##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513112_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1956519
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.451690987922937	31.0	30.0	34.0	25.0	34.0
2	29.118402632430353	31.0	30.0	34.0	18.0	34.0
3	29.501267301774224	31.0	30.0	34.0	24.0	34.0
4	32.36770918145952	35.0	33.0	37.0	19.0	37.0
5	32.60341555589289	35.0	33.0	37.0	24.0	37.0
6	33.05588445601602	37.0	35.0	37.0	27.0	37.0
7	33.033117490808934	37.0	35.0	37.0	27.0	37.0
8	33.01684931247792	37.0	35.0	37.0	26.0	37.0
9	34.40396438777236	39.0	35.0	39.0	27.0	39.0
10	33.91065100824474	39.0	34.0	39.0	22.0	39.0
11	34.297821283616464	39.0	35.0	39.0	27.0	39.0
12	34.13930046168731	39.0	35.0	39.0	25.0	39.0
13	34.22768140764286	39.0	35.0	39.0	26.0	39.0
14	34.54458045130152	40.0	34.0	41.0	17.0	41.0
15	35.093809464666585	40.0	34.0	41.0	25.0	41.0
16	34.68348224576403	40.0	34.0	41.0	18.0	41.0
17	34.227815318941445	39.0	33.0	41.0	17.0	41.0
18	33.63680751375274	39.0	32.0	41.0	11.0	41.0
19	34.53833926478608	39.0	34.0	41.0	18.0	41.0
20	34.80392472549462	40.0	34.0	41.0	19.0	41.0
21	34.82288646315216	40.0	35.0	41.0	19.0	41.0
22	34.419909543428915	40.0	34.0	41.0	16.0	41.0
23	32.7434193074537	39.0	31.0	41.0	8.0	41.0
24	33.854490040730504	39.0	33.0	41.0	2.0	41.0
25	33.59218131794273	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5611119916976541
1101	2	0.8023057791722117
1101	3	0.6069993629270556
1101	4	0.4822187519418364
1101	5	0.8157629322535342
1101	6	0.483419933820457
1101	7	0.45419113233957376
1101	8	0.5612142088156133
1101	9	0.6768197631815553
1101	10	0.8788616901448094
1101	11	0.643449603488186
1101	12	0.6753639903340485
1101	13	0.6065912001984444
1101	14	-0.29494274696452294
1101	15	0.35813745787578455
1101	16	0.980691274677028
1101	17	1.5305041656188507
1101	18	-4.525425751960363
1101	19	-0.9382384561383645
1101	20	0.011825800759623917
1101	21	0.41732642935828324
1101	22	0.8871113360751437
1101	23	2.709719456458025
1101	24	1.6477313453915983
1101	25	1.949989055879108
1102	1	0.9632344150171406
1102	2	1.322001320067912
1102	3	1.0085278488785612
1102	4	1.666067301008212
1102	5	1.3400321691867418
1102	6	0.9904722092505693
1102	7	0.8322527298694524
1102	8	0.7787672418855749
1102	9	1.0111473483567153
1102	10	1.2912093281862411
1102	11	0.92682135187885
1102	12	1.0530627598829057
1102	13	0.9541793742760873
1102	14	1.813956890345061
1102	15	1.412360466053343
1102	16	1.566395026834222
1102	17	1.2287970513703428
1102	18	1.829744323225846
1102	19	1.4860728757188966
1102	20	1.3752731686919546
1102	21	1.3285202400287943
1102	22	1.6635360463458255
1102	23	3.2605937859140823
1102	24	1.9905411953286958
1102	25	2.299385867257392
1103	1	0.9110164567981869
1103	2	1.2798426597149053
1103	3	0.9657749341443242
1103	4	1.4636551431480882
1103	5	1.3670186708909853
1103	6	1.0046602065645942
1103	7	0.8863462309949881
1103	8	0.817027296602447
1103	9	0.9934580747410777
1103	10	1.2879477715969614
1103	11	1.0321950397700448
1103	12	1.1585002338610835
1103	13	0.977293016728396
1103	14	1.9300523236091607
1103	15	1.267368775640854
1103	16	1.5740056348084082
1103	17	2.0725772700290435
1103	18	2.071251785618152
1103	19	1.5719639731527266
1103	20	1.453997377367564
1103	21	1.4705536477019194
1103	22	1.7048134149261926
1103	23	3.177318729869306
1103	24	2.080242409699821
1103	25	2.2833400711664353
1104	1	0.7236649543523441
1104	2	1.1789405569260119
1104	3	0.9322573685512872
1104	4	1.5397684777519913
1104	5	1.3090871544689051
1104	6	0.8150725056561399
1104	7	0.8084159851402148
1104	8	0.7764390239386998
1104	9	0.9692537675178983
1104	10	1.3317315210410925
1104	11	0.8405871368413855
1104	12	0.968588538289012
1104	13	0.830402730215468
1104	14	1.7044821565295578
1104	15	1.1114193442197262
1104	16	1.4133042816542272
1104	17	1.9623619088702924
1104	18	2.5408089929112094
1104	19	1.7564630519914246
1104	20	1.2466789096717363
1104	21	1.2892881626628494
1104	22	1.6690024753416637
1104	23	3.2832776905452477
1104	24	2.142817849633751
1104	25	2.1790137894816652
1105	1	0.7895983823547397
1105	2	1.1693253955867107
1105	3	0.8790419696462308
1105	4	1.048576272766681
1105	5	0.9873965526058939
1105	6	0.7625115289572335
1105	7	0.5085867913455075
1105	8	0.5805173849136125
1105	9	0.8734763648439454
1105	10	1.2455183727589727
1105	11	0.8551838109208205
1105	12	0.7911394229088629
1105	13	0.7868560789101906
1105	14	1.6691150013348164
1105	15	1.0529960306239943
1105	16	1.3284028803735168
1105	17	1.6928189996722054
1105	18	1.762547152580403
1105	19	1.351607020411258
1105	20	1.1136228852364027
1105	21	0.9892707471281881
1105	22	1.3609732440365718
1105	23	2.7781537276626693
1105	24	1.8138495637689118
1105	25	1.7451392349174526
1106	1	-0.41896048705358524
1106	2	0.011007157895051023
1106	3	-0.1743103410652047
1106	4	-2.540723433227729
1106	5	-0.3693332282197659
1106	6	-0.35964006722716135
1106	7	-0.2268944361458125
1106	8	-0.20227699807000477
1106	9	-0.3598851829625147
1106	10	-0.22358588061777596
1106	11	-0.26540161244004423
1106	12	-0.17984909002666427
1106	13	-0.339076616093152
1106	14	0.43610119241688494
1106	15	-0.1261227433223624
1106	16	-1.1494451640220475
1106	17	-6.1519088474736385
1106	18	-7.37009681311655
1106	19	-2.991409987345026
1106	20	-1.58358100656379
1106	21	-1.0870422142772895
1106	22	-1.5319645116009326
1106	23	-5.477104587519953
1106	24	-2.2484936392387134
1106	25	-0.8206166613682981
1107	1	0.5005041426959593
1107	2	0.7285397941358696
1107	3	0.47540566568188325
1107	4	0.7910810131353188
1107	5	0.9210623637892468
1107	6	0.3859941110910228
1107	7	0.3597315001725434
1107	8	0.40484401783042046
1107	9	0.3490577066138769
1107	10	0.7603897850975869
1107	11	0.5160884627851914
1107	12	0.5878430301098732
1107	13	0.5361921882173135
1107	14	1.3584227423285498
1107	15	0.8185675221643649
1107	16	-5.631742051887681
1107	17	-0.9776666957600852
1107	18	0.7086138854948629
1107	19	0.5423514343399845
1107	20	0.4815765587286762
1107	21	0.4932123603923557
1107	22	0.5522032142946145
1107	23	2.412716622639053
1107	24	1.4817827195720739
1107	25	1.015818413896909
1108	1	0.4523179197258642
1108	2	0.7225840744725325
1108	3	0.48669229296311656
1108	4	1.0788890355792446
1108	5	0.7232613388509392
1108	6	0.30861776802357355
1108	7	0.2807283532955367
1108	8	0.33392557980862847
1108	9	0.433121744549851
1108	10	0.8658991069407733
1108	11	0.46890206885147023
1108	12	0.5612873286783326
1108	13	0.3709006379189148
1108	14	0.8848021273807092
1108	15	0.5986563908101203
1108	16	0.8325171960172568
1108	17	0.9116079156220707
1108	18	0.923660825878514
1108	19	0.7890337829455305
1108	20	0.5076408884166241
1108	21	0.6111577993130837
1108	22	0.9506573613845433
1108	23	2.6390744128546757
1108	24	1.3889823429114045
1108	25	1.4544537716191073
1109	1	0.53682296324871
1109	2	0.833111269669569
1109	3	0.6855978430017693
1109	4	0.6395168701663891
1109	5	0.4888714526872988
1109	6	0.4132048031937927
1109	7	0.43249322933638723
1109	8	0.24820558282121397
1109	9	0.47590201410667987
1109	10	0.9392991030387989
1109	11	0.6192842500280946
1109	12	0.601920203137027
1109	13	0.4734903624012148
1109	14	-0.6262660256556885
1109	15	0.1510218442781195
1109	16	0.06518683363588451
1109	17	1.01792947752012
1109	18	1.904969594633755
1109	19	1.0429239610489631
1109	20	0.7823367767468454
1109	21	0.8972398299802222
1109	22	0.717336159056913
1109	23	-4.285694822827658
1109	24	-1.1471348654924043
1109	25	0.09484204227720738
1110	1	0.7249148516998041
1110	2	0.9924299221226924
1110	3	0.775486406902278
1110	4	1.205970333709942
1110	5	1.0512223273357861
1110	6	0.6857122362958705
1110	7	0.5950974337728852
1110	8	0.5112324870248486
1110	9	0.7175267411567248
1110	10	1.0652488077291054
1110	11	0.7273490650667327
1110	12	0.6835790942551796
1110	13	0.6597038717117272
1110	14	1.608065962064579
1110	15	0.8376441943470141
1110	16	1.3960290907204964
1110	17	1.5730098517133513
1110	18	2.1995508833604163
1110	19	1.3325195519503623
1110	20	0.861283492081192
1110	21	0.9045973521183797
1110	22	1.2029812878570638
1110	23	2.6605512390383126
1110	24	1.568200127698951
1110	25	1.9061584063823744
1111	1	0.3361925587094916
1111	2	0.7193059755035414
1111	3	0.44921720508818197
1111	4	0.8204910079778855
1111	5	0.5756192920359666
1111	6	0.17255192443001732
1111	7	0.1255453046085293
1111	8	-0.12788964133196856
1111	9	0.0705273168529672
1111	10	0.315245395231031
1111	11	0.2801310324890949
1111	12	0.20486198513599163
1111	13	0.03795297365164174
1111	14	-0.5611849815823078
1111	15	-0.27437472808507835
1111	16	0.25047703449503445
1111	17	0.8713016750032594
1111	18	1.5524698716980083
1111	19	0.6139856236440835
1111	20	0.45353957010496515
1111	21	0.41143594616917056
1111	22	0.6443208626754355
1111	23	0.5436629162941458
1111	24	0.5224660889423518
1111	25	0.18651268099208806
1112	1	0.3675656812919428
1112	2	0.7453597547939737
1112	3	0.5237030384421857
1112	4	1.0533768821731115
1112	5	0.7287906678119924
1112	6	0.22461790071390197
1112	7	0.16651642415938284
1112	8	0.26959080083605613
1112	9	0.25430213101149235
1112	10	0.648351192431079
1112	11	0.3090110699296318
1112	12	0.3208722707847542
1112	13	0.2250440789204191
1112	14	0.6994721031096134
1112	15	0.422392443885407
1112	16	0.6960040710677973
1112	17	0.8845841310925096
1112	18	0.8964704365585163
1112	19	0.34178862812972
1112	20	0.34000795989124555
1112	21	0.31035751341417495
1112	22	0.3799529696843891
1112	23	2.0735966635497363
1112	24	1.0340029634337071
1112	25	0.9554709404971149
1113	1	0.39777982444950055
1113	2	0.8216384416042821
1113	3	0.5262902844443751
1113	4	0.6152177932202818
1113	5	0.7878583100828322
1113	6	0.3574912091947411
1113	7	0.23667612529532533
1113	8	0.1879018542879578
1113	9	0.24216446257348423
1113	10	0.5044919205651723
1113	11	0.26794571684153823
1113	12	0.21476388449599426
1113	13	0.15245058405705691
1113	14	0.9575978329606372
1113	15	0.4626765114844815
1113	16	0.7687850622536416
1113	17	1.269358799638141
1113	18	1.7149599722820525
1113	19	0.9641109748560552
1113	20	0.5165321398539717
1113	21	0.6680907408010768
1113	22	0.9236268590074914
1113	23	0.7523251193838831
1113	24	0.6078594924672274
1113	25	0.9641668065233233
1114	1	-0.6411492858885168
1114	2	-3.429497636153819
1114	3	-1.3653650444294883
1114	4	-0.23944228746560015
1114	5	-0.006520572401619518
1114	6	-0.18752801605700853
1114	7	-0.28670243455158584
1114	8	-0.25410647657267305
1114	9	-0.15943241168022837
1114	10	-0.07568507954957227
1114	11	-0.22514986916865354
1114	12	-0.2922013859821391
1114	13	-0.31755006278851994
1114	14	0.41665393115867033
1114	15	-0.10891138054785898
1114	16	0.14062527313904383
1114	17	0.49178239358087694
1114	18	0.6918150719036333
1114	19	-5.3191214234971085E-5
1114	20	-0.09124641869728123
1114	21	-0.2990422403433257
1114	22	-0.04604216905451608
1114	23	1.5957206640803747
1114	24	0.5365733799080488
1114	25	0.5361000100491111
1115	1	-0.11713479788308234
1115	2	0.28047652987280003
1115	3	0.03825477790481813
1115	4	0.2543221353137284
1115	5	0.17875859437030073
1115	6	-0.313537815496133
1115	7	-0.4380687503438949
1115	8	-0.4004809945599419
1115	9	-0.49647185414308836
1115	10	-0.20518498187914247
1115	11	-0.28682223761584424
1115	12	-0.5266708716378972
1115	13	-0.6768627984919533
1115	14	0.26319064183841334
1115	15	-0.3823032557179147
1115	16	0.07207470810644168
1115	17	0.5908952221146038
1115	18	1.0684054463622275
1115	19	0.2845837032941816
1115	20	-0.04342599267679503
1115	21	-0.17290430269928692
1115	22	0.12896945401591609
1115	23	1.7927180648706482
1115	24	0.5916400262371582
1115	25	0.51869085849372
1116	1	0.18402175620168393
1116	2	0.23620373598023647
1116	3	0.22193222658435374
1116	4	0.8466110326542449
1116	5	0.38541963348794894
1116	6	0.19058785818641866
1116	7	0.036837513860248805
1116	8	0.05880195674939159
1116	9	-0.0512950037771418
1116	10	0.5023923582690912
1116	11	0.07196258284369605
1116	12	0.138031223851101
1116	13	-0.04924649838898887
1116	14	0.6835373854355353
1116	15	0.004009926661055374
1116	16	0.33051519677559327
1116	17	0.7188853522594059
1116	18	1.2298918613970642
1116	19	0.3816713539161469
1116	20	0.14048261840081722
1116	21	0.08544676359756664
1116	22	0.4639399093835763
1116	23	1.8738651572521192
1116	24	0.7580678621971941
1116	25	0.9752568167694662
1201	1	1.3036821754397536
1201	2	1.6886690674189424
1201	3	1.4065434304676465
1201	4	1.8906619386524
1201	5	1.7300175611533106
1201	6	1.293546380377407
1201	7	1.1526028152200283
1201	8	1.2638606106653612
1201	9	1.4785412216281486
1201	10	1.7556501139659346
1201	11	1.4232670749765006
1201	12	1.5767793179877785
1201	13	1.6072176713806527
1201	14	2.330727702094073
1201	15	1.7866832176072975
1201	16	2.1197411310375642
1201	17	2.6186797783222815
1201	18	3.1726628570096835
1201	19	2.2238333030308937
1201	20	2.0078303827064587
1201	21	1.9698667069662008
1201	22	2.2235654785495598
1201	23	3.861424926162954
1201	24	2.588383068652753
1201	25	2.7231872825780172
1202	1	1.013596539340572
1202	2	1.2887329539480668
1202	3	1.0742547540090222
1202	4	1.661313831738127
1202	5	1.4027338861176943
1202	6	1.0112460502333889
1202	7	0.8918070533922489
1202	8	1.0012802962568017
1202	9	1.0555482260059748
1202	10	1.5613549090578829
1202	11	1.1529399821499453
1202	12	1.3197402892347867
1202	13	1.2287459287646385
1202	14	2.1597429692568966
1202	15	1.542975551677067
1202	16	1.7235519696568105
1202	17	2.2321171336203633
1202	18	2.9190053055050456
1202	19	2.0585168893696704
1202	20	1.6488634741631998
1202	21	1.625618510424708
1202	22	2.0344998798709923
1202	23	3.616363786659157
1202	24	2.2879214777445895
1202	25	2.566857208221066
1203	1	1.0361613336071542
1203	2	1.291555812486898
1203	3	1.0003612103646944
1203	4	1.5610429337132956
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2211	16	-0.4634166852401478
2211	17	-8.307109697298642E-4
2211	18	-5.568069775505133
2211	19	-2.0140026843702614
2211	20	-1.2114398501811294
2211	21	-0.9401857673891172
2211	22	-0.47966921263638085
2211	23	-5.087888693915932
2211	24	-2.244397268795243
2211	25	-1.6375331831346713
2212	1	0.3462571204651361
2212	2	0.5475954943045593
2212	3	0.3353297727929032
2212	4	0.8027456567083817
2212	5	0.7508606324554421
2212	6	0.3128335960447046
2212	7	0.20190167018159855
2212	8	0.13311140980887615
2212	9	-0.02675175703585353
2212	10	-3.704474864564453
2212	11	-1.1106634878421673
2212	12	-0.1990077449941836
2212	13	-0.031979892154168965
2212	14	0.7743895229292903
2212	15	0.31833072281021657
2212	16	0.4624321304572945
2212	17	1.0509309379070046
2212	18	1.6534238280658258
2212	19	0.7990023346086588
2212	20	0.4667011522337603
2212	21	0.2968857363208315
2212	22	0.61326189316393
2212	23	1.9334289815214873
2212	24	1.0839876946684868
2212	25	0.8823642804255272
2213	1	-1.522202397157841
2213	2	-3.765215873251581
2213	3	-1.7534219100744508
2213	4	-3.3627429030216547
2213	5	-1.250140367086285
2213	6	-0.9773293368579203
2213	7	-0.9512787499633077
2213	8	-0.9342893995502237
2213	9	-0.8461053381265558
2213	10	-0.8118820216747267
2213	11	-0.9855868613428669
2213	12	-0.7362136786004143
2213	13	-1.0990769549551445
2213	14	-6.383209227763302
2213	15	-2.8874689725268823
2213	16	-2.0743125082389327
2213	17	-0.8724184046852699
2213	18	-0.1658794583428076
2213	19	-0.7465745917323332
2213	20	-0.8441058212540682
2213	21	-0.9897869747322687
2213	22	-0.8217722161063179
2213	23	-5.4423355002490865
2213	24	-3.0611012170618466
2213	25	-2.231668862624524
2214	1	-0.44983707756925284
2214	2	-0.27768243055304254
2214	3	-0.40777006159843765
2214	4	-0.1549798838825467
2214	5	-0.38439675961707565
2214	6	-0.6878092437919321
2214	7	-0.6308411202504729
2214	8	-0.7752810618359476
2214	9	-0.6167644580111471
2214	10	-0.34503868968960916
2214	11	-0.6576802659415364
2214	12	-0.6652195963657874
2214	13	-0.7195020877984746
2214	14	-0.22741790396880646
2214	15	-0.617682270864961
2214	16	-0.1256183406174074
2214	17	0.01853891885727421
2214	18	0.6264971697564903
2214	19	-0.37379468372256497
2214	20	-0.5505045904280408
2214	21	-0.6637958287090129
2214	22	-0.2009890675567476
2214	23	1.1617839826921852
2214	24	0.1383264001752238
2214	25	-0.10182849982722786
2215	1	-2.500861496247726
2215	2	-4.197398498247399
2215	3	-2.470972269340926
2215	4	-3.854445647136984
2215	5	-4.99360334975534
2215	6	-2.5489707902889975
2215	7	-1.88820048632288
2215	8	-2.12967528892408
2215	9	-1.8896202487180815
2215	10	-3.142431375821193
2215	11	-2.180359548829223
2215	12	-1.865554528369394
2215	13	-2.070928578784553
2215	14	-1.2432074181538795
2215	15	-1.9278269014682365
2215	16	-2.7301083724946267
2215	17	-7.219626688762482
2215	18	-3.356289806948329
2215	19	-2.4816223005111055
2215	20	-2.295773218976713
2215	21	-2.2734434479215224
2215	22	-2.1108233906805225
2215	23	-6.487512006011318
2215	24	-4.018430626952149
2215	25	-3.8431123801905187
2216	1	-1.025484120409807
2216	2	-0.5815260528593988
2216	3	-0.7939382846198448
2216	4	-3.4849592720438807
2216	5	-1.436933451224725
2216	6	-1.241723428251511
2216	7	-1.244150552167465
2216	8	-1.0377785376903077
2216	9	-1.1093304396766612
2216	10	-0.6700868442650147
2216	11	-1.1877938574796616
2216	12	-1.848554738254812
2216	13	-1.2784056074757046
2216	14	-7.184460861296294
2216	15	-3.3546271332708955
2216	16	-1.7792864760591485
2216	17	-0.8742852163326944
2216	18	-0.260920380752772
2216	19	-0.7189101165697522
2216	20	-1.3740735742960055
2216	21	-1.3222302916349022
2216	22	-0.9055633463631239
2216	23	0.10434852605750677
2216	24	-0.6436480812463472
2216	25	-0.5204054712455033
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86015.0
3	4.0
4	753.0
5	4515.0
6	5882.0
7	5032.0
8	4838.0
9	4292.0
10	4243.0
11	4107.0
12	4100.0
13	4003.0
14	4107.0
15	4118.0
16	4263.0
17	4339.0
18	5044.0
19	5955.0
20	7344.0
21	9439.0
22	11405.0
23	13982.0
24	16541.0
25	19558.0
26	22071.0
27	25216.0
28	28335.0
29	34440.0
30	40224.0
31	45840.0
32	59726.0
33	78030.0
34	102098.0
35	135539.0
36	192516.0
37	271158.0
38	687447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.79762663749426	17.782003092575668	12.138257166803626	25.28211310312645
2	13.883119127755295	19.583574947407392	41.65968670452721	24.873619220310104
3	19.571753021984485	24.269953380501615	28.658413750860245	27.499879846653652
4	10.732072438804794	16.153428092149294	38.8961574466758	34.218342022370116
5	12.312325363206185	38.11559709486181	36.14749635068218	13.424581191249818
6	32.98365057147852	35.77530194452993	17.956357219319134	13.284690264672413
7	28.776572386444794	31.11206424558015	21.464593922966856	18.6467694450082
8	26.00033434369202	36.30481715643246	20.10090635769356	17.593942142181955
9	26.886183766539197	15.14714002331336	19.459038017137363	38.50763819301008
10	14.236104542454203	29.234798196373212	34.6594833823064	21.869613878866183
11	34.67634497982237	22.849499469870548	23.030980621902682	19.443174928404407
12	23.404819830205298	25.105578523275838	31.16086020999659	20.32874143652227
13	29.062611344072803	21.238097526289344	25.249781337493758	24.4495097921441
14	20.887292521680806	21.794246534444927	28.0634811574821	29.25497978639216
15	24.672986348934188	29.003006475603886	22.938882302691415	23.38512487277051
16	22.152366599219466	27.938682008774613	27.866804219420498	22.042147172585423
17	22.341108637837408	27.92918484556922	27.945237834748283	21.78446868184509
18	21.96120310653247	26.976651930131645	29.68714854181918	21.374996421516705
19	23.38875058860702	27.19300532903452	27.772946215250453	21.645297867108003
20	23.585472351037307	26.946485235499416	27.812862172603637	21.65518024085964
21	22.94792143838754	26.644658808324063	27.76165049734965	22.645769255938745
22	22.822465687307115	27.299170278080574	26.75254980478936	23.12581422982295
23	21.06942756601419	26.98819671561227	29.151868039617973	22.79050767875557
24	22.711989373048734	27.846712977213205	27.205895135551916	22.23540251418614
25	22.625740310521408	26.545858351193495	27.22834489472048	23.600056443564615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	171.0
2	326.5
3	482.0
4	482.0
5	482.0
6	1036.0
7	1590.0
8	1590.0
9	1590.0
10	2484.5
11	3379.0
12	3379.0
13	3379.0
14	5724.5
15	8070.0
16	8070.0
17	8070.0
18	14406.5
19	20743.0
20	20743.0
21	20743.0
22	32418.5
23	44094.0
24	44094.0
25	44094.0
26	62342.0
27	80590.0
28	80590.0
29	80590.0
30	104313.0
31	128036.0
32	128036.0
33	128036.0
34	148557.5
35	169079.0
36	169079.0
37	169079.0
38	187766.0
39	206453.0
40	206453.0
41	206453.0
42	223847.0
43	241241.0
44	241241.0
45	241241.0
46	266592.0
47	291943.0
48	291943.0
49	291943.0
50	275849.0
51	259755.0
52	259755.0
53	259755.0
54	227013.5
55	194272.0
56	194272.0
57	194272.0
58	165408.0
59	136544.0
60	136544.0
61	136544.0
62	111369.0
63	86194.0
64	86194.0
65	86194.0
66	66604.5
67	47015.0
68	47015.0
69	47015.0
70	34648.0
71	22281.0
72	22281.0
73	22281.0
74	15581.5
75	8882.0
76	8882.0
77	8882.0
78	6277.5
79	3673.0
80	3673.0
81	3673.0
82	2526.0
83	1379.0
84	1379.0
85	1379.0
86	925.5
87	472.0
88	472.0
89	472.0
90	306.0
91	140.0
92	140.0
93	140.0
94	86.0
95	32.0
96	32.0
97	32.0
98	20.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11045126574288314
2	0.047073399236092266
3	0.03501116012673529
4	0.03097337669606071
5	0.03444893711740085
6	0.0186555816733699
7	0.02044447306670674
8	0.022897809834711545
9	0.028571151110722667
10	0.034857826578734985
11	0.020086694788039372
12	0.020035583605372602
13	0.01574224426136419
14	0.02100669607604117
15	0.02085336252804087
16	0.02151780790270884
17	0.02540225778538312
18	0.019575582961371702
19	0.03220004508006311
20	0.022591142738710945
21	0.028162261649388533
22	0.034908937761401755
23	0.027497816274720564
24	0.03766894162540717
25	0.02979781949472507
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1956519.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.85197759405443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93619668433449	60.30981723492948
2	10.889971782629344	15.649320170507375
3	2.7546249565318477	5.937757519702487
4	1.0445805342235572	3.0022070856086587
5	0.4864248899610133	1.7475295147334564
6	0.2525860512393569	1.0889284376532578
7	0.14114113695111352	0.7098888866867545
8	0.09395232575332228	0.5400528323949613
9	0.06532059112738012	0.4224072285103418
>10	0.30318743192351416	3.711721008201621
>50	0.017324152788120713	0.8572753512301434
>100	0.011000265982041395	1.521427407644733
>500	0.0018807668711333806	1.0073930105739228
>1k	0.001591418121728236	2.0847453128737943
>5k	1.4467437470256693E-4	0.7797394461164963
>10k+	7.233718735128346E-5	0.6297895526325547
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	12117	0.6193142003732138	No Hit
CCCATGTACTCTGCGTTGATACCAC	9897	0.5058473748529915	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5105	0.2609225875138448	No Hit
GCGTTGATACCACTGCTTCCCATGT	3421	0.17485135590300938	No Hit
GTATCAACGCAGAGTACATGGGAAG	3339	0.1706602389243345	No Hit
CATGTACTCTGCGTTGATACCACTG	3251	0.16616245484965902	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2930	0.14975576521362688	No Hit
TATCAACGCAGAGTACATGGGAAGC	2584	0.13207129601092552	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2485	0.1270112889269156	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2206	0.11275126896288766	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2185	0.11167793412688556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	5.1111182666766843E-5
4	0.0	0.0	0.0	5.1111182666766843E-5	5.1111182666766843E-5
5	0.0	0.0	0.0	5.1111182666766843E-5	5.1111182666766843E-5
6	0.0	0.0	0.0	5.1111182666766843E-5	5.1111182666766843E-5
7	0.0	0.0	0.0	5.1111182666766843E-5	5.1111182666766843E-5
8	0.0	0.0	0.0	5.1111182666766843E-5	5.1111182666766843E-5
9	0.0	0.0	0.0	1.0222236533353369E-4	5.1111182666766843E-5
10	0.0	0.0	0.0	1.0222236533353369E-4	5.1111182666766843E-5
11	0.0	0.0	0.0	1.5333354800030054E-4	5.1111182666766843E-5
12	0.0	0.0	0.0	1.5333354800030054E-4	5.1111182666766843E-5
13	0.0	5.1111182666766843E-5	0.0	1.5333354800030054E-4	5.1111182666766843E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAC	25	0.0059883506	19.024246	1
TGCGCGA	35	0.0021723828	16.283566	10
TAGGACC	375	0.0	14.695152	4
GCGTTTA	155	0.0	13.482093	13
GTAGGAC	900	0.0	12.983613	3
TGTAGGA	1055	0.0	12.608841	2
GCCGGTT	100	1.4465877E-7	12.34774	11
ATAGGAC	230	0.0	11.9785185	3
GACGTGG	390	0.0	11.934324	7
TAGGACA	430	0.0	11.93171	4
CTGTAGG	1010	0.0	11.77243	1
TATCGAC	65	8.030488E-4	11.691363	19
GTCCTAC	1000	0.0	11.604789	1
AGGACCG	60	0.0058745197	11.083855	5
ACAGCGC	105	3.4725854E-6	10.855711	8
TGCGTTT	210	0.0	10.855433	12
TGCACCG	80	3.7699708E-4	10.688004	5
ACGTGGA	455	0.0	10.646948	8
TAGGACG	785	0.0	10.529966	4
TCCTACA	1150	0.0	10.49314	2
>>END_MODULE