FastQCFastQC Report
Thu 26 May 2016
SRR1513107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166621
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC123410.5695966207287754No Hit
CCCATGTACTCTGCGTTGATACCAC100590.4642713238725185No Hit
GAGTACATGGGAAGCAGTGGTATCA52510.24235895433488366No Hit
GCGTTGATACCACTGCTTCCCATGT35080.1619111048955955No Hit
GTATCAACGCAGAGTACATGGGAAG33630.15521865614706035No Hit
CATGTACTCTGCGTTGATACCACTG33250.15346477302675454No Hit
TATCAACGCAGAGTACATGGGAAGC26300.1213871738527412No Hit
ACGCAGAGTACATGGGAAGCAGTGG25870.11940251663765836No Hit
GCTTCCCATGTACTCTGCGTTGATA24900.11492549919898312No Hit
GTATCAACGCAGAGTACTTTTTTTT23770.10971000465702123No Hit
ACTCTGCGTTGATACCACTGCTTCC22030.10167906615877904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGT500.001502216213.2972539
GCGTTTA1700.012.85117713
CGAATTT604.0963298E-412.66551215
TAGGACC5900.011.7564234
TGGCGAG10100.011.38041418
GGCGAGG4500.010.97703119
ATGGCGA10950.010.93101517
CCTAGAC2000.010.9270323
TATGGCG10550.010.89524516
TTAGGAC7000.010.8591623
GTAGGAC11650.010.847513
AGGACGT8800.010.6887465
TGTAGGA11850.010.6639382
TGCGTTT2050.010.65658212
CTAACAC2350.010.5125933
GTCTAGA2450.010.4831991
TAGGACT3450.010.4657144
AGGACCT8900.010.4618945
AACACCG1002.3964802E-510.451225
GGACGTG9000.010.4488066
Produced by FastQC (version 0.11.5)