FastQCFastQC Report
Thu 26 May 2016
SRR1513110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488486
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114650.770245739630739No Hit
CCCATGTACTCTGCGTTGATACCAC92980.6246615688693075No Hit
GAGTACATGGGAAGCAGTGGTATCA47650.32012393801486877No Hit
GCGTTGATACCACTGCTTCCCATGT31280.21014641723200622No Hit
CATGTACTCTGCGTTGATACCACTG30380.2041000049714945No Hit
GTATCAACGCAGAGTACATGGGAAG30360.20396564025459427No Hit
GCTTCCCATGTACTCTGCGTTGATA25610.17205401999078257No Hit
ACGCAGAGTACATGGGAAGCAGTGG23710.1592893718852579No Hit
TATCAACGCAGAGTACATGGGAAGC22270.1496151122684392No Hit
ACTCTGCGTTGATACCACTGCTTCC21270.14289687642342622No Hit
GGTATCAACGCAGAGTACATGGGAA14940.10037044352449401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC456.7616336E-414.77790819
AAGATCG400.00527512314.2515635
TTACCCG400.005282185514.24868910
GCGTTTA1700.013.40917913
GTAGGAC4700.012.9371553
TGTAGGA5050.012.6049192
GTCCTAG2500.012.5536131
TAGGACC3150.012.0656264
TGCACCG802.8629522E-511.8763035
GGCGAGA2400.011.87510519
GTGTAGG2300.011.5777721
GCACCGT1251.8335413E-811.3989526
CGTTTAT1950.011.20296814
TGCGTTT2050.011.11980612
TAGGACT1658.0035534E-1110.941334
TAGGACG3150.010.8590644
ACCGTTC700.001492481910.8561458
TTAGGAC2300.010.7399473
GGACCGT803.7736766E-410.6865176
CACCGTT803.7736766E-410.6865177
Produced by FastQC (version 0.11.5)