FastQCFastQC Report
Thu 26 May 2016
SRR1513106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216105
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22400.10107824313378654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA400.005274551514.2522342
TAGGACC4000.011.1639984
AGTACCG951.3594648E-511.000985
GACCCGT700.001493256810.8559046
GTAGGAC7200.010.8206533
ATAGGAC2300.010.740333
ACTGTTC4400.010.5783398
GTATTAC2100.010.4165051
GTATAAA2950.010.3167151
GTATAGG3000.010.144771
GTATAAT2700.09.862971
TAGTACC1451.6145532E-79.8289064
TTGGACT2151.0913936E-119.7222364
GTGTTAC2350.09.7130781
CTAGCCT2350.09.703434
GTCCTAC6900.09.6485581
ACAGGAC3650.09.6312123
GTATTAT2303.6379788E-129.5107221
GTCTTAG2303.6379788E-129.5107221
TAATACA3200.09.5012754
Produced by FastQC (version 0.11.5)