FastQCFastQC Report
Thu 26 May 2016
SRR1513110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488486
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC136160.9147549926569681No Hit
CCCATGTACTCTGCGTTGATACCAC128130.8608075588215139No Hit
GAGTACATGGGAAGCAGTGGTATCA51880.34854207563927375No Hit
CATGTACTCTGCGTTGATACCACTG38000.25529296211049346No Hit
GCGTTGATACCACTGCTTCCCATGT33130.22257515354528026No Hit
GTATCAACGCAGAGTACATGGGAAG32330.21720056486926984No Hit
GCTTCCCATGTACTCTGCGTTGATA31890.21424454109746413No Hit
ACGCAGAGTACATGGGAAGCAGTGG31010.2083324935538527No Hit
TATCAACGCAGAGTACATGGGAAGC26760.1797799912125475No Hit
ACTCTGCGTTGATACCACTGCTTCC25310.17003854923727868No Hit
GTATCAACGCAGAGTACTTTTTTTT22450.15082439472054154No Hit
GGTATCAACGCAGAGTACATGGGAA18800.12630283388624414No Hit
TATCAACGCAGAGTACTTTTTTTTT17610.11830813323067868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2600.012.8158094
CTAGGAC1600.012.4954163
TATACCG550.003019631112.1167655
GTAGGAC4750.012.0256633
ACCGTGG1201.0224539E-811.8580118
TGTAGGA5800.011.3343022
CCTGAAC2200.011.2512823
GTTCACG600.005743672611.1171571
TTGGACA1201.2377131E-711.1070364
AGGCCGT600.00578275511.1070366
TAGGACG3000.011.1070364
TAGGACA2950.010.97256854
CCTACAC3500.010.8803623
GCCCTAA803.6657607E-410.7201161
TTCAGGG2350.010.54093552
GTATTAA1451.3755198E-810.514751
TCAAGAC1002.3422928E-510.4723483
TTAGGAC2950.010.3271243
TGCACCG1201.4818706E-610.3136765
GTGTAAG2500.010.2913111
Produced by FastQC (version 0.11.5)