111	17	1.1198059874289825
1111	18	1.807474370027407
1111	19	0.8189033824922731
1111	20	0.5488510873432091
1111	21	0.42472682805903617
1111	22	0.776620797186311
1111	23	0.7795562677621035
1111	24	0.6903932549670984
1111	25	0.12405199594084593
1112	1	0.20948069604628117
1112	2	0.5561671496025262
1112	3	0.3542648005247173
1112	4	0.8589804169769479
1112	5	0.5713550054024097
1112	6	-0.00960836073828375
1112	7	0.059486081288127934
1112	8	0.08884212407574665
1112	9	0.0635027815727085
1112	10	0.40423776203979145
1112	11	0.01933757072549014
1112	12	0.17155443944655246
1112	13	-0.008951466148921838
1112	14	0.35088750155691173
1112	15	0.15704293395104685
1112	16	0.5124734938806554
1112	17	0.7414377570161932
1112	18	0.6782211162663501
1112	19	0.268478457684715
1112	20	0.1561808219743881
1112	21	0.09816614094961551
1112	22	0.07108318502923794
1112	23	1.7597008691917395
1112	24	0.6962811463013665
1112	25	0.7169527853279192
1113	1	0.38348588702196906
1113	2	0.796284330503422
1113	3	0.4854271644151851
1113	4	0.5984045719076079
1113	5	0.7565254014830671
1113	6	0.2830808871845534
1113	7	0.2634622458021241
1113	8	0.2096340550265552
1113	9	0.310780590919542
1113	10	0.598114176887826
1113	11	0.17892935731188686
1113	12	0.30325625014487656
1113	13	0.13628642797360158
1113	14	0.7506713388858657
1113	15	0.38215351094795835
1113	16	0.774805900619711
1113	17	1.1644869177060713
1113	18	1.7408334210288388
1113	19	0.7482232859064197
1113	20	0.4920008579119113
1113	21	0.49035740843844167
1113	22	0.711734821415213
1113	23	0.4574052446620698
1113	24	0.44209803386606694
1113	25	0.8792763612651413
1114	1	-0.4223161355660565
1114	2	-3.0979985365520157
1114	3	-1.1646448072390534
1114	4	-0.0035571170272135078
1114	5	0.16285890705625405
1114	6	-0.018448971824817306
1114	7	-0.09080807234949617
1114	8	-0.12978980265411622
1114	9	0.12352141914718828
1114	10	0.23750112602785833
1114	11	-0.07523023521783045
1114	12	0.1448669388582431
1114	13	0.018398703754222367
1114	14	0.7697622815299923
1114	15	0.07681099969085636
1114	16	0.43624016650883135
1114	17	0.8973871908292708
1114	18	1.0761973392395134
1114	19	0.5084155794031346
1114	20	0.22520009021426546
1114	21	0.08559989755042352
1114	22	0.3808829722973286
1114	23	1.9440904886275234
1114	24	0.9489464135811332
1114	25	0.7054426711492425
1115	1	0.10518248817026077
1115	2	0.33263719028055405
1115	3	0.169765675318871
1115	4	0.26937571546933725
1115	5	0.13075646246181094
1115	6	-0.17295232057100662
1115	7	-0.18571716113649472
1115	8	-0.30245671960948783
1115	9	-0.3327865371158296
1115	10	-0.024896626820471113
1115	11	-0.13147127491452437
1115	12	-0.36740933318872493
1115	13	-0.4524064153631002
1115	14	0.341085031411815
1115	15	-0.25887050857742366
1115	16	0.17912532095188283
1115	17	0.8130629800654248
1115	18	1.2650425032531842
1115	19	0.280002105052219
1115	20	-0.16950299670140367
1115	21	-0.17499344230598268
1115	22	0.18472094861586186
1115	23	1.8293989953960192
1115	24	0.5939349657906448
1115	25	0.18202751415792306
1116	1	0.14611352872393013
1116	2	0.10714120696783525
1116	3	0.19250846822187384
1116	4	0.8886228651086157
1116	5	0.4150256530811305
1116	6	0.20158200646940827
1116	7	0.11316863179025916
1116	8	0.14534662802575582
1116	9	0.0035099976643593322
1116	10	0.5249052814499962
1116	11	0.19994386043213552
1116	12	0.33970127207962264
1116	13	0.1439981886438204
1116	14	0.7945822769019273
1116	15	0.1578450479851199
1116	16	0.5595040420283937
1116	17	0.7582819931015194
1116	18	1.2570052833459116
1116	19	0.5390007550351186
1116	20	0.2936621204552736
1116	21	0.14963873364185076
1116	22	0.5121094817267249
1116	23	1.7535987587189084
1116	24	0.8048239978536458
1116	25	1.0594795896819704
1201	1	1.0697123394354868
1201	2	1.4455517271924876
1201	3	1.1556517634999963
1201	4	1.6848635350712016
1201	5	1.4999629074220948
1201	6	0.9589207273463956
1201	7	0.8505349085350176
1201	8	0.9942010462075785
1201	9	1.2420919916714297
1201	10	1.5716628016024927
1201	11	1.2377132414468122
1201	12	1.3651020332061705
1201	13	1.2187006930439566
1201	14	2.0707757144450127
1201	15	1.580820511490657
1201	16	1.9966853504914965
1201	17	2.3876092521822727
1201	18	2.8802187149042666
1201	19	2.0167607082943206
1201	20	1.7350897648984613
1201	21	1.7406586711374032
1201	22	1.9874772967106153
1201	23	3.599929688821831
1201	24	2.5014864670677497
1201	25	2.602414797106391
1202	1	1.0657017283029333
1202	2	1.3586696256266144
1202	3	1.0806306390684242
1202	4	1.737250433722707
1202	5	1.4482007900900769
1202	6	1.1324179323240031
1202	7	0.9787840556869014
1202	8	0.9247439339960835
1202	9	1.1647155318865074
1202	10	1.5772476774586863
1202	11	1.1827799454048815
1202	12	1.2531524608026317
1202	13	1.1978801939011987
1202	14	2.029641552148668
1202	15	1.4617674348702678
1202	16	1.8278267332975844
1202	17	2.2595375399177584
1202	18	2.8791781645478594
1202	19	2.0796478426955147
1202	20	1.7337560898525979
1202	21	1.6926590568327313
1202	22	2.0312642421455323
1202	23	3.6521194167139726
1202	24	2.2789674235959154
1202	25	2.545606123322216
1203	1	1.0138648394787104
1203	2	1.2429258995144359
1203	3	1.0201314342204029
1203	4	1.6306632690438718
1203	5	1.425849065128311
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2211	14	-0.08473298996447909
2211	15	-0.40618733113878847
2211	16	-0.6225470489186904
2211	17	-0.13561748195620282
2211	18	-5.254125030682516
2211	19	-1.8353811570628693
2211	20	-1.1495031650123764
2211	21	-0.9477600058087106
2211	22	-0.6181269531674687
2211	23	-5.016555750592669
2211	24	-2.271359191694728
2211	25	-1.6583914038349619
2212	1	0.044382090295773224
2212	2	0.28730051211874397
2212	3	0.01827220588823053
2212	4	0.5154050230143881
2212	5	0.40780245534079995
2212	6	0.07160475401301625
2212	7	-0.0636400356336253
2212	8	-0.14536615245579299
2212	9	-0.4319292490312563
2212	10	-3.991514600378533
2212	11	-1.385486549757438
2212	12	-0.6158206140338294
2212	13	-0.3540138558756354
2212	14	0.45498966566788823
2212	15	-0.04486750099030701
2212	16	0.09574043458423631
2212	17	0.7333506439685777
2212	18	1.2293351340968144
2212	19	0.5575005746877153
2212	20	0.11091284055299866
2212	21	-0.01590571398350704
2212	22	0.17185955846480283
2212	23	1.4261581519248239
2212	24	0.5511925039260248
2212	25	0.4186417028781335
2213	1	-1.4379371797855782
2213	2	-3.9160379205989315
2213	3	-1.71111789416382
2213	4	-3.264535459298674
2213	5	-1.213413464898558
2213	6	-0.9746912512065791
2213	7	-0.7972053771226228
2213	8	-0.7396545066707816
2213	9	-0.6593156841994556
2213	10	-0.7352436695770024
2213	11	-0.7864876151540514
2213	12	-0.6527437446081663
2213	13	-1.004450346577073
2213	14	-6.010941408729558
2213	15	-2.588924027284328
2213	16	-1.7884637215271795
2213	17	-0.623914419481153
2213	18	0.0948860893364909
2213	19	-0.644832316106573
2213	20	-0.6015643712850505
2213	21	-0.8172564849814137
2213	22	-0.7659178929344961
2213	23	-5.6188560912265295
2213	24	-2.9610131990181934
2213	25	-2.0623039273569006
2214	1	-0.08870582270915861
2214	2	0.036133007148421825
2214	3	-0.14234632444385653
2214	4	0.13704395590011842
2214	5	-0.10754048489371115
2214	6	-0.3378578752116326
2214	7	-0.3995403464360976
2214	8	-0.35311488290266624
2214	9	-0.39636538441492064
2214	10	0.06563409514154017
2214	11	-0.3729353672579734
2214	12	-0.3022960073430596
2214	13	-0.3807657799360058
2214	14	0.24486214332962675
2214	15	-0.273277279639629
2214	16	0.19909110348520898
2214	17	0.4013818206677584
2214	18	1.021852551824189
2214	19	0.014744619395997915
2214	20	-0.034205150981279075
2214	21	-0.18681267355252373
2214	22	0.23081829200109638
2214	23	1.7562394135297197
2214	24	0.7890067608105298
2214	25	0.643850296715442
2215	1	-2.5163189189126385
2215	2	-4.454299607687023
2215	3	-2.501162570160087
2215	4	-4.095586444865678
2215	5	-5.085326937715784
2215	6	-2.513621442814184
2215	7	-1.8078152035609172
2215	8	-2.0365531953295957
2215	9	-1.6778150677671633
2215	10	-3.1810466119314142
2215	11	-2.1182009674488
2215	12	-1.7856582191922072
2215	13	-1.868459769156594
2215	14	-1.0987414507199702
2215	15	-1.7418344770609195
2215	16	-2.4440205891182174
2215	17	-7.079895166931141
2215	18	-3.303876658560011
2215	19	-2.324502706393311
2215	20	-2.085181575507434
2215	21	-2.05428477687515
2215	22	-1.7786836286288263
2215	23	-6.421514602470687
2215	24	-3.589048049954201
2215	25	-3.2641983112457567
2216	1	-0.927385348278932
2216	2	-0.4015570708037828
2216	3	-0.7580055472899581
2216	4	-2.892892638674798
2216	5	-1.2598905740946407
2216	6	-1.0355033767214081
2216	7	-1.113554695146874
2216	8	-0.9366793616681051
2216	9	-0.891332285298283
2216	10	-0.5788325102651939
2216	11	-1.1418057693340131
2216	12	-1.9277569485566204
2216	13	-1.2003334774051382
2216	14	-7.06968400131645
2216	15	-3.3535711351361357
2216	16	-1.7433338847398971
2216	17	-0.898862185837217
2216	18	-0.09156684505013146
2216	19	-0.6865611346722815
2216	20	-1.2012506497323443
2216	21	-1.2366458567323804
2216	22	-0.7989630087996886
2216	23	0.4009167969133145
2216	24	-0.380011681797491
2216	25	-0.36935881813018767
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95352.0
3	3.0
4	863.0
5	4982.0
6	5985.0
7	5432.0
8	5023.0
9	4699.0
10	4460.0
11	4422.0
12	4331.0
13	4362.0
14	4369.0
15	4545.0
16	4549.0
17	4986.0
18	5505.0
19	6527.0
20	7920.0
21	10064.0
22	12576.0
23	15286.0
24	18010.0
25	21414.0
26	24559.0
27	27928.0
28	31475.0
29	37843.0
30	44682.0
31	50502.0
32	66349.0
33	86890.0
34	113945.0
35	151403.0
36	213764.0
37	301800.0
38	768429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46948024932716	18.08095953403949	12.14024836991138	25.309311846721965
2	13.78885301395443	19.705457589388377	41.966544323941456	24.539145072715733
3	19.51166345670973	24.14940739964056	28.78448404311092	27.554445100538793
4	10.63110640211684	16.257572789370915	39.22808363247403	33.883237176038214
5	12.333156126867566	37.98199852103116	36.28192015775667	13.4029251943446
6	32.621875099145726	36.161141864486815	18.142207962097253	13.074775074270207
7	28.749494206363803	30.995355894794923	21.57591502666912	18.679234872172152
8	25.642321411081564	36.6613955118086	20.277928882173367	17.418354194936473
9	26.71288783266203	15.238775379756527	19.655047302582478	38.39328948499897
10	14.039606637712671	29.425463696214827	34.95422362270773	21.58070604336477
11	34.60198447932822	22.800176753754524	23.385616088454125	19.212222678463124
12	23.20708475682933	25.06559193676689	31.61779649716527	20.10952680923851
13	29.078637674669032	21.386970153556558	25.21537781754463	24.319014354229783
14	20.452568250330607	22.025779064442144	28.327450767615048	29.1942019176122
15	24.27692586412308	29.584964343470617	22.906992303118894	23.231117489287406
16	21.821668088276205	28.164974146631934	28.257351943773234	21.756005821318624
17	22.020221714155383	28.116579267316933	28.258806304524963	21.604392714002717
18	21.585682900204983	27.222896103454207	30.081138570110877	21.110282426229933
19	23.260075786840314	27.316097345621102	27.82563508268491	21.598191784853668
20	23.331549213653847	27.281902864799594	28.083331264122364	21.30321665742419
21	22.869106808152935	26.789225559497993	27.652453661039957	22.689213971309115
22	22.63680299329626	27.669595057897162	27.10734026256646	22.586261686240118
23	20.872253944254144	27.234471877602466	29.32932159610488	22.56395258203851
24	22.5207633040822	27.842994054881625	27.70341461440432	21.93282802663185
25	22.45675688850097	26.918980614982484	27.47490763674223	23.14935485977432
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189.0
1	189.0
2	315.0
3	441.0
4	441.0
5	441.0
6	997.0
7	1553.0
8	1553.0
9	1553.0
10	2506.0
11	3459.0
12	3459.0
13	3459.0
14	6230.5
15	9002.0
16	9002.0
17	9002.0
18	16185.0
19	23368.0
20	23368.0
21	23368.0
22	36908.5
23	50449.0
24	50449.0
25	50449.0
26	72341.5
27	94234.0
28	94234.0
29	94234.0
30	121166.5
31	148099.0
32	148099.0
33	148099.0
34	172707.5
35	197316.0
36	197316.0
37	197316.0
38	218323.0
39	239330.0
40	239330.0
41	239330.0
42	256769.0
43	274208.0
44	274208.0
45	274208.0
46	296945.0
47	319682.0
48	319682.0
49	319682.0
50	301619.5
51	283557.0
52	283557.0
53	283557.0
54	246800.0
55	210043.0
56	210043.0
57	210043.0
58	177542.5
59	145042.0
60	145042.0
61	145042.0
62	117331.5
63	89621.0
64	89621.0
65	89621.0
66	69177.0
67	48733.0
68	48733.0
69	48733.0
70	35556.5
71	22380.0
72	22380.0
73	22380.0
74	15704.0
75	9028.0
76	9028.0
77	9028.0
78	6310.0
79	3592.0
80	3592.0
81	3592.0
82	2448.0
83	1304.0
84	1304.0
85	1304.0
86	870.5
87	437.0
88	437.0
89	437.0
90	285.5
91	134.0
92	134.0
93	134.0
94	80.0
95	26.0
96	26.0
97	26.0
98	16.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10785046574299594
2	0.046615674451576244
3	0.03447904915057415
4	0.030893228038914436
5	0.03429516088843775
6	0.018618686541310037
7	0.019951876441798903
8	0.023215893094719924
9	0.028364764434538996
10	0.03447904915057415
11	0.02100923394908318
12	0.019492155786457917
13	0.015400641953923117
14	0.02050354122820809
15	0.02100923394908318
16	0.021560898735492363
17	0.024916859519481582
18	0.0200438205728671
19	0.03282405479134659
20	0.02261825624277664
21	0.02785907171366391
22	0.035628350788926615
23	0.028272820303470798
24	0.03884639537631354
25	0.03070933977677804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2175234.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.43217797404075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.3591038012986	65.14255287618215
2	9.61204623599275	14.501151647362272
3	2.2385989049965764	5.065871730425835
4	0.7513490369558904	2.267035771051234
5	0.35901840177190864	1.3540769989207142
6	0.16576077611016754	0.7502217818794369
7	0.11471423237947802	0.6057201075102978
8	0.0760498059180177	0.45892819959193354
9	0.05127801801950452	0.34812113232630004
>10	0.24237936293718645	3.1612565897024028
>50	0.015996716517977138	0.8417870647015192
>100	0.010373755038854328	1.5158575843374478
>500	0.0019122132214234424	1.0408252985507966
>1k	0.00129537024677031	2.0037131669490282
>5k	6.168429746525286E-5	0.42611999124350836
>10k+	6.168429746525286E-5	0.5167600592651674
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11106	0.5105657598217019	No Hit
CCCATGTACTCTGCGTTGATACCAC	9158	0.42101217616127734	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4478	0.2058629094616947	No Hit
GTATCAACGCAGAGTACATGGGAAG	3074	0.1413181294518199	No Hit
GCGTTGATACCACTGCTTCCCATGT	3049	0.14016882781346743	No Hit
CATGTACTCTGCGTTGATACCACTG	2897	0.1331810738522844	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2529	0.11626335373573601	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2494	0.11465433144204255	No Hit
TATCAACGCAGAGTACATGGGAAGC	2308	0.10610352725270017	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2248	0.10334520332065424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.597206553409886E-5
7	0.0	0.0	0.0	0.0	4.597206553409886E-5
8	0.0	0.0	0.0	0.0	4.597206553409886E-5
9	0.0	0.0	0.0	0.0	4.597206553409886E-5
10	0.0	0.0	0.0	4.597206553409886E-5	4.597206553409886E-5
11	0.0	0.0	0.0	9.194413106819772E-5	4.597206553409886E-5
12	0.0	0.0	0.0	1.379161966022966E-4	1.8388826213639543E-4
13	0.0	0.0	0.0	1.379161966022966E-4	1.8388826213639543E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCC	40	0.0052845804	14.248137	8
GCGTTTA	190	0.0	13.997848	13
TAGGACC	735	0.0	12.668507	4
CGTTTAT	220	0.0	12.520802	14
TGTAGGA	1520	0.0	12.439879	2
GTAGGAC	1400	0.0	12.41909	3
GTCCTAC	1465	0.0	12.206377	1
GACCCGT	55	0.0030716006	12.089606	6
AAGTCGT	55	0.0030721298	12.089329	7
GGACCGA	65	8.032941E-4	11.691048	6
GTGTAGG	440	0.0	11.673676	1
CTGTAGG	1520	0.0	11.576964	1
AGGACGT	1020	0.0	11.36309	5
TCCTACA	1635	0.0	11.332443	2
TAGGACA	580	0.0	11.303366	4
GGCGAGG	420	0.0	11.082905	19
TGGCGAG	1115	0.0	11.075804	18
TATGGCG	1185	0.0	11.062354	16
GGACGTG	990	0.0	11.034164	6
TAGGACG	1070	0.0	11.010945	4
>>END_MODULE