FastQCFastQC Report
Thu 26 May 2016
SRR1513112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956519
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC142730.7295099102027631No Hit
CCCATGTACTCTGCGTTGATACCAC130470.666847600253307No Hit
GAGTACATGGGAAGCAGTGGTATCA55660.2844848427232243No Hit
GTATCAACGCAGAGTACTTTTTTTT42830.2189091953617624No Hit
CATGTACTCTGCGTTGATACCACTG38600.19728916509372No Hit
GTATCAACGCAGAGTACATGGGAAG37180.19003137715503912No Hit
GCGTTGATACCACTGCTTCCCATGT34320.1754135789123438No Hit
ACGCAGAGTACATGGGAAGCAGTGG32370.16544689829232426No Hit
TATCAACGCAGAGTACATGGGAAGC31050.15870022218031105No Hit
TATCAACGCAGAGTACTTTTTTTTT28710.14674020543628763No Hit
GCTTCCCATGTACTCTGCGTTGATA27640.14127130889094355No Hit
ACTCTGCGTTGATACCACTGCTTCC27210.13907352803627257No Hit
GGTATCAACGCAGAGTACTTTTTTT24850.1270112889269156No Hit
GGTATCAACGCAGAGTACATGGGAA21360.10917348617621397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGT8150.012.2638325
TAAGGCT1850.011.8345194
GCGTGCG1052.769102E-711.7460489
TTAGGAC6000.011.5815353
TAGGACG8550.011.5787524
GGCGTGT752.0999894E-411.3849228
GGACGTG8450.011.3778236
CTTACAC2350.011.341873
GACGTGA4700.011.3041787
TAGGACC4050.011.2818614
CTAGTAC1104.8428774E-711.249813
GGCGTGC1451.2441888E-911.12328
TTTGCGC600.005911075511.0746217
CTGTTCG600.005935876611.0683929
AAGGCGT1900.011.0220816
GACTTAC1303.1268428E-810.9942571
GGCGAGG3900.010.94676119
CGTGAAA5200.010.7643159
CGTGCGC1158.988354E-710.72328310
TGGCGAG8150.010.59712518
Produced by FastQC (version 0.11.5)