##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513112_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956519 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.629555859155982 31.0 31.0 34.0 27.0 34.0 2 30.770965679351953 33.0 31.0 34.0 27.0 34.0 3 30.770931434859563 33.0 31.0 34.0 27.0 34.0 4 34.012472150794345 37.0 35.0 37.0 30.0 37.0 5 33.94605879114897 37.0 35.0 37.0 30.0 37.0 6 33.888108421129566 37.0 35.0 37.0 30.0 37.0 7 33.80874399890826 37.0 35.0 37.0 29.0 37.0 8 33.806483351298915 37.0 35.0 37.0 29.0 37.0 9 35.37380776777532 39.0 37.0 39.0 29.0 39.0 10 35.27961394701508 39.0 35.0 39.0 29.0 39.0 11 35.2891129603137 39.0 35.0 39.0 29.0 39.0 12 35.207247156812684 39.0 35.0 39.0 28.0 39.0 13 35.176314157950934 39.0 35.0 39.0 28.0 39.0 14 36.3704610075343 40.0 37.0 41.0 28.0 41.0 15 36.358097723559034 40.0 37.0 41.0 28.0 41.0 16 36.315141841198574 40.0 37.0 41.0 28.0 41.0 17 36.23543190738245 40.0 37.0 41.0 27.0 41.0 18 36.16010731303913 40.0 37.0 41.0 27.0 41.0 19 36.2268447175826 40.0 37.0 41.0 27.0 41.0 20 36.1350204112508 40.0 37.0 41.0 27.0 41.0 21 36.154706905478555 40.0 37.0 41.0 27.0 41.0 22 36.136754102566854 40.0 37.0 41.0 27.0 41.0 23 36.076078484287656 40.0 37.0 41.0 27.0 41.0 24 35.949844596449104 40.0 37.0 41.0 27.0 41.0 25 35.92797309916234 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2675457844876732 1101 2 0.13220774880822006 1101 3 0.1515158527505207 1101 4 0.4123142445183561 1101 5 0.1956174182329562 1101 6 0.1476136106670225 1101 7 -0.00754232512864661 1101 8 0.10474255568023949 1101 9 0.16756175167181198 1101 10 0.18268324352585807 1101 11 0.05914094607010867 1101 12 0.07145804211928919 1101 13 0.06063629759977118 1101 14 0.028040051562335577 1101 15 0.0759817189069878 1101 16 2.3139835658270158E-4 1101 17 0.03662917418217404 1101 18 -0.014507579721481534 1101 19 0.017425012733788492 1101 20 0.22003275359400476 1101 21 0.1514486082143307 1101 22 0.13638699590944015 1101 23 0.1315194651207463 1101 24 0.16234892453462635 1101 25 0.18310822020986706 1102 1 0.5761376886433069 1102 2 0.5428997907353157 1102 3 0.5127656180300484 1102 4 0.8714388351761713 1102 5 0.5612799283697854 1102 6 0.4990405391751267 1102 7 0.45209125644093007 1102 8 0.5674699766026592 1102 9 0.46673113352986206 1102 10 0.47601092293311353 1102 11 0.48193699822654423 1102 12 0.4731705214071411 1102 13 0.5091453325323627 1102 14 0.4915904557630242 1102 15 0.5039433409922438 1102 16 0.48536370504148607 1102 17 0.4850412257113135 1102 18 0.5833724043484807 1102 19 0.5500558010437828 1102 20 0.5363193622494649 1102 21 0.5554501709539963 1102 22 0.43873791487877867 1102 23 0.4449062012302818 1102 24 0.5801675270519269 1102 25 0.6414803297089406 1103 1 0.047278775349475666 1103 2 0.16647037351979677 1103 3 0.40016798182645985 1103 4 -0.35243049844477525 1103 5 -0.129536382776422 1103 6 0.01890352666029571 1103 7 0.11526047397120465 1103 8 0.18439103378166521 1103 9 0.3329490940683115 1103 10 0.33032755938837255 1103 11 0.36367898466446036 1103 12 0.42457306148509844 1103 13 0.3497367107409488 1103 14 0.44190735664060554 1103 15 0.42502093154818965 1103 16 0.42810537022947415 1103 17 0.4036313908245006 1103 18 0.4402290027872269 1103 19 0.4799848129277109 1103 20 0.5702680573600887 1103 21 0.47329937368589725 1103 22 0.40876981951973335 1103 23 0.416103767617102 1103 24 0.3675384617036599 1103 25 0.42883340425632355 1104 1 0.31330380552545023 1104 2 0.302554485075607 1104 3 0.35836631738328606 1104 4 0.07658290604032203 1104 5 0.10464187781693113 1104 6 0.16373549413376765 1104 7 0.14286990158063162 1104 8 0.14236702005953816 1104 9 0.07301643413565273 1104 10 0.02843441409899583 1104 11 0.06322793327001364 1104 12 0.04035350745422761 1104 13 0.12380443197848479 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0.08235132053498972 1107 12 0.06949447464829461 1107 13 0.08909152115937502 1107 14 0.10694218761298657 1107 15 0.15289161253291184 1107 16 0.056030190874281516 1107 17 0.05648024447482669 1107 18 -0.05504229425397966 1107 19 -0.08453678755982708 1107 20 0.07424195338607831 1107 21 0.10602581436024394 1107 22 0.20358208195597172 1107 23 0.13555429785407114 1107 24 0.2775955641468073 1107 25 0.17823547865422285 1108 1 -0.41360756041594016 1108 2 -0.24570863662862408 1108 3 -0.07439792461883243 1108 4 -0.9244127538467879 1108 5 -0.6204449077779373 1108 6 -0.47111471254091697 1108 7 -0.36698061057934694 1108 8 -0.3327986918267243 1108 9 -0.2003282521420573 1108 10 -0.22461216296605357 1108 11 -0.14333277998815674 1108 12 -0.034419294801253386 1108 13 -0.1383241673325628 1108 14 -0.011809948756514643 1108 15 -0.07454948801002814 1108 16 -0.05458176267335091 1108 17 -0.04376110512784237 1108 18 -0.22810128124744722 1108 19 -0.05765862342155259 1108 20 0.03092078647767238 1108 21 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-0.8986793522557335 2216 24 -0.8591389857439253 2216 25 -0.9475775616050583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48154.0 3 0.0 4 821.0 5 6582.0 6 7834.0 7 5963.0 8 4713.0 9 4171.0 10 3660.0 11 3423.0 12 2983.0 13 2781.0 14 2824.0 15 2814.0 16 2835.0 17 3007.0 18 3299.0 19 4022.0 20 5192.0 21 6794.0 22 8588.0 23 10390.0 24 12134.0 25 13727.0 26 14617.0 27 15958.0 28 18109.0 29 21436.0 30 27039.0 31 34545.0 32 44048.0 33 57377.0 34 77324.0 35 105205.0 36 154793.0 37 259873.0 38 959484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.76208822804988 18.690942719718816 13.125905397745166 27.42106365448614 2 13.529173786580074 19.973332610473523 39.622298929468485 26.875194673477914 3 17.432397759693497 24.261724144628367 29.15925128212467 29.146626813553468 4 10.363661175792313 16.75271234268617 37.29148554141309 35.59214094010843 5 12.67026664478665 38.06964081502032 35.75107495796532 13.509017582227703 6 33.13063515970304 36.16182270356341 17.521422327646704 13.186119809086843 7 28.376086636517527 31.069575404191983 21.558848063067853 18.995489896222633 8 25.930631594231947 36.639183670130414 19.737958848106935 17.692225887530704 9 26.309632518582678 14.951780654384223 19.392091638741952 39.34649518829115 10 14.238654142705483 29.50651251774248 34.279973605429795 21.97485973412224 11 34.52973681372406 22.78073466850878 22.947874386579343 19.74165413118781 12 23.117808255589694 25.20381348069823 31.08914018799554 20.589238075716533 13 28.960053160221843 21.06724256906995 25.34674265852225 24.62596161218596 14 21.068051330987963 21.746872826133067 27.338703865464435 29.846371977414528 15 24.52987862097729 29.166845556080297 22.912104986619074 23.39117083632334 16 22.29763750467086 28.00638354754102 27.406724705422896 22.289254242365224 17 22.592365753455475 28.188604662100953 27.262442050931785 21.95658753351179 18 22.58988294528206 27.199085084857767 28.537649719664028 21.67338225019615 19 23.232843795135295 27.28703810217672 27.84447256413206 21.635645538555927 20 23.49182399966471 27.007404477032935 27.739061056907705 21.761710466394653 21 22.822880840922068 26.70722850123101 27.6883076525196 22.78158300532732 22 22.745089620903247 27.246144811269403 26.52675491523466 23.48201065259269 23 21.872706382160942 27.24787326042823 27.38924700845789 23.490173348952933 24 22.551101143259025 27.820785152352755 27.35439394187207 22.27371976251615 25 22.632145474766908 26.474788374040003 26.972407102822466 23.920659048370627 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 117.0 2 315.0 3 513.0 4 513.0 5 513.0 6 1197.0 7 1881.0 8 1881.0 9 1881.0 10 2549.5 11 3218.0 12 3218.0 13 3218.0 14 5341.0 15 7464.0 16 7464.0 17 7464.0 18 13620.5 19 19777.0 20 19777.0 21 19777.0 22 31517.0 23 43257.0 24 43257.0 25 43257.0 26 61435.5 27 79614.0 28 79614.0 29 79614.0 30 102393.5 31 125173.0 32 125173.0 33 125173.0 34 145566.0 35 165959.0 36 165959.0 37 165959.0 38 183269.0 39 200579.0 40 200579.0 41 200579.0 42 216909.5 43 233240.0 44 233240.0 45 233240.0 46 265342.0 47 297444.0 48 297444.0 49 297444.0 50 281861.5 51 266279.0 52 266279.0 53 266279.0 54 231802.0 55 197325.0 56 197325.0 57 197325.0 58 168289.5 59 139254.0 60 139254.0 61 139254.0 62 114055.5 63 88857.0 64 88857.0 65 88857.0 66 68789.5 67 48722.0 68 48722.0 69 48722.0 70 35924.0 71 23126.0 72 23126.0 73 23126.0 74 16080.5 75 9035.0 76 9035.0 77 9035.0 78 6389.0 79 3743.0 80 3743.0 81 3743.0 82 2536.5 83 1330.0 84 1330.0 85 1330.0 86 898.5 87 467.0 88 467.0 89 467.0 90 298.5 91 130.0 92 130.0 93 130.0 94 71.5 95 13.0 96 13.0 97 13.0 98 7.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18645359436836545 2 0.06756898348546576 3 5.1111182666766843E-5 4 0.0 5 0.020240028336039672 6 0.36549606725004974 7 0.0011755572013356374 8 0.03286449045473108 9 0.033988936473399955 10 0.002862226229338943 11 0.003680005152007213 12 0.010017791802686301 13 0.008637789870683596 14 0.0020444473066706737 15 1.5333354800030054E-4 16 0.012266683840024044 17 0.02509559068938252 18 0.06976676434013675 19 0.030360042504059506 20 0.0 21 0.0 22 0.0 23 1.5333354800030054E-4 24 3.577782786673679E-4 25 5.1111182666766843E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1956519.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.10208154713601 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58024736461242 54.07102329748614 2 12.801354645331017 17.179950866496206 3 3.5311681300694175 7.108461954616975 4 1.3275752789261777 3.563322585058649 5 0.6125744808347939 2.0552511383335426 6 0.31483907111871284 1.2675814214659453 7 0.2020784041355706 0.9491917087254118 8 0.12359276969013261 0.6634665688306942 9 0.08807750247171499 0.5319165377992583 >10 0.3788432284994787 4.366745197354685 >50 0.02200484964590739 1.013243330423429 >100 0.013597427218881103 1.7753414652542883 >500 0.0014507566314750032 0.7015261209518776 >1k 0.0023670239776697336 3.0680239645485186 >5k 7.635561218289462E-5 0.28518071788037835 >10k+ 1.5271122436578925E-4 1.3997731247739735 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 14273 0.7295099102027631 No Hit CCCATGTACTCTGCGTTGATACCAC 13047 0.666847600253307 No Hit GAGTACATGGGAAGCAGTGGTATCA 5566 0.2844848427232243 No Hit GTATCAACGCAGAGTACTTTTTTTT 4283 0.2189091953617624 No Hit CATGTACTCTGCGTTGATACCACTG 3860 0.19728916509372 No Hit GTATCAACGCAGAGTACATGGGAAG 3718 0.19003137715503912 No Hit GCGTTGATACCACTGCTTCCCATGT 3432 0.1754135789123438 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3237 0.16544689829232426 No Hit TATCAACGCAGAGTACATGGGAAGC 3105 0.15870022218031105 No Hit TATCAACGCAGAGTACTTTTTTTTT 2871 0.14674020543628763 No Hit GCTTCCCATGTACTCTGCGTTGATA 2764 0.14127130889094355 No Hit ACTCTGCGTTGATACCACTGCTTCC 2721 0.13907352803627257 No Hit GGTATCAACGCAGAGTACTTTTTTT 2485 0.1270112889269156 No Hit GGTATCAACGCAGAGTACATGGGAA 2136 0.10917348617621397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1111182666766843E-5 0.0 5 0.0 0.0 0.0 5.1111182666766843E-5 0.0 6 0.0 0.0 0.0 5.1111182666766843E-5 0.0 7 0.0 0.0 0.0 5.1111182666766843E-5 0.0 8 0.0 0.0 0.0 5.1111182666766843E-5 0.0 9 0.0 0.0 0.0 1.0222236533353369E-4 5.1111182666766843E-5 10 0.0 0.0 0.0 1.0222236533353369E-4 5.1111182666766843E-5 11 0.0 0.0 0.0 1.5333354800030054E-4 5.1111182666766843E-5 12 0.0 0.0 0.0 1.5333354800030054E-4 5.1111182666766843E-5 13 0.0 0.0 0.0 1.5333354800030054E-4 5.1111182666766843E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACGT 815 0.0 12.263832 5 TAAGGCT 185 0.0 11.834519 4 GCGTGCG 105 2.769102E-7 11.746048 9 TTAGGAC 600 0.0 11.581535 3 TAGGACG 855 0.0 11.578752 4 GGCGTGT 75 2.0999894E-4 11.384922 8 GGACGTG 845 0.0 11.377823 6 CTTACAC 235 0.0 11.34187 3 GACGTGA 470 0.0 11.304178 7 TAGGACC 405 0.0 11.281861 4 CTAGTAC 110 4.8428774E-7 11.24981 3 GGCGTGC 145 1.2441888E-9 11.1232 8 TTTGCGC 60 0.0059110755 11.07462 17 CTGTTCG 60 0.0059358766 11.068392 9 AAGGCGT 190 0.0 11.022081 6 GACTTAC 130 3.1268428E-8 10.994257 1 GGCGAGG 390 0.0 10.946761 19 CGTGAAA 520 0.0 10.764315 9 CGTGCGC 115 8.988354E-7 10.723283 10 TGGCGAG 815 0.0 10.597125 18 >>END_MODULE