FastQCFastQC Report
Thu 26 May 2016
SRR1513107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166621
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC144380.6663832760782804No Hit
CCCATGTACTCTGCGTTGATACCAC132510.611597505978203No Hit
GAGTACATGGGAAGCAGTGGTATCA56420.2604054885464509No Hit
CATGTACTCTGCGTTGATACCACTG39070.18032687765880603No Hit
GTATCAACGCAGAGTACTTTTTTTT37290.17211131988474218No Hit
GTATCAACGCAGAGTACATGGGAAG36580.16883432773890775No Hit
GCGTTGATACCACTGCTTCCCATGT35740.16495732294665288No Hit
ACGCAGAGTACATGGGAAGCAGTGG33410.15420325013004121No Hit
TATCAACGCAGAGTACATGGGAAGC31490.14534152489060154No Hit
GCTTCCCATGTACTCTGCGTTGATA29430.13583363218578606No Hit
ACTCTGCGTTGATACCACTGCTTCC26810.12374106961946738No Hit
TATCAACGCAGAGTACTTTTTTTTT26620.12286412805931447No Hit
GGTATCAACGCAGAGTACTTTTTTT22410.10343294927908481No Hit
GGTATCAACGCAGAGTACATGGGAA22040.10172522097773445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4300.011.73405554
GTCCTAC11950.011.3220081
GGCGAGG4800.011.06881719
GTAGGAC10200.011.013433
TACGCTG700.00146575810.88009555
GGACGTG9000.010.7894286
GACGTGA5200.010.5827647
TGTAGGA11500.010.5190872
GGACCGT1002.3442864E-510.4720916
AGGACGT9700.010.403395
TCCTACA14450.010.34913352
GTGCGCC1303.913883E-710.217133511
TCGTGTA750.00259081410.1601682
TAGGACT2750.010.0393614
GGACGGA951.6098232E-410.021146
TGGACCG1054.0120383E-59.973425
GACCGTG1252.61262E-69.8675167
TAGGACG9750.09.861834
CTAAATT6950.09.82883619
TGGCGAG10050.09.82000918
Produced by FastQC (version 0.11.5)