FastQCFastQC Report
Thu 26 May 2016
SRR1513113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265141
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC99340.4385598953884107No Hit
CCCATGTACTCTGCGTTGATACCAC82620.3647455059089037No Hit
GAGTACATGGGAAGCAGTGGTATCA41190.18184298460890513No Hit
GTCCTACAGTGGACATTTCTAAATT31120.13738659094511113No Hit
GCGTTGATACCACTGCTTCCCATGT28310.12498118218689255No Hit
CATGTACTCTGCGTTGATACCACTG27860.12299455089109243No Hit
GTATCAACGCAGAGTACATGGGAAG27330.12065474069826118No Hit
GTCCTACAGTGTGCATTTCTCATTT26490.11694636227943425No Hit
CTGTAGGACGTGGAATATGGCAAGA23730.10476169033186013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG402.758976E-416.6272435
TGATACG500.001500889213.29885717
TAGGACC8500.012.7437394
GTCCTAC19300.011.7274861
GGCGAGG6550.011.45728219
AGGACCG855.317806E-511.17797855
TGGCGAG15350.011.13935318
TGTAGGA18400.011.1017952
GTAGGAC18150.011.0976713
CTACGCT600.00586827111.0855644
TCCTACA20800.011.0541312
GACGTGA8100.011.02349957
TTAGGAC10300.010.9769653
GGACGTG14750.010.884296
AGATGCG700.001490180710.8586075
AGGACGT15000.010.7047775
ACCGTAT803.7758774E-410.6863458
AGGACCT13350.010.6044255
TAGGACG15850.010.371174
CGTTTAT1659.331416E-1010.3618314
Produced by FastQC (version 0.11.5)