##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513109_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2162079
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.34962182232934	31.0	30.0	34.0	25.0	34.0
2	29.02108387343848	31.0	30.0	34.0	16.0	34.0
3	29.40139560117831	31.0	30.0	34.0	23.0	34.0
4	32.267641469160004	35.0	33.0	37.0	19.0	37.0
5	32.49660211305877	35.0	33.0	37.0	22.0	37.0
6	32.95493781679578	37.0	33.0	37.0	26.0	37.0
7	32.93224391893173	37.0	35.0	37.0	26.0	37.0
8	32.91649750078513	37.0	35.0	37.0	26.0	37.0
9	34.30874958778102	39.0	35.0	39.0	26.0	39.0
10	33.80859857572272	39.0	34.0	39.0	22.0	39.0
11	34.19835121658367	39.0	35.0	39.0	26.0	39.0
12	34.03766837381983	39.0	35.0	39.0	25.0	39.0
13	34.12694540763774	39.0	35.0	39.0	25.0	39.0
14	34.439630559290386	40.0	34.0	41.0	17.0	41.0
15	34.99048554655033	40.0	34.0	41.0	24.0	41.0
16	34.581745625391115	39.0	34.0	41.0	17.0	41.0
17	34.118326388628724	39.0	33.0	41.0	16.0	41.0
18	33.5222959013061	39.0	32.0	41.0	10.0	41.0
19	34.41958041311164	39.0	34.0	41.0	18.0	41.0
20	34.68580842790666	39.0	34.0	41.0	18.0	41.0
21	34.705939514698585	40.0	34.0	41.0	18.0	41.0
22	34.29695122148636	40.0	34.0	41.0	11.0	41.0
23	32.62346102986986	39.0	31.0	41.0	8.0	41.0
24	33.73095478934859	39.0	33.0	41.0	2.0	41.0
25	33.464551480311314	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6074503224857075
1101	2	0.9748544611956582
1101	3	0.7618562641585136
1101	4	0.7581420236173884
1101	5	0.9592437775387239
1101	6	0.5438216364519661
1101	7	0.6248609741787803
1101	8	0.5981741642220655
1101	9	0.5879720435059355
1101	10	1.0477822334801488
1101	11	0.671412103691928
1101	12	0.7793472554173135
1101	13	0.5955443832855067
1101	14	-0.2780210162378509
1101	15	0.28065554485787203
1101	16	1.0510311211255399
1101	17	1.668655573067582
1101	18	-4.7580750274131525
1101	19	-0.8089791689646688
1101	20	0.027556270962939777
1101	21	0.5185080673087867
1101	22	1.0225243129224282
1101	23	2.8283840809814293
1101	24	1.7018333583500294
1101	25	1.9861805507670667
1102	1	1.0496170045138271
1102	2	1.2692143607431206
1102	3	1.0079106459851168
1102	4	1.5724797799118804
1102	5	1.3863070200801744
1102	6	0.9911480605441909
1102	7	0.859481881915606
1102	8	0.8696119281403938
1102	9	1.1081678053373807
1102	10	1.3901887817839622
1102	11	1.0033634750147087
1102	12	1.0459919608926995
1102	13	0.961369754264652
1102	14	1.8688893968942608
1102	15	1.3594706191105885
1102	16	1.6462212219763401
1102	17	1.379052035153805
1102	18	1.9502979896790293
1102	19	1.5605522952778443
1102	20	1.4490347711842588
1102	21	1.3558336721234099
1102	22	1.7097286760308137
1102	23	3.20826489884805
1102	24	2.055687035370312
1102	25	2.4563739230388464
1103	1	0.7728358345152095
1103	2	1.0780794511106002
1103	3	0.8345346725364848
1103	4	1.199580981308145
1103	5	1.1337287920387311
1103	6	0.7536113034869913
1103	7	0.6630479151437711
1103	8	0.61325042020691
1103	9	0.815525220041863
1103	10	1.0842460485992405
1103	11	0.836341740656529
1103	12	0.8594485135829686
1103	13	0.7203978690203456
1103	14	1.5542829555796018
1103	15	1.1135058620372078
1103	16	1.2672272279696273
1103	17	1.8709432526130314
1103	18	1.8902288354590198
1103	19	1.385850710688885
1103	20	1.2501299623529505
1103	21	1.1845328710263345
1103	22	1.5450354179133043
1103	23	3.0295850694122777
1103	24	1.8253406460657615
1103	25	2.041262200686255
1104	1	0.7239869976434505
1104	2	1.1508121828263036
1104	3	0.8920274208954204
1104	4	1.4312542588712631
1104	5	1.229323452657468
1104	6	0.761084103785322
1104	7	0.6872445218893404
1104	8	0.7963009513240209
1104	9	0.8969250305352858
1104	10	1.256013178810207
1104	11	0.8868143347752735
1104	12	0.8979583190223863
1104	13	0.784022319983471
1104	14	1.6548705859956314
1104	15	1.1137417218023415
1104	16	1.4996477204336571
1104	17	1.8697231086446067
1104	18	2.4855795417171294
1104	19	1.6037738756719975
1104	20	1.0576343408778186
1104	21	1.145783856333999
1104	22	1.428049439613524
1104	23	2.838995678654328
1104	24	2.0229285124345964
1104	25	2.0981990271135658
1105	1	0.5581060987800477
1105	2	0.9551400891908415
1105	3	0.6683764970668058
1105	4	0.7677610382429947
1105	5	0.8404501127863142
1105	6	0.5673400883491269
1105	7	0.25952828929339233
1105	8	0.3606612285738606
1105	9	0.48573378774207754
1105	10	0.9917370108981771
1105	11	0.5698222958723562
1105	12	0.5108087504895522
1105	13	0.5774178204593454
1105	14	1.2883849388238175
1105	15	0.7999679679657135
1105	16	1.0921220681704398
1105	17	1.554617563358633
1105	18	1.5771925461923502
1105	19	1.1551641024996258
1105	20	0.8479728321457785
1105	21	0.6827785855785891
1105	22	1.2236286730686459
1105	23	2.5768406860455357
1105	24	1.6101046227791045
1105	25	1.7370965945239831
1106	1	-0.5086739420852204
1106	2	-0.08717437115786453
1106	3	-0.24428964861711577
1106	4	-2.747015527205736
1106	5	-0.5219117965604916
1106	6	-0.4983652882147851
1106	7	-0.5748283244946251
1106	8	-0.4708547103106895
1106	9	-0.44547038583530707
1106	10	-0.2801005743797731
1106	11	-0.28269974422573085
1106	12	-0.3347184917421444
1106	13	-0.3040459651947245
1106	14	0.32487378990298765
1106	15	-0.25258396818862394
1106	16	-1.3561269791675485
1106	17	-5.996583554665943
1106	18	-7.0387156729149645
1106	19	-3.0672092486834117
1106	20	-1.7046477989907416
1106	21	-1.219656953797923
1106	22	-1.490790073956866
1106	23	-5.371913681581429
1106	24	-2.3191738930575596
1106	25	-0.9614679001722166
1107	1	0.6480060917313111
1107	2	1.0260810175909114
1107	3	0.722856146230999
1107	4	1.1216775677426654
1107	5	1.193420641629281
1107	6	0.6203664226412968
1107	7	0.5644068650660685
1107	8	0.5524729345062411
1107	9	0.4990117123390121
1107	10	0.9374879510544005
1107	11	0.6418815470556751
1107	12	0.7486631398865526
1107	13	0.6505282720354089
1107	14	1.3661568544614227
1107	15	0.8270297145434427
1107	16	-5.751264038592769
1107	17	-1.0511064888785242
1107	18	0.783403864479645
1107	19	0.7406008106900757
1107	20	0.5836745232403899
1107	21	0.7193845516433512
1107	22	0.871627788257392
1107	23	2.727684267497942
1107	24	1.5932242270864165
1107	25	1.313559003682947
1108	1	0.3323035052292198
1108	2	0.6576887070535626
1108	3	0.45080440603663874
1108	4	0.9620962905584634
1108	5	0.6510259940484389
1108	6	0.36156900170709605
1108	7	0.32907766405406136
1108	8	0.3412363346346794
1108	9	0.3828086768541894
1108	10	0.830783685553719
1108	11	0.3909089737866154
1108	12	0.5337661040097004
1108	13	0.4465144920104933
1108	14	1.055940736270891
1108	15	0.5848846858638552
1108	16	0.8910777356277393
1108	17	1.0583728933162533
1108	18	0.7186348441187249
1108	19	0.7928585174254081
1108	20	0.5377425614677307
1108	21	0.6570174975979057
1108	22	1.0211976812380428
1108	23	2.7095999217975333
1108	24	1.4659269588410737
1108	25	1.6306051987201897
1109	1	0.25895160930297934
1109	2	0.5889753560285911
1109	3	0.45303111732936685
1109	4	0.45752831076460865
1109	5	0.266843331735366
1109	6	0.1487537027652195
1109	7	0.2710830106496047
1109	8	0.10238180007110742
1109	9	0.2532268773195341
1109	10	0.7128680579597457
1109	11	0.3898671164134484
1109	12	0.4636022578736245
1109	13	0.12082905952814826
1109	14	-0.9382140021671788
1109	15	-0.09744106542346742
1109	16	-0.2982415154933875
1109	17	0.6697367195447868
1109	18	1.5138389941291237
1109	19	0.819267020076488
1109	20	0.5692000242166415
1109	21	0.39366736135570335
1109	22	0.3114917795421732
1109	23	-4.228384724514861
1109	24	-1.4275167072739876
1109	25	-0.10458332742889098
1110	1	0.7107042870050222
1110	2	0.9909234724487526
1110	3	0.7138899851434637
1110	4	1.268664921035075
1110	5	0.9468605937469192
1110	6	0.6327629138163928
1110	7	0.5918499421397954
1110	8	0.5456016268239168
1110	9	0.588289402032423
1110	10	0.957246320715079
1110	11	0.6086416877808105
1110	12	0.6809925587482226
1110	13	0.47111786291018376
1110	14	1.3423726797815618
1110	15	0.8209710211200019
1110	16	1.3135489087061742
1110	17	1.5435695306050121
1110	18	2.1439277602349662
1110	19	1.3420728198336676
1110	20	1.0003374880073537
1110	21	0.8627388358380585
1110	22	1.0890787732870848
1110	23	2.4775763305755163
1110	24	1.6402613356563407
1110	25	1.7986212056345678
1111	1	0.47220072467632335
1111	2	0.720665361351088
1111	3	0.5052284241808209
1111	4	1.0741709944124125
1111	5	0.802384171926974
1111	6	0.37947907061560926
1111	7	0.2319643085759111
1111	8	0.08429155341586636
1111	9	0.11873840043771366
1111	10	0.47138734637490387
1111	11	0.22049478958096103
1111	12	0.4391226749354331
1111	13	0.23952272471711922
1111	14	-0.5441834333535454
1111	15	-0.03632675657650708
1111	16	0.3952197889169966
1111	17	0.9761004835726865
1111	18	1.7997627246617043
1111	19	0.8465683930031815
1111	20	0.5895364684075801
1111	21	0.5597862038600852
1111	22	0.7839634636875488
1111	23	0.770942075035066
1111	24	0.6755491893933012
1111	25	0.14438051947045238
1112	1	0.3940875260178416
1112	2	0.7967388475977515
1112	3	0.5567927403217574
1112	4	1.0727193949840341
1112	5	0.8028290059801506
1112	6	0.16455652342634153
1112	7	0.18479258043204538
1112	8	0.24270746630769935
1112	9	0.1864452783516981
1112	10	0.5840096693847556
1112	11	0.15416172868377487
1112	12	0.20821897718670357
1112	13	0.2574197967261753
1112	14	0.5254666491652884
1112	15	0.36683575087763387
1112	16	0.7350769878655967
1112	17	0.8061294765853191
1112	18	1.0419766459509887
1112	19	0.35672086178134066
1112	20	0.3692845550704078
1112	21	0.35486547498296517
1112	22	0.4123170676144241
1112	23	2.0065977019655463
1112	24	0.9768121622370671
1112	25	1.0429941962619935
1113	1	0.3724415246090125
1113	2	0.8734140989847532
1113	3	0.6048774592350803
1113	4	0.6364538861932871
1113	5	0.6950613330652118
1113	6	0.3518120213784144
1113	7	0.37908057930813754
1113	8	0.2589544817332836
1113	9	0.46436504064926254
1113	10	0.6805355820884529
1113	11	0.35774318516265424
1113	12	0.4187063958556152
1113	13	0.30315985629621167
1113	14	1.037399038420098
1113	15	0.5678925052231207
1113	16	0.9446024823842194
1113	17	1.3955838271215697
1113	18	2.0254778660404114
1113	19	1.0558943061755244
1113	20	0.6995941433255126
1113	21	0.7040391819737479
1113	22	0.9070495440513042
1113	23	0.7744455718804417
1113	24	0.7369830207112926
1113	25	1.2838248155501049
1114	1	-0.3762755155180564
1114	2	-3.2314793942192104
1114	3	-1.1070724413099207
1114	4	0.020491663087589984
1114	5	0.29341998949124815
1114	6	0.1020028008405589
1114	7	0.010575963789570153
1114	8	0.007702571537912206
1114	9	0.15688680583303238
1114	10	0.27395413878420527
1114	11	-0.04355549160892025
1114	12	0.08084829224188184
1114	13	0.11582850886184559
1114	14	0.7624659872867241
1114	15	0.10814953253338899
1114	16	0.49043244666749075
1114	17	0.8046205957015999
1114	18	0.9287441960901646
1114	19	0.3662667230504155
1114	20	0.21352632570811636
1114	21	-0.015603356212558595
1114	22	0.22607425767404266
1114	23	1.8458140560095728
1114	24	0.7954217148834886
1114	25	0.7288137497011036
1115	1	0.057889866168064685
1115	2	0.3304036744268899
1115	3	0.14196366695609797
1115	4	0.4055406174073113
1115	5	0.358832533902806
1115	6	-0.08112959405053743
1115	7	-0.13606579739038693
1115	8	-0.13545817421352524
1115	9	-0.18838512231310744
1115	10	0.11096826919316527
1115	11	0.06898551208907122
1115	12	-0.08832049104724149
1115	13	-0.13431827906196503
1115	14	0.5774028387779921
1115	15	-0.10499200416577992
1115	16	0.4485217587558097
1115	17	0.9318583690022706
1115	18	1.231440904413958
1115	19	0.46365949439391585
1115	20	-0.003793586300417928
1115	21	0.14395042725790574
1115	22	0.3280329306987042
1115	23	1.967049156800826
1115	24	0.7429896625288208
1115	25	0.5619968559899391
1116	1	-0.004654978756164496
1116	2	-0.07081723144507635
1116	3	0.06674048384614295
1116	4	0.5867923370247041
1116	5	0.041487446328943633
1116	6	-0.06351044666905636
1116	7	-0.20768168027117895
1116	8	-0.16935996285538835
1116	9	-0.06679348641223015
1116	10	0.37304320524886947
1116	11	-0.07358830333056687
1116	12	0.009433051739236475
1116	13	-0.07148031242748942
1116	14	0.473515619304159
1116	15	-0.1924297158023478
1116	16	0.2586404820399295
1116	17	0.4915774217336093
1116	18	1.0326164183809965
1116	19	0.13540723708700142
1116	20	-0.0855859326467936
1116	21	-0.20172376466428688
1116	22	0.16963631244807686
1116	23	1.5711438542950233
1116	24	0.45345592086315634
1116	25	0.7118260331691246
1201	1	1.2065427598742602
1201	2	1.5051214955063656
1201	3	1.2716121372603588
1201	4	1.8534677508456738
1201	5	1.6877182540621476
1201	6	1.1532898187124374
1201	7	1.0828911125494045
1201	8	1.2063551234025525
1201	9	1.3466962624088836
1201	10	1.7440148785360066
1201	11	1.3178905872540412
1201	12	1.5543651628239985
1201	13	1.5077807985685325
1201	14	2.304537990279158
1201	15	1.7285781098315667
1201	16	2.0979652324199307
1201	17	2.3821543579430653
1201	18	2.9692898346793015
1201	19	2.0934799781152194
1201	20	1.9089711378033485
1201	21	1.6264741962257077
1201	22	1.9590928200367443
1201	23	3.6051751440424624
1201	24	2.400138690478201
1201	25	2.604177465183149
1202	1	1.0385007139845222
1202	2	1.4569856303863986
1202	3	1.2724448040573364
1202	4	1.6701891702924527
1202	5	1.4025794548063857
1202	6	1.1731582528379647
1202	7	1.0294951872018672
1202	8	0.934368391038447
1202	9	1.0950785666326865
1202	10	1.5601696560907357
1202	11	1.1757211585766711
1202	12	1.2503747597682633
1202	13	1.143555360003333
1202	14	2.0832851653000546
1202	15	1.4483466691470142
1202	16	1.7459337976514107
1202	17	2.1941053130530577
1202	18	2.8198281592176926
1202	19	2.0204274951456824
1202	20	1.6656539693893393
1202	21	1.6188883098345528
1202	22	1.879889923814659
1202	23	3.437837125643995
1202	24	2.287304035422679
1202	25	2.420029955110415
1203	1	0.8704302550740053
1203	2	1.1751993246667247
1203	3	0.9265157078674306
1203	4	1.542440473610739
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2211	14	-0.09224889300578809
2211	15	-0.26558532169769933
2211	16	-0.33672685862064355
2211	17	0.16256981483146404
2211	18	-5.366178689336838
2211	19	-1.7441520100534191
2211	20	-0.9767871892772035
2211	21	-0.8609881511247437
2211	22	-0.46407783210170805
2211	23	-4.796573189453987
2211	24	-2.178950088216652
2211	25	-1.5411353916334782
2212	1	0.44934128098099535
2212	2	0.7076528716858128
2212	3	0.5139153722910166
2212	4	1.0445011381574503
2212	5	0.9294681616796865
2212	6	0.45065106605946426
2212	7	0.46492306237072967
2212	8	0.32938534552675236
2212	9	0.20479873632414325
2212	10	-3.281107346846241
2212	11	-0.7231465727446462
2212	12	-0.03407919391353431
2212	13	0.11081094330427987
2212	14	1.0175622728398608
2212	15	0.563870338290009
2212	16	0.8031287832341505
2212	17	1.3275297209432608
2212	18	1.8484903346086838
2212	19	1.0391939155814853
2212	20	0.7374164232022764
2212	21	0.5044016561626705
2212	22	0.7775588648872187
2212	23	2.2205410076104926
2212	24	1.313343675068225
2212	25	1.1216198543193556
2213	1	-1.4565845347383792
2213	2	-3.913869063122984
2213	3	-1.7578456919296528
2213	4	-3.241264602356676
2213	5	-1.276024536647256
2213	6	-0.952842833330827
2213	7	-0.8761383926017459
2213	8	-0.7010999307838333
2213	9	-0.6799928666587505
2213	10	-0.7551127712654022
2213	11	-0.8087430709746997
2213	12	-0.651144702982613
2213	13	-1.022923297393831
2213	14	-5.929080115285725
2213	15	-2.6351531884016595
2213	16	-1.9234178989637414
2213	17	-0.6768905679551267
2213	18	0.013986066060667213
2213	19	-0.5929759468683216
2213	20	-0.6928931281965021
2213	21	-1.0934777290472582
2213	22	-0.9827223626753607
2213	23	-5.972305065694314
2213	24	-3.194362442873622
2213	25	-2.2409172938457544
2214	1	-0.06535280968229173
2214	2	-0.013670863147908108
2214	3	-0.11041842688941017
2214	4	0.2906346324961362
2214	5	-0.07065917769840269
2214	6	-0.16162463478169542
2214	7	-0.29399434796294344
2214	8	-0.3157330792549047
2214	9	-0.36507429556429116
2214	10	0.24420773831742792
2214	11	-0.2526358864005189
2214	12	-0.22419100561521788
2214	13	-0.25279262334066743
2214	14	0.26161331476324534
2214	15	-0.047228061282481804
2214	16	0.38537826190707136
2214	17	0.6374359685860966
2214	18	0.9907244720784334
2214	19	0.10011110853105265
2214	20	-0.0988605832874967
2214	21	-0.20351986111793963
2214	22	0.31127826500787137
2214	23	1.7470350739818628
2214	24	0.6905314806596365
2214	25	0.5910701631509454
2215	1	-2.6369879664416125
2215	2	-4.739359433244704
2215	3	-2.65298411235203
2215	4	-4.166779018931621
2215	5	-5.19955165104059
2215	6	-2.5238064989786118
2215	7	-1.8245829582647026
2215	8	-2.1872343262495413
2215	9	-1.8957891270318044
2215	10	-3.425651992805861
2215	11	-2.3305619188752367
2215	12	-1.9353700682788997
2215	13	-1.9653167102795024
2215	14	-1.1589067548826506
2215	15	-1.6632344428195935
2215	16	-2.543548169413718
2215	17	-6.890395798462169
2215	18	-3.161166362631107
2215	19	-2.2851246991963095
2215	20	-2.0337733897702677
2215	21	-1.8872024116824875
2215	22	-1.6935674744671587
2215	23	-6.420479794647214
2215	24	-3.8335822080898616
2215	25	-3.530439172890482
2216	1	-0.9791726216783943
2216	2	-0.4736642912264415
2216	3	-0.666402982873965
2216	4	-3.0551247178224834
2216	5	-1.2695184799011727
2216	6	-1.011573585034597
2216	7	-1.0084384924982714
2216	8	-0.8950068819329644
2216	9	-0.9562890448337527
2216	10	-0.5623723980499449
2216	11	-1.1140328891349043
2216	12	-1.9543138770754709
2216	13	-1.239590102539367
2216	14	-6.934634822214374
2216	15	-3.416329468377807
2216	16	-1.7446577663052452
2216	17	-0.8787063135136322
2216	18	-0.08668924211340112
2216	19	-0.5743616708049544
2216	20	-1.1368251653250283
2216	21	-1.3414774018110407
2216	22	-0.766135449183956
2216	23	0.2583422995747995
2216	24	-0.5532339956286378
2216	25	-0.47638004867616246
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99985.0
3	5.0
4	857.0
5	5084.0
6	6330.0
7	5548.0
8	5169.0
9	4828.0
10	4586.0
11	4453.0
12	4455.0
13	4401.0
14	4634.0
15	4599.0
16	4680.0
17	4904.0
18	5614.0
19	6567.0
20	8335.0
21	10364.0
22	12802.0
23	15803.0
24	18332.0
25	21511.0
26	24657.0
27	28291.0
28	31785.0
29	38217.0
30	45011.0
31	51441.0
32	66293.0
33	87691.0
34	114951.0
35	150944.0
36	213023.0
37	298285.0
38	747644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91743644335618	17.65657579682248	11.892060090507124	25.533927669314217
2	14.0033845457587	19.477102950996276	41.61538251521634	24.904129988028682
3	19.815045815188324	23.793545712471982	28.476577898775364	27.91483057356433
4	10.831469553220215	15.96462997217516	38.67125509744246	34.53264537716216
5	12.523307839299678	37.82352206321225	36.153315843778486	13.49985425370959
6	33.42819896246115	35.66236779799172	17.700196238620983	13.209237000926144
7	29.132419795589726	30.753277730308522	21.174547205746766	18.93975526835499
8	25.969462767133706	36.54086543132315	19.896667126211742	17.593004675331407
9	26.897783979398938	15.055041958100194	19.264546538838424	38.78262752366244
10	14.2202038616184	29.177751969906584	34.74924465707663	21.85279951139839
11	35.07246604765934	22.48226225265806	23.093281792052583	19.351989907630017
12	23.547946270126037	24.740242076805075	31.24825363105955	20.463558022009337
13	29.461498998715374	21.2082214048701	24.894725179868097	24.43555441654643
14	20.79469138640991	21.668064538662918	27.892195618191735	29.64504845673544
15	24.51430450996511	29.428508908484535	22.62874891688576	23.428437664664592
16	22.280383845122202	27.727133730970703	27.872165122055364	22.12031730185173
17	22.3324075911782	27.905319848309677	27.913878683826898	21.848393876685222
18	21.98358852325391	26.82306821893195	29.739672048029796	21.453671209784343
19	23.60245458495019	27.092784440044916	27.462785869711563	21.84197510529333
20	23.68575789160559	26.899627076443082	27.651210764032978	21.76340426791835
21	23.014820661391795	26.619821397011812	27.325507693475004	23.039850248121393
22	23.032311976169257	27.39841257943349	26.608403871477606	22.960871572919643
23	21.14164366893444	27.079476924002233	29.015218985447234	22.763660421616095
24	22.910565682411814	27.618100171432935	27.199165276617798	22.272168869537452
25	22.809137701694524	26.5642472962813	27.186397547857265	23.44021745416691
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	171.0
2	305.0
3	439.0
4	439.0
5	439.0
6	908.5
7	1378.0
8	1378.0
9	1378.0
10	2207.5
11	3037.0
12	3037.0
13	3037.0
14	5355.5
15	7674.0
16	7674.0
17	7674.0
18	14097.0
19	20520.0
20	20520.0
21	20520.0
22	33269.5
23	46019.0
24	46019.0
25	46019.0
26	65937.0
27	85855.0
28	85855.0
29	85855.0
30	112033.0
31	138211.0
32	138211.0
33	138211.0
34	161832.5
35	185454.0
36	185454.0
37	185454.0
38	207468.0
39	229482.0
40	229482.0
41	229482.0
42	248669.0
43	267856.0
44	267856.0
45	267856.0
46	293838.0
47	319820.0
48	319820.0
49	319820.0
50	304776.5
51	289733.0
52	289733.0
53	289733.0
54	253588.5
55	217444.0
56	217444.0
57	217444.0
58	186437.5
59	155431.0
60	155431.0
61	155431.0
62	127086.5
63	98742.0
64	98742.0
65	98742.0
66	76100.5
67	53459.0
68	53459.0
69	53459.0
70	39169.5
71	24880.0
72	24880.0
73	24880.0
74	17612.5
75	10345.0
76	10345.0
77	10345.0
78	7174.0
79	4003.0
80	4003.0
81	4003.0
82	2732.5
83	1462.0
84	1462.0
85	1462.0
86	977.0
87	492.0
88	492.0
89	492.0
90	312.5
91	133.0
92	133.0
93	133.0
94	78.5
95	24.0
96	24.0
97	24.0
98	19.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11424189402884909
2	0.04995192127577207
3	0.03640015004077094
4	0.0324224970502928
5	0.03649265359868904
6	0.01965700605759549
7	0.020628293415735504
8	0.02409717683766412
9	0.029462383196913713
10	0.03741768917787
11	0.020628293415735504
12	0.020859552310530743
13	0.016465633309421163
14	0.02127581832116218
15	0.022570868132015528
16	0.022108350342425045
17	0.026363514006657478
18	0.0194719989417593
19	0.034041309313859486
20	0.02358840726911459
21	0.029462383196913713
22	0.03871273898872335
23	0.029323627860036564
24	0.040886572599798617
25	0.03256125238716994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2162079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.53942397171554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14302763636668	62.61369207586262
2	10.278001338842023	15.116765960779269
3	2.522791565423314	5.565739155657989
4	0.868619902223673	2.555112290395871
5	0.40143909318045834	1.4760799836109373
6	0.19841014616334604	0.8754580715397814
7	0.12875479168403917	0.6627987249829728
8	0.08142497230230088	0.4790356448019278
9	0.05933528309332856	0.3927134285893848
>10	0.2859667936396422	3.6727495413480082
>50	0.018311429713143357	0.9362786408759513
>100	0.010408268365425535	1.520394489853307
>500	0.002041471419703391	1.0899807854030126
>1k	0.0013397156191801466	2.040858009977672
>5k	6.379598186572126E-5	0.4527785718556933
>10k+	6.379598186572126E-5	0.5495646244656088
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11714	0.5417933387262908	No Hit
CCCATGTACTCTGCGTTGATACCAC	9651	0.4463759187337743	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4837	0.2237198548249162	No Hit
GCGTTGATACCACTGCTTCCCATGT	3215	0.14869946935334	No Hit
GTATCAACGCAGAGTACATGGGAAG	3162	0.14624812506851045	No Hit
CATGTACTCTGCGTTGATACCACTG	3109	0.1437967807836809	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2472	0.11433439758676718	No Hit
TATCAACGCAGAGTACATGGGAAGC	2428	0.11229931931256906	No Hit
GTCCTACAGTGGACATTTCTAAATT	2290	0.10591657381622041	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2284	0.10563906314246611	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2269	0.10494528645808038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.250355791809643E-5	0.0
10	0.0	0.0	0.0	9.250355791809643E-5	0.0
11	0.0	0.0	0.0	1.3875533687714462E-4	0.0
12	0.0	0.0	0.0	1.8500711583619285E-4	0.0
13	0.0	0.0	0.0	1.8500711583619285E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	570	0.0	13.50222	4
GCGTTTA	250	0.0	12.53771	13
GTAGGAC	1380	0.0	12.186504	3
CGTTTAT	235	0.0	11.721263	14
GTCCTAC	1610	0.0	11.639603	1
TGTAGGA	1555	0.0	11.426579	2
GGACGTG	1060	0.0	11.291174	6
AGGACCT	1050	0.0	11.129372	5
AGGACGT	1130	0.0	11.014066	5
TAGCACC	200	0.0	10.926796	4
GGACCTG	985	0.0	10.800808	6
TCCTACA	1820	0.0	10.754764	2
TCGTCAA	80	3.7767168E-4	10.686043	10
TAGGACG	1190	0.0	10.619392	4
TGCGTTT	325	0.0	10.521642	12
CTGTAGG	1510	0.0	10.520523	1
GACGTGG	615	0.0	10.502633	7
GGCGAGG	355	0.0	10.435438	19
TAGGACA	570	0.0	10.335032	4
GACGTGA	535	0.0	10.297662	7
>>END_MODULE