FastQCFastQC Report
Thu 26 May 2016
SRR1513112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956519
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC121170.6193142003732138No Hit
CCCATGTACTCTGCGTTGATACCAC98970.5058473748529915No Hit
GAGTACATGGGAAGCAGTGGTATCA51050.2609225875138448No Hit
GCGTTGATACCACTGCTTCCCATGT34210.17485135590300938No Hit
GTATCAACGCAGAGTACATGGGAAG33390.1706602389243345No Hit
CATGTACTCTGCGTTGATACCACTG32510.16616245484965902No Hit
GTATCAACGCAGAGTACTTTTTTTT29300.14975576521362688No Hit
TATCAACGCAGAGTACATGGGAAGC25840.13207129601092552No Hit
ACGCAGAGTACATGGGAAGCAGTGG24850.1270112889269156No Hit
GCTTCCCATGTACTCTGCGTTGATA22060.11275126896288766No Hit
ACTCTGCGTTGATACCACTGCTTCC21850.11167793412688556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC250.005988350619.0242461
TGCGCGA350.002172382816.28356610
TAGGACC3750.014.6951524
GCGTTTA1550.013.48209313
GTAGGAC9000.012.9836133
TGTAGGA10550.012.6088412
GCCGGTT1001.4465877E-712.3477411
ATAGGAC2300.011.97851853
GACGTGG3900.011.9343247
TAGGACA4300.011.931714
CTGTAGG10100.011.772431
TATCGAC658.030488E-411.69136319
GTCCTAC10000.011.6047891
AGGACCG600.005874519711.0838555
ACAGCGC1053.4725854E-610.8557118
TGCGTTT2100.010.85543312
TGCACCG803.7699708E-410.6880045
ACGTGGA4550.010.6469488
TAGGACG7850.010.5299664
TCCTACA11500.010.493142
Produced by FastQC (version 0.11.5)