##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513114_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1722305 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.110135545097993 31.0 30.0 34.0 26.0 34.0 2 30.27931754247941 31.0 31.0 34.0 26.0 34.0 3 30.30948583439054 31.0 31.0 34.0 26.0 34.0 4 33.466211269200286 37.0 35.0 37.0 28.0 37.0 5 33.40541657836446 37.0 35.0 37.0 28.0 37.0 6 33.37717535512003 37.0 35.0 37.0 28.0 37.0 7 33.270793500570456 37.0 35.0 37.0 27.0 37.0 8 33.26407053338404 37.0 35.0 37.0 27.0 37.0 9 34.774341362302266 39.0 35.0 39.0 27.0 39.0 10 34.66329598996693 39.0 35.0 39.0 27.0 39.0 11 34.67151346596567 39.0 35.0 39.0 27.0 39.0 12 34.58190041833473 39.0 35.0 39.0 27.0 39.0 13 34.54948165394631 39.0 35.0 39.0 27.0 39.0 14 35.678288108087706 40.0 36.0 41.0 27.0 41.0 15 35.66296097381126 40.0 36.0 41.0 27.0 41.0 16 35.608845123250525 40.0 36.0 41.0 26.0 41.0 17 35.525815114047745 40.0 36.0 41.0 25.0 41.0 18 35.454497896713995 40.0 36.0 41.0 25.0 41.0 19 35.51585056073111 40.0 36.0 41.0 25.0 41.0 20 35.426084230145065 40.0 36.0 41.0 25.0 41.0 21 35.433176469905156 40.0 36.0 41.0 25.0 41.0 22 35.403956906587396 40.0 36.0 41.0 25.0 41.0 23 35.32578201886425 40.0 36.0 41.0 25.0 41.0 24 35.193922679200256 40.0 36.0 41.0 23.0 41.0 25 35.17015453128221 40.0 36.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8641946580956272 1101 2 0.7641838725584016 1101 3 0.8623853995955955 1101 4 1.0837741563973466 1101 5 0.9715211127785821 1101 6 0.9344931050686469 1101 7 0.8288413511783048 1101 8 0.8349578876001402 1101 9 0.9039661306721101 1101 10 0.8472098912999542 1101 11 0.8080683250576186 1101 12 0.8286490115749103 1101 13 0.824539455610406 1101 14 0.9488224787278625 1101 15 0.941334785291474 1101 16 0.9683639974863922 1101 17 0.99892877993247 1101 18 0.9835967309333711 1101 19 0.9997283331951436 1101 20 1.1671239214573887 1101 21 1.057403918875437 1101 22 1.2778913135530345 1101 23 1.0965776008639736 1101 24 1.1549587026042971 1101 25 1.0964353439499632 1102 1 0.813973791743777 1102 2 0.7802180513033079 1102 3 0.76493095216928 1102 4 1.075878282120577 1102 5 0.9061352599376207 1102 6 0.73710679890101 1102 7 0.782064416117116 1102 8 0.8152033241629795 1102 9 0.7799112231381855 1102 10 0.7900281369399451 1102 11 0.841644965835826 1102 12 0.8260340848457304 1102 13 0.7563198368552975 1102 14 0.8234474232178712 1102 15 0.8681879655369613 1102 16 0.9041550869143293 1102 17 0.9391620914293028 1102 18 1.0232693351836915 1102 19 1.060599412731456 1102 20 1.0957332683800942 1102 21 1.1134086124187803 1102 22 1.1067264702696349 1102 23 1.0639808759595297 1102 24 1.1871598335974554 1102 25 1.258794577363176 1103 1 0.46352378806898287 1103 2 0.6086818726703171 1103 3 0.8232023716937711 1103 4 -0.028323121908734095 1103 5 0.3926466554362449 1103 6 0.5022702520607751 1103 7 0.6044786824863237 1103 8 0.6268054800103755 1103 9 0.8862153602979106 1103 10 0.8379785532951942 1103 11 0.9638525045391475 1103 12 0.9121937803239533 1103 13 0.8571223506486803 1103 14 0.9013586754347784 1103 15 1.0618336022894042 1103 16 0.9497136003066586 1103 17 0.9925273684605784 1103 18 0.9205382006681759 1103 19 0.9521401795727513 1103 20 1.107677050852999 1103 21 1.0374789308202423 1103 22 1.08205604521698 1103 23 1.0887918354374762 1103 24 1.020907373657984 1103 25 1.1111706188383224 1104 1 0.6399804216823455 1104 2 0.5527065481759337 1104 3 0.6608948529832865 1104 4 0.43191174488036665 1104 5 0.402419585800736 1104 6 0.6093730598995677 1104 7 0.5441824840012686 1104 8 0.5615812750878248 1104 9 0.5641966513301071 1104 10 0.42404386595031696 1104 11 0.47217149096986333 1104 12 0.5494679085001906 1104 13 0.6031048095035842 1104 14 0.5463856154055691 1104 15 0.5215319334278163 1104 16 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14 -3.9487743398587725 2216 15 -3.824866150773797 2216 16 -3.7845685802037146 2216 17 -3.749814812135291 2216 18 -3.7794043754825424 2216 19 -3.8165713745901932 2216 20 -3.5931917164151237 2216 21 -3.707730167511329 2216 22 -4.060987048318349 2216 23 -4.0354901883328615 2216 24 -4.083223581216561 2216 25 -4.265040376047676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58412.0 3 0.0 4 976.0 5 7338.0 6 8328.0 7 6283.0 8 5034.0 9 4343.0 10 3874.0 11 3489.0 12 3087.0 13 2979.0 14 2839.0 15 2910.0 16 2880.0 17 3043.0 18 3404.0 19 4125.0 20 5171.0 21 6566.0 22 8123.0 23 9792.0 24 11035.0 25 12512.0 26 13488.0 27 14951.0 28 16815.0 29 19933.0 30 25147.0 31 32021.0 32 40613.0 33 52781.0 34 70775.0 35 96227.0 36 142467.0 37 242101.0 38 778443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.71196847773862 19.590487480557435 13.856602561939917 27.840941479764027 2 13.32351871497442 20.5160575909721 39.13697202368987 27.023451670363606 3 17.32254159396855 24.35015865366471 29.217763404275082 29.10953634809166 4 10.394964887171552 17.33316688972046 36.83772618670909 35.434142036398896 5 12.689332126965914 38.02914562008486 35.665521082199845 13.616001170749383 6 33.01697198605508 36.587632656462226 17.291066618264626 13.104328739218065 7 28.288913687970524 31.104067500984158 21.552401865892193 19.05461694515313 8 25.6129362746066 37.41835550594322 19.48781329241477 17.480894927035408 9 25.968963208060575 15.01321610465552 19.442919844368824 39.574900842915085 10 14.218227973341913 29.816217584630373 34.13880879359258 21.826745648435132 11 34.51917270531401 22.548947881828315 23.370784559643255 19.56109485321442 12 23.178791795722265 25.21133761408863 31.190110363585475 20.41976022660363 13 29.065758812930532 21.26577309734246 25.375677130482295 24.292790959244716 14 20.727538126918247 22.119999489047622 27.24891625838862 29.903546125645512 15 24.102421180978922 30.06386808337688 22.57998025895605 23.25373047668815 16 22.02465947040149 28.20630893614007 27.669753794356534 22.09927779910191 17 22.177761490566354 28.608448145590636 27.363347618198596 21.850442745644415 18 22.164155127647227 27.423426480183178 28.976015998243078 21.436402393926524 19 23.303596320044605 27.18590279713781 27.633526159278876 21.876974723538705 20 23.469246155588007 27.19431227337783 27.86794441170408 21.468497159330084 21 22.601165298829184 26.78451261536139 27.501981356379968 23.112340729429455 22 22.81140680657607 27.65584492874375 26.68290459587588 22.849843668804308 23 21.84816278185339 27.505232813003506 27.44682271723069 23.19978168791242 24 22.67561359667051 27.689362621232956 27.680479172085764 21.954544610010775 25 22.538458635375267 26.799144170167306 27.203718272895916 23.458678921561514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 130.0 1 130.0 2 270.0 3 410.0 4 410.0 5 410.0 6 1001.0 7 1592.0 8 1592.0 9 1592.0 10 2276.5 11 2961.0 12 2961.0 13 2961.0 14 5013.0 15 7065.0 16 7065.0 17 7065.0 18 12669.0 19 18273.0 20 18273.0 21 18273.0 22 28846.5 23 39420.0 24 39420.0 25 39420.0 26 56259.0 27 73098.0 28 73098.0 29 73098.0 30 94482.5 31 115867.0 32 115867.0 33 115867.0 34 134191.0 35 152515.0 36 152515.0 37 152515.0 38 167385.5 39 182256.0 40 182256.0 41 182256.0 42 194830.5 43 207405.0 44 207405.0 45 207405.0 46 229907.0 47 252409.0 48 252409.0 49 252409.0 50 240807.5 51 229206.0 52 229206.0 53 229206.0 54 199387.5 55 169569.0 56 169569.0 57 169569.0 58 144458.5 59 119348.0 60 119348.0 61 119348.0 62 97520.0 63 75692.0 64 75692.0 65 75692.0 66 58825.0 67 41958.0 68 41958.0 69 41958.0 70 30841.5 71 19725.0 72 19725.0 73 19725.0 74 14001.5 75 8278.0 76 8278.0 77 8278.0 78 5823.5 79 3369.0 80 3369.0 81 3369.0 82 2298.5 83 1228.0 84 1228.0 85 1228.0 86 822.0 87 416.0 88 416.0 89 416.0 90 257.5 91 99.0 92 99.0 93 99.0 94 57.0 95 15.0 96 15.0 97 15.0 98 8.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18225575609430386 2 0.061719614121772855 3 0.0 4 0.0 5 0.019218431114117418 6 0.35557000647388237 7 0.0011031727829855919 8 0.029669541689770394 9 0.03146945517779952 10 0.00296114799643501 11 0.0037740121523191306 12 0.010393048850232681 13 0.008593135362203559 14 0.0017999134880291236 15 2.903086271014716E-4 16 0.012076838887421218 17 0.025314912283248323 18 0.06479688556904846 19 0.0316436403540604 20 0.0 21 0.0 22 0.0 23 0.0 24 5.8061725420294315E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1722305.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.98214370809183 #Duplication Level Percentage of deduplicated Percentage of total 1 85.81436585764429 62.629163812413644 2 9.8682953925459 14.404187049853705 3 2.337860002692135 5.118661040576321 4 0.774345462233642 2.260535672177778 5 0.3669065286072717 1.3388812499126501 6 0.20477721743085653 0.8967048186409162 7 0.12510425961461613 0.6391263937581839 8 0.08820847047343042 0.51501146146903 9 0.06469982421900872 0.42497386821359767 >10 0.3182991024580135 4.006627063955333 >50 0.019539347641078623 0.983822552519197 >100 0.013378825933590962 1.8951006121507235 >500 0.0022292799655377784 1.12121910147086 >1k 0.0018311942574060322 2.651913275913587 >5k 1.5923428325269846E-4 1.1140720269745437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9710 0.5637793538310578 No Hit CCCATGTACTCTGCGTTGATACCAC 9463 0.5494381076522451 No Hit GAGTACATGGGAAGCAGTGGTATCA 3656 0.21227366813659604 No Hit GTATCAACGCAGAGTACTTTTTTTT 3502 0.2033321624218707 No Hit CATGTACTCTGCGTTGATACCACTG 2721 0.15798595486862083 No Hit GTATCAACGCAGAGTACATGGGAAG 2558 0.14852189362511287 No Hit TATCAACGCAGAGTACTTTTTTTTT 2530 0.14689616531334462 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2421 0.14056743724253254 No Hit GCGTTGATACCACTGCTTCCCATGT 2397 0.13917395583244546 No Hit TATCAACGCAGAGTACATGGGAAGC 2319 0.1346451412496625 No Hit GCTTCCCATGTACTCTGCGTTGATA 2160 0.12541332690783571 No Hit GGTATCAACGCAGAGTACTTTTTTT 2152 0.12494883310447337 No Hit GTCCTACAGTGGACATTTCTAAATT 2125 0.12338116651812543 No Hit ACTCTGCGTTGATACCACTGCTTCC 1922 0.11159463625780568 No Hit CTGTAGGACGTGGAATATGGCAAGA 1851 0.10747225375296476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.8061725420294315E-5 0.0 0.0 0.0 0.0 10 5.8061725420294315E-5 0.0 0.0 0.0 0.0 11 5.8061725420294315E-5 0.0 0.0 0.0 0.0 12 5.8061725420294315E-5 0.0 0.0 0.0 0.0 13 5.8061725420294315E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGG 35 0.002133474 16.327518 1 TCCAACG 110 2.7812348E-9 12.943651 18 CAGATCG 60 4.1367454E-4 12.650501 9 TAGGACC 580 0.0 12.306688 4 CCTATAC 215 0.0 11.509138 3 GTCCTAG 220 0.0 11.256092 1 GTAGGAC 1140 0.0 10.9363985 3 TGTAGGA 1225 0.0 10.805058 2 GTCCTAC 1340 0.0 10.803781 1 ATAGGAC 235 0.0 10.529637 3 TCCTACA 1530 0.0 10.518253 2 AGGACGT 1080 0.0 10.310269 5 CTGTAGG 1335 0.0 10.130807 1 CCAACGT 75 0.0026779496 10.119519 19 GATATAC 285 0.0 10.025669 1 CTAAGAC 190 9.640644E-11 10.018076 3 TAGGACA 475 0.0 10.018075 4 GACGTGA 550 0.0 10.005397 7 CACCGCT 95 1.6653762E-4 9.987238 7 GGCGAGG 570 0.0 9.986367 19 >>END_MODULE