FastQCFastQC Report
Thu 26 May 2016
SRR1513115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175234
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC129350.5946486676835687No Hit
CCCATGTACTCTGCGTTGATACCAC117600.5406314906810026No Hit
GAGTACATGGGAAGCAGTGGTATCA48720.22397590328212966No Hit
GTATCAACGCAGAGTACTTTTTTTT36990.17005067041063168No Hit
CATGTACTCTGCGTTGATACCACTG35500.16320083264605095No Hit
GTATCAACGCAGAGTACATGGGAAG33380.153454754752822No Hit
GCGTTGATACCACTGCTTCCCATGT32250.14825991134746883No Hit
ACGCAGAGTACATGGGAAGCAGTGG29810.1370427273571487No Hit
GCTTCCCATGTACTCTGCGTTGATA28990.1332730179833526No Hit
TATCAACGCAGAGTACATGGGAAGC28310.1301469175270339No Hit
TATCAACGCAGAGTACTTTTTTTTT25600.11768848776729308No Hit
ACTCTGCGTTGATACCACTGCTTCC23890.10982726456096217No Hit
GTCCTACAGTGGACATTTCTAAATT23150.10642533171143886No Hit
GGTATCAACGCAGAGTACTTTTTTT22770.1046783932211431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7050.013.0977924
GGACCGT604.0236706E-412.6924126
AGGACGT11050.011.8886435
TGTAGGA15500.011.7995542
GGACGTG10900.011.7026376
GTAGGAC14100.011.679993
ACCCCGC658.11742E-411.6764768
CCAACGA907.5641456E-611.59725519
TAGGACG11700.011.390894
GCACCGT855.2158786E-511.1991866
TGGCGAG12550.011.11651118
CTGTAGG15050.010.9525691
GTACACG700.00145550110.889261
TCCAACG1752.1827873E-1110.84643218
TTAGGAC8000.010.82846453
GTCCTAC13300.010.8176191
GACGTGA6250.010.7776357
AGGACCT10200.010.7328035
GACGTGG5750.010.5598387
GGACCTG10050.010.51386456
Produced by FastQC (version 0.11.5)