##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513111_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2051781 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.55248683948238 31.0 31.0 34.0 27.0 34.0 2 30.70267684514088 31.0 31.0 34.0 27.0 34.0 3 30.703367952037766 33.0 31.0 34.0 27.0 34.0 4 33.93474303544092 37.0 35.0 37.0 30.0 37.0 5 33.86267881416194 37.0 35.0 37.0 30.0 37.0 6 33.815689393751086 37.0 35.0 37.0 30.0 37.0 7 33.72836428449235 37.0 35.0 37.0 28.0 37.0 8 33.71878431470025 37.0 35.0 37.0 28.0 37.0 9 35.28102609391548 39.0 35.0 39.0 29.0 39.0 10 35.187880675374224 39.0 35.0 39.0 28.0 39.0 11 35.19494819378871 39.0 35.0 39.0 28.0 39.0 12 35.118865512449915 39.0 35.0 39.0 28.0 39.0 13 35.08440325746266 39.0 35.0 39.0 27.0 39.0 14 36.26281411125262 40.0 37.0 41.0 27.0 41.0 15 36.25101801800484 40.0 37.0 41.0 27.0 41.0 16 36.212117180147395 40.0 37.0 41.0 27.0 41.0 17 36.13196340155211 40.0 36.0 41.0 27.0 41.0 18 36.05472416403115 40.0 36.0 41.0 27.0 41.0 19 36.127137350428725 40.0 37.0 41.0 27.0 41.0 20 36.02889440929612 40.0 37.0 41.0 27.0 41.0 21 36.04890580427443 40.0 37.0 41.0 27.0 41.0 22 36.022641305285504 40.0 37.0 41.0 27.0 41.0 23 35.9566206139934 40.0 37.0 41.0 27.0 41.0 24 35.83111160499098 40.0 37.0 41.0 27.0 41.0 25 35.809904175933006 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6777494424506578 1101 2 0.5375063957823016 1101 3 0.6083316889101802 1101 4 0.9297717600996336 1101 5 0.8450080797145318 1101 6 0.8101048102865249 1101 7 0.8252124166862629 1101 8 0.7599315689679145 1101 9 0.8112411726263531 1101 10 0.826632305648701 1101 11 0.8570040919284736 1101 12 0.7386734878141823 1101 13 0.755019072299568 1101 14 0.7698732427336665 1101 15 0.8165749589216063 1101 16 0.8390259946159091 1101 17 0.7697048964122288 1101 18 0.7415288348928328 1101 19 0.7464856960574195 1101 20 0.8828829468446031 1101 21 0.8558458069937771 1101 22 0.9175455225517268 1101 23 0.8333440173478692 1101 24 0.8435814149082006 1101 25 0.8869859377214411 1102 1 0.452637303132601 1102 2 0.4236679071304188 1102 3 0.4136663347609506 1102 4 0.7276886679123606 1102 5 0.4074337512444117 1102 6 0.3391866660987688 1102 7 0.3793169871765443 1102 8 0.42160547940601134 1102 9 0.4076229062351686 1102 10 0.3836446622286829 1102 11 0.406440143067492 1102 12 0.4350467194240011 1102 13 0.44644659890416705 1102 14 0.4135763857075858 1102 15 0.4227198555198868 1102 16 0.5018058819593065 1102 17 0.5677190138279116 1102 18 0.5108239167425026 1102 19 0.5877197765764706 1102 20 0.6849322359033536 1102 21 0.7261726012996874 1102 22 0.49162497629316704 1102 23 0.35816268405004337 1102 24 0.45810682410531456 1102 25 0.428667448741777 1103 1 0.07937770285149526 1103 2 0.1725759707909198 1103 3 0.3619209552242957 1103 4 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25 0.002265185010521975 2210 1 0.23199596304346315 2210 2 0.13786867958847182 2210 3 0.21505154276689709 2210 4 0.5754790242717718 2210 5 0.41443283392336383 2210 6 0.1430867689893489 2210 7 0.021069111771332416 2210 8 0.045441158877125076 2210 9 -0.009103150709712793 2210 10 0.03791566603182872 2210 11 -0.04162239326900874 2210 12 0.061696955930379715 2210 13 0.1306256596565305 2210 14 0.006798422152421324 2210 15 0.042210926672098026 2210 16 0.057609874523741667 2210 17 0.04949778075364719 2210 18 0.044122788928511625 2210 19 0.012963237287905827 2210 20 0.1063670301093822 2210 21 0.11945423502618979 2210 22 0.08049905293452042 2210 23 0.0203327427148281 2210 24 0.08455439436771428 2210 25 -0.010094816459620404 2211 1 0.2937996218497112 2211 2 0.23632463815049576 2211 3 0.3648593013549011 2211 4 0.5979117027176173 2211 5 0.36281344198607 2211 6 0.2936869455277886 2211 7 0.3030033223614126 2211 8 0.2807964170724375 2211 9 0.2637530803095345 2211 10 0.25594206735679137 2211 11 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8 -0.06310476867175652 2214 9 -0.10971686396452185 2214 10 -0.2583433056277755 2214 11 -0.25530432412912063 2214 12 -0.37716866338031707 2214 13 -0.2738192296842499 2214 14 -0.24500631359415337 2214 15 -0.3399126947740996 2214 16 -0.27173757502051643 2214 17 -0.3410880877389957 2214 18 -0.2846158962078533 2214 19 -0.3872745698030471 2214 20 -0.27262185298085484 2214 21 -0.4529278999363626 2214 22 -0.4140387510071548 2214 23 -0.349900617912148 2214 24 -0.23608458425027123 2214 25 -0.26998257511185386 2215 1 -0.19553233195471265 2215 2 -0.11650897087351453 2215 3 -0.09605652587129043 2215 4 0.19654721311104595 2215 5 -0.159615106088431 2215 6 -0.24054566283585643 2215 7 -0.37791142576342196 2215 8 -0.5510812945730379 2215 9 -0.6029084418041606 2215 10 -0.6863028864999805 2215 11 -0.7321010912778334 2215 12 -0.8339942003029464 2215 13 -0.7662661031990865 2215 14 -0.9033737350076905 2215 15 -0.9210090862467055 2215 16 -1.0689417866664783 2215 17 -0.9240678102818265 2215 18 -0.8580488346286543 2215 19 -0.951211644055654 2215 20 -0.6960594590640952 2215 21 -0.912870869588069 2215 22 -0.9413014357334362 2215 23 -0.9314885283835537 2215 24 -0.914922534326081 2215 25 -1.0200858294626798 2216 1 -1.1815753345952267 2216 2 -0.7449536589655068 2216 3 -2.453285569303656 2216 4 -0.932285745078758 2216 5 -1.0382432207123955 2216 6 -0.7816663678569782 2216 7 -0.7774144124033384 2216 8 -0.9149244016734102 2216 9 -0.9642302113209453 2216 10 -1.0454503089254104 2216 11 -1.10255446224987 2216 12 -1.048767152109093 2216 13 -1.041663423720891 2216 14 -1.1514804001268004 2216 15 -1.2098898113307541 2216 16 -1.349343093520062 2216 17 -1.2578768090285806 2216 18 -1.2942956790705225 2216 19 -1.319877038135985 2216 20 -1.1372365392453219 2216 21 -1.2434821670043448 2216 22 -1.3447897178524215 2216 23 -1.3626990882248151 2216 24 -1.2966868626671726 2216 25 -1.4008789644051944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53332.0 3 0.0 4 906.0 5 7165.0 6 8161.0 7 6222.0 8 5012.0 9 4472.0 10 3890.0 11 3583.0 12 3291.0 13 3028.0 14 3021.0 15 3053.0 16 3039.0 17 3338.0 18 3676.0 19 4291.0 20 5571.0 21 7188.0 22 9101.0 23 11040.0 24 12987.0 25 14126.0 26 15475.0 27 17080.0 28 19031.0 29 22935.0 30 28911.0 31 37026.0 32 47038.0 33 61265.0 34 81838.0 35 111742.0 36 163568.0 37 274559.0 38 991820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.08590761586591 19.054899038776103 13.522759121763816 27.336434223594175 2 13.577701725610346 19.847128315353448 39.86915385542057 26.70601610361563 3 17.13486965714177 24.22480761835693 29.261553742821476 29.37876898167982 4 10.299003646100632 16.875095343996264 37.443908487309315 35.381992522593784 5 12.794015348684617 37.917334712566806 35.699706685398766 13.588943253349816 6 32.95249546066431 36.57105038662618 17.48563848104436 12.990815671665153 7 28.628362492153048 30.969483641297778 21.56576428340267 18.836389583146502 8 25.73507831247178 36.92148081410258 19.561493060938737 17.781947812486898 9 26.035234666192125 15.070364510836843 19.585735493794807 39.30866532917622 10 14.142301301787722 29.487351325743266 34.288695760672645 22.081651611796364 11 34.53303998253153 22.634103687875786 23.33069811512037 19.50215821447232 12 23.015593324764342 25.03851903627589 31.210874724541977 20.735012914417794 13 28.823098759607095 21.269219071089605 25.4751395003334 24.4325426689699 14 20.845528296782966 21.68603645207593 27.522267739254826 29.94616751188628 15 24.001317880726923 29.621952461741735 22.922400511945977 23.45432914558537 16 22.40518478043112 28.20208984164602 27.143472011066795 22.24925336685607 17 22.31894754699983 28.38397803522087 27.212711647923932 22.084362769855368 18 22.12307417683847 27.415615914691926 28.6257954102627 21.835514498206898 19 23.254600776843493 27.320816275587838 27.676962987556557 21.74761996001211 20 23.476677091755892 27.004782674174287 27.740972355236742 21.777567878833075 21 22.824901877929467 26.74642176723539 27.468233695506488 22.96044265932865 22 22.929396460928338 27.364957566134006 26.771229483068616 22.934416489869044 23 22.071404445999736 27.258469122131697 27.35423976387297 23.315886667995596 24 22.562898101201352 27.457011528089637 27.68920118278307 22.290889187925945 25 22.6156202830614 26.739159783622128 27.175268705578226 23.469951227738242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 113.0 1 113.0 2 268.0 3 423.0 4 423.0 5 423.0 6 1033.0 7 1643.0 8 1643.0 9 1643.0 10 2396.0 11 3149.0 12 3149.0 13 3149.0 14 5610.0 15 8071.0 16 8071.0 17 8071.0 18 15031.0 19 21991.0 20 21991.0 21 21991.0 22 34053.5 23 46116.0 24 46116.0 25 46116.0 26 65499.0 27 84882.0 28 84882.0 29 84882.0 30 108391.5 31 131901.0 32 131901.0 33 131901.0 34 155544.5 35 179188.0 36 179188.0 37 179188.0 38 199456.0 39 219724.0 40 219724.0 41 219724.0 42 235276.0 43 250828.0 44 250828.0 45 250828.0 46 272883.0 47 294938.0 48 294938.0 49 294938.0 50 283703.0 51 272468.0 52 272468.0 53 272468.0 54 239766.5 55 207065.0 56 207065.0 57 207065.0 58 177091.0 59 147117.0 60 147117.0 61 147117.0 62 120371.5 63 93626.0 64 93626.0 65 93626.0 66 72078.5 67 50531.0 68 50531.0 69 50531.0 70 37001.5 71 23472.0 72 23472.0 73 23472.0 74 16367.0 75 9262.0 76 9262.0 77 9262.0 78 6416.0 79 3570.0 80 3570.0 81 3570.0 82 2395.0 83 1220.0 84 1220.0 85 1220.0 86 801.5 87 383.0 88 383.0 89 383.0 90 235.5 91 88.0 92 88.0 93 88.0 94 50.0 95 12.0 96 12.0 97 12.0 98 6.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1835965924238503 2 0.06989050000950395 3 0.0 4 0.0 5 0.01949525802217683 6 0.3620756796168792 7 0.0014134062066078203 8 0.031143674690427486 9 0.032946986057478846 10 0.003119241283548293 11 0.004142742329712576 12 0.009650152720977532 13 0.00843169909459148 14 0.0022419546725503353 15 9.747629011088415E-5 16 0.011599678523195213 17 0.024222858092554714 18 0.06940311855894951 19 0.032167175736591766 20 0.0 21 0.0 22 0.0 23 1.949525802217683E-4 24 4.873814505544208E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2051781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.56736386370163 #Duplication Level Percentage of deduplicated Percentage of total 1 81.80002076712935 56.90611808765237 2 12.128973131053522 16.875613742021216 3 3.2874261760823806 6.860927188997406 4 1.1575395159524335 3.22107890771504 5 0.5329235854602561 1.8537044490631058 6 0.2988333998550183 1.2473431117404656 7 0.17643498532685414 0.8591881775764084 8 0.1107737674750005 0.6164991190789149 9 0.08456565671145351 0.5294708829736711 >10 0.38656409878699505 4.7139780636466 >50 0.020694537199084238 0.9746066754659342 >100 0.012019524120385759 1.6065125798017803 >500 0.0016154274236796502 0.8271243615059363 >1k 0.0014749554737944458 2.0099591053230093 >5k 1.404719498851853E-4 0.8978755474381471 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9461 0.4611115903695375 No Hit CCCATGTACTCTGCGTTGATACCAC 8915 0.43450056316926605 No Hit GTATCAACGCAGAGTACTTTTTTTT 3918 0.19095605232722204 No Hit GAGTACATGGGAAGCAGTGGTATCA 3422 0.16678193237972277 No Hit TATCAACGCAGAGTACTTTTTTTTT 2638 0.1285712266562562 No Hit GTATCAACGCAGAGTACATGGGAAG 2421 0.11799504917922526 No Hit CATGTACTCTGCGTTGATACCACTG 2415 0.11770262030889261 No Hit GCGTTGATACCACTGCTTCCCATGT 2283 0.11126918516157426 No Hit GGTATCAACGCAGAGTACTTTTTTT 2267 0.11048937484068719 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2172 0.10585925106042018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.873814505544208E-5 2 0.0 0.0 0.0 0.0 4.873814505544208E-5 3 0.0 0.0 0.0 0.0 4.873814505544208E-5 4 0.0 0.0 0.0 0.0 4.873814505544208E-5 5 0.0 0.0 0.0 0.0 4.873814505544208E-5 6 0.0 0.0 0.0 0.0 9.747629011088415E-5 7 0.0 0.0 0.0 0.0 9.747629011088415E-5 8 0.0 0.0 0.0 4.873814505544208E-5 9.747629011088415E-5 9 0.0 0.0 0.0 9.747629011088415E-5 9.747629011088415E-5 10 0.0 0.0 0.0 9.747629011088415E-5 9.747629011088415E-5 11 0.0 0.0 0.0 9.747629011088415E-5 9.747629011088415E-5 12 0.0 0.0 0.0 1.4621443516632624E-4 1.949525802217683E-4 13 0.0 0.0 0.0 1.4621443516632624E-4 3.411670153880945E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGACG 35 0.0021424114 16.317518 4 CCGTCCG 35 0.002190115 16.263813 9 TACACGG 85 2.6370253E-7 13.437958 5 GCCGGTT 50 0.0015156743 13.281467 11 TAGGACC 605 0.0 13.215843 4 TTCGCCT 65 5.4906726E-5 13.144489 17 CTACACG 95 1.0160675E-6 12.023436 4 TGCTCGA 65 8.136377E-4 11.673169 10 CGTCCGA 65 8.136377E-4 11.673169 10 GTAGGAC 995 0.0 11.575326 3 GTCCTAC 1225 0.0 11.435411 1 TATACCG 75 2.0371731E-4 11.422264 5 TCGGTTT 60 0.0059058033 11.07599 13 AAAGGCG 140 8.103598E-9 10.8783455 5 CTGCGAA 70 0.0015000245 10.849949 12 TCCAACG 105 3.5103621E-6 10.84598 18 GGCGAGG 300 0.0 10.750353 19 TTATACT 355 0.0 10.72513 4 TCCTACA 1450 0.0 10.708812 2 TGTAGGA 1105 0.0 10.603872 2 >>END_MODULE