FastQCFastQC Report
Thu 26 May 2016
SRR1513108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865804
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC77370.41467378138325356No Hit
CCCATGTACTCTGCGTTGATACCAC71990.38583902703606593No Hit
GAGTACATGGGAAGCAGTGGTATCA28970.1552681846539079No Hit
GTATCAACGCAGAGTACTTTTTTTT27900.1495333915030732No Hit
CATGTACTCTGCGTTGATACCACTG21300.11415990103998061No Hit
GTATCAACGCAGAGTACATGGGAAG20550.1101401862146292No Hit
ACGCAGAGTACATGGGAAGCAGTGG19720.10569170180790693No Hit
TATCAACGCAGAGTACTTTTTTTTT19400.10397662348242366No Hit
GCGTTGATACCACTGCTTCCCATGT18730.10038567823844305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6100.012.01446154
TGTCGAG951.0515614E-611.98914318
GTAGGAC11200.011.8124553
AGGACCG657.8986544E-411.7143995
TACACGG600.00579440611.1042755
TACACCG951.3323146E-511.0207835
TGTAGGA12800.010.7154732
TGGCGAG8250.010.69940318
AGGACCT9550.010.5644265
GGACCTG8550.010.2415366
TCCAACG1409.817995E-810.16936318
AAGCTCG750.002606847610.1524795
ATACGGC750.002663798410.12606715
AGGACGG856.480797E-410.0778285
GACCGCT951.665602E-49.9872047
GGCGAGA5900.09.97214819
GTCCTAA6050.09.7641721
GGCGAGG3500.09.760756519
TATGGCG8950.09.75836116
CTAGCAC2054.0017767E-119.7500943
Produced by FastQC (version 0.11.5)