##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513114_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1722305
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.69005431674413	31.0	30.0	34.0	25.0	34.0
2	29.408003808849188	31.0	30.0	34.0	23.0	34.0
3	29.776003669501048	31.0	30.0	34.0	25.0	34.0
4	32.65265269508014	37.0	33.0	37.0	22.0	37.0
5	32.88202263826674	37.0	33.0	37.0	25.0	37.0
6	33.330126197160205	37.0	35.0	37.0	28.0	37.0
7	33.32414293635564	37.0	35.0	37.0	28.0	37.0
8	33.314793837328466	37.0	35.0	37.0	28.0	37.0
9	34.73800633453424	39.0	35.0	39.0	27.0	39.0
10	34.21942048591858	39.0	35.0	39.0	25.0	39.0
11	34.62159141383204	39.0	35.0	39.0	27.0	39.0
12	34.558826108035454	39.0	35.0	39.0	27.0	39.0
13	34.59360333971045	39.0	35.0	39.0	27.0	39.0
14	35.08458141850601	40.0	35.0	41.0	22.0	41.0
15	35.542410897024624	40.0	36.0	41.0	27.0	41.0
16	35.09201389997707	40.0	35.0	41.0	23.0	41.0
17	34.60059164898203	40.0	34.0	41.0	17.0	41.0
18	33.96293397510894	39.0	33.0	41.0	15.0	41.0
19	34.90637837084605	40.0	34.0	41.0	22.0	41.0
20	35.20426173064585	40.0	35.0	41.0	25.0	41.0
21	35.24509421966493	40.0	36.0	41.0	25.0	41.0
22	34.842540664980945	40.0	35.0	41.0	18.0	41.0
23	33.05241580324042	39.0	32.0	41.0	10.0	41.0
24	34.236157358888235	39.0	34.0	41.0	17.0	41.0
25	33.98672418648265	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5228769883605118
1101	2	0.9288148801096057
1101	3	0.6247189757978582
1101	4	0.5835673274499626
1101	5	0.8560846194169471
1101	6	0.5309388988327655
1101	7	0.4529483032697783
1101	8	0.38505637676252036
1101	9	0.691419186256006
1101	10	0.8415423384848282
1101	11	0.5159711465255441
1101	12	0.6293623977614473
1101	13	0.5822703524389183
1101	14	-0.3007587747201157
1101	15	0.06213303689364125
1101	16	0.9265036947675043
1101	17	1.4602926081805947
1101	18	-4.534909140315911
1101	19	-0.9814139912307169
1101	20	-0.12764814839259486
1101	21	0.36039111904996446
1101	22	0.9210371443583512
1101	23	2.7927977863025077
1101	24	1.5934955639449981
1101	25	1.7954428528946025
1102	1	0.8044766672383119
1102	2	1.1046788384813198
1102	3	0.7766199248676031
1102	4	1.3031001354269947
1102	5	1.0974862903240634
1102	6	0.8082908925336127
1102	7	0.600137689735277
1102	8	0.5716372907136744
1102	9	0.6764238416269706
1102	10	1.0046511494530606
1102	11	0.7641024187399168
1102	12	0.7187379331528163
1102	13	0.605573761943937
1102	14	1.5155853590264314
1102	15	1.0500626214145257
1102	16	1.2989119144905814
1102	17	0.9341351610926125
1102	18	1.2842705375613406
1102	19	1.026622243606731
1102	20	0.9301741195732589
1102	21	0.9343971730475289
1102	22	1.3275764527838518
1102	23	2.9483458575421295
1102	24	1.7168205728538624
1102	25	2.0226651434793723
1103	1	0.6236724956787683
1103	2	0.9552390859534015
1103	3	0.7725342122524026
1103	4	1.175846272021758
1103	5	1.102707431867472
1103	6	0.7195773918598221
1103	7	0.6568565384659948
1103	8	0.6166158207635064
1103	9	0.7707044779555758
1103	10	1.1138937123407473
1103	11	0.690084803683078
1103	12	0.8770598840195376
1103	13	0.6751586136243546
1103	14	1.4791483164530703
1103	15	0.9238011869959664
1103	16	1.0909247220255764
1103	17	1.6727243410454804
1103	18	1.6572011182514643
1103	19	1.0581172175931854
1103	20	1.0738890608129665
1103	21	1.0981200133316236
1103	22	1.3965001938971326
1103	23	2.919130546827205
1103	24	1.7538536833280105
1103	25	1.9338921181993811
1104	1	0.3309806293398587
1104	2	0.7305031121365282
1104	3	0.5587659466441508
1104	4	1.1177138361662458
1104	5	0.8938903937916507
1104	6	0.503678153208341
1104	7	0.3521098899467887
1104	8	0.28725971255367
1104	9	0.4406879104628203
1104	10	0.8076588133455118
1104	11	0.4435152828754596
1104	12	0.5035259344286871
1104	13	0.42109299664452493
1104	14	1.2493927353970946
1104	15	0.6080752780651295
1104	16	1.0404567644527347
1104	17	1.416805587339148
1104	18	2.015511598059966
1104	19	1.1941578272041085
1104	20	0.6314634902566638
1104	21	0.759517358472003
1104	22	1.041252098085259
1104	23	2.4780873898971834
1104	24	1.4926750598978558
1104	25	1.462808099143615
1105	1	0.3061314679934348
1105	2	0.7688436696062588
1105	3	0.4416145635428528
1105	4	0.6770273339384687
1105	5	0.6103495834454336
1105	6	0.27062362737999024
1105	7	0.20804996795201447
1105	8	0.07735539906800426
1105	9	0.25004104269178384
1105	10	0.7441331096364507
1105	11	0.31406436220882483
1105	12	0.31750249089253657
1105	13	0.2935419932960954
1105	14	1.0139037603481853
1105	15	0.3621218840354459
1105	16	0.7639116807338056
1105	17	1.2002568670507188
1105	18	0.9642792057034058
1105	19	0.6885026387914124
1105	20	0.37830256306288135
1105	21	0.26472310655781683
1105	22	0.7868362431034441
1105	23	2.145068019111335
1105	24	1.1362536610500058
1105	25	1.159349864521694
1106	1	-0.6563980221460355
1106	2	-0.3203729740534307
1106	3	-0.44100668334371207
1106	4	-2.8256802821736215
1106	5	-0.6242285765886919
1106	6	-0.6571823942516772
1106	7	-0.7238441478635309
1106	8	-0.509328812128075
1106	9	-0.5454225927863661
1106	10	-0.4127249971979836
1106	11	-0.4731673068084916
1106	12	-0.46291973143275555
1106	13	-0.45139192274586293
1106	14	0.14412967113962338
1106	15	-0.4747073323632165
1106	16	-1.477675371327372
1106	17	-6.499086366715744
1106	18	-7.771245219571135
1106	19	-3.5439442985809713
1106	20	-2.111592028234142
1106	21	-1.6840420390081405
1106	22	-1.9478757724611384
1106	23	-5.875659429502356
1106	24	-2.913112805659928
1106	25	-1.474685785172376
1107	1	0.3090058265014548
1107	2	0.6547075191116463
1107	3	0.38150211958691216
1107	4	0.6473190398129134
1107	5	0.743439894445487
1107	6	0.20497201386457675
1107	7	-0.008483721743786532
1107	8	0.19307303980086488
1107	9	0.12676387862008198
1107	10	0.6936143808383051
1107	11	0.2649551365855203
1107	12	0.33516180626541114
1107	13	0.4062508488506822
1107	14	1.067265217849588
1107	15	0.432300513357859
1107	16	-6.483781320381144
1107	17	-1.4253945092411584
1107	18	0.27338344963245476
1107	19	0.2822948942337149
1107	20	0.214284233591016
1107	21	0.26923233059963536
1107	22	0.2779601291412561
1107	23	2.2846345326370283
1107	24	1.2895336718041435
1107	25	0.9197264173474196
1108	1	0.19088901905496414
1108	2	0.4877602578643412
1108	3	0.3115078858899878
1108	4	0.8392867485156899
1108	5	0.5637377638085681
1108	6	0.10503956887968968
1108	7	0.1643873905254054
1108	8	0.17564336336526054
1108	9	0.26877706632410536
1108	10	0.7319746517339141
1108	11	0.32995584832585934
1108	12	0.3115142938582096
1108	13	0.15554912113117325
1108	14	0.8329319496585867
1108	15	0.42087165240779
1108	16	0.6537558650356559
1108	17	0.8202819697914521
1108	18	0.6330208963424084
1108	19	0.5315018466098422
1108	20	0.3383295967836659
1108	21	0.42346585863617037
1108	22	0.8385615617901152
1108	23	2.549836220547334
1108	24	1.2599224991697113
1108	25	1.4851336655180063
1109	1	0.2787197068856493
1109	2	0.4274427975468811
1109	3	0.3178570922391941
1109	4	0.3119498878454934
1109	5	0.07837621178034482
1109	6	0.1829937135005011
1109	7	0.2169447097493915
1109	8	0.09416188188377816
1109	9	0.0680715989519669
1109	10	0.616278002703929
1109	11	0.25447083950751903
1109	12	0.2624843114948945
1109	13	0.1781240770394561
1109	14	-0.8919357752091344
1109	15	-0.21792905305957788
1109	16	-0.46934819140172834
1109	17	0.6241620403381916
1109	18	1.401274864596374
1109	19	0.5501967319713899
1109	20	0.4000579918454008
1109	21	0.3532718551088365
1109	22	0.2491435723721267
1109	23	-4.3746787706343255
1109	24	-1.4342573950101851
1109	25	-0.1684818547641811
1110	1	0.3925077726258799
1110	2	0.7533889470362354
1110	3	0.5547440034372784
1110	4	1.1844455314045703
1110	5	0.8657197350600896
1110	6	0.4077488439890118
1110	7	0.4127806408180632
1110	8	0.2875749556110776
1110	9	0.30186455308728455
1110	10	0.5741957449105684
1110	11	0.2703792155988012
1110	12	0.31701237355022016
1110	13	0.18096306831789377
1110	14	1.009824233128434
1110	15	0.5685489513337743
1110	16	0.9683575880088213
1110	17	1.1409439378915707
1110	18	1.8330681673710387
1110	19	1.0212778427288995
1110	20	0.588731059331927
1110	21	0.5085444048473207
1110	22	0.849415784017296
1110	23	2.205775713630686
1110	24	1.2353348644519357
1110	25	1.529204036074482
1111	1	0.3642852419173792
1111	2	0.6964097648531116
1111	3	0.4636428033867084
1111	4	0.9864771180073646
1111	5	0.6928583773894417
1111	6	0.22767917170909158
1111	7	0.11560846416641368
1111	8	-0.09061722021441909
1111	9	-0.004251987131993928
1111	10	0.2702814045127937
1111	11	0.04029433458368459
1111	12	0.2766043288177613
1111	13	0.028489677345746145
1111	14	-0.6247023496127184
1111	15	-0.1967957862560894
1111	16	0.25890188548417115
1111	17	0.7941231894215193
1111	18	1.3459942153655504
1111	19	0.4176749371720163
1111	20	0.26709810431848524
1111	21	0.2725780618409033
1111	22	0.578443063356076
1111	23	0.5616289487101724
1111	24	0.3414868291326201
1111	25	0.06719320344943469
1112	1	0.5520453821850104
1112	2	0.8325585963030306
1112	3	0.602420352241051
1112	4	1.1992851240842413
1112	5	1.0271451248036456
1112	6	0.37040669038716345
1112	7	0.3128697074287743
1112	8	0.34624231444666975
1112	9	0.33279823034526856
1112	10	0.78227517155198
1112	11	0.3479684724788328
1112	12	0.42584758077921947
1112	13	0.3229955148933499
1112	14	0.7727815737187385
1112	15	0.5171888342512929
1112	16	1.0043197748100923
1112	17	1.0847394096874865
1112	18	1.3074221773226355
1112	19	0.6828686896433496
1112	20	0.685129466829153
1112	21	0.6684881365531865
1112	22	0.7020942360684046
1112	23	2.350240007793225
1112	24	1.2625888873624191
1112	25	1.3728842920844215
1113	1	0.6105701430839119
1113	2	1.0112524393275386
1113	3	0.6569686516406108
1113	4	0.8325399794500825
1113	5	1.0235573859470293
1113	6	0.5320761930177653
1113	7	0.3920811138713063
1113	8	0.35847974330309285
1113	9	0.526220749854744
1113	10	0.8498407898899103
1113	11	0.4930084693494976
1113	12	0.49830034731093065
1113	13	0.37014097050563066
1113	14	1.1223092390358786
1113	15	0.5894936905950487
1113	16	0.9676062658425835
1113	17	1.432487263156169
1113	18	2.020527753414484
1113	19	1.074869804470552
1113	20	0.8581941659235994
1113	21	0.7739639917430026
1113	22	1.0516964676786458
1113	23	0.9786553884969393
1113	24	0.8534741970692323
1113	25	1.2895717395212998
1114	1	-0.19307205209540257
1114	2	-2.96941564698524
1114	3	-0.8903306457127229
1114	4	0.3123741124889676
1114	5	0.4640416003775414
1114	6	0.17556809613224544
1114	7	0.1372343155711846
1114	8	0.20581861737735352
1114	9	0.28336122163483424
1114	10	0.5453778573614656
1114	11	0.3734931669428434
1114	12	0.4178800240607785
1114	13	0.3433403540466884
1114	14	1.2037231724574582
1114	15	0.557284492453519
1114	16	0.928956933353362
1114	17	1.146609189299042
1114	18	1.4077070274338936
1114	19	0.8038109033675909
1114	20	0.633927170137774
1114	21	0.43679150331936967
1114	22	0.5507565027932131
1114	23	2.199935869499754
1114	24	1.0772543352395942
1114	25	0.9726932272649478
1115	1	1.0862179470019164
1115	2	1.3952123246869377
1115	3	1.1459637601169632
1115	4	1.3287372863210223
1115	5	1.3743576213526012
1115	6	1.0009490692240988
1115	7	0.8884262669248031
1115	8	0.8492557045026388
1115	9	0.9231654215708289
1115	10	1.197883622220857
1115	11	1.1443906796419512
1115	12	0.7990853515294134
1115	13	0.7975456240718799
1115	14	1.8165207499855853
1115	15	1.0940737324768222
1115	16	1.6604933812152325
1115	17	2.1516018447450875
1115	18	2.63061382955766
1115	19	1.7586078408116848
1115	20	1.3608008520160126
1115	21	1.405142630441702
1115	22	1.7113314295528284
1115	23	3.4747198411391125
1115	24	2.200417991596538
1115	25	2.071856194300622
1116	1	1.1273969555628973
1116	2	1.0175662543370052
1116	3	1.1150352227506595
1116	4	1.9655654081276808
1116	5	1.3570358237732947
1116	6	1.1269090574158511
1116	7	1.0564508825888979
1116	8	0.8800525344410985
1116	9	1.1809463784934167
1116	10	1.6815337346263277
1116	11	1.2333068183434932
1116	12	1.1672462162567996
1116	13	1.090998856580903
1116	14	1.9884875052141453
1116	15	1.5520885836247231
1116	16	1.8495230608028521
1116	17	2.1423278251706392
1116	18	2.5854018487233574
1116	19	1.775593557368218
1116	20	1.4723683974891344
1116	21	1.5800796152499288
1116	22	1.9345051244003457
1116	23	3.4821113528224643
1116	24	2.46944630869352
1116	25	2.6152923956767395
1201	1	0.8037268704578366
1201	2	1.0846760586665383
1201	3	0.8562344321113038
1201	4	1.3837128809836727
1201	5	1.2268208801054357
1201	6	0.7027697355186788
1201	7	0.622288765018709
1201	8	0.6816013261244223
1201	9	0.7653420193912979
1201	10	1.19574717440031
1201	11	0.8028737735606768
1201	12	0.8172225810313734
1201	13	0.8244055594943376
1201	14	1.7212514030004655
1201	15	1.0499756492247911
1201	16	1.4941496516177892
1201	17	1.893802425972595
1201	18	2.4691282203421068
1201	19	1.507861024164832
1201	20	1.18004427915605
1201	21	1.0285112035918331
1201	22	1.4597648837408173
1201	23	3.056110049691121
1201	24	1.9324919493176367
1201	25	1.9864570556306376
1202	1	0.3997742589746842
1202	2	0.717624637843663
1202	3	0.40981902132756076
1202	4	0.9895719762721811
1202	5	0.7874773490424047
1202	6	0.37517579706189963
1202	7	0.22886285120776506
1202	8	0.2195398542732434
1202	9	0.2975875023759187
1202	10	0.8507783980089272
1202	11	0.428254820532878
1202	12	0.5464288471635683
1202	13	0.46358232675058275
1202	14	1.34550994637452
1202	15	0.7942779651244507
1202	16	1.044653511450548
1202	17	1.3350116419923879
1202	18	1.9834405277965246
1202	19	1.1573706053521633
1202	20	0.7457838326287103
1202	21	0.6208404253210844
1202	22	1.0920876823277297
1202	23	2.6781266784929656
1202	24	1.331746983752815
1202	25	1.5254347054742183
1203	1	0.6371563260390154
1203	2	0.9146735220852094
1203	3	0.6767671427182798
1203	4	1.252070609794842
1203	5	1.004805469957823
1203	6	0.6265921805310839
1203	7	0.4976142473911338
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2211	17	0.20406245227628972
2211	18	-5.70857238800706
2211	19	-1.7942121898226873
2211	20	-0.9536958798118889
2211	21	-0.674696311610802
2211	22	-0.3366280481462596
2211	23	-4.683298933789235
2211	24	-1.912360485480761
2211	25	-1.201679952744371
2212	1	0.23822316638910834
2212	2	0.5439805807513309
2212	3	0.15996806768350424
2212	4	0.8567741993364706
2212	5	0.6573207240581951
2212	6	0.16606249656928895
2212	7	0.11461124074925522
2212	8	-0.03659378220521603
2212	9	-0.09125549370845931
2212	10	-4.011506558413963
2212	11	-1.1180973330606534
2212	12	-0.2399169909064085
2212	13	-0.1157166501346012
2212	14	0.7710271877538304
2212	15	0.2940910256271678
2212	16	0.500031811311608
2212	17	0.9606021434449588
2212	18	1.483611046932559
2212	19	0.7427893245639865
2212	20	0.4698042949250336
2212	21	0.4374666726369867
2212	22	0.6445987344939539
2212	23	1.8907804614915733
2212	24	1.0369269761741862
2212	25	0.927868708253051
2213	1	-1.3341614788643277
2213	2	-3.6413160845305086
2213	3	-1.5290350468199811
2213	4	-3.4103073724152253
2213	5	-1.0106349180264331
2213	6	-0.704010573429656
2213	7	-0.6204965721198619
2213	8	-0.4671637064741816
2213	9	-0.4851739301028033
2213	10	-0.5209690010861934
2213	11	-0.7088431575351706
2213	12	-0.5316775407099499
2213	13	-0.849477922220558
2213	14	-5.633939454203176
2213	15	-2.460335669496345
2213	16	-1.685131258877412
2213	17	-0.5249714872732199
2213	18	0.3117360723889391
2213	19	-0.401010315380951
2213	20	-0.5696168473130072
2213	21	-0.6789399995146823
2213	22	-0.3619398220816663
2213	23	-5.580840628068728
2213	24	-2.842437469609827
2213	25	-2.078061882036323
2214	1	0.3066708106988223
2214	2	0.4998982263241487
2214	3	0.3773483885121003
2214	4	0.7631149849876024
2214	5	0.4625247154690797
2214	6	0.23093555454579473
2214	7	0.15928725439079017
2214	8	0.11977858106369865
2214	9	0.24275991616902104
2214	10	0.6786791179786107
2214	11	0.3260608076492062
2214	12	0.340328051407603
2214	13	0.23164977654217012
2214	14	0.9255175250027889
2214	15	0.4861460969925844
2214	16	0.9530695781424754
2214	17	1.1011730667744999
2214	18	1.5235125247650743
2214	19	0.8147105068299965
2214	20	0.6367993688166607
2214	21	0.3069374317629183
2214	22	0.9329023848665727
2214	23	2.409449149125777
2214	24	1.323815949270056
2214	25	1.1936764637159811
2215	1	-2.1010512846500475
2215	2	-4.1151641960405065
2215	3	-2.0310565939293923
2215	4	-3.8511551262399095
2215	5	-4.750126730448084
2215	6	-1.989746400416081
2215	7	-1.1775260337409605
2215	8	-1.608061530143555
2215	9	-1.1604372923020208
2215	10	-2.5197512548939542
2215	11	-1.4357757330919938
2215	12	-0.9671231181125322
2215	13	-1.0940265755033494
2215	14	-0.22884940624041405
2215	15	-0.7546739349024989
2215	16	-1.545273863405832
2215	17	-6.144102955377786
2215	18	-2.2431974386994575
2215	19	-1.3355793655694086
2215	20	-1.1929388442886903
2215	21	-1.1332410510119146
2215	22	-0.7642179390286046
2215	23	-5.973083125901425
2215	24	-2.8889530261764023
2215	25	-2.3860095703311117
2216	1	-0.5936290393761396
2216	2	-0.047674141338173825
2216	3	-0.38406760678695306
2216	4	-3.014144736799853
2216	5	-0.7793570908804881
2216	6	-0.5090927829338199
2216	7	-0.4898799202636397
2216	8	-0.32875813958696654
2216	9	-0.28486912645252715
2216	10	0.09064346547518909
2216	11	-0.2069968393514685
2216	12	-1.0322246832141033
2216	13	-0.44495697502709675
2216	14	-6.269552522391098
2216	15	-2.3728941630971505
2216	16	-0.6243669783045789
2216	17	0.18210238233650244
2216	18	0.9530807077065688
2216	19	0.43450775106501993
2216	20	-0.17110986338042977
2216	21	-0.29438553312826343
2216	22	0.29629486445095665
2216	23	1.6413936164525538
2216	24	0.781408581525362
2216	25	1.1324076475745954
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68457.0
3	1.0
4	598.0
5	3525.0
6	4506.0
7	3925.0
8	3706.0
9	3461.0
10	3290.0
11	3184.0
12	3190.0
13	3237.0
14	3161.0
15	3485.0
16	3361.0
17	3633.0
18	3988.0
19	4865.0
20	6005.0
21	7461.0
22	9296.0
23	11294.0
24	13363.0
25	15904.0
26	18082.0
27	21062.0
28	23766.0
29	28585.0
30	34068.0
31	38690.0
32	50538.0
33	66947.0
34	88530.0
35	118451.0
36	172420.0
37	246481.0
38	627789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2500870278774	18.114525780882694	12.291125829815897	25.344261361424014
2	13.99468818655944	19.533810529055224	41.954961119895714	24.51654016448962
3	19.463761625884228	24.124628201129468	28.777711757643765	27.63389841534254
4	10.828786079158522	16.191341873168636	38.78610819899694	34.1937638486759
5	12.401005755388162	37.9683113868865	36.10636287231565	13.524319985409694
6	32.875709997543574	35.873016057370066	18.001025543941886	13.250248401144473
7	28.88179243990086	30.952132070606766	21.469952171559846	18.69612331793253
8	25.69172462495528	36.75054938417867	20.032602989207447	17.525123001658606
9	26.773923583221972	15.167665884367192	19.564281843857206	38.49412868855363
10	14.31618185588159	29.171470439445745	34.763439480013496	21.748908224659168
11	34.67459627964023	22.57146407864417	23.503702705716197	19.250236935999403
12	23.614736576808028	24.817697550698288	31.279308200169453	20.28825767232423
13	29.181496315162626	21.356087516266882	25.2345305643193	24.227885604251195
14	20.775765797522944	22.01301653984435	27.89862499949186	29.312592663140848
15	24.34906986751717	29.780093486546427	22.708148103410128	23.162688542526276
16	22.028869528454305	27.93802854199357	28.270673897212124	21.762428032340004
17	22.154203004938154	28.19422045079299	28.12993096575351	21.521645578515347
18	21.729886869721607	26.91206779105887	30.30696336869344	21.05108197052608
19	23.539005069726894	27.00516729982594	27.72250258306912	21.733325047378045
20	23.54690565209362	27.122279095860147	28.00796308362748	21.322852168418756
21	22.830578452399827	26.699607922395234	27.54416317769287	22.92565044751207
22	22.905130144170958	27.639620237136885	26.871254441815267	22.58399517687689
23	21.116822022824287	27.137323227923453	29.305746725905273	22.44010802334698
24	22.75738371258918	27.606131520665215	27.68105916321506	21.955425603530543
25	22.619246745281703	26.778130894966225	27.54395379740366	23.058668562348412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	161.0
2	292.0
3	423.0
4	423.0
5	423.0
6	888.0
7	1353.0
8	1353.0
9	1353.0
10	2100.0
11	2847.0
12	2847.0
13	2847.0
14	5051.5
15	7256.0
16	7256.0
17	7256.0
18	12917.5
19	18579.0
20	18579.0
21	18579.0
22	29111.0
23	39643.0
24	39643.0
25	39643.0
26	56614.5
27	73586.0
28	73586.0
29	73586.0
30	94920.5
31	116255.0
32	116255.0
33	116255.0
34	134932.0
35	153609.0
36	153609.0
37	153609.0
38	169658.0
39	185707.0
40	185707.0
41	185707.0
42	199364.5
43	213022.0
44	213022.0
45	213022.0
46	232162.5
47	251303.0
48	251303.0
49	251303.0
50	237930.5
51	224558.0
52	224558.0
53	224558.0
54	195895.5
55	167233.0
56	167233.0
57	167233.0
58	142712.5
59	118192.0
60	118192.0
61	118192.0
62	96504.5
63	74817.0
64	74817.0
65	74817.0
66	58030.5
67	41244.0
68	41244.0
69	41244.0
70	30347.0
71	19450.0
72	19450.0
73	19450.0
74	13733.0
75	8016.0
76	8016.0
77	8016.0
78	5649.5
79	3283.0
80	3283.0
81	3283.0
82	2245.5
83	1208.0
84	1208.0
85	1208.0
86	804.0
87	400.0
88	400.0
89	400.0
90	261.5
91	123.0
92	123.0
93	123.0
94	75.5
95	28.0
96	28.0
97	28.0
98	18.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09243426686910855
2	0.049875022136032815
3	0.03530152905553894
4	0.03309518348956776
5	0.03564989940806071
6	0.01695402382272594
7	0.018405566958233296
8	0.021889270483450955
9	0.026592270242494793
10	0.03297906003872717
11	0.018637813859914476
12	0.01765076452776947
13	0.014399307904232989
14	0.019857110093740655
15	0.02008935699542183
16	0.020379665622523305
17	0.023573060520639493
18	0.01788301142945065
19	0.029959850316871865
20	0.020844159425885658
21	0.026243899889973034
22	0.034778973526756296
23	0.02641808506623391
24	0.03739175117066954
25	0.028856677533886276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1722305.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.42457655800642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94619414451282	67.21250647382615
2	8.554842715944897	13.076004641728685
3	1.856877362448531	4.257331984358304
4	0.6431316472521552	1.9660425524919645
5	0.3139750133260793	1.1997703721620017
6	0.162791869164273	0.7464779800779567
7	0.10754285887964461	0.5753242216199995
8	0.06753373356925908	0.4128989593129479
9	0.05303869678500574	0.36481139486843034
>10	0.2619019395893182	3.4928270929663654
>50	0.017314479212416682	0.9230297021390601
>100	0.011315323187758165	1.7829275340583655
>500	0.0019240305042815444	0.9671914613398037
>1k	0.0014622631832539735	2.070074402934467
>5k	1.5392244034252353E-4	0.9527812261155119
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	9013	0.5233103312131127	No Hit
CCCATGTACTCTGCGTTGATACCAC	7186	0.41723155887023494	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3726	0.2163379889160166	No Hit
GTATCAACGCAGAGTACATGGGAAG	2612	0.15165722679780874	No Hit
GCGTTGATACCACTGCTTCCCATGT	2341	0.135922499208909	No Hit
GTCCTACAGTGGACATTTCTAAATT	2279	0.13232267223285074	No Hit
CATGTACTCTGCGTTGATACCACTG	2269	0.1317420549786478	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2180	0.1265745614162416	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1944	0.11287199421705216	No Hit
TATCAACGCAGAGTACATGGGAAGC	1836	0.10660132787166036	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1811	0.10514978473615301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	5.8061725420294315E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	615	0.0	14.05893	4
TGCGTTT	215	0.0	13.254254	12
GCGTTTA	210	0.0	13.117503	13
CGTTTAT	215	0.0	12.370638	14
AGGACCT	1065	0.0	11.775656	5
GTAGGAC	1345	0.0	11.513997	3
TGTAGGA	1485	0.0	11.452491	2
TGGCGAG	1035	0.0	11.197725	18
CTGTAGG	1425	0.0	11.14335	1
GTCCTAC	1375	0.0	11.064491	1
GGACCTG	1075	0.0	11.045853	6
GTGTAGG	260	0.0	10.971401	1
GGCGAGA	745	0.0	10.966107	19
ACTGTCG	70	0.0014930087	10.8558655	8
ATGGCGA	1165	0.0	10.762995	17
TCCTACA	1545	0.0	10.700255	2
GTCTAGG	170	1.364242E-10	10.627199	1
TAGGACT	340	0.0	10.619173	4
TCTTAGG	215	0.0	10.605868	2
ACCGTGC	90	9.526886E-5	10.554313	8
>>END_MODULE