FastQCFastQC Report
Thu 26 May 2016
SRR1513111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051781
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81590.3976545255073519No Hit
CCCATGTACTCTGCGTTGATACCAC65540.31942980269336735No Hit
GAGTACATGGGAAGCAGTGGTATCA32570.15874013844557486No Hit
GTATCAACGCAGAGTACTTTTTTTT25440.12398984102104464No Hit
GTATCAACGCAGAGTACATGGGAAG22980.11200025733740589No Hit
GCGTTGATACCACTGCTTCCCATGT22050.10746760984724979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6750.013.2309544
GCGCCAC1051.9987056E-812.66484613
GGACGTG6900.011.7020736
TGTAGGA13650.011.6241582
GTAGGAC12000.011.5586453
GTCCTAC10950.011.46606351
TCCTACA12050.011.1964142
ACCCGCG600.00588303211.0817417
AGGACGT7550.011.0734165
TAGGACG7550.010.9481154
CTGTAGG14100.010.9282461
AAGGCGT1158.827865E-710.7383736
CGTGCGC803.776795E-410.68596410
GTACCGT1002.401268E-510.4492636
GACGTGG4550.010.438067
AGGACCT10050.010.3040115
GACGTGA3150.010.2524957
GTGTAGG3550.010.1814531
TGGCGAG8700.010.15519818
GTCTTAG3750.010.1457281
Produced by FastQC (version 0.11.5)