FastQCFastQC Report
Thu 26 May 2016
SRR1513109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162079
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC136850.6329555950545748No Hit
CCCATGTACTCTGCGTTGATACCAC125860.5821248899785808No Hit
GAGTACATGGGAAGCAGTGGTATCA50110.2317676643637906No Hit
CATGTACTCTGCGTTGATACCACTG36390.16831022363197645No Hit
GTATCAACGCAGAGTACTTTTTTTT35370.16359254217815353No Hit
GCGTTGATACCACTGCTTCCCATGT35090.16229749236730018No Hit
GTATCAACGCAGAGTACATGGGAAG34340.15882860894537157No Hit
ACGCAGAGTACATGGGAAGCAGTGG32220.14902323180605334No Hit
TATCAACGCAGAGTACATGGGAAGC30020.13884784043506274No Hit
GCTTCCCATGTACTCTGCGTTGATA28320.13098503801202455No Hit
ACTCTGCGTTGATACCACTGCTTCC26790.12390851583129016No Hit
TATCAACGCAGAGTACTTTTTTTTT24280.11229931931256906No Hit
GTCCTACAGTGGACATTTCTAAATT24240.11211431219673286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTG550.003023801112.1149425
AAGGCGT1552.3646862E-1111.6683096
TAGGACC6850.011.3948684
GTCCTAC14900.011.1910671
AGGACGT10100.011.0268265
TAGGACT3050.010.9233084
GTAGGAC13700.010.8390213
GGACGTG10100.010.8383336
TCCAACG1158.9274727E-710.72909718
AATCCCG1951.8189894E-1210.70347619
TTAGGAC6900.010.6225223
GGCGAGG4750.010.58568619
TGTAGGA15350.010.5501682
GTTCTAG2800.010.54929351
TGGCGAG11550.010.51830218
TCCTACA17200.010.4677212
TAGGACG10850.010.1768714
CTGTAGG15350.010.1180991
GTCCTAG2550.010.0888881
GACGTGA5650.010.0741737
Produced by FastQC (version 0.11.5)