FastQCFastQC Report
Thu 26 May 2016
SRR1513106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216105
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33830.1526552216614285No Hit
TATCAACGCAGAGTACTTTTTTTTT25560.11533749529015998No Hit
GTACATGGGAAGCAGTGGTATCAAC24330.10978721676093868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG400.00520820514.2794524
TACGCTG657.887956E-411.7164735
TTAGACT2350.010.9374524
GTCCTAA4300.010.8575451
ACAGTCG700.001508151310.8429958
CTAGGAC2400.010.7095883
ATTAGGC1252.1783853E-710.661993
TAGGACG5850.010.5773724
GTAGGAC6700.010.3721393
TGGCGAG6250.010.17371518
GTACCGT750.002603353210.1542766
CCTTACG856.4363313E-410.085552
GGCCGCG856.674834E-410.0457157
GGCGAGG3400.010.04503519
GGACCGT951.6106272E-410.0206686
TACACCG951.6106272E-410.0206685
CCGTGCA2107.2759576E-129.9394129
CCAGGAC7300.09.9108523
ACCGTGC1252.6140406E-69.86712558
TAGGACA3100.09.8267194
Produced by FastQC (version 0.11.5)