##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1865804 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.68727583390324 31.0 28.0 34.0 20.0 34.0 2 28.39477672895974 31.0 28.0 34.0 16.0 34.0 3 28.77717434414333 31.0 29.0 34.0 16.0 34.0 4 31.58946920469674 35.0 32.0 37.0 16.0 37.0 5 31.795597501130878 35.0 32.0 37.0 17.0 37.0 6 32.273358294869126 35.0 33.0 37.0 22.0 37.0 7 32.24346126388409 35.0 33.0 37.0 22.0 37.0 8 32.243666537321175 35.0 33.0 37.0 22.0 37.0 9 33.53232976239734 38.0 34.0 39.0 19.0 39.0 10 32.980864549545394 38.0 33.0 39.0 17.0 39.0 11 33.41781076683296 38.0 33.0 39.0 18.0 39.0 12 33.33534551324791 38.0 33.0 39.0 17.0 39.0 13 33.373257855594694 38.0 34.0 39.0 17.0 39.0 14 33.722747941370045 39.0 33.0 41.0 10.0 41.0 15 34.17376744824215 39.0 33.0 41.0 17.0 41.0 16 33.70036509729854 39.0 33.0 41.0 10.0 41.0 17 33.18790558922588 38.0 32.0 41.0 10.0 41.0 18 32.527860911435496 38.0 31.0 41.0 10.0 41.0 19 33.47267612246517 39.0 32.0 41.0 10.0 41.0 20 33.77535153746052 39.0 33.0 41.0 10.0 41.0 21 33.81256391346572 39.0 34.0 41.0 2.0 41.0 22 33.36862500026798 39.0 33.0 41.0 2.0 41.0 23 31.541874709240627 38.0 30.0 40.0 2.0 41.0 24 32.64089100462857 38.0 32.0 41.0 2.0 41.0 25 32.369017324434935 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0819800921309763 1101 2 1.5645575843491208 1101 3 1.2444489119745796 1101 4 1.2644272139985624 1101 5 1.495533090427525 1101 6 1.1375277973173539 1101 7 1.1413508359922773 1101 8 1.118041595391226 1101 9 1.3436834882101678 1101 10 1.6749670783678638 1101 11 1.374916643966209 1101 12 1.3684680547324746 1101 13 1.3312669328513849 1101 14 0.3747291540464204 1101 15 0.99653726104372 1101 16 1.700507676211494 1101 17 2.273450557875634 1101 18 -3.8322258673876632 1101 19 -0.2780462564461956 1101 20 0.7316182542951779 1101 21 1.1103689328620447 1101 22 1.6660117459539379 1101 23 3.6049767400164967 1101 24 2.611143430173378 1101 25 2.7638590463747974 1102 1 1.0955093558462998 1102 2 1.4438569098481473 1102 3 1.2074569776743012 1102 4 1.6781206429250766 1102 5 1.5154715850371439 1102 6 1.1019520607713815 1102 7 1.065847152736822 1102 8 0.9728104456080402 1102 9 1.2315035267776793 1102 10 1.6447811225725388 1102 11 1.2583374192849277 1102 12 1.3596329870090713 1102 13 1.22589194879437 1102 14 2.1499018038299056 1102 15 1.60779368053619 1102 16 1.9220243913695398 1102 17 1.4929524999667052 1102 18 2.0290391117618114 1102 19 1.6943410909591492 1102 20 1.576850144914168 1102 21 1.6571227805530029 1102 22 1.9963054833484648 1102 23 3.698505659343276 1102 24 2.6131611065158253 1102 25 2.881205036120434 1103 1 1.3678005625366403 1103 2 1.6086284038405552 1103 3 1.4627296454213088 1103 4 1.9690259204675904 1103 5 1.812196386425665 1103 6 1.3912422471718742 1103 7 1.277767632718401 1103 8 1.1607227191371976 1103 9 1.426682306193264 1103 10 1.7505851394572716 1103 11 1.484760218088823 1103 12 1.6075914042799297 1103 13 1.4682118905058488 1103 14 2.3247381783063688 1103 15 1.7927914277137447 1103 16 2.0360012589152134 1103 17 2.5068594156702204 1103 18 2.465869208025339 1103 19 2.017762941691906 1103 20 1.8802572830914244 1103 21 1.8944707651896806 1103 22 2.2009730987549645 1103 23 3.7259757497651123 1103 24 2.6449050398435148 1103 25 2.8072856787385376 1104 1 0.9603691785339592 1104 2 1.3697137540516273 1104 3 1.0733785992945748 1104 4 1.7236771346089164 1104 5 1.533899777429962 1104 6 0.9741458083988448 1104 7 0.9073413132417585 1104 8 0.9624353840655004 1104 9 1.1762752170612174 1104 10 1.5692534280569674 1104 11 1.078251910700395 1104 12 1.1488596635724235 1104 13 1.0498488183880426 1104 14 2.041874362468036 1104 15 1.2839680518046706 1104 16 1.6177024217689322 1104 17 2.1482540371957555 1104 18 2.878598973507472 1104 19 2.0548510098452084 1104 20 1.4237155534443815 1104 21 1.536283716967283 1104 22 1.83780523069359 1104 23 3.3838628005970577 1104 24 2.3901272627348504 1104 25 2.3975200560908903 1105 1 1.0072963710091045 1105 2 1.4977464412028425 1105 3 1.181419448219149 1105 4 1.3577142331235201 1105 5 1.396914175699461 1105 6 1.0239676764434975 1105 7 0.8696424326064403 1105 8 0.9689998533123898 1105 9 1.302886854348003 1105 10 1.7543624478179751 1105 11 1.261245879500386 1105 12 1.3144395125045065 1105 13 1.284193360389878 1105 14 2.187479898453695 1105 15 1.4494898169508943 1105 16 1.8265967518400572 1105 17 2.308856205824007 1105 18 2.0861363461675353 1105 19 1.8132264664413498 1105 20 1.625653348714053 1105 21 1.411565310666191 1105 22 2.1148729008077325 1105 23 3.4769310515490517 1105 24 2.6032046268325644 1105 25 2.470055188018698 1106 1 -0.29004807074344896 1106 2 0.17656940964446122 1106 3 -0.05881920070573088 1106 4 -2.5873740874507654 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1107 17 -0.6088684286815251 1107 18 1.092197770640503 1107 19 1.0491307881682062 1107 20 0.9879038468260717 1107 21 1.2096997474046915 1107 22 1.2673283000362332 1107 23 3.1423730336017357 1107 24 2.280617603733411 1107 25 1.8387309489453116 1108 1 0.5972761019379647 1108 2 0.8444906762451936 1108 3 0.6251941034736959 1108 4 1.226753823715267 1108 5 1.078135446911677 1108 6 0.7328840958441063 1108 7 0.6452805243455231 1108 8 0.7253335488528876 1108 9 0.8706441900808315 1108 10 1.4004945631389205 1108 11 0.9833404124555329 1108 12 0.9735777708173714 1108 13 0.852128789958229 1108 14 1.3967134826336505 1108 15 1.1417463675555908 1108 16 1.3854771062011295 1108 17 1.4687740916965737 1108 18 1.2272769562323234 1108 19 1.3281934059569096 1108 20 1.013847367210488 1108 21 1.1544235506715097 1108 22 1.5302244648888177 1108 23 3.213822421574285 1108 24 1.9875980647597018 1108 25 2.224932167314769 1109 1 0.40947506110684984 1109 2 0.8316139755945748 1109 3 0.7027300798308893 1109 4 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1.7074042448970204 1110 17 1.9824715156563357 1110 18 2.5879528572410564 1110 19 1.77142806720844 1110 20 1.2927548498045525 1110 21 1.2601833096151296 1110 22 1.5950949347633383 1110 23 2.9246434584672016 1110 24 2.028989413501101 1110 25 2.2952327977679445 1111 1 0.19338264962525642 1111 2 0.5620808127722086 1111 3 0.30637206922955684 1111 4 0.8612666515028629 1111 5 0.47979414944834176 1111 6 0.018880894646017055 1111 7 0.04476050778894347 1111 8 -0.28735561947688737 1111 9 -0.238143429016759 1111 10 0.14427823417857155 1111 11 -0.22139635021468962 1111 12 0.03073845969032618 1111 13 -0.08859074764936992 1111 14 -0.9650863524482958 1111 15 -0.34533645486212095 1111 16 0.2029359430818829 1111 17 0.7425296437542599 1111 18 1.4390016842891882 1111 19 0.520892640376978 1111 20 0.21418750260394148 1111 21 0.21613114564675584 1111 22 0.4375283396858336 1111 23 0.37420911749349983 1111 24 0.4702525199762846 1111 25 0.06293321278901232 1112 1 0.459161761136226 1112 2 0.9073823408218189 1112 3 0.5842966146554041 1112 4 1.2934167021864802 1112 5 1.08689967659301 1112 6 0.40222144031037743 1112 7 0.2510721962372884 1112 8 0.21448363454447872 1112 9 0.32378769563337073 1112 10 0.6510677998427923 1112 11 0.28753773302569385 1112 12 0.38281892124443573 1112 13 0.3025101785103885 1112 14 0.6801229717584789 1112 15 0.4671038077456444 1112 16 0.8752380928388135 1112 17 0.9978841966861935 1112 18 1.1480977928748857 1112 19 0.46033328355029823 1112 20 0.3515033799039955 1112 21 0.4624010113439425 1112 22 0.4519446713846591 1112 23 2.1690344124756464 1112 24 1.0894848473538516 1112 25 1.1431380605277184 1113 1 0.20962665064372743 1113 2 0.7767445923745058 1113 3 0.33811992046090467 1113 4 0.6078143402842322 1113 5 0.7075766396930909 1113 6 0.23425137816727926 1113 7 0.11967876503780417 1113 8 0.05694829266811041 1113 9 0.20537923252256718 1113 10 0.5358494636983053 1113 11 0.2092241194349782 1113 12 0.3017663345692867 1113 13 0.16651397266986834 1113 14 0.8471974264429747 1113 15 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3 1.4697711195992014 2201 4 -1.094302716934056 2201 5 1.1348024508013985 2201 6 1.0590972349702596 2201 7 1.203308910200306 2201 8 1.1249753430294192 2201 9 1.4815433225016648 2201 10 2.016054451247477 2201 11 1.6146019888858874 2201 12 1.7612463146923787 2201 13 1.6231095803174824 2201 14 2.6901046184966546 2201 15 1.946466532786566 2201 16 2.5048661412893978 2201 17 2.90675044528286 2201 18 3.3291020823475037 2201 19 2.5999818663504755 2201 20 2.2311586919517126 2201 21 2.04927656929663 2201 22 2.5108129066310383 2201 23 3.8620883665003447 2201 24 2.9427948593921087 2201 25 3.1877653988566976 2202 1 1.300681103344786 2202 2 1.6434434844222103 2202 3 1.441525160089384 2202 4 2.0099256489351127 2202 5 1.7405994623633738 2202 6 1.2884296172959964 2202 7 1.074884119912987 2202 8 1.0717706024632605 2202 9 1.179709531754348 2202 10 0.7542870242915853 2202 11 1.1476572558657665 2202 12 1.530368545011953 2202 13 1.3182108574586735 2202 14 2.3118956139018394 2202 15 1.8207629680586095 2202 16 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1.9724245650852694 2205 17 2.4145474375077285 2205 18 2.8327345554092815 2205 19 1.8949654316810438 2205 20 1.8681771080065488 2205 21 1.799767403383676 2205 22 2.1453527860232313 2205 23 2.4189368807621108 2205 24 2.0314113189291305 2205 25 2.0477017057388025 2206 1 0.7880663798225065 2206 2 1.0739897968424543 2206 3 0.8544189226679038 2206 4 1.3941450997544784 2206 5 1.229701254254632 2206 6 0.878438165775421 2206 7 0.5668288528502288 2206 8 0.5475352019704047 2206 9 0.7188219340294992 2206 10 0.5009048390659743 2206 11 0.5492993983048535 2206 12 0.7073268829083474 2206 13 0.5562882157819615 2206 14 0.8441872843292728 2206 15 0.8052764207818299 2206 16 1.1167369225578057 2206 17 1.0376027406928543 2206 18 1.3212121648127066 2206 19 1.0430711696065487 2206 20 0.802014013084019 2206 21 0.7361275630751365 2206 22 0.3232760280917688 2206 23 -4.316951003366476 2206 24 -1.2834398657055246 2206 25 -0.9499824442967082 2207 1 0.9494190342582414 2207 2 1.2811407537614627 2207 3 0.9802407635915742 2207 4 1.7192200022298998 2207 5 1.518673449932134 2207 6 0.9219918484736453 2207 7 0.9976391740922566 2207 8 0.9187688060598944 2207 9 -0.014466755083091698 2207 10 1.0940158567251217 2207 11 0.9936547543022911 2207 12 1.121746407132406 2207 13 0.9334060208898975 2207 14 1.776478269196602 2207 15 1.3564940053140333 2207 16 1.633671386368988 2207 17 2.11411167171876 2207 18 2.708019633951743 2207 19 1.8069735353957128 2207 20 1.4579406356573585 2207 21 1.2950174355732074 2207 22 0.9782486770294696 2207 23 3.022934217078337 2207 24 2.2019739919478596 2207 25 2.193425728786508 2208 1 0.3507616491998107 2208 2 0.9277663738300461 2208 3 0.7479915792522611 2208 4 -1.7086652934054847 2208 5 0.40440687870331615 2208 6 0.46121919724349425 2208 7 0.613246799687353 2208 8 0.7004807363458738 2208 9 0.9148061000622292 2208 10 1.3742092432151125 2208 11 0.9834589957378554 2208 12 1.0549099845535892 2208 13 0.8705895362209901 2208 14 1.768743745941947 2208 15 1.199208407551275 2208 16 1.5891921815549281 2208 17 2.0285145331896146 2208 18 2.610019563205661 2208 19 1.766350408086062 2208 20 1.454102972941378 2208 21 1.341547756510721 2208 22 1.7717318186548 2208 23 3.3470938993035944 2208 24 2.216421173756963 2208 25 2.2789175805027284 2209 1 0.22275579746450802 2209 2 0.8759926738194288 2209 3 0.6701857011326666 2209 4 -2.033699373113926 2209 5 0.23615758362506156 2209 6 0.3885482812045282 2209 7 0.5426925258076949 2209 8 0.6467693997885107 2209 9 0.8439788667553927 2209 10 0.8356680156162923 2209 11 0.9402359508834905 2209 12 1.0030918817127699 2209 13 0.8933700114170762 2209 14 1.8715723349294038 2209 15 1.2341062435031986 2209 16 1.542442007536593 2209 17 1.7359950157861732 2209 18 2.1442791352867943 2209 19 1.250510824571741 2209 20 1.1940636968147587 2209 21 1.108149320477068 2209 22 0.38179817621770695 2209 23 2.6408822222172788 2209 24 1.7479795736677275 2209 25 2.092436097233694 2210 1 0.5285444907652703 2210 2 0.5568621744507567 2210 3 0.5651196660698474 2210 4 1.2914418162049266 2210 5 1.2482546129412704 2210 6 0.7529969069985043 2210 7 0.5675756818463071 2210 8 0.6055390927068984 2210 9 0.4867568233408761 2210 10 1.13227562072084 2210 11 0.6554667916373376 2210 12 0.7291447152648018 2210 13 0.7842134061227171 2210 14 0.900720742577974 2210 15 0.6746609325290791 2210 16 0.9888505859052614 2210 17 1.5820677107484684 2210 18 2.2783821670402915 2210 19 1.4077034334862688 2210 20 1.2140791568044733 2210 21 1.0424883784080166 2210 22 0.45378891122743426 2210 23 2.7106268572982515 2210 24 1.7930569974062394 2210 25 2.125206048735535 2211 1 -0.5279968349391808 2211 2 0.06231805628869225 2211 3 -0.05457141257423004 2211 4 0.6215658174990146 2211 5 -3.10245375742749 2211 6 -0.970754595959054 2211 7 -0.5656307582330058 2211 8 -0.4243930052967748 2211 9 -0.4399683676452142 2211 10 -4.1687822029132455 2211 11 -1.3968892871484861 2211 12 -0.639510135640144 2211 13 -0.4193386391502827 2211 14 -0.12562301820955213 2211 15 -0.1580322715179392 2211 16 -0.42757788665628027 2211 17 0.08481128576126906 2211 18 -5.192835883125554 2211 19 -1.8708957016773518 2211 20 -0.9662210303043608 2211 21 -0.698608317836964 2211 22 -0.412795985654256 2211 23 -4.574118561286891 2211 24 -2.184161939108499 2211 25 -1.6578451552147584 2212 1 0.18808392045400524 2212 2 0.415816507440951 2212 3 0.16195993249237972 2212 4 0.7887259044567436 2212 5 0.49896972393771577 2212 6 0.10903276738812195 2212 7 0.04979659872430631 2212 8 -0.05503536037880252 2212 9 -0.32311457748282635 2212 10 -4.307508258282816 2212 11 -1.4701522162070937 2212 12 -0.5483521114975645 2212 13 -0.17479520195849574 2212 14 0.5259148903598145 2212 15 0.09301736801032234 2212 16 0.23339115027991397 2212 17 0.7711652773433144 2212 18 1.3609466392042435 2212 19 0.6414829256129266 2212 20 0.17474060966841165 2212 21 0.007259939813508254 2212 22 0.24755316491041413 2212 23 1.477397989852868 2212 24 0.8058408738212535 2212 25 0.9236108658440116 2213 1 -1.7916461632468845 2213 2 -4.481026878975552 2213 3 -2.272748180529099 2213 4 -4.137002078220384 2213 5 -1.8428919338490743 2213 6 -1.5183251400804352 2213 7 -1.2165912258153924 2213 8 -1.0380341628136343 2213 9 -1.0519040946058027 2213 10 -1.4027485801089234 2213 11 -1.2813709570449845 2213 12 -1.1056382110103584 2213 13 -1.6160907275866627 2213 14 -6.865304397332469 2213 15 -3.320390881311166 2213 16 -2.3938409577013076 2213 17 -1.2922135383446935 2213 18 -0.43338107914460267 2213 19 -1.361628515040504 2213 20 -1.4100791489620121 2213 21 -1.7292174769382491 2213 22 -1.595625423947908 2213 23 -6.658909833106012 2213 24 -4.0319415488999475 2213 25 -3.2260524970255844 2214 1 -0.7309089231959263 2214 2 -0.5646197962212405 2214 3 -0.6781927369272758 2214 4 -0.5104961427026495 2214 5 -0.7239329146475946 2214 6 -0.8873031619033895 2214 7 -1.0741028701830793 2214 8 -1.0107646598688511 2214 9 -1.0106510338793555 2214 10 -0.6392836071282204 2214 11 -1.016289901526143 2214 12 -1.0013024946316875 2214 13 -1.1415788459381915 2214 14 -0.772320805518028 2214 15 -1.019089702745461 2214 16 -0.46251613524982815 2214 17 -0.23272981911485857 2214 18 0.2757567923744304 2214 19 -0.923812740979244 2214 20 -0.8588452948165255 2214 21 -1.3217349857342953 2214 22 -0.6775635419259061 2214 23 0.8754128144494899 2214 24 -0.10509968498053723 2214 25 -0.23262710760228344 2215 1 -3.0531854299798624 2215 2 -5.052146634542122 2215 3 -3.2309037866137267 2215 4 -4.822268954775076 2215 5 -6.0013795320669345 2215 6 -3.1222571431162756 2215 7 -2.517101562627964 2215 8 -2.6969338719878095 2215 9 -2.4611483812609976 2215 10 -3.8255501079586267 2215 11 -2.916666261001925 2215 12 -2.60520716124903 2215 13 -2.599441342579567 2215 14 -1.902457981955596 2215 15 -2.37226605974222 2215 16 -3.134968607422479 2215 17 -8.065029903724405 2215 18 -4.307616792791407 2215 19 -3.2980478754642633 2215 20 -3.0699283576846597 2215 21 -2.845368912134969 2215 22 -2.7220241602689406 2215 23 -7.550129687934955 2215 24 -5.051309292250476 2215 25 -4.797616418755599 2216 1 -2.586376926667839 2216 2 -2.0823026731676997 2216 3 -2.329897852698906 2216 4 -5.211887113089244 2216 5 -3.241843563546464 2216 6 -2.920904786382458 2216 7 -2.8150504626063864 2216 8 -2.6935418075869393 2216 9 -2.828970144760845 2216 10 -2.6805323948620696 2216 11 -3.263214725579946 2216 12 -4.293741653891804 2216 13 -3.5823213078237295 2216 14 -9.805896971135056 2216 15 -6.24040596664727 2216 16 -4.4992536126296265 2216 17 -3.4456752091768728 2216 18 -2.1560662420571504 2216 19 -2.8788469593870865 2216 20 -3.4041456058559305 2216 21 -3.689642019474757 2216 22 -3.087741451848377 2216 23 -2.0857454483599653 2216 24 -2.6379400894845126 2216 25 -2.4051747148256766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106471.0 3 3.0 4 894.0 5 5188.0 6 6519.0 7 5761.0 8 5367.0 9 4871.0 10 4626.0 11 4487.0 12 4337.0 13 4249.0 14 4347.0 15 4598.0 16 4688.0 17 4957.0 18 5576.0 19 6689.0 20 8389.0 21 10371.0 22 12595.0 23 15418.0 24 18266.0 25 21170.0 26 24112.0 27 27214.0 28 30767.0 29 36485.0 30 42709.0 31 49314.0 32 63098.0 33 80307.0 34 103586.0 35 135435.0 36 189221.0 37 258284.0 38 555435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3008950586155 18.13470084956535 12.166713519011681 25.39769057280747 2 14.515452706717086 19.50532892634306 41.5549011322762 24.424317234663654 3 19.61371450752728 23.83530752770958 28.605435812366732 27.945542152396403 4 11.15791516617584 16.060653337429358 38.74609094998293 34.035340546411874 5 12.77234565359472 37.899212873678586 35.95764176289274 13.370799709833959 6 32.88265518082429 36.258828133166446 17.945306448457103 12.913210237552164 7 29.02433127718165 30.900920363299328 21.44024607348647 18.634502286032554 8 25.522624735176308 36.91894250645441 20.055677305359946 17.502755453009343 9 26.53770644375798 15.290106890232986 19.519985760835652 38.65220090517339 10 14.065901190034507 29.40756698099247 34.856548192215556 21.66998363675746 11 34.869139810424 22.718832052303334 23.30438194814482 19.107646189127845 12 23.148033490881353 24.897411527325925 31.54283949882683 20.411715482965892 13 29.04045263640335 21.425450880855955 25.368651551984307 24.165444930756387 14 21.143603049179273 21.72609921626229 27.827566982234565 29.302730752323875 15 23.937374090422242 29.915043534291897 22.914229779149643 23.233352596136218 16 22.448587930426317 28.032805515975507 27.72129356530279 21.797312988295385 17 22.44238788911327 28.05121576872705 27.827827613177703 21.678568728981972 18 22.145571523607302 27.15875593217977 29.564327530835545 21.13134501337739 19 23.344606440253784 27.289854310998358 27.523072518531468 21.842466730216394 20 23.39163918809602 27.21029831247356 27.982628861585734 21.41543363784468 21 23.116364273847108 26.782444972334048 27.361443722661743 22.7397470311571 22 22.98848232888361 27.580752911050894 26.98781964191313 22.44294511815237 23 22.231199152939915 26.80891557545134 28.64939486134752 22.31049041026122 24 22.53890177482813 27.626184800453814 27.71443829279356 22.120475131924493 25 22.981211948351625 26.809323042544932 27.372471354708377 22.836993654395066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 174.0 1 174.0 2 274.0 3 374.0 4 374.0 5 374.0 6 781.5 7 1189.0 8 1189.0 9 1189.0 10 1871.0 11 2553.0 12 2553.0 13 2553.0 14 4741.0 15 6929.0 16 6929.0 17 6929.0 18 12766.0 19 18603.0 20 18603.0 21 18603.0 22 30016.0 23 41429.0 24 41429.0 25 41429.0 26 59209.5 27 76990.0 28 76990.0 29 76990.0 30 100656.0 31 124322.0 32 124322.0 33 124322.0 34 145586.0 35 166850.0 36 166850.0 37 166850.0 38 186126.5 39 205403.0 40 205403.0 41 205403.0 42 220382.0 43 235361.0 44 235361.0 45 235361.0 46 251270.5 47 267180.0 48 267180.0 49 267180.0 50 254862.5 51 242545.0 52 242545.0 53 242545.0 54 214509.0 55 186473.0 56 186473.0 57 186473.0 58 158722.5 59 130972.0 60 130972.0 61 130972.0 62 105924.5 63 80877.0 64 80877.0 65 80877.0 66 62368.5 67 43860.0 68 43860.0 69 43860.0 70 32108.0 71 20356.0 72 20356.0 73 20356.0 74 14324.5 75 8293.0 76 8293.0 77 8293.0 78 5775.5 79 3258.0 80 3258.0 81 3258.0 82 2252.5 83 1247.0 84 1247.0 85 1247.0 86 836.0 87 425.0 88 425.0 89 425.0 90 267.5 91 110.0 92 110.0 93 110.0 94 67.0 95 24.0 96 24.0 97 24.0 98 15.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0960443862270635 2 0.047593423532160936 3 0.03510550947473583 4 0.031139390847055744 5 0.035641471451449344 6 0.017097187057161414 7 0.01849068819661658 8 0.02208163344059719 9 0.028191599975131362 10 0.03473033609103636 11 0.020259362719771207 12 0.01827630340593117 13 0.015489301127020846 14 0.020580939905799324 15 0.020259362719771207 16 0.02068813230114203 17 0.023635923173066408 18 0.018383495801273873 19 0.03205052620746874 20 0.02202803724292584 21 0.027602041800746487 22 0.03590945243980611 23 0.028352388568145422 24 0.03869645471871643 25 0.030871409858698982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1865804.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.58566026687018 #Duplication Level Percentage of deduplicated Percentage of total 1 88.71046771266366 70.60081145495205 2 8.263274056491634 13.152762435039705 3 1.6579458082883785 3.95846135517961 4 0.544843494191956 1.7344691690950185 5 0.247689970445678 0.9856284919700422 6 0.14238498982129663 0.6799082056211688 7 0.09547672511180531 0.5319004745699043 8 0.06388104058241009 0.40672118346286684 9 0.0456106693393558 0.3266959711127871 >10 0.20319012101195402 2.857448095182974 >50 0.013988361096068559 0.775092368665821 >100 0.009066560781897524 1.5709039775489388 >500 0.0010902450869650338 0.6100866217886016 >1k 9.539644510943978E-4 1.1758673973548606 >5k 1.3628063587062825E-4 0.6332427984555968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 6428 0.34451635863145325 No Hit CCCATGTACTCTGCGTTGATACCAC 5249 0.28132644157692877 No Hit GAGTACATGGGAAGCAGTGGTATCA 2609 0.13983247972455842 No Hit GTATCAACGCAGAGTACATGGGAAG 1884 0.10097523641282792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.3596197671352404E-5 0.0 13 0.0 0.0 0.0 5.3596197671352404E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 1100 0.0 12.017334 1 TTAGGCC 95 1.0375461E-6 12.002474 4 GTAGGAC 1125 0.0 11.31637 3 TGTAGGA 1230 0.0 11.277536 2 TAGGACC 595 0.0 11.178775 4 GCGTTTA 120 1.2790406E-7 11.08116 13 CTAATCC 105 3.4638324E-6 10.857924 3 AGGACGT 835 0.0 10.810031 5 TCCTACA 1215 0.0 10.634796 2 CTGTAGG 1210 0.0 10.610465 1 GGCGAGG 405 0.0 10.555466 19 GCCGGTT 90 9.540625E-5 10.552919 11 GGACGTG 835 0.0 10.466697 6 TAGGACA 445 0.0 10.462831 4 TAGGACG 845 0.0 10.345326 4 CGTTTAT 120 1.5276783E-6 10.289648 14 GATATAC 420 0.0 10.189414 1 TGCACCG 75 0.0026446488 10.134878 5 ATTATAC 225 0.0 10.134064 3 GACGTGG 480 0.0 10.093123 7 >>END_MODULE