##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513107_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166621
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.397667150830717	31.0	30.0	34.0	25.0	34.0
2	29.09177931904103	31.0	30.0	34.0	16.0	34.0
3	29.46508641797527	31.0	30.0	34.0	23.0	34.0
4	32.34287307286323	35.0	33.0	37.0	19.0	37.0
5	32.56729026442557	35.0	33.0	37.0	23.0	37.0
6	33.02207954229189	37.0	35.0	37.0	27.0	37.0
7	33.0299969399355	37.0	35.0	37.0	27.0	37.0
8	33.01389536979472	37.0	35.0	37.0	26.0	37.0
9	34.394576162605276	39.0	35.0	39.0	27.0	39.0
10	33.90491276508443	39.0	34.0	39.0	22.0	39.0
11	34.27482425398812	39.0	35.0	39.0	26.0	39.0
12	34.117616325144084	39.0	35.0	39.0	25.0	39.0
13	34.19905881093186	39.0	35.0	39.0	26.0	39.0
14	34.52597477823763	40.0	34.0	41.0	17.0	41.0
15	35.0607494342573	40.0	34.0	41.0	25.0	41.0
16	34.6453726793934	40.0	34.0	41.0	18.0	41.0
17	34.180484265591446	39.0	33.0	41.0	17.0	41.0
18	33.59746766970319	39.0	32.0	41.0	10.0	41.0
19	34.49227299098458	39.0	34.0	41.0	18.0	41.0
20	34.75274355782576	39.0	34.0	41.0	19.0	41.0
21	34.77527910972893	40.0	35.0	41.0	18.0	41.0
22	34.36302565146373	40.0	34.0	41.0	13.0	41.0
23	32.68996561927536	39.0	31.0	41.0	8.0	41.0
24	33.78797814661632	39.0	33.0	41.0	2.0	41.0
25	33.52456751780768	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6973323251143739
1101	2	1.099549230061939
1101	3	0.8278671981141059
1101	4	0.7852563725371908
1101	5	0.9788414690297742
1101	6	0.7343940140434029
1101	7	0.8114635109682169
1101	8	0.774631275088808
1101	9	0.902953530060806
1101	10	1.2057086712143956
1101	11	0.8571336696900076
1101	12	0.8324406747766631
1101	13	0.8458881538758263
1101	14	0.02920136818510599
1101	15	0.6128849814558137
1101	16	1.1823182669565
1101	17	1.8425209545294976
1101	18	-4.447301516390546
1101	19	-0.781817236281583
1101	20	0.29235070370931027
1101	21	0.7362553614994525
1101	22	1.2828062983432247
1101	23	3.0452433807435213
1101	24	1.9659909424003104
1101	25	2.0578353238373595
1102	1	1.0189300858491563
1102	2	1.2235413627828748
1102	3	1.0026025799781095
1102	4	1.607930542818245
1102	5	1.3990370562241523
1102	6	0.8824825300244754
1102	7	0.8501338506480707
1102	8	0.8948716953184856
1102	9	0.9956627352659808
1102	10	1.3865555136485312
1102	11	1.014582478678733
1102	12	1.1513817893217535
1102	13	0.9881178804700781
1102	14	1.8380362983283405
1102	15	1.342646616310347
1102	16	1.7418619170398628
1102	17	1.4421991681177175
1102	18	1.7138192640338374
1102	19	1.5208536723819464
1102	20	1.416753947977
1102	21	1.4159606378003176
1102	22	1.8539395816347835
1102	23	3.2648868037448153
1102	24	2.1871432896758307
1102	25	2.421004502347273
1103	1	0.921963605147301
1103	2	1.1917353333015477
1103	3	1.0421809921356981
1103	4	1.4568707987822975
1103	5	1.401455368697242
1103	6	1.0090098667390919
1103	7	0.925037783851721
1103	8	0.8665610268573332
1103	9	0.8898591233459854
1103	10	1.303104391334088
1103	11	1.0688211825323535
1103	12	1.245160596919817
1103	13	1.042076313212803
1103	14	1.8787534468453657
1103	15	1.381473601432596
1103	16	1.5556076329292736
1103	17	2.0046328678034158
1103	18	1.9763589315506138
1103	19	1.5105522771857878
1103	20	1.4273184318340384
1103	21	1.4224956962891895
1103	22	1.7205019893260598
1103	23	3.072446368163135
1103	24	2.032613254095949
1103	25	2.2802948840624424
1104	1	0.5686211661381186
1104	2	1.0715579479814288
1104	3	0.8461212427950819
1104	4	1.3818639305567544
1104	5	1.2827691839299575
1104	6	0.8355279069534447
1104	7	0.7006198943811128
1104	8	0.6936597000395608
1104	9	0.886692920081579
1104	10	1.231420899950379
1104	11	0.8480384191480823
1104	12	0.9572095103762805
1104	13	0.6653234543748141
1104	14	1.6996203825256515
1104	15	1.065730719219907
1104	16	1.3553743405779812
1104	17	1.8531026011733829
1104	18	2.407078831998689
1104	19	1.6496392026173154
1104	20	1.166605786050674
1104	21	1.2129430499602663
1104	22	1.5526438560155853
1104	23	3.075529438295675
1104	24	2.137837067321783
1104	25	2.1583632597644495
1105	1	0.684092566480242
1105	2	1.1384846228486936
1105	3	0.7735370947242259
1105	4	1.0180056234136288
1105	5	1.0868876762252384
1105	6	0.7171387412551766
1105	7	0.5297397069009335
1105	8	0.5540504477953831
1105	9	0.7414494195262549
1105	10	1.2038131385231097
1105	11	0.6827438182996559
1105	12	0.7869602658073802
1105	13	0.7481210253828436
1105	14	1.5126984300531774
1105	15	0.8959058819116592
1105	16	1.2640145258905022
1105	17	1.7794180355797877
1105	18	1.5116699232064335
1105	19	1.2336990741781335
1105	20	1.0434953315035855
1105	21	0.9210014517171672
1105	22	1.4322979931292679
1105	23	2.859762722386705
1105	24	1.9344917980526546
1105	25	1.8600992375282743
1106	1	-0.5329760506878181
1106	2	-0.17506634496596618
1106	3	-0.29448853881064707
1106	4	-2.2567306344998848
1106	5	-0.5201785385313684
1106	6	-0.5318102386128416
1106	7	-0.5622614906774572
1106	8	-0.4233498639879514
1106	9	-0.36858158302153043
1106	10	-0.23912875241745724
1106	11	-0.34110227780963953
1106	12	-0.3563660388680603
1106	13	-0.3639284492256749
1106	14	0.3724698689246466
1106	15	-0.19336560704491745
1106	16	-1.079987076833234
1106	17	-5.833606773434081
1106	18	-7.118159488838938
1106	19	-3.0391562713547664
1106	20	-1.59396431788943
1106	21	-1.2216755601071014
1106	22	-1.4682594723573388
1106	23	-5.086833986951362
1106	24	-2.0580755148096763
1106	25	-0.5924584377856803
1107	1	0.8048609113718257
1107	2	1.0796294631796464
1107	3	0.7752474151420685
1107	4	1.051818283761797
1107	5	1.1393323735705962
1107	6	0.7334402327377205
1107	7	0.6402771720329525
1107	8	0.6363269555348836
1107	9	0.6178995544812267
1107	10	1.0453865217335903
1107	11	0.6977453834839835
1107	12	0.7634093480083024
1107	13	0.6667574443562785
1107	14	1.4954357831253802
1107	15	0.9722297403686113
1107	16	-5.67704042544122
1107	17	-0.7586004671574145
1107	18	0.8308897838703899
1107	19	0.9009824567614544
1107	20	0.7941114850516371
1107	21	0.9834683675716178
1107	22	0.8549170166468087
1107	23	2.8663230896880947
1107	24	1.9174611169306885
1107	25	1.6813595964706138
1108	1	0.4630119877008312
1108	2	0.6619168552718548
1108	3	0.5230535542399792
1108	4	0.8962577760824573
1108	5	0.741072700660645
1108	6	0.4146567581622733
1108	7	0.30002840212451787
1108	8	0.3427198789046031
1108	9	0.40488254876184726
1108	10	0.8081912601640511
1108	11	0.40374866017540256
1108	12	0.4142050994295303
1108	13	0.3597339808227531
1108	14	0.8827800199339251
1108	15	0.5756817772339602
1108	16	0.8820495332919407
1108	17	1.1518102845684624
1108	18	0.7999130949914033
1108	19	0.6394303253754785
1108	20	0.5073271777141102
1108	21	0.6285525577220383
1108	22	0.9564708249257166
1108	23	2.7245129264024257
1108	24	1.3952727503398634
1108	25	1.5620182885403082
1109	1	0.3756417090811688
1109	2	0.7927167357238254
1109	3	0.6110631223620295
1109	4	0.5377133731913375
1109	5	0.543237119144365
1109	6	0.46748667969283275
1109	7	0.4120531434307111
1109	8	0.24753511086415614
1109	9	0.3990668866861782
1109	10	0.8593156963945248
1109	11	0.5774262314387926
1109	12	0.6558048175358522
1109	13	0.521961582864769
1109	14	-0.5333002598484029
1109	15	0.27044074458436995
1109	16	0.1634185864729787
1109	17	1.141560075395077
1109	18	1.880522062377473
1109	19	1.1336637022435028
1109	20	1.0036294630358782
1109	21	0.8551966462646163
1109	22	0.7311962603380451
1109	23	-4.015091174180551
1109	24	-1.2354278039606896
1109	25	0.1823586081101638
1110	1	0.6070953861000525
1110	2	0.9350412485112649
1110	3	0.7352846907128594
1110	4	1.1508202475741314
1110	5	0.9255100387920834
1110	6	0.572199592085262
1110	7	0.552891330415541
1110	8	0.4821730519754297
1110	9	0.6094449676632223
1110	10	0.9506466381708591
1110	11	0.7229198931409684
1110	12	0.7728769068564745
1110	13	0.6883192281216282
1110	14	1.5127909552103418
1110	15	0.8573580544858217
1110	16	1.4018453987827755
1110	17	1.6641774195575891
1110	18	2.3266112620792043
1110	19	1.4433938459384592
1110	20	0.9907614084600098
1110	21	0.9521050885951752
1110	22	1.1438809174250437
1110	23	2.6645627890190298
1110	24	1.7235647190647683
1110	25	1.9830448371756475
1111	1	0.577009024454977
1111	2	0.8444300893131675
1111	3	0.636096188545185
1111	4	1.196198841116967
1111	5	0.8629413637916841
1111	6	0.4114430650319747
1111	7	0.3204448571600125
1111	8	0.27216272058787894
1111	9	0.2982181589949775
1111	10	0.5301196655888774
1111	11	0.29231032759616227
1111	12	0.36612905050824196
1111	13	0.32048711983801326
1111	14	-0.3045059283766989
1111	15	0.10542831476086434
1111	16	0.5318656203963457
1111	17	1.1848270282062714
1111	18	1.7373244424468979
1111	19	0.8977343111552614
1111	20	0.6292026855850352
1111	21	0.6317725690737817
1111	22	0.9397633413785442
1111	23	0.758954428973766
1111	24	0.7531736686933499
1111	25	0.28299825872532836
1112	1	0.22271480631141927
1112	2	0.5704189851211154
1112	3	0.33819006822158926
1112	4	0.8363946340319686
1112	5	0.6395827348923291
1112	6	0.014913427011990166
1112	7	-0.03920048374968843
1112	8	-0.07747354800634554
1112	9	-0.029529135415081953
1112	10	0.27259608562097526
1112	11	0.015089522736836614
1112	12	-0.03464635734333399
1112	13	-0.021219931605770626
1112	14	0.32675091612166085
1112	15	0.0762747778998829
1112	16	0.40965777311186
1112	17	0.6664492145806236
1112	18	0.7644004727452085
1112	19	0.18997090533287775
1112	20	0.16115340788091714
1112	21	0.10714898293240793
1112	22	0.14262487851866723
1112	23	1.860977548615594
1112	24	0.8054580871257215
1112	25	0.7118369486614995
1113	1	0.4572889539709095
1113	2	0.8642773548781335
1113	3	0.5315854288881994
1113	4	0.7248406448187978
1113	5	0.8391424551007276
1113	6	0.36934644326041166
1113	7	0.36281255863988093
1113	8	0.3286848465283967
1113	9	0.4864483162028179
1113	10	0.707420845513056
1113	11	0.4084306903312438
1113	12	0.4302319597905253
1113	13	0.2752913094827676
1113	14	1.0231580317851154
1113	15	0.6949816954726629
1113	16	1.003481434393855
1113	17	1.3083837712429442
1113	18	1.8471914929842796
1113	19	1.0161968126038943
1113	20	0.7299864755201639
1113	21	0.7341166978234313
1113	22	0.9744673970888513
1113	23	0.7919164505561227
1113	24	0.8677729916462766
1113	25	1.2196452084264138
1114	1	-0.351593853409927
1114	2	-3.108814993306506
1114	3	-1.058799763561236
1114	4	0.08509834083507428
1114	5	0.23242991616674402
1114	6	0.13679958752511112
1114	7	0.12121777076215778
1114	8	0.13274553922300214
1114	9	0.08909875070809647
1114	10	0.3485506595162917
1114	11	0.1373959526085784
1114	12	0.19732599419528896
1114	13	0.10477099643394183
1114	14	0.8709459848324457
1114	15	0.2826678288027651
1114	16	0.5681599115530815
1114	17	0.9652698211203159
1114	18	1.1869614781859426
1114	19	0.4830419566837918
1114	20	0.28194073661894237
1114	21	0.04180574766782996
1114	22	0.3515722144148725
1114	23	1.9692275254416387
1114	24	0.9352769021898197
1114	25	0.9692957440792327
1115	1	-0.11129373199856829
1115	2	0.17950660735774093
1115	3	-0.024476425941028168
1115	4	0.019394584074113652
1115	5	0.025740229595520248
1115	6	-0.38527990348612917
1115	7	-0.5418569767338113
1115	8	-0.5311867136947725
1115	9	-0.46176810881956243
1115	10	-0.3195691134802914
1115	11	-0.28266962723665046
1115	12	-0.513495369971146
1115	13	-0.48985912764295847
1115	14	0.3100402534499196
1115	15	-0.37279831172450884
1115	16	0.05857189427631937
1115	17	0.4903937267741796
1115	18	0.9161807657800622
1115	19	0.09280895989710558
1115	20	-0.32727638467869014
1115	21	-0.3144020515794992
1115	22	-0.07434401182767658
1115	23	1.6269148252463381
1115	24	0.33580019916453807
1115	25	3.3844355426992934E-4
1116	1	0.09959306927457234
1116	2	0.15260827499895768
1116	3	0.1258376935447174
1116	4	0.7684584740644098
1116	5	0.16171354486749578
1116	6	0.04611052265799742
1116	7	-0.05391771472946516
1116	8	-0.15239656741938745
1116	9	-0.07155641758853193
1116	10	0.33514360450181613
1116	11	-0.022311321198486667
1116	12	0.08339529453967742
1116	13	-0.02345363579757276
1116	14	0.5921492510600785
1116	15	0.020823411890454224
1116	16	0.4581295659042013
1116	17	0.6909100336794083
1116	18	1.3133588588773009
1116	19	0.4746596883426619
1116	20	0.1432425913164721
1116	21	0.016850754436291027
1116	22	0.40931097512772396
1116	23	1.7243097626152917
1116	24	0.7080763774597472
1116	25	0.9043378356806073
1201	1	0.8432023361857546
1201	2	1.2012123575159421
1201	3	0.9406615359116195
1201	4	1.3384753082996426
1201	5	1.2236205421661097
1201	6	0.8337655601180387
1201	7	0.7871017624291099
1201	8	0.8183541638123302
1201	9	0.939930067736114
1201	10	1.2329770779771465
1201	11	0.9172001248777946
1201	12	1.0490041900315816
1201	13	0.9298162295457431
1201	14	1.7141731250664023
1201	15	1.256670789515539
1201	16	1.6042656876334291
1201	17	2.0632044763183472
1201	18	2.5695220116767885
1201	19	1.7333536508877785
1201	20	1.4214482750632698
1201	21	1.2960687276906597
1201	22	1.6000091574086142
1201	23	3.2127109917379997
1201	24	2.1447060569558545
1201	25	2.348772048899349
1202	1	1.295816129507351
1202	2	1.6552504344782228
1202	3	1.423164686342293
1202	4	2.0021646373701785
1202	5	1.7577577576266705
1202	6	1.3222780569890844
1202	7	1.1652283232444347
1202	8	1.214545764668479
1202	9	1.3946601264959853
1202	10	1.904181848746461
1202	11	1.479295533234918
1202	12	1.4992087386013182
1202	13	1.480780474158962
1202	14	2.3575325600156205
1202	15	1.777180122483358
1202	16	2.0859524791770383
1202	17	2.510931245821631
1202	18	3.152625237631881
1202	19	2.398613118678796
1202	20	2.065964885555225
1202	21	1.988037247985993
1202	22	2.3299155211809506
1202	23	3.810545092616607
1202	24	2.569204087033633
1202	25	2.8328210288705407
1203	1	0.8054874950473412
1203	2	1.1694983255139135
1203	3	0.8116272348526934
1203	4	1.5060850150410303
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2211	15	-0.2761028051909733
2211	16	-0.484662739504806
2211	17	0.18734217299147105
2211	18	-5.166218462059405
2211	19	-1.681203814694257
2211	20	-0.9606894442647871
2211	21	-0.8067148082155171
2211	22	-0.2857150533232442
2211	23	-4.774289392856428
2211	24	-1.9627535646744256
2211	25	-1.2233121670323541
2212	1	-0.014744342048093273
2212	2	0.17665415278172958
2212	3	-0.005078089772425187
2212	4	0.36380302468803194
2212	5	0.3306586329929857
2212	6	-0.13483885452620825
2212	7	-0.18358327449029588
2212	8	-0.2911787132054968
2212	9	-0.4895334061923933
2212	10	-3.936308133920388
2212	11	-1.367867182885206
2212	12	-0.6982626957253473
2212	13	-0.43112149637577346
2212	14	0.33086074499626505
2212	15	-0.1099304441537825
2212	16	0.06305392733876403
2212	17	0.6197721602598136
2212	18	1.1097314462817778
2212	19	0.3232750087572569
2212	20	-0.07444316987866273
2212	21	-0.06435190600463869
2212	22	0.13336160599960323
2212	23	1.3321470426237951
2212	24	0.48067876624954664
2212	25	0.4476346910690765
2213	1	-1.4879215378242812
2213	2	-3.846787964915187
2213	3	-1.855190566720001
2213	4	-3.311303042689037
2213	5	-1.2409240798393704
2213	6	-1.004945163180075
2213	7	-1.0005181360498625
2213	8	-0.8527757557814084
2213	9	-0.7177506224456351
2213	10	-0.7676883840509916
2213	11	-0.8492639027796329
2213	12	-0.7167632968192734
2213	13	-1.1613744593597133
2213	14	-6.051914105989088
2213	15	-2.6752261131304067
2213	16	-1.9597145455469729
2213	17	-0.7480354728987493
2213	18	0.001263386084261242
2213	19	-0.6504617393770431
2213	20	-0.7975257222094996
2213	21	-0.9400159731760951
2213	22	-0.774053228536971
2213	23	-5.257625926402557
2213	24	-2.911599285324943
2213	25	-2.1207509993287346
2214	1	-0.002142534435758847
2214	2	0.21164029106559923
2214	3	0.003316652963942346
2214	4	0.31590117413552576
2214	5	0.13528016751239136
2214	6	-0.07888693762473764
2214	7	-0.3327005259229736
2214	8	-0.20148109702467565
2214	9	-0.08798252744497859
2214	10	0.12977073493387792
2214	11	-0.2793393049755579
2214	12	-0.10025947583080352
2214	13	-0.13995046701187164
2214	14	0.4436144725624942
2214	15	0.08895855610732895
2214	16	0.4746721120293742
2214	17	0.5922834346343464
2214	18	1.1608832206268147
2214	19	0.19213958550364652
2214	20	0.13073819565196487
2214	21	-0.06251228447217727
2214	22	0.48002998862339297
2214	23	1.8651101252087727
2214	24	0.8799308399383534
2214	25	0.6466084992110481
2215	1	-2.3987719159524943
2215	2	-4.665825609590765
2215	3	-2.504801308844165
2215	4	-4.090268624814321
2215	5	-4.89392000029833
2215	6	-2.383551713665895
2215	7	-1.8217363934973285
2215	8	-2.1385675030180096
2215	9	-1.7889276040656839
2215	10	-3.0959501152336557
2215	11	-2.0819186470977016
2215	12	-1.7826713850398548
2215	13	-1.9199845329035483
2215	14	-1.2330654253342814
2215	15	-1.8446114596111585
2215	16	-2.557696073513249
2215	17	-6.855287127334801
2215	18	-3.1817802157621387
2215	19	-2.279543142034548
2215	20	-2.091950963318787
2215	21	-1.8916479096969994
2215	22	-1.7409336007000107
2215	23	-6.3716411316006685
2215	24	-3.7221074464087636
2215	25	-3.322562892051618
2216	1	-0.983035267860604
2216	2	-0.39559887882693445
2216	3	-0.8248393985327205
2216	4	-3.0389738282796905
2216	5	-1.269161299375142
2216	6	-0.9590341295313891
2216	7	-1.1973290820519153
2216	8	-0.9379881835531592
2216	9	-1.0706091663882944
2216	10	-0.7429973033498953
2216	11	-1.2174985149783595
2216	12	-1.9715063838662275
2216	13	-1.3372401454002372
2216	14	-6.872982432259214
2216	15	-3.4075000921072522
2216	16	-1.8278384863162955
2216	17	-0.9866979491068051
2216	18	-0.2896357641244265
2216	19	-0.7440463665178143
2216	20	-1.1822750468089254
2216	21	-1.508451711343298
2216	22	-0.9768195730303759
2216	23	0.247985343711548
2216	24	-0.4726688550942768
2216	25	-0.42661024725239116
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96572.0
3	3.0
4	872.0
5	4986.0
6	6157.0
7	5537.0
8	5149.0
9	4982.0
10	4691.0
11	4603.0
12	4475.0
13	4445.0
14	4323.0
15	4490.0
16	4622.0
17	4951.0
18	5558.0
19	6667.0
20	8148.0
21	10429.0
22	12786.0
23	15542.0
24	18391.0
25	21671.0
26	24726.0
27	28402.0
28	31863.0
29	38062.0
30	44957.0
31	51644.0
32	66608.0
33	87626.0
34	114679.0
35	151309.0
36	212599.0
37	298477.0
38	755619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.763946237075864	17.76493322145046	11.973608333548967	25.49751220792471
2	14.067403402678627	19.701115817043686	41.21240974737577	25.01907103290192
3	19.445140861750623	24.29700712931246	28.73203878205616	27.525813226880754
4	10.920252933126742	16.157082256822417	38.55189505934584	34.370769750705
5	12.621227882332137	37.72096890887172	36.01661049957546	13.641192709220684
6	33.070213665665214	35.531588209333634	17.973047584050878	13.425150540950279
7	28.852338081173386	30.878672020489546	21.61909132121183	18.64989857712524
8	26.164380462848364	36.01406946741831	20.132458708218284	17.68909136151505
9	26.92704384977558	15.103190879350098	19.367484369705068	38.60228090116926
10	14.407606439784121	29.063060259510245	34.548114850111894	21.98121845059374
11	34.76223230649204	22.550237975431518	23.125718427284774	19.561811290791667
12	23.46809233547596	24.888283011446838	31.048828633525805	20.594796019551396
13	29.165653024699935	21.08876918553041	25.13256590442329	24.61301188534636
14	20.997384779091156	21.45505997927212	27.976308360628117	29.5712468810086
15	24.77069937843697	28.8136751252913	22.770747389841524	23.644878106430202
16	22.478510160931425	27.630808719658006	27.53312343615833	22.357557683252235
17	22.624365660341123	27.770365350096398	27.71367182017625	21.89159716938623
18	22.253628798276072	26.69645136466266	29.430947002903224	21.618972834158047
19	23.682538685853693	26.960147891784874	27.48417753662667	21.873135885734758
20	23.85668272140512	26.756138017506792	27.405313898374565	21.98186536271352
21	23.221542322597443	26.34581806329253	27.402694175362395	23.02994543874763
22	23.167672525441972	27.067320278639272	26.43379316108377	23.33121403483499
23	21.17019665634355	26.72662940002179	29.06609012323108	23.03708382040358
24	23.050327184880235	27.408359606011413	26.974237076520446	22.56707613258791
25	22.938502977479377	26.346744994981364	26.956091759367585	23.758660268171674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	190.0
2	335.0
3	480.0
4	480.0
5	480.0
6	981.0
7	1482.0
8	1482.0
9	1482.0
10	2322.0
11	3162.0
12	3162.0
13	3162.0
14	5587.5
15	8013.0
16	8013.0
17	8013.0
18	14576.5
19	21140.0
20	21140.0
21	21140.0
22	33659.0
23	46178.0
24	46178.0
25	46178.0
26	65534.0
27	84890.0
28	84890.0
29	84890.0
30	109880.0
31	134870.0
32	134870.0
33	134870.0
34	158248.5
35	181627.0
36	181627.0
37	181627.0
38	203833.0
39	226039.0
40	226039.0
41	226039.0
42	246750.5
43	267462.0
44	267462.0
45	267462.0
46	295744.0
47	324026.0
48	324026.0
49	324026.0
50	307533.5
51	291041.0
52	291041.0
53	291041.0
54	255654.5
55	220268.0
56	220268.0
57	220268.0
58	188600.0
59	156932.0
60	156932.0
61	156932.0
62	128188.5
63	99445.0
64	99445.0
65	99445.0
66	77620.0
67	55795.0
68	55795.0
69	55795.0
70	40966.5
71	26138.0
72	26138.0
73	26138.0
74	18364.5
75	10591.0
76	10591.0
77	10591.0
78	7533.5
79	4476.0
80	4476.0
81	4476.0
82	3061.0
83	1646.0
84	1646.0
85	1646.0
86	1107.5
87	569.0
88	569.0
89	569.0
90	348.0
91	127.0
92	127.0
93	127.0
94	76.0
95	25.0
96	25.0
97	25.0
98	17.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11321777089763276
2	0.0476779279809436
3	0.035723829871491135
4	0.03106219315699423
5	0.03516997204402616
6	0.019985036607694653
7	0.020446584797248805
8	0.023631267305172433
9	0.029308310036688465
10	0.03563152023358031
11	0.019892726969783826
12	0.019985036607694653
13	0.01569263844484107
14	0.021046597443669195
15	0.021646610090089592
16	0.02220046791755457
17	0.02718518836473938
18	0.018831166133809283
19	0.03290838591521083
20	0.022615861288153304
21	0.02838521365758017
22	0.03738540335388607
23	0.028108284743847682
24	0.04029315694807722
25	0.032077599174013365
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2166621.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.556350728922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.46837938197491	61.28717359942216
2	10.547339975115111	15.305529969832628
3	2.6898354933764037	5.8549394238156385
4	0.9571297401340735	2.7778336447299927
5	0.45121969182635835	1.6369427107974681
6	0.23794535230391312	1.035866786164778
7	0.13806547653153353	0.7012268997144389
8	0.09534479871556964	0.5534296524628275
9	0.07097542947827688	0.4634746338925554
>10	0.31161668731426234	3.877461274270581
>50	0.01783623269612066	0.8989855056691552
>100	0.010962088450073924	1.5179658705068588
>500	0.0016748260417095901	0.8786777673128412
>1k	0.0014815768830503029	1.9181348707718402
>5k	6.441638621957839E-5	0.24542217851907736
>10k+	1.2883277243915678E-4	1.0469352121171838
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	12341	0.5695966207287754	No Hit
CCCATGTACTCTGCGTTGATACCAC	10059	0.4642713238725185	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5251	0.24235895433488366	No Hit
GCGTTGATACCACTGCTTCCCATGT	3508	0.1619111048955955	No Hit
GTATCAACGCAGAGTACATGGGAAG	3363	0.15521865614706035	No Hit
CATGTACTCTGCGTTGATACCACTG	3325	0.15346477302675454	No Hit
TATCAACGCAGAGTACATGGGAAGC	2630	0.1213871738527412	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2587	0.11940251663765836	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2490	0.11492549919898312	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2377	0.10971000465702123	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2203	0.10167906615877904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	4.615481895541491E-5	0.0	0.0	0.0	0.0
4	4.615481895541491E-5	0.0	0.0	0.0	0.0
5	4.615481895541491E-5	0.0	0.0	0.0	0.0
6	4.615481895541491E-5	0.0	0.0	0.0	0.0
7	9.230963791082982E-5	0.0	0.0	0.0	0.0
8	9.230963791082982E-5	0.0	0.0	0.0	0.0
9	9.230963791082982E-5	0.0	0.0	1.384644568662447E-4	0.0
10	9.230963791082982E-5	0.0	0.0	1.384644568662447E-4	0.0
11	9.230963791082982E-5	0.0	0.0	1.384644568662447E-4	0.0
12	9.230963791082982E-5	0.0	0.0	1.8461927582165964E-4	0.0
13	9.230963791082982E-5	0.0	0.0	2.3077409477707453E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGGT	50	0.0015022162	13.297253	9
GCGTTTA	170	0.0	12.851177	13
CGAATTT	60	4.0963298E-4	12.665512	15
TAGGACC	590	0.0	11.756423	4
TGGCGAG	1010	0.0	11.380414	18
GGCGAGG	450	0.0	10.977031	19
ATGGCGA	1095	0.0	10.931015	17
CCTAGAC	200	0.0	10.927032	3
TATGGCG	1055	0.0	10.895245	16
TTAGGAC	700	0.0	10.859162	3
GTAGGAC	1165	0.0	10.84751	3
AGGACGT	880	0.0	10.688746	5
TGTAGGA	1185	0.0	10.663938	2
TGCGTTT	205	0.0	10.656582	12
CTAACAC	235	0.0	10.512593	3
GTCTAGA	245	0.0	10.483199	1
TAGGACT	345	0.0	10.465714	4
AGGACCT	890	0.0	10.461894	5
AACACCG	100	2.3964802E-5	10.45122	5
GGACGTG	900	0.0	10.448806	6
>>END_MODULE