FastQCFastQC Report
Thu 26 May 2016
SRR1513114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC97100.5637793538310578No Hit
CCCATGTACTCTGCGTTGATACCAC94630.5494381076522451No Hit
GAGTACATGGGAAGCAGTGGTATCA36560.21227366813659604No Hit
GTATCAACGCAGAGTACTTTTTTTT35020.2033321624218707No Hit
CATGTACTCTGCGTTGATACCACTG27210.15798595486862083No Hit
GTATCAACGCAGAGTACATGGGAAG25580.14852189362511287No Hit
TATCAACGCAGAGTACTTTTTTTTT25300.14689616531334462No Hit
ACGCAGAGTACATGGGAAGCAGTGG24210.14056743724253254No Hit
GCGTTGATACCACTGCTTCCCATGT23970.13917395583244546No Hit
TATCAACGCAGAGTACATGGGAAGC23190.1346451412496625No Hit
GCTTCCCATGTACTCTGCGTTGATA21600.12541332690783571No Hit
GGTATCAACGCAGAGTACTTTTTTT21520.12494883310447337No Hit
GTCCTACAGTGGACATTTCTAAATT21250.12338116651812543No Hit
ACTCTGCGTTGATACCACTGCTTCC19220.11159463625780568No Hit
CTGTAGGACGTGGAATATGGCAAGA18510.10747225375296476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG350.00213347416.3275181
TCCAACG1102.7812348E-912.94365118
CAGATCG604.1367454E-412.6505019
TAGGACC5800.012.3066884
CCTATAC2150.011.5091383
GTCCTAG2200.011.2560921
GTAGGAC11400.010.93639853
TGTAGGA12250.010.8050582
GTCCTAC13400.010.8037811
ATAGGAC2350.010.5296373
TCCTACA15300.010.5182532
AGGACGT10800.010.3102695
CTGTAGG13350.010.1308071
CCAACGT750.002677949610.11951919
GATATAC2850.010.0256691
CTAAGAC1909.640644E-1110.0180763
TAGGACA4750.010.0180754
GACGTGA5500.010.0053977
CACCGCT951.6653762E-49.9872387
GGCGAGG5700.09.98636719
Produced by FastQC (version 0.11.5)