##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513111_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2051781
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.37867979087437	31.0	30.0	34.0	25.0	34.0
2	29.049858635010267	31.0	30.0	34.0	16.0	34.0
3	29.42967694895313	31.0	30.0	34.0	23.0	34.0
4	32.30197569818611	35.0	33.0	37.0	19.0	37.0
5	32.528414094876595	35.0	33.0	37.0	22.0	37.0
6	32.987127281127954	37.0	35.0	37.0	27.0	37.0
7	32.95986998612425	37.0	35.0	37.0	26.0	37.0
8	32.94904963054049	37.0	35.0	37.0	26.0	37.0
9	34.33484519059295	39.0	35.0	39.0	26.0	39.0
10	33.83475429395242	39.0	34.0	39.0	22.0	39.0
11	34.222189892586	39.0	35.0	39.0	26.0	39.0
12	34.06657143233123	39.0	35.0	39.0	25.0	39.0
13	34.15449260910399	39.0	35.0	39.0	25.0	39.0
14	34.4778697141654	40.0	34.0	41.0	17.0	41.0
15	35.024425121394536	40.0	34.0	41.0	24.0	41.0
16	34.61384231552977	39.0	34.0	41.0	17.0	41.0
17	34.147067352704795	39.0	33.0	41.0	16.0	41.0
18	33.56596829778617	39.0	32.0	41.0	10.0	41.0
19	34.461715943368226	39.0	34.0	41.0	18.0	41.0
20	34.72157213659742	39.0	34.0	41.0	18.0	41.0
21	34.74430653173999	40.0	35.0	41.0	18.0	41.0
22	34.33772122853267	40.0	34.0	41.0	11.0	41.0
23	32.663543526331516	39.0	31.0	41.0	8.0	41.0
24	33.763291501383435	39.0	33.0	41.0	2.0	41.0
25	33.50623726411347	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0093739885161348
1101	2	1.2548077619441926
1101	3	1.0127964361919055
1101	4	1.2173930465395202
1101	5	1.372799037757325
1101	6	1.1119232955747833
1101	7	1.0732990367076098
1101	8	1.1074797894740342
1101	9	1.2278176270736765
1101	10	1.5435028715374912
1101	11	1.236287352443206
1101	12	1.262362486723994
1101	13	1.1518371741411997
1101	14	0.34477661324515907
1101	15	0.9583064547214946
1101	16	1.6212890977233556
1101	17	2.1193598546237737
1101	18	-3.98720805495649
1101	19	-0.35010851498689277
1101	20	0.5708943263082702
1101	21	0.9031332282272189
1101	22	1.5239378780607424
1101	23	3.3932544344917943
1101	24	2.3101773549113034
1101	25	2.3914145665877555
1102	1	0.8492762257710744
1102	2	1.219830742949906
1102	3	0.8890056918982303
1102	4	1.4786143023564762
1102	5	1.2959589951858703
1102	6	0.8699662378400674
1102	7	0.8269819899947564
1102	8	0.7014820901354497
1102	9	0.8094136673956811
1102	10	1.144726647224033
1102	11	0.852091441802294
1102	12	0.9889638075044118
1102	13	0.8546339220753651
1102	14	1.671543057186767
1102	15	1.2287431604119945
1102	16	1.452084157549777
1102	17	1.1093004533079096
1102	18	1.6052036548569717
1102	19	1.3312090080427694
1102	20	1.1636411992627984
1102	21	1.2416087685907158
1102	22	1.5825516834633007
1102	23	3.0645248482523257
1102	24	1.990718635189431
1102	25	2.2921493236815422
1103	1	0.6114032726211178
1103	2	0.8847540247678367
1103	3	0.6606103361942992
1103	4	1.0733132501137348
1103	5	1.0249550349366174
1103	6	0.7121774508574106
1103	7	0.5832481744024847
1103	8	0.5621929637797649
1103	9	0.6488390813210358
1103	10	1.016405543605309
1103	11	0.7787121188338801
1103	12	0.8859397639917788
1103	13	0.7151650667460103
1103	14	1.6132311512189759
1103	15	1.0630384302684206
1103	16	1.2806612300132656
1103	17	1.7285740252390482
1103	18	1.739193054160502
1103	19	1.1903774840777288
1103	20	1.1451955815645505
1103	21	1.036149770194612
1103	22	1.4490043594579305
1103	23	2.834165518682809
1103	24	1.8094611369918994
1103	25	1.95514983677878
1104	1	0.5226884419007227
1104	2	0.9252829641198161
1104	3	0.7768761409285894
1104	4	1.2694326944318846
1104	5	1.0239318346820099
1104	6	0.6291151247146587
1104	7	0.6082480710697951
1104	8	0.5084868336715189
1104	9	0.697295497660825
1104	10	1.0644529691747735
1104	11	0.749684107409422
1104	12	0.8114106510408874
1104	13	0.5755737681848174
1104	14	1.420491512387379
1104	15	0.9711746392815073
1104	16	1.2025125797448055
1104	17	1.7558976673442217
1104	18	2.3433728613003737
1104	19	1.4605221911709023
1104	20	0.7841648173573361
1104	21	0.9348556316378804
1104	22	1.318915948011977
1104	23	2.8216157639946076
1104	24	1.7920648726751551
1104	25	1.8612195987415987
1105	1	0.4003376912615586
1105	2	0.8170828825329934
1105	3	0.4917463492173262
1105	4	0.7582272289225145
1105	5	0.8082552496964084
1105	6	0.4474630723213764
1105	7	0.22200999102585683
1105	8	0.2495637797393968
1105	9	0.49536689824809343
1105	10	0.9490393199415692
1105	11	0.5106635935739874
1105	12	0.5670478158583876
1105	13	0.5962859161396921
1105	14	1.298892612597811
1105	15	0.7452669858720284
1105	16	1.1213351041703703
1105	17	1.699466646579971
1105	18	1.4819224333734482
1105	19	1.0447403914948268
1105	20	0.785226657746044
1105	21	0.6833707110638656
1105	22	1.1001840980509527
1105	23	2.5677646235966094
1105	24	1.6096440058353494
1105	25	1.6472335169689885
1106	1	-0.5736217401182131
1106	2	-0.13328692241704587
1106	3	-0.2362829464223708
1106	4	-2.713555250624598
1106	5	-0.5073537512970105
1106	6	-0.5162215155569285
1106	7	-0.6934670598206836
1106	8	-0.5549746572522594
1106	9	-0.47442935628730964
1106	10	-0.3464067798385031
1106	11	-0.3966429663622222
1106	12	-0.36077151621218206
1106	13	-0.508321387590108
1106	14	0.15185125728071824
1106	15	-0.42476392417791686
1106	16	-1.195204561676242
1106	17	-6.07673019498387
1106	18	-7.213023757053133
1106	19	-3.245862067363557
1106	20	-1.852241441655032
1106	21	-1.431924882695391
1106	22	-1.6620429267500967
1106	23	-5.506580691209287
1106	24	-2.4095369598740177
1106	25	-1.0225869783301107
1107	1	0.48872081567974845
1107	2	0.8462747464662677
1107	3	0.5031418284987694
1107	4	0.8857413085598935
1107	5	0.8747039165206942
1107	6	0.5328707016893262
1107	7	0.37547185556258
1107	8	0.4598615765593195
1107	9	0.29976079372490005
1107	10	0.8315590037346254
1107	11	0.5745204002241877
1107	12	0.677538914005801
1107	13	0.6006728867992379
1107	14	1.3235227036987283
1107	15	0.7938328067547076
1107	16	-5.572486178167292
1107	17	-0.9855314293100861
1107	18	0.6901980846277809
1107	19	0.6720348554991276
1107	20	0.5150470972345218
1107	21	0.5232237268712439
1107	22	0.6980508586313263
1107	23	2.523799354522943
1107	24	1.5350311773146004
1107	25	1.1780841714136798
1108	1	0.38056697141574247
1108	2	0.7134222890608868
1108	3	0.3950656188362096
1108	4	0.8795570737889236
1108	5	0.7103539220484762
1108	6	0.3282249095584291
1108	7	0.3205355478740515
1108	8	0.32656296587473577
1108	9	0.3917184877806932
1108	10	0.8579847410790791
1108	11	0.40080953997875923
1108	12	0.47793003516245847
1108	13	0.3394525986176333
1108	14	0.9351978667682417
1108	15	0.6381724827039505
1108	16	0.8749269435544278
1108	17	0.9563831480604108
1108	18	0.7312353418661033
1108	19	0.7580215642588186
1108	20	0.5264868997085088
1108	21	0.6975458367635099
1108	22	1.0301913379512868
1108	23	2.5922878137463528
1108	24	1.4201314758147277
1108	25	1.5982966838511885
1109	1	0.2690640294082982
1109	2	0.6206897230653325
1109	3	0.4912853542397997
1109	4	0.48574130855988784
1109	5	0.3024730272851244
1109	6	0.3035415285224019
1109	7	0.1873283298215469
1109	8	0.11758388545167975
1109	9	0.25015080932552536
1109	10	0.7317150099748773
1109	11	0.31523802892933617
1109	12	0.35616605909160626
1109	13	0.32922202876491724
1109	14	-0.8845896207942516
1109	15	-0.1475088469114425
1109	16	-0.2966671454059835
1109	17	0.7308492259161241
1109	18	1.5332558069366726
1109	19	0.828353108229237
1109	20	0.6080268164232834
1109	21	0.5107432276512753
1109	22	0.3719978165096478
1109	23	-4.034078960609662
1109	24	-1.191957902248582
1109	25	0.13377839918278767
1110	1	0.516400384965209
1110	2	0.791578729774745
1110	3	0.6662073511196738
1110	4	1.1784627973705426
1110	5	0.7850172068029195
1110	6	0.4145055441001375
1110	7	0.3263818601779249
1110	8	0.2964951030250731
1110	9	0.37568071560734495
1110	10	0.648282567542708
1110	11	0.3554967933141455
1110	12	0.3282397977868783
1110	13	0.21076952293852713
1110	14	0.9900202682467878
1110	15	0.4324110711566789
1110	16	0.8863569726247533
1110	17	1.1491081543172683
1110	18	1.776835711502045
1110	19	0.9986900855225116
1110	20	0.7474479531709548
1110	21	0.5763546271683779
1110	22	0.82498052995021
1110	23	2.324642799018669
1110	24	1.3046627889849347
1110	25	1.6143544779716663
1111	1	0.5257709445250534
1111	2	0.8814002183996443
1111	3	0.6527315146884476
1111	4	1.2163237511884546
1111	5	0.8461687899855193
1111	6	0.44103116193673486
1111	7	0.36025834739400153
1111	8	0.11110208714628556
1111	9	0.2866545296973868
1111	10	0.5440668572086196
1111	11	0.3273832210567278
1111	12	0.5202220860167017
1111	13	0.310984860616486
1111	14	-0.3121606790269169
1111	15	0.08541784562094534
1111	16	0.5864528634092423
1111	17	1.0757210282231568
1111	18	1.6925654358274045
1111	19	0.8517624535249837
1111	20	0.6304808893073925
1111	21	0.564344420026103
1111	22	0.9204944924361627
1111	23	0.9678166022016939
1111	24	0.6704664385912196
1111	25	0.18087208218661743
1112	1	0.40643560309801074
1112	2	0.7699212028653122
1112	3	0.6191175252718288
1112	4	1.0383757549093318
1112	5	0.7610789425574822
1112	6	0.21211529402114593
1112	7	0.19766096677505374
1112	8	0.15067703685875955
1112	9	0.23216056817600617
1112	10	0.70862065265338
1112	11	0.29756933318059
1112	12	0.39379878713216954
1112	13	0.29497434538370726
1112	14	0.7228233686675836
1112	15	0.42675941804463946
1112	16	0.8195853873717738
1112	17	1.0717110877049834
1112	18	1.24418582433902
1112	19	0.691176006113075
1112	20	0.5386151453643251
1112	21	0.6602626969726657
1112	22	0.6064618018518289
1112	23	2.224719424322984
1112	24	1.0708988375022201
1112	25	1.131257799733696
1113	1	0.3907767466310581
1113	2	0.757022655959048
1113	3	0.4558820863692965
1113	4	0.6850705525314638
1113	5	0.7358347611493343
1113	6	0.33040009151847727
1113	7	0.31427445456517944
1113	8	0.18676333039044124
1113	9	0.28900367962793183
1113	10	0.6201603995302492
1113	11	0.3092979910252467
1113	12	0.31067879330748127
1113	13	0.308160950315731
1113	14	1.031269912225497
1113	15	0.579633642721582
1113	16	0.9072549600410227
1113	17	1.3955287994833299
1113	18	1.8567202311414732
1113	19	0.9483759545288706
1113	20	0.5971233727212422
1113	21	0.5575427395712325
1113	22	0.7184784714998358
1113	23	0.5099306121786924
1113	24	0.7229349113156971
1113	25	1.029849449286523
1114	1	-0.413504721720205
1114	2	-3.1869314208164248
1114	3	-1.1901226102013602
1114	4	0.012757588258978103
1114	5	0.1568873169183007
1114	6	-0.05757796594497222
1114	7	-0.03482316379547257
1114	8	-0.13949573900077894
1114	9	-0.005452395463187543
1114	10	0.14822400713928374
1114	11	-0.1369445498941957
1114	12	-0.03768197569857534
1114	13	-0.1871441444776636
1114	14	0.5307357527086367
1114	15	-0.049382917708847174
1114	16	0.37806703824877985
1114	17	0.7219932144307961
1114	18	0.9171766276195399
1114	19	0.34265652713779104
1114	20	0.13710269412664644
1114	21	0.05572654319930592
1114	22	0.24365058681564022
1114	23	1.7967108505721896
1114	24	0.7656155485621028
1114	25	0.7965582623558944
1115	1	0.0811739045434976
1115	2	0.4033967234715661
1115	3	0.07349871060065283
1115	4	0.23122543167002618
1115	5	0.21390463642209312
1115	6	-0.14473106236354027
1115	7	-0.22098310850445557
1115	8	-0.3116562201596267
1115	9	-0.2534055900817336
1115	10	-0.17480803832540914
1115	11	-0.23960573250027295
1115	12	-0.37793121868921276
1115	13	-0.43013767856351137
1115	14	0.4486699803063985
1115	15	-0.264210991847726
1115	16	0.304326767544282
1115	17	0.8226111671103737
1115	18	1.3167334993324857
1115	19	0.45261628813420174
1115	20	-0.062243528451254804
1115	21	0.03318222488677236
1115	22	0.3406974716028941
1115	23	2.0297871693820397
1115	24	0.8422563687109275
1115	25	0.4586847885377452
1116	1	0.52301764822721
1116	2	0.44668330213433904
1116	3	0.5489860892354912
1116	4	1.258994890388557
1116	5	0.7419063349545141
1116	6	0.6086183608091744
1116	7	0.502874704485528
1116	8	0.4615070356361102
1116	9	0.5371479970782005
1116	10	1.000291775517411
1116	11	0.5918209574150168
1116	12	0.7126649932062747
1116	13	0.6126348034781515
1116	14	1.235034669795347
1116	15	0.6775639762262031
1116	16	1.1205441497873991
1116	17	1.3306698929001044
1116	18	1.900934514084959
1116	19	1.0389600943064323
1116	20	0.7882297998235188
1116	21	0.7119326684873357
1116	22	1.0554191308903071
1116	23	2.3840516522553017
1116	24	1.2324146681598478
1116	25	1.5170020876808934
1201	1	1.105954106197924
1201	2	1.42560983285588
1201	3	1.1051493418909324
1201	4	1.6406130327630706
1201	5	1.4251903947800884
1201	6	0.9875914587463726
1201	7	0.9685141006538345
1201	8	1.0451708689573849
1201	9	1.1273166171180193
1201	10	1.4538858928550198
1201	11	1.0357820042809678
1201	12	1.312831700799677
1201	13	1.1790070445557959
1201	14	2.0936582553304177
1201	15	1.4879452937327642
1201	16	1.8739406413313304
1201	17	2.2451208659972792
1201	18	2.7733463255211603
1201	19	1.963908442110359
1201	20	1.6358222538835037
1201	21	1.5663991994405748
1201	22	1.8340140506197855
1201	23	3.4718777628273685
1201	24	2.291492170098408
1201	25	2.454417705967984
1202	1	1.0836719514215147
1202	2	1.432671165673824
1202	3	1.1662073511196738
1202	4	1.7265832930246106
1202	5	1.5653139335915327
1202	6	1.0978924282128375
1202	7	0.9528715745879879
1202	8	1.0248022370129632
1202	9	1.1057695864326291
1202	10	1.6207644541515975
1202	11	1.3060401950473945
1202	12	1.2673407207377565
1202	13	1.1714649043438143
1202	14	1.9359161130476679
1202	15	1.3461996501195372
1202	16	1.724502942235219
1202	17	2.0415326925217983
1202	18	2.7599592153871484
1202	19	1.8843621051336186
1202	20	1.6732046512853742
1202	21	1.6057000808992257
1202	22	1.9574243016318746
1202	23	3.4331599331345686
1202	24	2.190341209087599
1202	25	2.3692276522314444
1203	1	1.1852470462304368
1203	2	1.4900708678125874
1203	3	1.213739120425636
1203	4	1.8469191887151766
1203	5	1.553032062354248
1203	6	1.1704285050767353
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2211	15	-0.37704599640726144
2211	16	-0.5130858544459471
2211	17	0.07889362939020117
2211	18	-5.339244050301545
2211	19	-1.808949034733132
2211	20	-0.9401234467142743
2211	21	-0.8128343491029781
2211	22	-0.30499555790389365
2211	23	-4.476638864174678
2211	24	-2.023452351814324
2211	25	-1.3399106757256405
2212	1	0.17991538258235096
2212	2	0.5315318908704221
2212	3	0.28400251690833045
2212	4	0.81704973536241
2212	5	0.5804145570938246
2212	6	0.13407417839982827
2212	7	0.14220360943143362
2212	8	-0.09990592724898306
2212	9	-0.1736329148774871
2212	10	-3.61012922840575
2212	11	-1.1068998705314783
2212	12	-0.4067635321546419
2212	13	-0.20481996384134504
2212	14	0.591537266859504
2212	15	0.23575898829530217
2212	16	0.39656860385665027
2212	17	0.8548493864183584
2212	18	1.3917531675934143
2212	19	0.6656287858073142
2212	20	0.20110153166476152
2212	21	0.12968587565778478
2212	22	0.18275272330957648
2212	23	1.6395526973929435
2212	24	0.742197215428078
2212	25	0.7295990563040888
2213	1	-1.5255922425831514
2213	2	-3.7574136218596337
2213	3	-1.7117825292851094
2213	4	-3.296068392531005
2213	5	-1.3051853912871287
2213	6	-0.9725977451994225
2213	7	-0.8691598984288333
2213	8	-0.7805095470252752
2213	9	-0.8134772518962805
2213	10	-0.8050606027239624
2213	11	-0.8918008616419257
2213	12	-0.8331586362513121
2213	13	-1.1506613947228956
2213	14	-6.137275047438546
2213	15	-2.73180957680632
2213	16	-1.9679891224800983
2213	17	-0.9626592209534905
2213	18	-0.29829649103380973
2213	19	-0.95426011350294
2213	20	-1.1021050736729237
2213	21	-1.3172185826334655
2213	22	-1.1243203692444652
2213	23	-5.4102418194515245
2213	24	-2.856575007631971
2213	25	-2.1851150998874367
2214	1	0.022179042358331458
2214	2	0.181068397921063
2214	3	-0.0355523576181902
2214	4	0.37958010337381864
2214	5	-0.0015114743279482923
2214	6	-0.20932908096217062
2214	7	-0.39397117944037774
2214	8	-0.37744322631240834
2214	9	-0.19039774951003352
2214	10	0.15880358138181094
2214	11	-0.2865899325386394
2214	12	-0.2922730265300686
2214	13	-0.37343641980603337
2214	14	0.2855752270617984
2214	15	-0.12825296259839547
2214	16	0.26892931340382376
2214	17	0.5454387439628618
2214	18	1.0305632793326538
2214	19	0.02604264445146498
2214	20	-0.027198502783654988
2214	21	-0.11777562184057189
2214	22	0.4050523959743799
2214	23	1.7329616918902246
2214	24	0.7626039549829855
2214	25	0.5739387410333592
2215	1	-2.485160503209368
2215	2	-4.495896122186661
2215	3	-2.548827613915293
2215	4	-3.9750285767809785
2215	5	-4.955073183654221
2215	6	-2.343281798956152
2215	7	-1.700878471177763
2215	8	-2.26265550594216
2215	9	-1.737265323683431
2215	10	-3.20170332675859
2215	11	-2.1784345361550876
2215	12	-1.9462556741413621
2215	13	-1.8004829776171576
2215	14	-1.1147793738389211
2215	15	-1.826521825612403
2215	16	-2.568463471804492
2215	17	-6.939847511044267
2215	18	-3.304776441626
2215	19	-2.403691355367485
2215	20	-2.1938588044560916
2215	21	-2.1334419943030625
2215	22	-1.9080869323348537
2215	23	-6.941637172029029
2215	24	-4.169260903459541
2215	25	-3.797961506777085
2216	1	-0.6484788584364374
2216	2	-0.2304363053782943
2216	3	-0.47835400039188514
2216	4	-2.8747377887308545
2216	5	-0.8604166299727716
2216	6	-0.5959198939179018
2216	7	-0.6718266101756925
2216	8	-0.47640890330551855
2216	9	-0.5510018983121228
2216	10	-0.33878667909090865
2216	11	-0.8451110477486097
2216	12	-1.5470991598396182
2216	13	-0.8199230810821518
2216	14	-6.756753927639199
2216	15	-2.9783126350795612
2216	16	-1.42912217686154
2216	17	-0.5668672755121662
2216	18	0.2291077240009045
2216	19	-0.24148340425912096
2216	20	-0.7267659042719075
2216	21	-0.6959116822914169
2216	22	-0.2501834595973591
2216	23	0.6856330335606771
2216	24	-0.03800471151870255
2216	25	-0.007427870289092198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93746.0
3	3.0
4	813.0
5	4828.0
6	5820.0
7	5227.0
8	4843.0
9	4654.0
10	4334.0
11	4329.0
12	4255.0
13	4181.0
14	4315.0
15	4272.0
16	4423.0
17	4748.0
18	5300.0
19	6299.0
20	7818.0
21	9650.0
22	12153.0
23	14658.0
24	17609.0
25	20288.0
26	23518.0
27	26781.0
28	30127.0
29	36057.0
30	42678.0
31	48478.0
32	62756.0
33	82651.0
34	108685.0
35	143055.0
36	201903.0
37	283384.0
38	713142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38837049079862	18.069740753246197	12.394338845636641	25.14754991031854
2	13.977876440627965	19.481410504911604	41.891547259423966	24.649165795036463
3	19.26919693964517	24.19458957661711	28.72154320518146	27.814670278556257
4	10.66956143143615	16.25321358381207	39.0528990146066	34.02432597014518
5	12.503169185763042	37.92764505119454	36.13193564115066	13.437250121891761
6	32.87263084002808	36.18882107479916	17.89027766944856	13.048270415724202
7	28.974947205447343	30.92975935823663	21.538715030311774	18.55657840600425
8	25.81053247370538	36.514753019463505	19.956222349514327	17.71849215731679
9	26.629451770957758	15.329449333300833	19.65590034858494	38.38519854715648
10	14.182678243368166	29.20225878296415	34.6313241827465	21.98373879092118
11	34.64038523224399	22.74515022907045	23.385611509388525	19.22885302929703
12	23.27900674625943	24.892083735708866	31.22885852744449	20.600050990587214
13	28.830064623497563	21.371819826354514	25.453819571896748	24.344295978251175
14	20.75378246465182	21.724626433475617	28.208976906442135	29.312614195430424
15	24.159997738024202	29.52419534110985	22.97991911486547	23.33588780600048
16	22.26185566407221	28.12565507303181	27.60442412560242	22.00806513729356
17	22.164662475953122	28.063203916532842	27.927332364145514	21.844801243368522
18	21.7078172750171	27.06049185738096	29.727152674289876	21.50453819331207
19	23.377250226832714	27.23986639175338	27.686754870991788	21.696128510422117
20	23.577949985959876	27.02582407725188	27.82245483760257	21.573771099185674
21	23.101895751926342	26.646174247317987	27.432507867480187	22.81942213327548
22	22.91000523650576	27.397505394966124	27.01105712081896	22.68143224770915
23	21.19291115740454	27.00877110444932	29.12732117862661	22.670996559519534
24	22.756525432034238	27.406546373681884	27.5673021648154	22.26962602946848
25	22.645396882663533	26.713584351887516	27.52377980703131	23.117238958417637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	172.0
2	333.0
3	494.0
4	494.0
5	494.0
6	964.0
7	1434.0
8	1434.0
9	1434.0
10	2336.0
11	3238.0
12	3238.0
13	3238.0
14	5866.0
15	8494.0
16	8494.0
17	8494.0
18	15394.0
19	22294.0
20	22294.0
21	22294.0
22	34872.5
23	47451.0
24	47451.0
25	47451.0
26	66988.5
27	86526.0
28	86526.0
29	86526.0
30	110528.0
31	134530.0
32	134530.0
33	134530.0
34	158279.5
35	182029.0
36	182029.0
37	182029.0
38	203081.5
39	224134.0
40	224134.0
41	224134.0
42	239835.5
43	255537.0
44	255537.0
45	255537.0
46	274609.5
47	293682.0
48	293682.0
49	293682.0
50	280350.0
51	267018.0
52	267018.0
53	267018.0
54	235265.5
55	203513.0
56	203513.0
57	203513.0
58	173969.0
59	144425.0
60	144425.0
61	144425.0
62	117581.0
63	90737.0
64	90737.0
65	90737.0
66	69764.0
67	48791.0
68	48791.0
69	48791.0
70	35792.0
71	22793.0
72	22793.0
73	22793.0
74	16035.5
75	9278.0
76	9278.0
77	9278.0
78	6393.0
79	3508.0
80	3508.0
81	3508.0
82	2359.0
83	1210.0
84	1210.0
85	1210.0
86	789.5
87	369.0
88	369.0
89	369.0
90	230.0
91	91.0
92	91.0
93	91.0
94	57.5
95	24.0
96	24.0
97	24.0
98	16.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11234142435279398
2	0.05000533682688357
3	0.03786953870807849
4	0.03518894073002918
5	0.038064491288300265
6	0.02051875906834111
7	0.02110361680900642
8	0.02466150139805369
9	0.03075376952998395
10	0.03728468096741319
11	0.021785950839782606
12	0.020567497213396558
13	0.01754573221995915
14	0.0230531426112241
15	0.023248095191445873
16	0.023540524061778523
17	0.02821938598710096
18	0.020177592052953018
19	0.03460408298936388
20	0.02519762099366355
21	0.030022697354152316
22	0.03874682531907645
23	0.029925221064041437
24	0.041134994426793116
25	0.032703295332201635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2051781.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.38564596860925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11673778947116	63.314635232105466
2	10.257286385892096	15.259897473992098
3	2.488523293526159	5.553312380906231
4	0.8737444112068388	2.599761697563314
5	0.42041869286351646	1.5636558022965503
6	0.22233056432745257	0.9922921587637795
7	0.13009242563656256	0.6773906381619278
8	0.09178198782636204	0.5461809962197577
9	0.06649900771707633	0.4451914480775629
>10	0.3046597375096889	3.913625342297753
>50	0.01598175463335669	0.8147926367635486
>100	0.009092439953886089	1.3291844515877695
>500	0.0015915401502907998	0.7924086208867575
>1k	0.0011273409397892181	1.4719049326841616
>5k	1.3262834585755506E-4	0.7257661876933567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8159	0.3976545255073519	No Hit
CCCATGTACTCTGCGTTGATACCAC	6554	0.31942980269336735	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3257	0.15874013844557486	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2544	0.12398984102104464	No Hit
GTATCAACGCAGAGTACATGGGAAG	2298	0.11200025733740589	No Hit
GCGTTGATACCACTGCTTCCCATGT	2205	0.10746760984724979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.873814505544208E-5	0.0	0.0	0.0
6	0.0	4.873814505544208E-5	0.0	0.0	0.0
7	0.0	4.873814505544208E-5	0.0	4.873814505544208E-5	0.0
8	0.0	4.873814505544208E-5	0.0	4.873814505544208E-5	0.0
9	0.0	4.873814505544208E-5	0.0	9.747629011088415E-5	0.0
10	0.0	4.873814505544208E-5	0.0	9.747629011088415E-5	9.747629011088415E-5
11	0.0	4.873814505544208E-5	0.0	9.747629011088415E-5	9.747629011088415E-5
12	0.0	9.747629011088415E-5	0.0	1.4621443516632624E-4	1.949525802217683E-4
13	0.0	9.747629011088415E-5	0.0	1.4621443516632624E-4	1.949525802217683E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	675	0.0	13.230954	4
GCGCCAC	105	1.9987056E-8	12.664846	13
GGACGTG	690	0.0	11.702073	6
TGTAGGA	1365	0.0	11.624158	2
GTAGGAC	1200	0.0	11.558645	3
GTCCTAC	1095	0.0	11.4660635	1
TCCTACA	1205	0.0	11.196414	2
ACCCGCG	60	0.005883032	11.081741	7
AGGACGT	755	0.0	11.073416	5
TAGGACG	755	0.0	10.948115	4
CTGTAGG	1410	0.0	10.928246	1
AAGGCGT	115	8.827865E-7	10.738373	6
CGTGCGC	80	3.776795E-4	10.685964	10
GTACCGT	100	2.401268E-5	10.449263	6
GACGTGG	455	0.0	10.43806	7
AGGACCT	1005	0.0	10.304011	5
GACGTGA	315	0.0	10.252495	7
GTGTAGG	355	0.0	10.181453	1
TGGCGAG	870	0.0	10.155198	18
GTCTTAG	375	0.0	10.145728	1
>>END_MODULE