##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513110_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1488486
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.52218428658382	31.0	28.0	34.0	16.0	34.0
2	28.173914299496268	31.0	28.0	34.0	16.0	34.0
3	28.558720068579753	31.0	28.0	34.0	16.0	34.0
4	31.373208078544238	35.0	32.0	37.0	16.0	37.0
5	31.56810477223165	35.0	32.0	37.0	17.0	37.0
6	32.03397143137389	35.0	32.0	37.0	19.0	37.0
7	31.994946542997383	35.0	32.0	37.0	19.0	37.0
8	31.974056860460898	35.0	32.0	37.0	18.0	37.0
9	33.2475333997095	38.0	33.0	39.0	17.0	39.0
10	32.71491636468197	37.0	32.0	39.0	11.0	39.0
11	33.11937566090645	38.0	33.0	39.0	17.0	39.0
12	32.96818243503802	38.0	33.0	39.0	16.0	39.0
13	33.03654720299687	38.0	33.0	39.0	17.0	39.0
14	33.26792458914629	39.0	32.0	41.0	10.0	41.0
15	33.77684371905413	39.0	33.0	41.0	11.0	41.0
16	33.318942872153315	38.0	32.0	41.0	10.0	41.0
17	32.8252895895561	38.0	32.0	41.0	8.0	41.0
18	32.176954301216135	38.0	30.0	40.0	8.0	41.0
19	33.080625548376	38.0	32.0	41.0	2.0	41.0
20	33.36791746781629	39.0	32.0	41.0	2.0	41.0
21	33.39320893847843	39.0	33.0	41.0	2.0	41.0
22	32.95516786855906	39.0	32.0	41.0	2.0	41.0
23	31.206592470469996	38.0	28.0	40.0	2.0	41.0
24	32.263267508058526	38.0	32.0	41.0	2.0	41.0
25	31.984823505226117	38.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9367038324631842
1101	2	1.3387712670536907
1101	3	1.0199100310622562
1101	4	1.0716632577559935
1101	5	1.3488425858585806
1101	6	0.965381988707037
1101	7	1.0193059502540507
1101	8	1.0902675788917406
1101	9	1.226444230691527
1101	10	1.5383196758851767
1101	11	1.1532991361482274
1101	12	1.1202321927918248
1101	13	1.1396820607867824
1101	14	0.09567927171652713
1101	15	0.6697552799840807
1101	16	1.4274457421076931
1101	17	1.9925616178627408
1101	18	-4.106683096331317
1101	19	-0.5979035034529474
1101	20	0.3705624149420217
1101	21	0.9719749918726421
1101	22	1.50350691405297
1101	23	3.302231958296158
1101	24	2.328839883851245
1101	25	2.447358875896427
1102	1	0.9344940650921281
1102	2	1.1986519772569828
1102	3	1.0648731508640878
1102	4	1.6375657501100989
1102	5	1.294908651924647
1102	6	0.8871533453171097
1102	7	0.8714375288372409
1102	8	0.6939464651512708
1102	9	0.9352243472135768
1102	10	1.3373133239110828
1102	11	0.9089072788531425
1102	12	1.0572643401465527
1102	13	0.9814484354563788
1102	14	1.9573075359254375
1102	15	1.2386939882776247
1102	16	1.7519826292252318
1102	17	1.230052380514799
1102	18	1.645052417984843
1102	19	1.4567605615336987
1102	20	1.3944176065391503
1102	21	1.4482103055273114
1102	22	1.810088887731716
1102	23	3.4615084698307292
1102	24	2.3747340729712434
1102	25	2.6210431592581287
1103	1	0.9013256093966255
1103	2	1.2265443485417968
1103	3	0.9483297489783169
1103	4	1.395654243391398
1103	5	1.3257997410335527
1103	6	0.9189583434887325
1103	7	0.8894198375870559
1103	8	0.7698792365490306
1103	9	0.95906800507165
1103	10	1.2874430690034018
1103	11	0.9666375960643876
1103	12	0.9565643739367431
1103	13	1.0232273735184876
1103	14	1.796517678538578
1103	15	1.3390234658256617
1103	16	1.482144127843533
1103	17	1.9473132454512196
1103	18	1.97512782103707
1103	19	1.5653015194163018
1103	20	1.524221947422049
1103	21	1.3662910224349076
1103	22	1.7646939358943143
1103	23	3.2804600480180035
1103	24	2.0438371515820464
1103	25	2.3594357642970394
1104	1	0.8834992247401203
1104	2	1.2993292551891074
1104	3	1.1177730920139908
1104	4	1.6759055387059476
1104	5	1.5604326230744086
1104	6	1.0283802557782806
1104	7	0.9913499379959632
1104	8	1.0684609974754835
1104	9	1.2359366614822136
1104	10	1.6218180478858812
1104	11	1.2594713729950513
1104	12	1.2701690258003566
1104	13	1.1058270651159177
1104	14	2.167420993437034
1104	15	1.494932417846833
1104	16	1.8522615513104412
1104	17	2.2732633147714836
1104	18	2.9470284126199005
1104	19	2.1465143491532785
1104	20	1.5592170371986853
1104	21	1.7344962257557768
1104	22	2.092753109154067
1104	23	3.551117185119441
1104	24	2.5447708884967213
1104	25	2.7162589657553653
1105	1	1.0152588036905676
1105	2	1.305542040289982
1105	3	0.9623620273935636
1105	4	1.241918734908019
1105	5	1.304563824338441
1105	6	1.0012514172488807
1105	7	0.7586928124857408
1105	8	0.8398428314842334
1105	9	0.9554709174768306
1105	10	1.531010950904033
1105	11	1.0322990104067173
1105	12	0.9702750928347257
1105	13	1.0978842901096968
1105	14	1.854382063177944
1105	15	1.256436850717371
1105	16	1.7629364668225591
1105	17	2.2268163775485377
1105	18	2.104282117651138
1105	19	1.6804034981299196
1105	20	1.43960670553804
1105	21	1.2069403759759538
1105	22	1.992359929630119
1105	23	3.344509026004257
1105	24	2.4692668872092867
1105	25	2.2500151651906464
1106	1	-0.424926901260573
1106	2	0.09063775147831521
1106	3	-0.22155901273236722
1106	4	-2.4584465863073426
1106	5	-0.4378045263699306
1106	6	-0.4087878622069958
1106	7	-0.28503992385926225
1106	8	-0.2926651877619584
1106	9	-0.19630742461826856
1106	10	-0.0691349548252731
1106	11	-0.13968820335074383
1106	12	-0.06413489855201249
1106	13	-0.22265203410545098
1106	14	0.47398615002340705
1106	15	-0.16651787024255782
1106	16	-1.2896850099067763
1106	17	-5.989458608343529
1106	18	-7.03640850280091
1106	19	-3.084707583280281
1106	20	-1.732066792338344
1106	21	-1.3185630544793554
1106	22	-1.5459312284084312
1106	23	-5.155573022764642
1106	24	-2.353521879905866
1106	25	-0.9944051472629454
1107	1	0.825834896124519
1107	2	1.1887190973773052
1107	3	0.8266941489110913
1107	4	1.0419979228714453
1107	5	1.1497729525509506
1107	6	0.7623494303508878
1107	7	0.6175537656208689
1107	8	0.6351932382224064
1107	9	0.8426946594680516
1107	10	1.1981488459439902
1107	11	0.9298828776588977
1107	12	0.8820704999328441
1107	13	0.8900761769428982
1107	14	1.6667101893027692
1107	15	1.0920183423306469
1107	16	-5.578614717397439
1107	17	-0.8170852494041654
1107	18	0.8430563239717799
1107	19	1.0020956009302893
1107	20	0.7652978004916093
1107	21	0.8525724399022394
1107	22	1.0146135470802662
1107	23	2.907079215335475
1107	24	1.928209727897503
1107	25	1.7755271873632807
1108	1	0.29022155379699655
1108	2	0.5419777576165039
1108	3	0.3495746710717249
1108	4	0.8127573896547204
1108	5	0.5762539052239184
1108	6	0.2517356658653114
1108	7	0.04969430248148399
1108	8	0.12361784212706084
1108	9	0.29083703071651
1108	10	0.8482470088010103
1108	11	0.2506312794573802
1108	12	0.24207160198755417
1108	13	0.0641944220807531
1108	14	0.709192620862062
1108	15	0.49165928579613194
1108	16	0.6830248022844572
1108	17	0.6700847896387856
1108	18	0.5504666642667218
1108	19	0.6318615653925832
1108	20	0.3056880167342939
1108	21	0.2717871192709751
1108	22	0.528024042018373
1108	23	2.221791912338869
1108	24	1.0129131196486227
1108	25	1.2951886432664281
1109	1	0.2663673350238831
1109	2	0.4771087792229771
1109	3	0.396474543140787
1109	4	0.3021478387078602
1109	5	0.20930117528913073
1109	6	0.17037184322960286
1109	7	0.061451506418300994
1109	8	-0.023207955144542325
1109	9	-0.002599717978590377
1109	10	0.662209131550334
1109	11	0.40272242762759447
1109	12	0.38561069181518093
1109	13	0.12003770469382857
1109	14	-1.0422824550302465
1109	15	-0.07776648622927951
1109	16	-0.5200627568026732
1109	17	0.6914558027382398
1109	18	1.4901693089323018
1109	19	0.7356308441000934
1109	20	0.6198787296789021
1109	21	0.556675969096986
1109	22	0.15484711212214108
1109	23	-3.98864254285872
1109	24	-1.604974316318092
1109	25	-0.3707356478055708
1110	1	0.5576619999329786
1110	2	0.9507491086826967
1110	3	0.715333444508925
1110	4	1.2942758204848346
1110	5	0.7907392167086975
1110	6	0.6379237164813247
1110	7	0.5373114973293696
1110	8	0.5250258421383762
1110	9	0.5759486354284888
1110	10	1.2084582530004937
1110	11	0.6740807150693371
1110	12	0.8159561559576431
1110	13	0.6271387866156033
1110	14	1.3891454954153133
1110	15	0.8974509989588668
1110	16	1.2586876983496538
1110	17	1.653567723636293
1110	18	2.172355493753784
1110	19	1.402218711785551
1110	20	1.047772016570228
1110	21	0.9047665874549367
1110	22	1.1488669751107068
1110	23	2.539117514949151
1110	24	1.719398631968133
1110	25	1.9913214431148063
1111	1	0.3739171705548152
1111	2	0.7067233740271277
1111	3	0.45052795854342165
1111	4	0.9749535349483978
1111	5	0.6712867364081241
1111	6	0.4195082784669566
1111	7	0.24345517565798502
1111	8	-0.03375337699872816
1111	9	0.12224610892752708
1111	10	0.3398243531740448
1111	11	-0.004329185846472683
1111	12	0.36531900751477053
1111	13	0.1262700109882786
1111	14	-0.6763228792065732
1111	15	-0.17375160743748808
1111	16	0.30971984270049546
1111	17	0.8601232106439127
1111	18	1.5629736548812687
1111	19	0.5811263957777086
1111	20	0.482384579461808
1111	21	0.4251447775392876
1111	22	0.6795709508447345
1111	23	0.30550301928365187
1111	24	0.38110257852297735
1111	25	0.09848410042190636
1112	1	0.30849256471703157
1112	2	0.6500846568554515
1112	3	0.3777088597913192
1112	4	0.9869668503851656
1112	5	0.819393701758532
1112	6	0.12754165668066264
1112	7	0.10043442091740928
1112	8	0.16414544346727666
1112	9	0.011164582272343182
1112	10	0.4833751965220898
1112	11	0.17491127985339006
1112	12	0.1922020031337297
1112	13	0.13129161053586813
1112	14	0.465972737940227
1112	15	0.2707898693907609
1112	16	0.7308637780838723
1112	17	0.9102122419087166
1112	18	0.8613089821258768
1112	19	0.4661722001576436
1112	20	0.5128882956399963
1112	21	0.504434361541314
1112	22	0.5653952143133623
1112	23	2.0918583787365286
1112	24	1.178285675157504
1112	25	1.1200589846430518
1113	1	0.3483603616207489
1113	2	0.7698050551416777
1113	3	0.5342959223616788
1113	4	0.6260555246017105
1113	5	0.7297617756317187
1113	6	0.2021643115464613
1113	7	0.16827098006310592
1113	8	-0.11739986750140119
1113	9	0.17234243103738578
1113	10	0.507373604806709
1113	11	0.08145744990852677
1113	12	0.04013442258234079
1113	13	0.13560078819082122
1113	14	0.7548592862957975
1113	15	0.37770627134425183
1113	16	0.7521491913456941
1113	17	1.1952731064658337
1113	18	1.667312484449532
1113	19	0.8173440628082744
1113	20	0.4818680583122088
1113	21	0.43816924333788876
1113	22	0.7103014501813902
1113	23	0.5204543771805525
1113	24	0.3577639553901335
1113	25	0.7124138711955297
1114	1	-0.5906564948971429
1114	2	-3.3122665839841616
1114	3	-1.3716287604765363
1114	4	-0.1988512127584805
1114	5	0.1147007517167431
1114	6	-0.06391544059038878
1114	7	-0.10191637096826867
1114	8	-0.13003404140988195
1114	9	0.02563902988632094
1114	10	0.16930670687220584
1114	11	0.021048203897294115
1114	12	0.017780290339921123
1114	13	0.02340314450786707
1114	14	0.7109846870219414
1114	15	0.20850061872941694
1114	16	0.4494435641055219
1114	17	0.8475660728671954
1114	18	1.1657981761499556
1114	19	0.4074359018864584
1114	20	0.2283388727184814
1114	21	0.138829758727411
1114	22	0.41470382524988025
1114	23	1.918896974961168
1114	24	1.0557910108023734
1114	25	0.9659741945117446
1115	1	-0.1487574806650862
1115	2	0.12083011936406152
1115	3	-0.06909825612469334
1115	4	0.05273310222566252
1115	5	0.043269449843119645
1115	6	-0.40083945513799435
1115	7	-0.7123254457312029
1115	8	-0.5670152149912262
1115	9	-0.560571939238784
1115	10	-0.2830516892849744
1115	11	-0.3447694317382073
1115	12	-0.5833675784575547
1115	13	-0.6020301011335363
1115	14	0.44759766266354006
1115	15	-0.3850609660724089
1115	16	0.01285384876472051
1115	17	0.5504649554711989
1115	18	1.009653039085478
1115	19	0.09849348826060833
1115	20	-0.15346309737490316
1115	21	-0.18354845376354945
1115	22	0.03025432805103634
1115	23	1.6941766845860649
1115	24	0.4838413107520694
1115	25	0.2703134126410802
1116	1	-0.2526416283439943
1116	2	-0.20668993826830473
1116	3	-0.3249539578852598
1116	4	0.3439989013213314
1116	5	-0.20226072162524034
1116	6	-0.2107325414678165
1116	7	-0.31663821399637726
1116	8	-0.40799999594196024
1116	9	-0.4007155774427602
1116	10	0.06718284465553381
1116	11	-0.3764106863690202
1116	12	-0.28366922271052175
1116	13	-0.5612614415488295
1116	14	0.11611900213305404
1116	15	-0.49886657355656894
1116	16	-0.1440550925021853
1116	17	0.054991631568860555
1116	18	0.5763928865126502
1116	19	-0.050452970387567575
1116	20	-0.29861435214657206
1116	21	-0.36913170979090637
1116	22	-0.04441701252525121
1116	23	1.408183119931774
1116	24	0.3676567583989794
1116	25	0.5358050283797056
1201	1	1.3603552679256445
1201	2	1.703237533094761
1201	3	1.4195357535226236
1201	4	1.9756110750896312
1201	5	1.9219077770340043
1201	6	1.4675202349397765
1201	7	1.294259756703923
1201	8	1.4184174385376593
1201	9	1.6472024467253306
1201	10	1.9260938392498872
1201	11	1.518310942104911
1201	12	1.77924272188109
1201	13	1.4853956033507174
1201	14	2.4758817464071896
1201	15	1.947913077826918
1201	16	2.2853249936877376
1201	17	2.7942874156515316
1201	18	3.302716742202378
1201	19	2.4494301068727893
1201	20	2.136736913399986
1201	21	2.0970201720359327
1201	22	2.4147156480963474
1201	23	4.03140263628573
1201	24	2.90879610553975
1201	25	3.1092660023704823
1202	1	1.3583510075886203
1202	2	1.6277496864668919
1202	3	1.362643136838841
1202	4	1.9938677603952755
1202	5	1.7836043040985565
1202	6	1.4465175341904128
1202	7	1.2849859159340156
1202	8	1.4084672692653442
1202	9	1.516902701584776
1202	10	2.0424839643972916
1202	11	1.6655786206016217
1202	12	1.6676663971825505
1202	13	1.6347770937079034
1202	14	2.557335586416322
1202	15	1.9447014684085318
1202	16	2.1763585058030657
1202	17	2.6480794959893146
1202	18	3.313341338040942
1202	19	2.4816553721928827
1202	20	2.0653713232291935
1202	21	2.1378923999639667
1202	22	2.4324124033932435
1202	23	4.103598231401559
1202	24	2.9141169621718035
1202	25	3.1484671891786533
1203	1	1.1802116352930199
1203	2	1.374457167146371
1203	3	1.1262534763887544
1203	4	1.7963987960508518
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2211	15	-0.3525004078317764
2211	16	-0.43350258025141386
2211	17	-0.04520572861829919
2211	18	-5.259934866180597
2211	19	-1.7787072473272296
2211	20	-1.0144031671189921
2211	21	-0.9058618847194211
2211	22	-0.555790735701116
2211	23	-5.3233637025692175
2211	24	-2.356126588252156
2211	25	-1.7858680083677996
2212	1	0.3736748386845079
2212	2	0.5687105910209702
2212	3	0.288992509801659
2212	4	0.8723339593161441
2212	5	0.7588307188454415
2212	6	0.2285789304300323
2212	7	0.2518220023380877
2212	8	0.0806246086388569
2212	9	0.07330197970937746
2212	10	-3.789211522039924
2212	11	-1.0690408295208371
2212	12	-0.18938183219704285
2212	13	0.003496501475424907
2212	14	0.792236420624377
2212	15	0.35085969171110776
2212	16	0.5419136316708872
2212	17	1.0866615264480401
2212	18	1.7717898583805223
2212	19	0.9847014285801947
2212	20	0.4644562724953474
2212	21	0.3196092480202424
2212	22	0.4547921982480361
2212	23	1.8407370635102787
2212	24	1.009687004342588
2212	25	0.9784559559134607
2213	1	-1.5382060646024023
2213	2	-4.02425685944749
2213	3	-1.8479809565892111
2213	4	-3.3505682123796703
2213	5	-1.2561777467066655
2213	6	-1.0924187547224449
2213	7	-0.8618917572909588
2213	8	-0.9138580180482272
2213	9	-0.8400225782902524
2213	10	-1.0108406906404603
2213	11	-0.8647021842483085
2213	12	-0.7393485170882883
2213	13	-1.1611268747526928
2213	14	-6.30580347707161
2213	15	-2.935568178632792
2213	16	-2.2463379646101416
2213	17	-0.8902557589666102
2213	18	-0.20630495942623384
2213	19	-0.8642035931063319
2213	20	-1.099158375138046
2213	21	-1.42007873850444
2213	22	-1.2502442708359425
2213	23	-5.849979705891556
2213	24	-3.554103367355477
2213	25	-2.7037468919758076
2214	1	-0.17471701189784383
2214	2	-0.048379393054002406
2214	3	-0.282686704558909
2214	4	0.06807566486763861
2214	5	-0.3226650633238819
2214	6	-0.3559527049949658
2214	7	-0.5015347243355706
2214	8	-0.5171317930400683
2214	9	-0.5422508092756289
2214	10	-0.07452713409727352
2214	11	-0.6111577626810387
2214	12	-0.5492278557752215
2214	13	-0.6290341219984867
2214	14	-0.1172023425972668
2214	15	-0.4978694996878801
2214	16	0.04556036654076934
2214	17	0.25202953444944853
2214	18	0.7746874425792356
2214	19	-0.1759040578646207
2214	20	-0.2292636718259331
2214	21	-0.4568810515579571
2214	22	0.026215927073693024
2214	23	1.4741872208158675
2214	24	0.5628140794935135
2214	25	0.3748310586339869
2215	1	-2.8697869273349674
2215	2	-4.715846997681307
2215	3	-3.0342975951298037
2215	4	-4.515379593878301
2215	5	-5.4086970549495454
2215	6	-3.1239586025790764
2215	7	-2.346185722630615
2215	8	-2.8480548458120083
2215	9	-2.573062335352006
2215	10	-4.085121441719366
2215	11	-3.009101730532798
2215	12	-2.5672735452975743
2215	13	-2.7270189926847017
2215	14	-1.8486107659587248
2215	15	-2.556207759208533
2215	16	-3.310710424064034
2215	17	-7.695939000598965
2215	18	-4.203373449831009
2215	19	-3.2259701470837925
2215	20	-2.896454462969796
2215	21	-2.858227639886408
2215	22	-2.6372586430550022
2215	23	-6.791605625813805
2215	24	-4.480450228940811
2215	25	-4.326334216862811
2216	1	-1.4249222477287127
2216	2	-0.9065887435322537
2216	3	-1.0979616295402472
2216	4	-3.634183832668704
2216	5	-1.7913781850105224
2216	6	-1.363291127294172
2216	7	-1.4295523944785558
2216	8	-1.1242661380977736
2216	9	-1.372150856115745
2216	10	-0.9707038719399463
2216	11	-1.5525182445218064
2216	12	-2.2680353197389493
2216	13	-1.6832949999113005
2216	14	-7.4306562133615905
2216	15	-3.952638584551238
2216	16	-2.4646844599439213
2216	17	-1.421487429781589
2216	18	-0.696762018924975
2216	19	-1.1386111350250196
2216	20	-1.6640905371961914
2216	21	-1.9035247009158525
2216	22	-1.3001185609870447
2216	23	-0.2384706162964001
2216	24	-1.012460299295693
2216	25	-0.9733428390096073
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91033.0
3	1.0
4	773.0
5	4498.0
6	5532.0
7	4812.0
8	4592.0
9	4322.0
10	4153.0
11	3934.0
12	3881.0
13	3851.0
14	3710.0
15	3795.0
16	4050.0
17	4269.0
18	4784.0
19	5609.0
20	7136.0
21	8714.0
22	10772.0
23	13033.0
24	15344.0
25	17894.0
26	19902.0
27	22833.0
28	25416.0
29	30557.0
30	35147.0
31	40882.0
32	51568.0
33	65571.0
34	83564.0
35	107818.0
36	147982.0
37	197810.0
38	428944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25727360036156	16.698747178663567	11.50311658475039	25.54086263622448
2	14.808713308907892	18.576307322322393	40.270723144953635	26.344256223816082
3	20.66871241508531	22.801559715154397	27.6011381995801	28.928589670180195
4	11.4853748820555	15.173240644413799	36.850834818560365	36.49054965497033
5	13.3075710786208	37.25571502017856	35.16951003551831	14.267203865682324
6	35.17788579776133	33.948482800049995	17.017582290576144	13.856049111612537
7	30.54768617943432	30.107889594594234	19.919459100597507	19.424965125373944
8	27.27176206539707	35.485035010146895	18.945730912414156	18.297472012041876
9	27.110069640681502	15.08312461737274	18.372223831319424	39.434581910626335
10	15.131312778314676	28.56111733135122	33.328136109905145	22.97943378042896
11	36.014815392203374	22.317565996632133	21.433131162720688	20.234487448443804
12	24.223705156125792	24.38027108079541	29.83407371768812	21.56195004539068
13	29.966154857150922	20.110680403615255	24.11961976792862	25.803544971305204
14	21.86417500747565	20.791310103382354	26.68682572161017	30.657689167531828
15	26.093325610180585	27.503517752723823	21.804422356108123	24.59873428098747
16	23.469653800666595	26.90987528222772	26.53814106010107	23.082329857004623
17	24.0761585020432	26.914596867026365	26.56784760773251	22.44139702319793
18	23.655240582098006	25.855478049701553	28.066399543610647	22.42288182458979
19	24.60286205445887	26.480455262086178	26.61217374474302	22.30450893871193
20	24.57850124517687	26.069958377627938	26.52273774948896	22.828802627706228
21	23.797030957153773	25.796978540934045	26.908135198902755	23.49785530300943
22	24.004750102152734	26.14948708574378	25.351351641970794	24.49441117013269
23	22.461690233033863	25.889492879016874	27.795253842736923	23.853563045212343
24	23.62228908658457	27.227050360872656	25.784231824135823	23.36642872840695
25	23.82301867417899	25.534392800212895	25.747155326052184	24.895433199555928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	202.5
3	298.0
4	298.0
5	298.0
6	545.5
7	793.0
8	793.0
9	793.0
10	1233.5
11	1674.0
12	1674.0
13	1674.0
14	2806.5
15	3939.0
16	3939.0
17	3939.0
18	7196.0
19	10453.0
20	10453.0
21	10453.0
22	16680.0
23	22907.0
24	22907.0
25	22907.0
26	33178.0
27	43449.0
28	43449.0
29	43449.0
30	57075.0
31	70701.0
32	70701.0
33	70701.0
34	86380.0
35	102059.0
36	102059.0
37	102059.0
38	118933.0
39	135807.0
40	135807.0
41	135807.0
42	154120.0
43	172433.0
44	172433.0
45	172433.0
46	201309.0
47	230185.0
48	230185.0
49	230185.0
50	223826.5
51	217468.0
52	217468.0
53	217468.0
54	194710.0
55	171952.0
56	171952.0
57	171952.0
58	150002.0
59	128052.0
60	128052.0
61	128052.0
62	107034.0
63	86016.0
64	86016.0
65	86016.0
66	67869.0
67	49722.0
68	49722.0
69	49722.0
70	37026.0
71	24330.0
72	24330.0
73	24330.0
74	17134.5
75	9939.0
76	9939.0
77	9939.0
78	7027.0
79	4115.0
80	4115.0
81	4115.0
82	2770.0
83	1425.0
84	1425.0
85	1425.0
86	944.5
87	464.0
88	464.0
89	464.0
90	305.0
91	146.0
92	146.0
93	146.0
94	87.5
95	29.0
96	29.0
97	29.0
98	26.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10762613823710804
2	0.046557374405939995
3	0.03506919111096778
4	0.03345681450816467
5	0.03500200875251766
6	0.01907978979983688
7	0.0200203428181387
8	0.023245096023744933
9	0.028149408190604415
10	0.03439736752646649
11	0.020423436968839477
12	0.01981879574278831
13	0.01592221895268078
14	0.021565537062491685
15	0.02149835470404156
16	0.021901448854742333
17	0.02546211385259922
18	0.019012607441386754
19	0.031172614320860256
20	0.02311073130684467
21	0.02667139630470156
22	0.03480046167716727
23	0.028149408190604415
24	0.03795803252432337
25	0.029694602434957397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1488486.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.58282303092997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.97491847603881	65.73809871304003
2	9.059931538108966	13.695504042344622
3	2.1353781251362602	4.84193720808879
4	0.7837357043325884	2.369478281743651
5	0.34128119056192874	1.2897497915013674
6	0.18629685622392703	0.844850538911501
7	0.11768078084449198	0.6226251942897624
8	0.08344814293611733	0.5045796975840243
9	0.050791455324376185	0.345506542133914
>10	0.2409854807104562	3.0953439737947943
>50	0.013907745490695064	0.7260259486737618
>100	0.008124175878010054	1.2784236407363305
>500	0.002256620777811481	1.291971287042849
>1k	0.0010831779733495109	1.939355537069231
>5k	9.026483111245924E-5	0.6343533308826325
>10k+	9.026483111245924E-5	0.7821962721627641
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11465	0.770245739630739	No Hit
CCCATGTACTCTGCGTTGATACCAC	9298	0.6246615688693075	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4765	0.32012393801486877	No Hit
GCGTTGATACCACTGCTTCCCATGT	3128	0.21014641723200622	No Hit
CATGTACTCTGCGTTGATACCACTG	3038	0.2041000049714945	No Hit
GTATCAACGCAGAGTACATGGGAAG	3036	0.20396564025459427	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2561	0.17205401999078257	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2371	0.1592893718852579	No Hit
TATCAACGCAGAGTACATGGGAAGC	2227	0.1496151122684392	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2127	0.14289687642342622	No Hit
GGTATCAACGCAGAGTACATGGGAA	1494	0.10037044352449401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.718235845012987E-5
2	0.0	0.0	0.0	0.0	6.718235845012987E-5
3	0.0	0.0	0.0	0.0	6.718235845012987E-5
4	0.0	0.0	0.0	0.0	6.718235845012987E-5
5	0.0	0.0	0.0	0.0	6.718235845012987E-5
6	0.0	0.0	0.0	0.0	6.718235845012987E-5
7	0.0	0.0	0.0	0.0	6.718235845012987E-5
8	0.0	0.0	0.0	0.0	6.718235845012987E-5
9	0.0	0.0	0.0	0.0	6.718235845012987E-5
10	0.0	0.0	0.0	0.0	6.718235845012987E-5
11	0.0	0.0	0.0	0.0	6.718235845012987E-5
12	0.0	0.0	0.0	0.0	3.359117922506493E-4
13	0.0	0.0	0.0	0.0	4.0309415070077917E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAC	45	6.7616336E-4	14.777908	19
AAGATCG	40	0.005275123	14.251563	5
TTACCCG	40	0.0052821855	14.248689	10
GCGTTTA	170	0.0	13.409179	13
GTAGGAC	470	0.0	12.937155	3
TGTAGGA	505	0.0	12.604919	2
GTCCTAG	250	0.0	12.553613	1
TAGGACC	315	0.0	12.065626	4
TGCACCG	80	2.8629522E-5	11.876303	5
GGCGAGA	240	0.0	11.875105	19
GTGTAGG	230	0.0	11.577772	1
GCACCGT	125	1.8335413E-8	11.398952	6
CGTTTAT	195	0.0	11.202968	14
TGCGTTT	205	0.0	11.119806	12
TAGGACT	165	8.0035534E-11	10.94133	4
TAGGACG	315	0.0	10.859064	4
ACCGTTC	70	0.0014924819	10.856145	8
TTAGGAC	230	0.0	10.739947	3
GGACCGT	80	3.7736766E-4	10.686517	6
CACCGTT	80	3.7736766E-4	10.686517	7
>>END_MODULE