FastQCFastQC Report
Thu 26 May 2016
SRR1513109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162079
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC117140.5417933387262908No Hit
CCCATGTACTCTGCGTTGATACCAC96510.4463759187337743No Hit
GAGTACATGGGAAGCAGTGGTATCA48370.2237198548249162No Hit
GCGTTGATACCACTGCTTCCCATGT32150.14869946935334No Hit
GTATCAACGCAGAGTACATGGGAAG31620.14624812506851045No Hit
CATGTACTCTGCGTTGATACCACTG31090.1437967807836809No Hit
ACGCAGAGTACATGGGAAGCAGTGG24720.11433439758676718No Hit
TATCAACGCAGAGTACATGGGAAGC24280.11229931931256906No Hit
GTCCTACAGTGGACATTTCTAAATT22900.10591657381622041No Hit
GCTTCCCATGTACTCTGCGTTGATA22840.10563906314246611No Hit
GTATCAACGCAGAGTACTTTTTTTT22690.10494528645808038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5700.013.502224
GCGTTTA2500.012.5377113
GTAGGAC13800.012.1865043
CGTTTAT2350.011.72126314
GTCCTAC16100.011.6396031
TGTAGGA15550.011.4265792
GGACGTG10600.011.2911746
AGGACCT10500.011.1293725
AGGACGT11300.011.0140665
TAGCACC2000.010.9267964
GGACCTG9850.010.8008086
TCCTACA18200.010.7547642
TCGTCAA803.7767168E-410.68604310
TAGGACG11900.010.6193924
TGCGTTT3250.010.52164212
CTGTAGG15100.010.5205231
GACGTGG6150.010.5026337
GGCGAGG3550.010.43543819
TAGGACA5700.010.3350324
GACGTGA5350.010.2976627
Produced by FastQC (version 0.11.5)