FastQCFastQC Report
Thu 26 May 2016
SRR1513108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865804
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64280.34451635863145325No Hit
CCCATGTACTCTGCGTTGATACCAC52490.28132644157692877No Hit
GAGTACATGGGAAGCAGTGGTATCA26090.13983247972455842No Hit
GTATCAACGCAGAGTACATGGGAAG18840.10097523641282792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC11000.012.0173341
TTAGGCC951.0375461E-612.0024744
GTAGGAC11250.011.316373
TGTAGGA12300.011.2775362
TAGGACC5950.011.1787754
GCGTTTA1201.2790406E-711.0811613
CTAATCC1053.4638324E-610.8579243
AGGACGT8350.010.8100315
TCCTACA12150.010.6347962
CTGTAGG12100.010.6104651
GGCGAGG4050.010.55546619
GCCGGTT909.540625E-510.55291911
GGACGTG8350.010.4666976
TAGGACA4450.010.4628314
TAGGACG8450.010.3453264
CGTTTAT1201.5276783E-610.28964814
GATATAC4200.010.1894141
TGCACCG750.002644648810.1348785
ATTATAC2250.010.1340643
GACGTGG4800.010.0931237
Produced by FastQC (version 0.11.5)