FastQCFastQC Report
Thu 26 May 2016
SRR1513111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051781
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC94610.4611115903695375No Hit
CCCATGTACTCTGCGTTGATACCAC89150.43450056316926605No Hit
GTATCAACGCAGAGTACTTTTTTTT39180.19095605232722204No Hit
GAGTACATGGGAAGCAGTGGTATCA34220.16678193237972277No Hit
TATCAACGCAGAGTACTTTTTTTTT26380.1285712266562562No Hit
GTATCAACGCAGAGTACATGGGAAG24210.11799504917922526No Hit
CATGTACTCTGCGTTGATACCACTG24150.11770262030889261No Hit
GCGTTGATACCACTGCTTCCCATGT22830.11126918516157426No Hit
GGTATCAACGCAGAGTACTTTTTTT22670.11048937484068719No Hit
ACGCAGAGTACATGGGAAGCAGTGG21720.10585925106042018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACG350.002142411416.3175184
CCGTCCG350.00219011516.2638139
TACACGG852.6370253E-713.4379585
GCCGGTT500.001515674313.28146711
TAGGACC6050.013.2158434
TTCGCCT655.4906726E-513.14448917
CTACACG951.0160675E-612.0234364
TGCTCGA658.136377E-411.67316910
CGTCCGA658.136377E-411.67316910
GTAGGAC9950.011.5753263
GTCCTAC12250.011.4354111
TATACCG752.0371731E-411.4222645
TCGGTTT600.005905803311.0759913
AAAGGCG1408.103598E-910.87834555
CTGCGAA700.001500024510.84994912
TCCAACG1053.5103621E-610.8459818
GGCGAGG3000.010.75035319
TTATACT3550.010.725134
TCCTACA14500.010.7088122
TGTAGGA11050.010.6038722
Produced by FastQC (version 0.11.5)