FastQCFastQC Report
Thu 26 May 2016
SRR1513114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC90130.5233103312131127No Hit
CCCATGTACTCTGCGTTGATACCAC71860.41723155887023494No Hit
GAGTACATGGGAAGCAGTGGTATCA37260.2163379889160166No Hit
GTATCAACGCAGAGTACATGGGAAG26120.15165722679780874No Hit
GCGTTGATACCACTGCTTCCCATGT23410.135922499208909No Hit
GTCCTACAGTGGACATTTCTAAATT22790.13232267223285074No Hit
CATGTACTCTGCGTTGATACCACTG22690.1317420549786478No Hit
GTATCAACGCAGAGTACTTTTTTTT21800.1265745614162416No Hit
ACGCAGAGTACATGGGAAGCAGTGG19440.11287199421705216No Hit
TATCAACGCAGAGTACATGGGAAGC18360.10660132787166036No Hit
GCTTCCCATGTACTCTGCGTTGATA18110.10514978473615301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6150.014.058934
TGCGTTT2150.013.25425412
GCGTTTA2100.013.11750313
CGTTTAT2150.012.37063814
AGGACCT10650.011.7756565
GTAGGAC13450.011.5139973
TGTAGGA14850.011.4524912
TGGCGAG10350.011.19772518
CTGTAGG14250.011.143351
GTCCTAC13750.011.0644911
GGACCTG10750.011.0458536
GTGTAGG2600.010.9714011
GGCGAGA7450.010.96610719
ACTGTCG700.001493008710.85586558
ATGGCGA11650.010.76299517
TCCTACA15450.010.7002552
GTCTAGG1701.364242E-1010.6271991
TAGGACT3400.010.6191734
TCTTAGG2150.010.6058682
ACCGTGC909.526886E-510.5543138
Produced by FastQC (version 0.11.5)