FastQCFastQC Report
Thu 26 May 2016
SRR1513113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265141
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC115000.5076946644822552No Hit
CCCATGTACTCTGCGTTGATACCAC111030.49016816171708516No Hit
GAGTACATGGGAAGCAGTGGTATCA42370.18705237334011438No Hit
GTCCTACAGTGGACATTTCTAAATT32520.143567221643156No Hit
GTATCAACGCAGAGTACTTTTTTTT32020.1413598535367114No Hit
CATGTACTCTGCGTTGATACCACTG31920.14091837991542247No Hit
GTATCAACGCAGAGTACATGGGAAG29940.13217720221390192No Hit
GCGTTGATACCACTGCTTCCCATGT29180.12882200269210614No Hit
GTCCTACAGTGTGCATTTCTCATTT28200.12449556120347476No Hit
CTGTAGGACGTGGAATATGGCAAGA27930.12330358242599467No Hit
ACGCAGAGTACATGGGAAGCAGTGG26680.1177851621598832No Hit
TATCAACGCAGAGTACATGGGAAGC25350.1119135629967406No Hit
GCTTCCCATGTACTCTGCGTTGATA24800.10948545807965156No Hit
ACTCTGCGTTGATACCACTGCTTCC23180.10233358541477108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA2650.012.9332034
TAGGACC9300.012.8984364
TACACGG853.852625E-612.32035355
TGGACCG701.06981955E-412.2403525
GTTACCC550.003020571312.1167123
GTAGGAC18350.011.6214793
ACACCGT1354.5765773E-911.2832876
GGGCCGT855.210717E-511.2003216
GGACCGA600.005784534411.1069866
TGTAGGA20200.011.0804762
GTCCTAC19900.011.059751
GGACGTG14050.010.9093536
AGGACGT14000.010.8803135
GGACCGT803.6985168E-410.7103086
TGGCGAA1252.2727181E-710.62926418
CTGTAGG20100.010.4756891
ACCGTCC1002.434282E-510.4364598
GACGTGG7500.010.3734367
TAGGACG15250.010.3630514
GGCGAAA1106.150389E-610.34906419
Produced by FastQC (version 0.11.5)